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Citations for Spherotech :
1 - 41 of 41 citations for hsa mir 32 Real Time RT PCR Detection and U6 Calibration Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: ... The Ultra Rainbow Calibration bead kit (Spherotech URCP-38-2k) consists of 3.8 µm polystyrene particles which contain embedded six different fluorochromes that align to the most common flow cytometry excitation and emission filter sets ...
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bioRxiv - Synthetic Biology 2021Quote: ... Calibration beads (Spherotech, RCP30-5A) were measured at the end of each cytometry session ...
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bioRxiv - Cell Biology 2021Quote: ... Calibration of flow cytometer was performed with the three-millimeter rainbow calibration particles (Spherotech, Libertyville, IL) before each run ...
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bioRxiv - Synthetic Biology 2023Quote: ... the fluorescence of calibration beads (Spherotech) was measured to enable convertion of the arbitrary fluorescence units (AFU ...
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bioRxiv - Synthetic Biology 2020Quote: ... Rainbow Calibration Particles (Spherotech #RCP-30-5) or UltraRainbow Calibration Particles (Spherotech #URCP-100-2H ...
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bioRxiv - Bioengineering 2022Quote: ... UltraRainbow calibration beads (Spherotech URCP-38-2K) were used to adjust the photomultiplier tube voltage in each channel to maintain the median fluorescence intensity of the brightest peak within a tolerance of ± 5%.
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bioRxiv - Synthetic Biology 2024Quote: ... UltraRainbow Calibration Particles (Spherotech #URCP-100-2H) were run with each flow cytometry experiment ...
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bioRxiv - Synthetic Biology 2020Quote: ... or UltraRainbow Calibration Particles (Spherotech #URCP-100-2H) were run with each flow cytometry experiment ...
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bioRxiv - Synthetic Biology 2021Quote: ... Fluorescent beads (Rainbow Calibration beads, Spherotech, RCP-30-5A) samples were also acquired during every flow cytometry experiment to enable conversion of data from arbitrary units (a.u. ...
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bioRxiv - Cell Biology 2022Quote: Rainbow Calibration Particles (RCP-05-5, lot AL01, Spherotech) with known Equivalent number of Reference Fluorophores (ERF ...
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bioRxiv - Cell Biology 2019Quote: ... SPHERO Drop Delay Calibration Particles (Spherotech, Lake Forest, IL) were used to confirm the drop targeting ...
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bioRxiv - Synthetic Biology 2022Quote: ... Fluorescence calibration beads (Spherotech, part no. RCP-30-20A) were also measured with each batch of samples to facilitate calibration of flow cytometry data to molecules of equivalent fluorescein (MEF ...
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bioRxiv - Synthetic Biology 2024Quote: ... Fluorescence calibration beads (Spherotech, part no. RCP-30-20A) were also measured with each flow cytometry plate to enable calibration of the flow cytometry data to molecules of equivalent fluorophore5–7.
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bioRxiv - Synthetic Biology 2019Quote: ... Cell autofluorescence was subtracted and MFI was converted to Mean Equivalents of Fluorescein (MEFLs) using the coefficient determined by the calibration curve of UltraRainbow Calibration Particles (Spherotech URCP-100-2H) run in each individual experiment ...
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bioRxiv - Physiology 2022Quote: ... Calibration of the cytometer was assessed using fluorescent particles of standardized size (Nano Fluorescent Particle Size Standard Kit, Spherotech).
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bioRxiv - Developmental Biology 2021Quote: ... Instrument calibration was checked each day with SPHERO Ultra Rainbow (Spherotech) calibration particles ...
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bioRxiv - Immunology 2019Quote: ... the raw data were converted to plate-calibrated ERFs using rainbow calibration particles (Spherotech). For a given cell population and readout ...
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bioRxiv - Bioengineering 2023Quote: ... with size limits determined using fluorescent calibration beads from Spherotech (Lake Forest, IL, USA). For surface protein examination ...
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bioRxiv - Bioengineering 2023Quote: ... size gates were constructed using fluorescent calibration beads from Spherotech (Lake Forest, IL, USA). For experiments using the BD FACSAriaII flow cytometer ...
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bioRxiv - Bioengineering 2023Quote: ... Rainbow Calibration particles (RCP-60-5-2, RCP-60-5-5) were obtained from Spherotech. FITC and PE stained beads were part of the three color BD CaliBRITE kit (BD Biosciences 340486) ...
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bioRxiv - Molecular Biology 2020Quote: ... MFI was then converted to Mean Equivalent of Fluorescien (MEFL) using UltraRainbow Calibration Particles (Spherotech URCP-100-2H), which were incorporated as part of each individual experiment (Supplementary Fig ...
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FACS-Sortable Picoreactors for Ultra High-throughput Screening of Catalysts in Biphasic EnvironmentsbioRxiv - Bioengineering 2024Quote: ... Laser delays were manually calibrated with 32 µm AccuCount Ultra Rainbow beads (Spherotech). Forward and side scattering voltage were manually optimized samples of double emulsions ...
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bioRxiv - Cell Biology 2024Quote: ... Voltages were established using relevant controls and were maintained (+/- 10%) across replicates using calibration particles (Spherotech, URCP-38-2K). Cells were separated from debris by optical gating based off forward and side scatter area ...
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bioRxiv - Synthetic Biology 2020Quote: ... Fluorescence data was calibrated to molecules of equivalent fluorophore (MEF) using fluorescent calibration beads (Spherotech, part no. RCP-30-20A). The cytometer was programmed to measure a 25 μL portion of each cell sample ...
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bioRxiv - Biophysics 2020Quote: ... Fluorescence data was calibrated to molecules of equivalent fluorophore (MEF) using fluorescent calibration beads (Spherotech, part no. RCP-30-20A). The cytometer was programmed to measure a 25 µL portion of each cell sample ...
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bioRxiv - Synthetic Biology 2019Quote: Each team providing flow cytometry data also obtained their own sample of SpheroTech RCP-30-5A Rainbow Calibration Particles (SpheroTech). A sample of this material is a mixture of particles with eight levels of fluorescence ...
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bioRxiv - Molecular Biology 2021Quote: ... Gain of the various channels was optimized by analyzing 8 Peak Rainbow Calibration Beads from Spherotech (Cat. RCP-30-5A). Gain was increased incrementally from 25 to 3000 in each channel ...
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bioRxiv - Synthetic Biology 2020Quote: ... GFPmut3* fluorescence units were converted into standardized units of molecules of equivalent fluorescein (MEFL) using a fluorescent bead standard (Rainbow calibration standard; cat. no. RCP-30-20A, Spherotech, Inc.) as described previously(Castillo-Hair et al. ...
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bioRxiv - Synthetic Biology 2021Quote: ... to enable conversion of GFP fluorescence into calibrated MEFL units 58 a single well per plate contained 15 μL of 8-peak Rainbow Calibration Particles (Spherotech, RCP-30-5A) diluted into 200 μL PBS ...
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bioRxiv - Cell Biology 2020Quote: The molecules of equivalent soluble fluorochrome (MESF) were calculated for PE and CFSE using SPERO TM Rainbow Beads Calibration Particles (Spherotech RCP-05-5, USA) according to instructions provided by the manufacturer ...
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bioRxiv - Cancer Biology 2022Quote: ... and PMT voltages were adjusted to predetermined target channels using the seventh peak of 8-peak Rainbow Calibration Particles (Spherotech, Lake Forest, IL, Cat. No. RCP-30-5A;) as a reference point ...
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bioRxiv - Plant Biology 2019Quote: ... Nanophytoplankton were distinguished from picoplankton based on light scatter (FSC and SSC) and pulse width (transit time) compared to 3 µm UltraRainbow fluorescent particles (Spherotech).
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bioRxiv - Immunology 2021Quote: ... The cells were screened in a 30 min time-course assay for secretion of antibodies that bound to 7 µm streptavidin beads (Spherotech) coated with 10 µg/mL SARS-CoV-2 spike or RBD ...
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bioRxiv - Microbiology 2023Quote: ... Cells were screened in a 30 min time course assay for secretion of antibodies that bound to streptavidin beads (Spherotech) coated with 10µg/mL biotinylated SARS-CoV-2 S-2P ...
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bioRxiv - Molecular Biology 2019Quote: ... We used the SPHERO™ Nano Fluorescent Particle Size Standard Kit, Yellow (diameters 0.22, 0.45, 0.88 and 1.35 μm) from Spherotech Inc ...
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bioRxiv - Immunology 2023Quote: ... recombinant proteins were passively absorbed onto streptavidin functionalized 4-µm fluorescent microparticles (Carboxy Blue Particle Array Kit, Spherotech). 500 µg of biotinylated recombinant protein was incubated with 2 x 107 streptavidin functionalized fluorescent microparticles in 400 µL of 1% BSA in PBS ...
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bioRxiv - Immunology 2022Quote: ... and BA.1 strains of SARS-CoV-2 and biotinylated BSA were incubated for 30 min on ice with different fluorescence intensity peaks of the Streptavidin Coated Fluorescent Yellow Particle Kit (Spherotech) at 9.12 ng per μg beads ...
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bioRxiv - Immunology 2023Quote: ... SA was then conjugated to spherotech 4μm and 5μm Carboxy Blue Particle Array Kits (Spherotech CPAK-4067-8K and CPAK-5067-10K) using 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC ...
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bioRxiv - Immunology 2021Quote: ... and following surface staining cells were fixed and permeabilized according to the manufacturer’s instructions using the BD Cytofix/Cytoperm Plus Kit (BD. For counting the cells AccuCount Fluorecent particles (Spherotech, Lake Forest, IL, USA) were used ...
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bioRxiv - Biochemistry 2023Quote: ... as compared with standard polystyrene beads of 880 nm and 1350 nm size from the SPHEROTM Nano Fluorescent particle standard kit (Spherotech, Lake Forest, IL).37 Marker-positive platelet and microparticle count were expressed as % of all events captured in a joint gate “Platelets + Microparticles” ...
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bioRxiv - Immunology 2023Quote: Custom cytometric bead arrays were generated as previously described87 using 4 µm and 5 µm carboxy functionalized array kits (Spherotech, PAK-4067-8K and PAK-5067-10K). Streptavidin (SA ...