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Citations for System Biosciences :
1 - 50 of 246 citations for TIM 1 Mouse HEK 293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... with the respective lamin containing or empty (Mock) vectors into HEK 293-TN cells using PureFection nanotechnology-based transfection reagent (System Biosciences). The medium was exchanged the next day and replaced by antibiotics-free DMEM containing 10% FCS ...
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bioRxiv - Bioengineering 2020Quote: ... these HEKs were genetically-modified reporter cells (NF-κB/293/GFP-Luc™ Transcriptional Reporter Cell Line; System Biosciences, catalogue number TR860A-I), but reporter activity is not relevant here.
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bioRxiv - Immunology 2021Quote: ... HEK-293TN (System Bioscience) cells were plated in 15-cm dish complete DMEM medium ...
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bioRxiv - Cell Biology 2021Quote: ... 293-TN cells (System Biosciences) were co-transfected with lentiviral packaging plasmid and envelope plasmid (psPAX2 and pMD2.G ...
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bioRxiv - Cell Biology 2022Quote: ... 293-TN cells (System Biosciences, SBI) were co-transfected with the lentiviral plasmid and lentiviral helper plasmids (psPAX2 and pMD2.G ...
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bioRxiv - Cancer Biology 2022Quote: ... 293-TN cells (System Biosciences, SBI) at 70-80% confluency were co-transfected with the lentiviral plasmid and lentiviral helper plasmids (psPAX2 ...
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bioRxiv - Cell Biology 2019Quote: ... 293-TN cells (System Biosciences, SBI) were co-transfected with the lentiviral-containing ...
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bioRxiv - Cell Biology 2023Quote: ... and HEK 293TN (LV900A-1) cells were purchased from System Biosciences (Palo Alto, USA). Mouse mesothelial cells (MCs ...
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bioRxiv - Cancer Biology 2023Quote: ... Lentiviruses containing the luciferase expression cassette were produced in 293 TN cells (System Bioscience, catalog number: LV900A-1) by transient transfection with the PSMPUW-Hygro lentiviral vector carrying the luciferase gene and the ViraSafe™ Lentiviral Packaging System (Cell Biolabs ...
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bioRxiv - Molecular Biology 2019Quote: HeLa cervix carcinoma cells (American Tissue Culture Collection: Manassas, VA, USA) and human embryonic kidney (HEK) 293TN cells (System Biosciences, LV900A-1) were cultured using Dulbecco’ s Modified Eagle Medium (DMEM ...
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bioRxiv - Immunology 2022Quote: ... encoding HLA-A*02:01 or HLA-A*03:01 by spinoculation with ultracentrifuge concentrated viral particles produced from 293T-HEK-TN cells (System biosciences), followed by sorting of cells stained with anti-human HLA-A ...
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bioRxiv - Bioengineering 2023Quote: ... were purchased from the Riken Brain Science Institute (Japan) and used to generate lentivirus particles by co-transfecting HEK 293TN cells (System Biosciences) with packaging (psPAX2 ...
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bioRxiv - Microbiology 2019Quote: Mouse G3BP1 cDNAs were subcloned into pCDH-MCS-T2A-puro-MSCV lentiviral vector (System Biosciences) by NEBuilder HiFi DNA assembly (New England Biolabs) ...
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bioRxiv - Cancer Biology 2023Quote: Mouse SIX4 cDNA (Genomics-online) was cloned into pCDH-CMV-MCS-EF1α-Puro Lentivector (System Biosciences). Human SIX4 cDNA was amplified from genomic DNA by PCR and cloned into pCDH-CMV-MCS-EF1α-Puro vector.
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bioRxiv - Molecular Biology 2021Quote: ... with 1/5 final dilution (System Biosciences, LV810A-1), and the mixture was incubated at 4 °C for 6 hrs ...
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bioRxiv - Molecular Biology 2019Quote: ... with 1 μg of transposase plasmid (System Biosciences #PB210PA-1) under conditions described above ...
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bioRxiv - Microbiology 2020Quote: EV isolation from mouse serum was performed using ExoQuick™ solution (System Biosciences, Palo Alto, CA, USA). Following serum collection ...
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bioRxiv - Cell Biology 2022Quote: ... a lentivirus expression plasmid (MMIR-1a-1-PA-1 and MMIR-206-PA-1 purchased from System Biosciences) and plasmid mixture for packaging (ViraPower HiPerform Lentiviral Expression Systems ...
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bioRxiv - Biochemistry 2020Quote: ... anti-TSG101 antibody (EXOAB-TSG101-1; System Biosciences, CA; 1:500), anti-CD63 antibody (EXOAB-CD63A-1 ...
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bioRxiv - Biochemistry 2020Quote: ... anti-CD63 antibody (EXOAB-CD63A-1; System Biosciences, CA; 1:500), anti-CD5L antibody (AF2834 ...
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bioRxiv - Cell Biology 2021Quote: ... AP100R-1 (System Biosciences) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... Lentivector-based scrambled miRZip-000 (MZIP000-PA-1) and miRZip-146a (anti-miRNA; MZIP146a-PA-1) in S1505A-1 construct vector were purchased from System Biosciences. Both Scrambled and miRZIP vectors contained copGFP as a fluorescent reporter ...
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bioRxiv - Cancer Biology 2022Quote: ... Both human and mouse BGN were subcloned into the PB-CMV-MCS-EF1α-Puro piggybac expression vector (System Biosciences) using the 5’ EcoRI and 3’ BamHI restriction sites ...
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bioRxiv - Cancer Biology 2024Quote: cDNA of mouse Dnmt3A1WT and DnmtA1W326R (corresponding to human W330R) previously cloned into pCDH-EF1-MCS-Neo (System Biosciences) with an N-terminal FLAG-HA epitope tag(37 ...
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bioRxiv - Cancer Biology 2023Quote: ... along with two housekeeping genes (Supplementary Table 1; CAS9-PR-1 from System Biosciences). The Kcnn4 and Cas9 mRNA levels for each sample were measured in technical duplicates for each primer set ...
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bioRxiv - Developmental Biology 2020Quote: Human SC102A-1 (System Bioscience) and chimpanzee Sandra A iPSC lines (Camp et al. ...
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bioRxiv - Microbiology 2022Quote: HEK293T (System Biosciences, LV900A-1) and HEK293-Spike cells (SARS-CoV-2 Spike (D614)-expressing 293 cells [293-SARS2-S cells ...
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bioRxiv - Developmental Biology 2020Quote: ... DLD-1 cells were co-transfected with a PiggyBac transposase vector (System Biosciences, PB210PA-1) and a PiggyBac-ABA-CRISPRi vector (25 ...
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bioRxiv - Cell Biology 2023Quote: ... 1 million cells were co-transfected with 1 µg ePiggyBac transposase expression plasmid (System Biosciences) and 1 µg each epB-MCP-mScarletI ...
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bioRxiv - Developmental Biology 2023Quote: ... or goat anti-rabbit (1:20,000; EXOAB-KIT-1; System Biosciences, Palo Alto, CA, USA). Protein signals were detected using the SuperSignal West Femto Chemiluminescent kit (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... and incubated overnight at 4°C with 1:1000 primary antibody (CD63 and CD81, rabbit anti-human, EXOAB-CD63A-1/ EXOAB-CD81A-1, System Biosciences, Palo Alto, CA, USA) in TBST ...
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bioRxiv - Molecular Biology 2019Quote: HEK293TN cells (System Biosciences, LV900A-1) and HeLA cells (American Tissue Culture Collection ...
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bioRxiv - Molecular Biology 2019Quote: HEK293TN cells (System Biosciences, LV900A-1) were cultured using Dulbecco’s Modified Eagle Medium (DMEM ...
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bioRxiv - Genomics 2022Quote: THP-1 cells were transduced in triplicates using the TransDux MAX transfection reagent (System Biosciences, LV860A-1) according to the manufacturer’s recommendations ...
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bioRxiv - Genetics 2021Quote: ... along with 1/10 total plasmid mass of Super PiggyBac Transposase Expression Vector (System Bioscience, Inc. #PB210PA-1). Stable integrants were selected with 1-2 μg/mL puromycin.
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bioRxiv - Cell Biology 2022Quote: ... Stock cumate solution (System Biosciences # QM100A-1) was diluted to 5X in culture medium prior to use.
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bioRxiv - Cell Biology 2021Quote: ... with piggyBac transposase (PB210PA-1, System Biosciences) and a piggyBac plasmid expressing SpCas9 under a doxycycline inducible promoter and containing a blasticidin selection cassette ...
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bioRxiv - Immunology 2021Quote: IL-23 EEV(EEV651A-1, System Biosciences) was hydrodynamically injected in a volume of sterile HBSS (Invitrogen ...
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bioRxiv - Cell Biology 2023Quote: ... and HEK293TN cells (System Biosciences, #LV900A-1) were routinely cultured in Dulbecco’s Modified Eagle Medium (DMEM ...
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bioRxiv - Molecular Biology 2023Quote: ... ni piggyBac transposase (PB200PA-1; System Biosciences) (2) ...
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bioRxiv - Microbiology 2023Quote: ... and HR110PA-1 (System Biosciences, CA, USA). The pX330-puro vector was constructed by integrating the puromycin resistance gene sequence from the HR110PA-1 vector into the pX330 vector ...
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bioRxiv - Cancer Biology 2024Quote: ... and 0.5% TransDux (LV860A-1, System Biosciences). 72 hours after transduction ...
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bioRxiv - Neuroscience 2020Quote: ... and the membrane and intra-vesicular positive EV markers CD81 (1:500; cat. no. EXOAB-CD81A-1, System Biosciences) and alix (0.4 μg/mL ...
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bioRxiv - Cell Biology 2020Quote: ... or a pCDH vector (System Biosciences, CD550A-1). JNK1 (NCBI RefSeq NM_001323302.1 ...
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bioRxiv - Neuroscience 2022Quote: ... HEK293TN cells (System Biosciences, cat. no. LV900A-1) and HeLa cells (Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures GmbH ...
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bioRxiv - Bioengineering 2021Quote: ... coli Minicircle Strain (System Biosciences, catalog #: MN900A-1) overnight at 37°C ...
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bioRxiv - Cancer Biology 2022Quote: ... pGreenpuro shRNA cloning and expression lentivector was purchased from System Bioscience (Cat. No. SI505A-1, System Biosciences, LLC, CA). Primers for shRNA are shown in S1 Table ...
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bioRxiv - Developmental Biology 2022Quote: ... using Cold Fusion cloning (System Biosciences, MC010B-1). All constructs were sequence verified for successful insert integration ...
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bioRxiv - Genetics 2022Quote: ... and concentrated using PEG (System Biosciences, LV825A-1) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... dual-reporter lentiviral plasmids pLL410_EF1a-rFLuc-T2A-GFP-mPGK-Puro (LL410PA-1) and pLL-CMV-rFLuc-T2A-GFP-mPGK-Puro (LL310PA-1) were obtained from System Bioscience. Lentiviral generation was carried out as previously described [11] ...