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Citations for System Biosciences :
1 - 50 of 126 citations for Yeast Expression Cloning kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... pGreenpuro shRNA cloning and expression lentivector was purchased from System Bioscience (Cat ...
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bioRxiv - Immunology 2022Quote: ORF7a or ORF7b coding sequences (codon-optimized for mammalian expression) were cloned into pLVX-EF1α-IRES-Puro Cloning and Expression Lentivector (System Biosciences) to generate pseudotyped lentiviral particles encoding the ORF7a or ORF7b accessory proteins of SARS-CoV-2 (Wuhan-Hu-1 isolate ...
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bioRxiv - Systems Biology 2020Quote: ... GFP gene was amplified from pGreenPuro shRNA Cloning and Expression Lentivector (CMV; System biosciences, SI505A-1) and sub-cloned into ITR-CAG-DEST-IRES-Neomycin-ITR plasmid (generous gift from Marc Supprian Schmidt) ...
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bioRxiv - Cancer Biology 2022Quote: ... 34kDa isoform of PIM1 in a pCDH-CMV-MCS-EF1α-GreenPuro Cloning and Expression Lentivector(System Biosciences) via the Spinfection method ...
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bioRxiv - Cancer Biology 2019Quote: Overexpression vectors were constructed using pCDH-CMV-MCS-EF1-GFP cloning/ expression vector (System Biosciences, Palo Alto, CA,USA). C/EBPδ insert was cut out of a PCR4 TOPO cloning vector (Invitrogen ...
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bioRxiv - Cancer Biology 2024Quote: ... stable HEK293-F cell lines for each cytokine were prepared through cloning into the expression cassette of PiggyBac (System Biosciences) transposon vector ...
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bioRxiv - Immunology 2023Quote: ... Inserts were introduced into the pCDH-CuO-MCS-EF1a-CymR-T2A-Puro SparQ™ All-in-one Cloning and Expression Lentivector (QM800A-1, System Biosciences). The correct sequence of the gene of interest (GOI ...
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bioRxiv - Developmental Biology 2022Quote: ... pre-digested with XhoI and HindIII using Cold Fusion Cloning kit (System Biosciences). Deletion of putative TF binding sites from those enhancers were performed using QuickChange II XL site-directed mutagenesis kit (Agilent Technologies) ...
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bioRxiv - Cell Biology 2021Quote: ... Not1 digested and Calf Intestinal Phosphatase treated pCDH-CMV-MCS-EF1α-copGFP Dual Promoter Cloning and Expression Lentivector (System Biosciences LLC, #CD511B-1). Similarly ...
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ACE2-lentiviral transduction enables mouse SARS-CoV-2 infection and mapping of receptor interactionsbioRxiv - Microbiology 2021Quote: ACE2 genes were cloned into pCDH-EF1α-MCS-BGH-PGK-GFP-T2A-Puro Cloning and Expression Lentivector (System Biosciences, catalogue number CD550A-1), where the EF1α promoter was replaced with the full length EF1α using NheI and ClaI restriction enzymes (New England Biolabs) ...
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bioRxiv - Genomics 2023Quote: ... Fragments were resuspended at a final concentration of 100ng/ul in EB buffer and then cloned into a PB-CMV-MCS-EF1α-Puro PiggyBac cDNA Cloning and Expression Vector (PB vector hereafter, System Biosciences #PB510B-1). Following ligation ...
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bioRxiv - Genetics 2019Quote: PCR amplification was performed according to the PrecisionX Multiplex gRNA Cloning Kit (System Bioscience). BbsI-linearized piggyBac-U6 backbone was mixed with purified PCE product in a vector:insert 2:1 ratio and was directly used for transformation of One Shot MAX Efficiency DH5α-T1 Competent Cells (Invitrogen).
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bioRxiv - Microbiology 2020Quote: ... Fusion was performed with the Cold Fusion Cloning Kit (SBI System Biosciences, Cat. #MC010B-1).
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bioRxiv - Molecular Biology 2022Quote: ... The siRNAs for CLC3 and CLC7 were constructed using double-promoter pFIV-H1/U6 siRNA cloning and expression vectors (System Biosciences, Palo Alto, CA, USA; Cat: SI111A-1), according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... The cDNA was subsequently cloned into the pLVX tight puro vector using the Cold FusionTM cloning kit (System Biosciences), according to manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: ... using Cold Fusion cloning (System Biosciences, MC010B-1). All constructs were sequence verified for successful insert integration ...
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bioRxiv - Molecular Biology 2020Quote: ... stem-loop sequence was retrieved from www.miRBase.org and used to design oligonucleotides for cloning into the XMIRXpress pre-linearized cloning lentivector (cat. no. XMIRXP-Vect, System Biosciences), according to vendor’s instructions (XMIR-c39-top ...
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bioRxiv - Immunology 2024Quote: ... and Transposase Expression vector DNA (System Biosciences). Cells were cultured with 2 µg/mL puromycin (Life technologies) ...
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bioRxiv - Developmental Biology 2022Quote: ... The amplified coding sequences followed by p2a-tdTomato were cloned downstream of the endothelial specific fli1a promotor into a Tol2 enabled vector using the Cold Fusion Cloning kit (System Biosciences, Cat# MC010B-1).
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bioRxiv - Neuroscience 2022Quote: ... 0N4R tau was replaced with 2N4R tau after inserting 2N4R tau between BamHI and SalI restriction sites using a cold fusion cloning kit (System Biosciences, Catalog # MC100B-1). A Q5 Site-Directed Mutagenesis Kit (New England BioLabs ...
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bioRxiv - Cell Biology 2020Quote: ... along with PiggyBac Transposase expression plasmid (System Biosciences) and selected with 500 μg/ml G418 (Invitrogen) ...
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bioRxiv - Cell Biology 2023Quote: ... and 0.2 µg transposase expression vector (System Biosciences) were used ...
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bioRxiv - Molecular Biology 2020Quote: ... we replaced the KRAB and dCas9 cassettes from the pHR-SFFV-KRAB-dCas9-mCherry vector with the HA-tag-MEOX1 cassette using Cold-fusion cloning kit (SBI System BioSciences, Palo Alto, CA, USA) following the instruction provided by the manufacturer ...
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bioRxiv - Developmental Biology 2020Quote: ... The resulting PB510B-based sgRNA expression vector and PB533-dCas9-EGFP were co-transfected with a transposase expression vector PB200PA-1 (System BioSciences) into MC12 cells ...
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bioRxiv - Cell Biology 2022Quote: ... with a PiggyBac transposon expression vector (PB210PA-1; System Biosciences), or pEGFP-C1-FLAG-Ku80 ((Britton et al. ...
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bioRxiv - Cancer Biology 2021Quote: ... The transfer vector was then packaged into a lentivirus using the pPACKH1 HIV third generation lentiviral expression system kit and PureFection reagent (both from System Biosciences, Palo Alto, CA) into HEK293-T cells (ATCC ...
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bioRxiv - Cell Biology 2021Quote: ... into the XPack CMV-XP-MCS-EF1α-Puro Cloning Lentivector (System Biosciences, Palo Alto, CA). The cyclin D1-APEX plasmids were constructed by combining the PCR fragment of cyclin D1 from the cyclin D1-Flag plasmid and APEX from pcDNA3 APEX-nes (Addgene ...
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bioRxiv - Immunology 2022Quote: ... Constitutive lentiviral expression was performed using pCDH vector constructs (System Biosciences) and packaged using third generation packaging plasmids ...
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bioRxiv - Immunology 2022Quote: ... and inserted into pCDH expression vector (System Biosciences, Mountain View, CA). To generate chDNAM-1-expressing vectors ...
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bioRxiv - Cell Biology 2022Quote: ... and the Super PiggyBac Transposase Expression Vector (System Biosciences, PB210PA-1) using Lipofectamine 3000 Transfection Reagent (ThermoFisher Scientific) ...
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bioRxiv - Neuroscience 2020Quote: ... All plasmids were subcloned into a pCDH expression vector (System Biosciences), assembled using Gibson Assembly (New England Biolabs ...
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bioRxiv - Immunology 2023Quote: ... Constitutive lentiviral expression was performed using pCDH vector constructs (System Biosciences) and packaged using third-generation packaging plasmids ...
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bioRxiv - Immunology 2023Quote: ... Constitutive lentiviral expression was performed using pCDH vector constructs (System Biosciences) and packaged using third-generation packaging plasmids ...
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bioRxiv - Cell Biology 2022Quote: ... These constructs are based on an empty parent minicircle cloning vector (MN100A-1; System Biosciences (SBI), Palo Alto ...
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bioRxiv - Genomics 2022Quote: ... and the Super piggyBac Transposase expression vector (SBI System Biosciences, PB200PA-1,) using the Lipofectamine™ LTX Reagent with PLUS™ Reagent (ThermoFisher Scientific ...
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bioRxiv - Molecular Biology 2021Quote: Super PiggyBac™ Transposase Expression Vector was purchased from System Biosciences (PB210PA-1) and added at a ratio of 5 ng/μL of transfection mix ...
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bioRxiv - Neuroscience 2021Quote: ... PB-EF1α-GreenPuro-H1MCS and Super PiggyBac transposase expression vector from System Biosciences (Cat#PBS506A-1 and Cat# PB210PA-1 respectively ...
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bioRxiv - Molecular Biology 2021Quote: ... Next day they were transfected with HyPB7 and PiggyBac expression plasmids (System biosciences) using lipofectamine 3000 (Thermo Fisher ...
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bioRxiv - Cancer Biology 2023Quote: ... we used the PCDF1-MCS2-EF1-Puro expression vector (System Biosciences, CD110B-1). We performed a restriction digest and gel extraction as above to the plasmid ...
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bioRxiv - Developmental Biology 2023Quote: ... pPN4586× -MT2_Mm was co-transfected with Super piggyBac Transposase expression vector (System Biosciences) into an ESC line with Dox-inducible dCas9 ...
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bioRxiv - Cell Biology 2024Quote: ... The sequence was inserted into lentiviral expression vector pCDH533-IRES-Neo (System Biosciences) to become pCDH533-HA-hK6a-Flag-IRES-Neo ...
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bioRxiv - Molecular Biology 2021Quote: ... This plasmid was co-transfected with piggyBac Transposase expression vector (System Biosciences, PB210PA-1) into the PABPC1-AID cell line (sC152-C16) ...
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bioRxiv - Genetics 2019Quote: ... We transfected the plasmids with Super PiggyBac Transposase Expression Vector (System Biosciences, PB210PA-1) using Lipofectamine™ 3000 Transfection Reagent ...
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Parallelized engineering of mutational models using piggyBac transposon delivery of CRISPR librariesbioRxiv - Bioengineering 2020Quote: ... we developed the excision-only PB transposase plasmid pXPBase-GFP through multiple cloning steps modifying PB220PA-1 (System Biosciences). The ccdB gene was removed to render the plasmid generally propagable ...
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bioRxiv - Cell Biology 2021Quote: ... The cDNAs were then cloned into the peGFP-N1 backbone by Cold Fusion Cloning (System Biosciences, Cat# MC010B-1) following the manufacturer’s instruction ...
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bioRxiv - Biochemistry 2021Quote: ... Plasmids for stable cell line construction were generated by Gibson cloning into pCDH-EF1-CymR-T2A-Neo (System Bioscience). Sequences of all plasmids were confirmed by Sanger sequencing.
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bioRxiv - Bioengineering 2023Quote: ... Plasmids for stable cell line construction were generated by Gibson cloning into pCDH-EF1-CymR-T2A-Neo (System Bioscience) vector ...
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bioRxiv - Cell Biology 2023Quote: ... 1 million cells were co-transfected with 1 µg ePiggyBac transposase expression plasmid (System Biosciences) and 1 µg each epB-MCP-mScarletI ...
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bioRxiv - Immunology 2024Quote: ... The PCR fragments were cloned into a PiggyBac mammalian expression vector (System Biosciences, PB210PA-1), modified to harbor a Secrecon-AA signal peptide111 for secretion at the N-terminus ...
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bioRxiv - Cell Biology 2023Quote: Plasmids with expression cassettes flanked by transposable elements were co-transfected with piggyBac transposase (System Biosciences) into clone 5E6L Caco-2BBe cells ...