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Citations for System Biosciences :
1 - 50 of 84 citations for Chikungunya Virus E2 Envelope Protein HEK293 Human Fc Tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... and virus was concentrated with PEG-it Virus Precipitation solution (System Biosciences). SORE6-GFP virus was added to CSCs plated on Geltrex ...
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bioRxiv - Cancer Biology 2019Quote: ... the virus media was concentrated using PEG-it Virus Precipitation Solution (SBI System Biosciences), and then the lentiviral particles resuspended in 300 µL PBS ...
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bioRxiv - Cancer Biology 2022Quote: ... Media containing virus particles was mixed with PEG-it virus precipitation solution (System Biosciences, #LV810A-1) and incubated at 4°C overnight ...
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bioRxiv - Immunology 2019Quote: ... Virus particles were collected and concentrated by precipitation with PEG-it Virus Precipitation Solution (5x) (System Biosciences) and used to transduce pre-activated primary human CD4+ T cells in combination with Transdux (System Biosciences) ...
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bioRxiv - Synthetic Biology 2020Quote: ... The human proteins were assigned to 3 Piggybac vectors (System Biosciences, USA) by Golden Gate Assembly method (11 ...
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bioRxiv - Cell Biology 2022Quote: ... IL) lentiviruses were grown in human embryonic kidney 293T cells and concentrated using the PEG-it Virus Precipitation Solution (System Biosciences, Mountain View, CA), as previously described (51) ...
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bioRxiv - Genomics 2023Quote: ... The collected virus was concentrated 100-fold after precipitation with PEG-itTM Virus Precipitation Solution (System BioSciences LV810A-1) for 3 days ...
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bioRxiv - Cancer Biology 2021Quote: ... the envelope plasmid (pMD2.G) and the packaging plasmid (pCMV-deltaR8.91) (System Biosciences, CA, USA) into HEK293T cells according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... Culture medium containing the shed virus was collected at 72 hours and incubated with PEG-it virus precipitation solution (System Biosciences) at 4°C for 48 hours ...
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bioRxiv - Cancer Biology 2023Quote: ... MCF10A cells were seeded to achieve 50% to 70% confluency and the following day the virus particles were added to the dish along with TransDux virus transduction reagent (System Biosciences, LV850A). The media was changed after 4 days and antibiotic selection was performed using a puromycin concentration of 0.85µg/mL ...
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bioRxiv - Cancer Biology 2023Quote: ... Virus was concentrated with PEG-it solution (System Biosciences) according to the manufacturer’s instructions.
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Hydrogen sulfide blocks HIV rebound by maintaining mitochondrial bioenergetics and redox homeostasisbioRxiv - Microbiology 2021Quote: ... Virus was concentrated using 5× PEG- it™ (System Biosciences) as per manufacturer’s protocol and virus pellet obtained was aliquoted in opti-MEM and stored at -80°C ...
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bioRxiv - Molecular Biology 2021Quote: ... filtered and concentrated using PEG-it Virus Precipitation Solution (System Biosciences) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... 5x PEG-it Virus Precipitation Solution (System Biosciences, Cat # LV810A-1) was added to supernatants and incubated 4°C overnight ...
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bioRxiv - Molecular Biology 2020Quote: ... filtered and concentrated using PEG-it Virus Precipitation Solution (System Biosciences) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... and concentrated using the PEG-it Virus Precipitation Solution (System Biosciences) according to the manufacturer’s directions ...
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bioRxiv - Molecular Biology 2022Quote: ... The lentivirus was concentrated by PEG-it Virus Precipitation Solution (System Biosciences) and stored in –80°C freezer.
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bioRxiv - Molecular Biology 2021Quote: ... The viruses were then concentrated using PEG-it Virus Precipitation Solution (System Biosciences) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... Virus was concentrated by adding 2500 μl ice-cold PEG-IT (System Biosciences) to 10 ml harvested supernatants and incubating overnight at 4°C ...
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bioRxiv - Genomics 2021Quote: ... Filtered supernatant was concentrated using the PEG-it Virus Precipitation Solution (System Biosciences) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... The virus was either used directly or concentrated using PEG-it (System Biosciences) before use ...
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bioRxiv - Cell Biology 2023Quote: ... 0.25ml of cold PEG-it Virus Precipitation Solution (LV810A-1-SBI, System Biosciences) was added per 1ml of lentiviral-containing supernatant and kept cool at 4°C overnight ...
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bioRxiv - Immunology 2023Quote: ... or luciferase used the same packaging and envelope plasmids as described previously with the respective 3rd generation plasmids: pCDH-MSCV-MCS-EF1-GFP-T2A-Puro (System Biosciences, CD713B-1), pLenti-mCherry (derived from pLenti-III-EF1a ...
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bioRxiv - Bioengineering 2020Quote: ... viral supernatant was concentrated using PEG-it virus precipitation solution (System Biosciences LV825A-1) according to manufacturer instructions ...
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bioRxiv - Neuroscience 2020Quote: ... Harvested lentivirus was concentrated using PEG-it (™) virus precipitation solution (System Biosciences, CA) and processed for titration.
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bioRxiv - Bioengineering 2019Quote: ... Viral supernatant was concentrated using PEG-it Virus Precipitation Solution (System Biosciences, Palo Alto, CA) following the manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2020Quote: ... and then transfected with three piggyBac vectors expressing multiple human proteins with the Neon Transfection System with the presence of piggyBac Transposase (System Biosciences, USA). Two days after ...
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bioRxiv - Immunology 2021Quote: ... A PEG-it Virus Precipitation Solution (Cat# LV810A-1, System Biosciences, Palo Alto, CA 94303, USA) was added to 40ml of virus culture at a 1:4 ratio ...
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bioRxiv - Cancer Biology 2024Quote: ... stable HEK293-F cell lines for each cytokine were prepared through cloning into the expression cassette of PiggyBac (System Biosciences) transposon vector ...
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bioRxiv - Neuroscience 2022Quote: ... and after three days harvested and concentrated the Virus with the AAVanced™ Concentration Reagent (System Biosciences).
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bioRxiv - Genomics 2020Quote: ... multiple viral supernatants were collected and concentrated using PEG-it Virus Precipitation Solution (System Biosciences LV810A-1). Cells were infected 2-3 times at 12-hour intervals before selection in the appropriate antibiotic.
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bioRxiv - Cell Biology 2020Quote: ... collected after 24 hours and concentrated using Peg-It Virus precipitation solution (cat# LV810A-1, System Biosciences) according to the manufacturer’s manual.
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bioRxiv - Molecular Biology 2023Quote: ... filtered using 45 um filters and concentrated 100-fold using PEG-it Virus Precipitation Solution (System Biosciences).
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bioRxiv - Developmental Biology 2020Quote: Human SC102A-1 (System Bioscience) and chimpanzee Sandra A iPSC lines (Camp et al. ...
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bioRxiv - Cell Biology 2020Quote: Lentivirus was generated with PEG-it Virus Precipitation Solution (System Biosciences, Palo Alto, CA, USA no LV810A-1) using a LV H2B-GFP plasmid (Addgene no 25999 ...
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bioRxiv - Cell Biology 2024Quote: ... Viral transduction of matured iPS-RPE were aided using TransDux virus transduction reagent (System Biosciences, cat# LV850A-1) and spin infection (800 g ...
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bioRxiv - Molecular Biology 2021Quote: ... The WPRE (Woodchuck Hepatitis Virus Posttranscriptional Regulatory Element) 3’-UTR sequence was amplified from the CD813A-1 (System Biosciences) vector.
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bioRxiv - Cancer Biology 2021Quote: ... This supernatant containing lentiviral particles was concentrated using PEGit virus precipitation solution according to the manufacturer’s protocol (System Biosciences). JAM-A knockdown constructs were as follows ...
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bioRxiv - Cancer Biology 2022Quote: ... supernatant containing lentiviral particles was collected and concentrated with PEGit virus precipitation solution according to manufacturer’s protocol (System Biosciences). CSCs were plated on Geltrex ...
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bioRxiv - Molecular Biology 2023Quote: ... and the viral supernatants were passed through 0.45-μm filters and concentrated using PEGit Virus Precipitation Solution (System Biosciences). The lentiviral vectors were resuspended in Opti-MEM (GIBCO ...
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bioRxiv - Cancer Biology 2023Quote: ... The viruses from each time point were pooled and concentrated using PEG-it virus precipitation solution (System Biosciences, LV810A) according to manufacture’s protocol ...
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bioRxiv - Cell Biology 2020Quote: ... EV depleted FCS was either commercially obtained (System Biosciences Catalog no. EXO-FBS-250A-1) or prepared by ultracentrifugation of the FCS used at 120,000 x g for 5 hours ...
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bioRxiv - Biophysics 2022Quote: ... Lentiviral libraries were prepared as described above in complete DMEM and harvested using PEG-it Virus Precipitation Solution (System Biosciences). Lentiviral titers were checked using the hFzd5CRD-1TM cells at 2 ml scale based on Wnt expression ...
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bioRxiv - Neuroscience 2022Quote: ... The lentivirus was concentrated with the use of the PEG-it virus precipitation solution (2.5 ml, System Biosciences, LV810A-1) for 2-3 days at 4°C ...
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bioRxiv - Biochemistry 2023Quote: The PiggyBac-cumate-ERGIC-53-PA tag plasmid along with the Super PiggyBac Transposase Expression Vector (System Bioscience) was transfected to generate a stable HEK293T cell line with cumate inducible ERGIC-53 expression ...
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bioRxiv - Neuroscience 2021Quote: ... Lentiviral particles were harvested either by ultracentrifugation at 106,559 g for 2 h at 4°C using a Beckman SW 32 Ti rotor or using the PEG-it Virus Precipitation Solution (System Biosciences) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... Medium containing lentiviral particles was collected at 48 and 72 hours post transfection and concentrated using PEG-it Virus Precipitation Solution (System BioSciences). Precipitated lentiviral particles were resuspended in DMEM ...
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bioRxiv - Immunology 2022Quote: ... VLPs were precipitated from the clarified supernatant by the addition of PEG-it Virus Precipitation Solution (System Biosciences, Palo Alto, CA, USA) at a ratio of 1:10 ...
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bioRxiv - Cancer Biology 2022Quote: The infection of HuCCT1 cells with pGreenFire 2.0 TCF/LEF reporter virus (pGF2-TCF/LEF-rFluc-T2A-GFP-mPGK-Puro) (SBI System Biosciences, TR413VA-P) was performed using SureENTRY Transduction Reagent ...
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bioRxiv - Molecular Biology 2024Quote: ... The pooled supernatant was then filtered with 0.45 μm membrane and concentrated using the PEG-it virus precipitation solution (System Biosciences #LV810A-1) according to the manufacturer’s instructions ...