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Citations for System Biosciences :
1 - 50 of 115 citations for Human KIRREL shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: shRNAs targeting human HNRNPR were cloned into a modified version of pSIH-H1 shRNA vector (System Biosciences) containing EGFP according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: shRNAs targeting human HNRNPR were cloned into a modified version of pSIH-H1 shRNA vector (System Biosciences) containing EGFP according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... two different short hairpin RNAs (shRNAs) and a scramble shRNA were cloned into pSIH1-H1-copGFP shRNA lentiviral vector (System Biosciences #SI501A-A). Generation of lentiviruses and further specifications are described in supplemental methods.
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bioRxiv - Neuroscience 2021Quote: ... shRNAs targeting mouse drebrin A and shRNAs targeting both drebrin A and E were cloned into a modified version of pSIH-H1 shRNA vector (System Biosciences) containing eGFP according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2022Quote: ... shRNA templates were cloned into pGreenPuro lentivector (System Biosciences, SI505A-1). shRNA targets are ...
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bioRxiv - Cancer Biology 2022Quote: ... pGreenpuro shRNA cloning and expression lentivector was purchased from System Bioscience (Cat ...
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bioRxiv - Molecular Biology 2023Quote: ... were seeded and transfected with pSIH-H1 shRNA lentivectors (System Biosciences) and packaging mix (Invitrogen ...
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bioRxiv - Systems Biology 2020Quote: ... GFP gene was amplified from pGreenPuro shRNA Cloning and Expression Lentivector (CMV; System biosciences, SI505A-1) and sub-cloned into ITR-CAG-DEST-IRES-Neomycin-ITR plasmid (generous gift from Marc Supprian Schmidt) ...
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bioRxiv - Neuroscience 2019Quote: ... a published Snapin KD sequence (64) was cloned into the pSIH-H1 shRNA vector (System biosciences). Inserted sequences are described in Suppl ...
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bioRxiv - Cancer Biology 2021Quote: ... The lentiviral plasmid expressing SOX2 was constructed by cloning human SOX2 ORF cDNA into the pCDH-CMV-MCS-EF1a-Puro vector (System Biosciences, CD510B-1) or PCW57.1 vector (Addgene ...
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bioRxiv - Cell Biology 2020Quote: ... complementary primers were hybridized and cloned into the BamHI and EcoRI sites of the lentiviral pSIH-H1-copGFP-shRNA vector (System Biosciences) according to the manufacturer’s instructions to generate pSIH-T1 and pSIH-T2 plamsids for constitutive knockdown ...
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bioRxiv - Developmental Biology 2020Quote: Human SC102A-1 (System Bioscience) and chimpanzee Sandra A iPSC lines (Camp et al. ...
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bioRxiv - Cancer Biology 2021Quote: ... the envelope plasmid (pMD2.G) and the packaging plasmid (pCMV-deltaR8.91) (System Biosciences, CA, USA) into HEK293T cells according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... the SpyCas9-ABE-tracrRNA PiggyBac transposon plasmid and PiggyBac transposase expression plasmid (System Biosciences PB210PA-1) were mixed in a 2:1 molar ratio and transfected into the HEK293T-dGFP reporter cell line using Lipofectamine 3000 (ThermoFisher L3000015) ...
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bioRxiv - Molecular Biology 2024Quote: ... Lentiviruses were generated by transfecting the plasmids using pPACKH1 Packaging Plasmid Mix (LV500A-1; System Biosciences) into a 293T cell line (LV900A-1 ...
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bioRxiv - Cancer Biology 2020Quote: ... UCHL1 knockdown was also carried out by transducing HGSOC cells with control or UCHL1 shRNA lentiviral virus particles (Santacruz Biotechnology; cat# sc-108080 and sc-42304-V) using TransDux™ MAX (System Biosciences; cat#LV860A-1). Cells were treated with Carfilzomib (cat# S2853 ...
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bioRxiv - Cancer Biology 2023Quote: ... Luc-plasmid was packaged with pPACKH1 plasmid mix using the protocol described by the manufacturer (System Biosciences Inc.). Cell lines were transduced with Luc-plasmid using Sigma® MISSION® ExpressMag® Beads (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2023Quote: ... 2000 ng of the PiggyBac vector plasmid and 500 ng of the PiggyBac transposase plasmid (System Biosciences, PB210PA-1) were mixed in 125 μL Opti-MEM (Gibco ...
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bioRxiv - Cell Biology 2020Quote: ... along with PiggyBac Transposase expression plasmid (System Biosciences) and selected with 500 μg/ml G418 (Invitrogen) ...
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bioRxiv - Developmental Biology 2023Quote: ... and 420 ng PBase ‘helper’ plasmid (System Biosciences). After preparation ...
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bioRxiv - Cell Biology 2021Quote: ... the piggyBac transposon plasmid that contains the desired transgene and a piggyBac transposase plasmid (PB210PA-1, System Biosciences, Palo Alto, CA) were electroporated into TP53-/- RPE-1 cells simultaneously using the Neon electroporation system according to the manufacturer’s instruction (Invitrogen) ...
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bioRxiv - Microbiology 2020Quote: The 3’UTR reporter plasmids were co-transfected into HEK293T cells using PEI max together with the indicated pCDH-EF1-MCS expressing plasmids (System Biosciences) containing a cloned pre-miRNA gene of interest ...
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bioRxiv - Developmental Biology 2019Quote: ... the PR-deletion coding sequences or the NLS-3xFlag was digested from their pCAGIG plasmids and inserted sequentially to the pCDH-CMV-MCS-EF1-Puro plasmid (System Biosciences) between the EcoRI and NotI sites (for Prdm16-FL and Prdm16-PRdeletion ...
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bioRxiv - Cancer Biology 2023Quote: ... The pSIH1-H1-siLuc-copGFP plasmid (System Bioscience, LV601PB) was used as a positive transduction control.
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bioRxiv - Neuroscience 2024Quote: pCDH-EF1-MCS-IRES-copGFP lentiviral plasmid (System Biosciences) was used as the backbone to create (G4C2)92 and (G4C2)2 lentiviral constructs ...
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bioRxiv - Immunology 2023Quote: ... or luciferase used the same packaging and envelope plasmids as described previously with the respective 3rd generation plasmids: pCDH-MSCV-MCS-EF1-GFP-T2A-Puro (System Biosciences, CD713B-1), pLenti-mCherry (derived from pLenti-III-EF1a ...
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bioRxiv - Synthetic Biology 2021Quote: ... A total of 4 μg of piggyBac targeting base editor plasmids were transfected with 1 μg of super transposase plasmid (SBI System Biosciences cat# PB210PA-1) using Lipofectamine 3000 following manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2022Quote: ... Plasmids encoding different genetic payloads (transfer plasmids) were designed in SnapGene software (GSL Biotech LLC) and sub-cloned into lentivirus vector plasmid (System Biosciences, Cat #CD510B-1) or PiggyBac Transposon vector plasmid (System Biosciences ...
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bioRxiv - Genetics 2021Quote: ... This plasmid was derived from the MN530A-1 (System Biosciences) by replacing the GFP Reporter sequence with a multiple cloning site using the restriction enzymes XmaI and StuI and duplexed donor oligonucleotides #2020 and #2021 (Supplemental Table 1) ...
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bioRxiv - Molecular Biology 2019Quote: ... with 1 μg of transposase plasmid (System Biosciences #PB210PA-1) under conditions described above ...
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bioRxiv - Molecular Biology 2020Quote: The pCDH-EF1-Puro plasmid was obtained from System Biosciences. The pCDH-DJ-1 WT and mutants were constructed using the primers listed in table S2 ...
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bioRxiv - Synthetic Biology 2022Quote: ... or PiggyBac Transposon vector plasmid (System Biosciences, Cat # PB510B-1). Plasmids encoding 2nd-generation packaging plasmids (psPAX2 – Cat #12260 ...
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bioRxiv - Synthetic Biology 2022Quote: ... and mTagBFP2 into a lentiviral backbone plasmid (System Biosciences # TR012PA).
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bioRxiv - Neuroscience 2022Quote: ... The Super PiggyBac Transposase plasmid (System Biosciences; Cat# PB210PA-1) was obtained from BioCat ...
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bioRxiv - Developmental Biology 2023Quote: All constructs were cloned into a PiggyBac plasmid (System Biosciences). Linear DNA fragments were amplified via PCR using CloneAmp HiFi PCR premix (Takara Bio ...
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bioRxiv - Cell Biology 2023Quote: ... 1.8 µg PB533-or PB510-based PiggyBac plasmid (System Biosciences) and 0.2 µg transposase expression vector (System Biosciences ...
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bioRxiv - Neuroscience 2024Quote: LDLR and spaghetti monster plasmids were designed by System Biosciences. The AAV-BR1 packaging vector was a gift from Jacob Korbelin ...
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bioRxiv - Synthetic Biology 2020Quote: ... The human proteins were assigned to 3 Piggybac vectors (System Biosciences, USA) by Golden Gate Assembly method (11 ...
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bioRxiv - Synthetic Biology 2020Quote: ... All constructs were cloned into the piggyBac plasmids (System Biosciences Inc.) driven by a synthetic version of human EF1A promoter.
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bioRxiv - Neuroscience 2020Quote: ... All plasmids were subcloned into a pCDH expression vector (System Biosciences), assembled using Gibson Assembly (New England Biolabs ...
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bioRxiv - Microbiology 2020Quote: ... for 2 h at 37°C and plasmid-safe DNase (System Bioscience) overnight at 37°C ...
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bioRxiv - Cell Biology 2020Quote: ... into the third-generation lentiviral plasmid pCDH-EF1-IRES-Puro (System Biosciences).
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bioRxiv - Synthetic Biology 2023Quote: ... HEK293FT cells were transfected with the vector and transposon plasmid (System Biosciences). Transfected Cells were incubated for 72 hours before selection with media containing 2 ug/mL Puromycin (Invivogen ant-pr-1) ...
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bioRxiv - Cell Biology 2023Quote: The let-7 overexpression plasmids are commercially available from System Biosciences (SBI). The catalog numbers of the plasmids used in this research are PMIRH000PA-1 (empty vector) ...
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bioRxiv - Biophysics 2020Quote: ... Piggybac plasmid PB513Bm2 was made by removing copGFP from PB513B-1 (System Biosciences) by PCR-based mutagenesis ...
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bioRxiv - Cell Biology 2019Quote: Lentiviral transfer plasmids were constructed from pCDH-EF1-MCS-IRES-Puro (System Bioscience). Briefly ...
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bioRxiv - Molecular Biology 2020Quote: ... miR-Zip control and miR-Zip-200c plasmids were purchased from System Biosciences.
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bioRxiv - Molecular Biology 2021Quote: ... Next day they were transfected with HyPB7 and PiggyBac expression plasmids (System biosciences) using lipofectamine 3000 (Thermo Fisher ...
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bioRxiv - Cell Biology 2023Quote: ... The plasmid vector pCDH-EF1-MCS-T2A-copGFP was purchased from System Biosciences. The lentiviral vector was packaged in HEK293T cells as previously described ...
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bioRxiv - Cancer Biology 2023Quote: ... plasmid pCDH-CMV-MCS2-EF1-Hygro (System Biosciences Inc., Palo Alto, CA, USA) cloned with Luc2 open reading frame (ORF ...