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Citations for System Biosciences :
1 - 50 of 95 citations for Adenovirus Type 5 Particles CMV β galatosidase since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... into XPack CMV constructs (System Biosciences). The cyclin D1-GFP plasmids were constructed by combining the PCR fragment of cyclin D1 from the cyclin D1-Flag plasmid and GFP from pEGFP-N1 (BD Biosciences Clontech ...
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bioRxiv - Cell Biology 2021Quote: ... into XPack CMV constructs (System Biosciences). For cyclin D1 overexpression ...
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bioRxiv - Biochemistry 2021Quote: ... pCDH-CMV-MCS-EF1-Puro (System Biosciences) for the viral infection and expression of N-terminal FLAG-tagged USP8 ...
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bioRxiv - Immunology 2021Quote: ... pCDH-CMV-MCS-EF1α-RFP (System Biosciences). To facilitate the secretion and purification of the protein ...
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bioRxiv - Cell Biology 2022Quote: ... The pCDH-CMV-MCS-EF1-Blast was generated from pCDH-CMV-MCS-EF1-Hygro (plasmid #CD515B-1; System Biosciences). The annealed synthetic oligonucleotides (Table 2 ...
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bioRxiv - Biophysics 2022Quote: ... PB-CMV-MCS-EF1α-Puro (System Bioscience #PB510B-1) was linearized using EcoRI and BamHI ...
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bioRxiv - Cell Biology 2019Quote: ... PiggyBac vector PB-CMV-MCS-EF1a-Puro (System Biosciences) was first modified to include blasticidin resistance gene instead of the puromycin one and linearized using SfiI and XbaI ...
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bioRxiv - Neuroscience 2022Quote: ... digested pCDH-CMV-MSC-T2A-EGFP vector (System Biosciences). mAKT1 (Merck ...
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bioRxiv - Molecular Biology 2023Quote: ... a minimal piggyBac cargo construct was created by deleting all intervening sequences between the 5’ and 3’ terminal repeats (including core insulators) of the PB-CMV-MCS-EF1α-Puro vector (System Biosciences, PB510B-1). A gBlock (Integrated DNA Technologies ...
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bioRxiv - Neuroscience 2023Quote: ... into PB-CMV-MCS-EF1-Puro (System Biosciences #PB510B-1).
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bioRxiv - Neuroscience 2023Quote: ... into PB-CMV-MCS-EF1-Puro (System Biosciences #PB510B-1).
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bioRxiv - Neuroscience 2023Quote: ... into PB-CMV-MCS-EF1-Puro (System Biosciences #PB510B-1).
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bioRxiv - Cell Biology 2022Quote: ... The NTA measurements rely on light scattering to extract particle size and the number of particles in a sample and the NTA software (Version 2.3) collects data on multiple particles to calculate the hydrodynamic diameter of each particle using the Stokes-Einstein equation (System Biosciences, LLC).
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Lentiviral particles were precipitated by PEG-it (System Biosciences) as described by the manufacturer and were resuspended in sterile 1xPBS and frozen in aliquots at −80°C ...
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bioRxiv - Cell Biology 2019Quote: ... was transferred into pCDH-CMV-MCS-EF1-Puro (System Biosciences, cat #CD510B-1) using NheI/NotI sites for virus production.
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bioRxiv - Cancer Biology 2022Quote: We obtained pCDH-CMV-MCS-EF1α-Puro lentivector from System Biosciences (#CD510B-1). To generate the pCDH-NT5C2 expression construct ...
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bioRxiv - Cell Biology 2020Quote: ... was transferred into pCDH-CMV-MCS-EF1-Puro (System Biosciences, cat #CD510B-1) using NheI/NotI sites for virus production.
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bioRxiv - Cancer Biology 2023Quote: ... plasmid pCDH-CMV-MCS2-EF1-Hygro (System Biosciences Inc., Palo Alto, CA, USA) cloned with Luc2 open reading frame (ORF ...
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bioRxiv - Biochemistry 2021Quote: ... Viral particles were concentrated using PEG-it (System Biosciences, Inc).
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bioRxiv - Cell Biology 2024Quote: ... pseudoviral particles were produced using 293TN cells (System Biosciences, SBI). The 293TN cells were transfected with the lentiviral plasmid of interest as well as lentiviral packaging and envelope plasmids (psPAX and pMD2.G ...
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bioRxiv - Microbiology 2021Quote: ... Ceg3 was inserted into pCDH-CMV-MCS-EF1a-RFP (System Biosciences cat# CD512B-1), which was transfected together with pMD2.G (Addgene plasmid #12259 ...
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bioRxiv - Cell Biology 2021Quote: ... into the XPack CMV-XP-MCS-EF1α-Puro Cloning Lentivector (System Biosciences, Palo Alto, CA). The cyclin D1-APEX plasmids were constructed by combining the PCR fragment of cyclin D1 from the cyclin D1-Flag plasmid and APEX from pcDNA3 APEX-nes (Addgene ...
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bioRxiv - Cell Biology 2021Quote: ... the cyclin D1 was generated by PCR insertion into the XPack CMV constructs (System Biosciences). Hsc70D10N was generated by PCR-based site-directed mutagenesis using the QuikChange II XL Site-Directed Mutagenesis Kit (Agilent Technologies) ...
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bioRxiv - Systems Biology 2020Quote: ... GFP gene was amplified from pGreenPuro shRNA Cloning and Expression Lentivector (CMV; System biosciences, SI505A-1) and sub-cloned into ITR-CAG-DEST-IRES-Neomycin-ITR plasmid (generous gift from Marc Supprian Schmidt) ...
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C53 interacting with UFM1-protein ligase 1 regulates microtubule nucleation in response to ER stressbioRxiv - Cell Biology 2020Quote: ... It was thereafter inserted into pCDH-CMV-MCS-EF1-puro vector (System Biosciences, Palo Alto, CA), resulting in vector mNeonGreen-puro ...
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bioRxiv - Cancer Biology 2023Quote: Mouse SIX4 cDNA (Genomics-online) was cloned into pCDH-CMV-MCS-EF1α-Puro Lentivector (System Biosciences). Human SIX4 cDNA was amplified from genomic DNA by PCR and cloned into pCDH-CMV-MCS-EF1α-Puro vector.
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bioRxiv - Cancer Biology 2023Quote: ... A second ESR1-DAB2 plasmid in a pCDH-CMV-MCS-ER1α-Puro (System Biosciences #CD510B-1) was received as a kind gift from Dr ...
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bioRxiv - Immunology 2020Quote: ... Supernatant containing viral particles was collected and concentrated using PEG-IT (System Biosciences) followed by centrifugation at 2600 rpm ...
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bioRxiv - Cancer Biology 2020Quote: ... Viral particles were precipitated from media using PEG-it (System Biosciences, LV810A-1). CP-A-empty and CP-A-ERBB2 stable cells were generated by transfecting 1×106 CP-A cells with lentiviral particles containing either pHAGE-ERBB2 or pHAGE-empty using polybrene (EMD Millipore ...
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bioRxiv - Microbiology 2020Quote: ... cDNA sequences were cloned into the PB-CMV-MCS-EF1α-Puro vector (PB510B-1, SBI System Biosciences) where the CMV promoter was replaced by a PGK promoter ...
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bioRxiv - Microbiology 2021Quote: ... the gfp-lem8 fusion was inserted into pCDH-CMV-MCS-EF1a-Puro (System Biosciences, cat# CD510B-1). The plasmids were co-transfected with pMD2.G (gift from Dr ...
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bioRxiv - Cancer Biology 2022Quote: ... 34kDa isoform of PIM1 in a pCDH-CMV-MCS-EF1α-GreenPuro Cloning and Expression Lentivector(System Biosciences) via the Spinfection method ...
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bioRxiv - Cancer Biology 2021Quote: ... Lentiviral particles were concentrated by precipitation from culture supernatants using PEG-IT (System Biosciences) and quantified with the QuickTiter Lentivirus Titer Kit (Cell Bio Labs).
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bioRxiv - Molecular Biology 2019Quote: ... Pseudoviral particles were prepared using the pPACK-F1 Lentivector packaging system (SBI System Biosciences) and HEK293T producer cell line ...
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bioRxiv - Cancer Biology 2019Quote: ... Lentiviral vectors for GFP-FAK wildtype and FAK kinase-dead (R454) in pCDH-CMV-MCS-Puro (System Biosciences) were used as described (Chen et al. ...
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bioRxiv - Cancer Biology 2019Quote: ... RNS85 and RNS87 were stably transfected at MOI = 0.3 with pCDH-CMV-MCS-EF1-Puro-copGFP (System biosciences) empty vector (EV) ...
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bioRxiv - Cell Biology 2021Quote: The backbone of the original plasmid pCDH-CMV-MCS-EF1-copGFP-T2A-Puro (System Biosciences, Palo Alto, USA) was amplified and linearized via PCR using the high-fidelity PrimeSTAR GXL DNA Polymerase (Takara Bio ...
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bioRxiv - Cancer Biology 2019Quote: Overexpression vectors were constructed using pCDH-CMV-MCS-EF1-GFP cloning/ expression vector (System Biosciences, Palo Alto, CA,USA). C/EBPδ insert was cut out of a PCR4 TOPO cloning vector (Invitrogen ...
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bioRxiv - Cancer Biology 2022Quote: ... Both human and mouse BGN were subcloned into the PB-CMV-MCS-EF1α-Puro piggybac expression vector (System Biosciences) using the 5’ EcoRI and 3’ BamHI restriction sites ...
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bioRxiv - Systems Biology 2023Quote: ... All of the Notch receptor piggyBac constructs were derived from the vector PB-CMV-MCS-EF1-Puro (System Biosciences), with changes made to the promoter (CMV changed to PGK or CAG ...
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bioRxiv - Cell Biology 2020Quote: ... Plasmid was then packaged into viral particles using the pPACKH1 lentivector Packaging Kit (System Bioscience) in HEK293T cells ...
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bioRxiv - Molecular Biology 2024Quote: ... Purified viral particles were transduced into MIN6 cells using TransDux MAX (LV860A-1; System Biosciences) and cultured for 48-72 hours in complete DMEM (11965-092 ...
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bioRxiv - Genetics 2023Quote: The PB EGFP donor vector (PB-CMV-MCS-EF1ɑ-GFP, #PB511B-1) was purchased from System Biosciences (Palo Alto, CA). The PiggyBac transposase (PBase ...
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bioRxiv - Cell Biology 2023Quote: The CRISPR guide RNAs were cloned into the CMV-hspCas9-H1-gRNA SmartNuclease vector (System Biosciences, Palo Alto, CA, USA). The Cas9-target sequences for PPARγ-binding sites are as follows ...
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bioRxiv - Neuroscience 2021Quote: ... The viral particles were precipitated from the media using AAVanced concentration reagent (System Biosciences #AAV100A-1) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... Media containing virus particles was mixed with PEG-it virus precipitation solution (System Biosciences, #LV810A-1) and incubated at 4°C overnight ...
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bioRxiv - Microbiology 2020Quote: ... two modified PiggyBac (PB) transposon vectors (MIHIPsMie and EIRBsMie) were constructed based on PB-CMV-MCS-EF1α-RedPuro (System Biosciences, PB514B2). The fragment of CMV-MCS-EF1α-RedPuro on this vector was removed by SfiI/ApaI digestion ...
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bioRxiv - Cancer Biology 2020Quote: The piggyBac transposon vector was constructed by inserting a random 10-bp DNA barcode sequence between the CMV promoter and the multiple cloning site of the piggyBac dual promoter vector (PB513B-1; System Biosciences). The GFP sequence in the piggyBac vector was deleted by a site-directed mutagenesis technique for the direct repeat–green fluorescent protein (DR-GFP ...
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bioRxiv - Microbiology 2021Quote: The gene for human HBEGF was synthesized and cloned into the MCS of PiggyBac vector PB-CMV-MCS-EF1α-Puro (System Biosciences) by restriction digest with EcoRI and BamHI (pCMV-hHBEGF) ...
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bioRxiv - Neuroscience 2022Quote: ... dual-reporter lentiviral plasmids pLL410_EF1a-rFLuc-T2A-GFP-mPGK-Puro (LL410PA-1) and pLL-CMV-rFLuc-T2A-GFP-mPGK-Puro (LL310PA-1) were obtained from System Bioscience. Lentiviral generation was carried out as previously described [11] ...