Labshake search
Citations for Spherotech :
1 - 49 of 49 citations for TRNA cytosine 5 Methyltransferase TRDMT1 Antibody Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2023Quote: ... or Biotin-Beads (for lipidomics, Spherotech; TP-30-5; ∼3μm) served as control beads ...
-
bioRxiv - Bioengineering 2022Quote: ... Biotin-coated polystyrene beads (Cat#TPX-150-5) were purchased from Spherotech.
-
bioRxiv - Cell Biology 2020Quote: ... diluted to ~20 nm was mixed with ~1 pm of penta-His-biotin antibody conjugated streptavidin polystyrene beads (0.43 μm or 0.56 μm or 0.89 μm diameter, Spherotech). This ratio produced the bead binding fraction of 10-15% and was maintained to maximize the probability of finding single motor beads in the solution ...
-
bioRxiv - Immunology 2020Quote: ... The antibodies and reagents for FACS analysis are listed below: SPHERO™ AccuCount Fluorescent (Spherotech, Cat.# ACFP-70-5), Anti-Human CD45 APC-Cy7 (HI30 ...
-
bioRxiv - Cell Biology 2023Quote: ... an aliquot of the crosslinked protein-DNA mixture was mixed with 5 μL 2.1 μm diameter anti-digoxigenin antibody coated polystyrene beads (Spherotech) and incubated at room temperature for 15 min ...
-
bioRxiv - Bioengineering 2023Quote: ... Rainbow Calibration particles (RCP-60-5-2, RCP-60-5-5) were obtained from Spherotech. FITC and PE stained beads were part of the three color BD CaliBRITE kit (BD Biosciences 340486) ...
-
bioRxiv - Immunology 2020Quote: ... we used 5 μm streptavidin-coated polystyrene bead (Spherotech #VP-60-5). We used 1,1’-carbonyldiimidazole in DMSO based system as described by Tam et al ...
-
bioRxiv - Molecular Biology 2021Quote: ... Laminar flow separated channels 1-3 were used to form a single-stranded biotin-λ DNA tether as follows: a single 4.38-µm streptavidin-coated polystyrene bead (Spherotech) was caught in each trap in channel 1 (trap stiffness of ∼0.4 pN/nm) ...
-
bioRxiv - Microbiology 2021Quote: ... Nile Red nominally 5 µm (5-7.9 µm) (Spherotech, FP-6056-2, 1.5 × 108/ml) and Nile Red nominally 10 µm (10-14 µm ...
-
bioRxiv - Cell Biology 2021Quote: ... Fluorescent carboxylated 5 µm beads (Spherotech) were added to cells at a ratio of 5:1 and incubated for 6 h at 37°C ...
-
bioRxiv - Immunology 2024Quote: ... Avidin beads (Spherotech, SVP-30-5) were pre-incubated in the same medium with different concentrations of CD4- or CD8-biotin and washed once ...
-
bioRxiv - Synthetic Biology 2020Quote: ... Rainbow Calibration Particles (Spherotech #RCP-30-5) or UltraRainbow Calibration Particles (Spherotech #URCP-100-2H ...
-
bioRxiv - Immunology 2022Quote: ... 7 μm streptavidin beads (Spherotech, SVP-60-5) coated in tandem with 10 μg/mL MERS-CoV spike and 10 μg/mL OC43-CoV spike were re-suspended in a cocktail of 2.5 μg/mL goat anti-human IgG-Alexa Fluor 647 (Jackson ImmunoResearch ...
-
bioRxiv - Biophysics 2023Quote: ... Streptavidin-coated polystyrene beads (Spherotech, SVP-10-5) was used as reference beads for the stage drift correction ...
-
bioRxiv - Biophysics 2021Quote: Protein G-coated polystyrene beads (Spherotech, PGP-60-5) were functionalized with PSGL1-Fc Chimera (R&D systems ...
-
bioRxiv - Cell Biology 2022Quote: Rainbow Calibration Particles (RCP-05-5, lot AL01, Spherotech) with known Equivalent number of Reference Fluorophores (ERF ...
-
bioRxiv - Cell Biology 2023Quote: ... BSA-Beads (for immunofluorescence, Spherotech; BP-30-5; ∼3μm) or Biotin-Beads (for lipidomics ...
-
bioRxiv - Bioengineering 2021Quote: Fluorescent PMMA (PolyAn, 5 μm pink) and PS (Spherotech, 7 μm yellow) microbeads were prepared in standard solutions ...
-
bioRxiv - Biophysics 2022Quote: ... 4.2 µm polystyrene beads coated in streptavidin (Spherotech cat# SVP-40-5) were caught in each trap ...
-
bioRxiv - Bioengineering 2023Quote: ... Pre-rinsed 4.5 µm streptavidin labeled polystyrene particles (Spherotech SVP-40-5) are combined with 1 µM biotinylated oligoes that are a reverse complement to the oligo covalently attached to the slide and incubated for 20 minutes at room temperature ...
-
bioRxiv - Bioengineering 2022Quote: 5 μm in diameter polystyrene beads coated with streptavidin were purchased from Spherotech. The beads were cleaned 3 times in PBS and resuspended in a solution of PBS with 20 μg/ml biotinylated fibronectin (Cytoskeleton ...
-
bioRxiv - Immunology 2023Quote: ... Channels were then flooded with 7 µm streptavidin beads (Spherotech, SVP-60-5) coated with 50 μg/mL of biotinylated RH5 and suspended in a cocktail of 2.5 μg/mL IgG-AF647 ...
-
bioRxiv - Bioengineering 2023Quote: ... a known quantity of ∼5 micron AccuCount Fluorescent Beads (Spherotech, Lake Forest, IL, USA) was used for CHO MP counting.
-
bioRxiv - Cell Biology 2022Quote: Streptavidin-coated polystyrene beads (3 μm in diameter, SVP-30-5, 0.5% w/v, Spherotech) were washed three times in a 10X volume of PBS and recovered by centrifugation (12,000 rpm ...
-
bioRxiv - Biophysics 2022Quote: ... we additionally relied on 3 μm diameter beads (1.0% w/v, Spherotech, cat. SVSIP-30-5).
-
bioRxiv - Biophysics 2024Quote: ... was tethered between 4.38-µm SPHERO Streptavidin Coated polystyrene beads (Spherotech, Cat. No. SVP-40-5) in Trap1 and 2.12-µm SPHERO Anti-Dig polystyrene beads (Spherotech ...
-
bioRxiv - Biophysics 2021Quote: ... we 10-fold diluted a 5% w/v stock of polystyrene beads (PP-50-10, Spherotech, Inc.) and put 5 µm onto an easy cleaned [104] microscope glass slide ...
-
bioRxiv - Immunology 2021Quote: Biotinylated soluble TCR Cl5 (1) or scCl5 were mixed with 5-7μm streptavidin-coated UV-beads (Spherotech) in excess to ensure fully coated beads ...
-
bioRxiv - Biophysics 2020Quote: ... A 10 μl reaction containing 3.5 μL of annealed complex and 1.6 μL of 0.5% streptavidin coated polystyrene beads (2 μm diameter, Spherotech) was incubated on ice for 30 min ...
-
bioRxiv - Biophysics 2022Quote: ... these filaments were mixed with 4 μl of 2.4 μM Neutravidin-coated beads (NVP-20-5, diameter 2.1 μm, Spherotech) and incubated for 15 minutes to couple the filaments to the beads ...
-
bioRxiv - Bioengineering 2023Quote: ... soluble SiRPα-Fc at saturating concentrations were immobilized on 0.4-0.6um Protein G Yellow Fluorescent Particles (PGFP-0552-5, Spherotech) at 4°C for 1h ...
-
bioRxiv - Biophysics 2019Quote: ... 2 or 2.4 µL of 0.05 nM dsDNA were incubated for an hour at room temperature with 5 µL of 0.2% w/v streptavidin-coated beads (Spherotech). Beads were diluted in approximately 300 µL of buffer (100 mM Tris ...
-
bioRxiv - Biophysics 2021Quote: ... and 1 to 1.5 μL of the d-ssDNA solution was incubated for an hour at room temperature with 5 μL of 0.2% w/v streptavidin-coated microspheres (Spherotech). Beads were diluted in approximately 300 μL of buffer (100 mM Tris ...
-
bioRxiv - Biophysics 2023Quote: ... 1% DMSO (Helicon) followed by addition of 10 μl streptavidin-coated microspheres (2.1 μm) (Spherotech, Cat № SVP-20-5). After washing away the unbound microspheres ...
-
bioRxiv - Immunology 2021Quote: ... Cells were counted using Sphero AccuCount 5-5.9 μm beads according to the manufacturers protocol (Spherotech, Cat. #ACBP-50-10).
-
bioRxiv - Biophysics 2021Quote: An optical trap (Tweez305, Aresis, Slovenia) was applied to drag an r = 1.60 μm radius polystyrene bead (HUP-30-5, Spherotech, US) in a large dextran-rich condensate at 13 different velocities (Figure 2e) ...
-
bioRxiv - Biophysics 2022Quote: Streptavidin-coated silica beads of 1.58 μm diameter (1.0% w/v, Spherotech, Lake forest, IL, USA, cat. SVSIP-15-5) were used for the FKBP12-rapamycin-FRB measurement ...
-
bioRxiv - Immunology 2023Quote: ... Cells were counted using Sphero AccuCount 5-5.9 μm beads according to the manufacturer’s protocol (Spherotech, Cat. #ACBP-50-10).
-
bioRxiv - Microbiology 2024Quote: ... No. PP-10-10, 5% w/v, 1.23 µm mean diameter, ∼4.89 × 1010 particles/mL, obtained from Spherotech, Illinois USA), beads were diluted to 108 particles/mL in PBS prior to the addition of virus ...
-
bioRxiv - Biophysics 2021Quote: ... Anti-digoxigenin antibodies were coupled to 1 um polystyrene beads via NHS-EDC coupling: 50 ul of 5% (w/v) 1 um carboxylated polystyrene beads (Spherotech, USA) was buffer exchanged in to reaction buffer (MES-NaOH ...
-
bioRxiv - Microbiology 2023Quote: ... cells were counted by flow cytometry using spike-in of a known amount of AccuCount Blank Particles 5-5.9 µm (Spherotech, ACBP-50-10) as previously described7.
-
bioRxiv - Biophysics 2022Quote: ... and a 2.12 µm polystyrene bead coated in anti-digoxigenin antibody (Spherotech cat# DIGP-20-2) was caught in trap 2 which is upstream in the path of buffer flow to trap 1 ...
-
bioRxiv - Cell Biology 2020Quote: The molecules of equivalent soluble fluorochrome (MESF) were calculated for PE and CFSE using SPERO TM Rainbow Beads Calibration Particles (Spherotech RCP-05-5, USA) according to instructions provided by the manufacturer ...
-
bioRxiv - Microbiology 2024Quote: ... Compensation was calculated by FACS DIVA software 6.1.3 using appropriate single antibody labeled compensation beads from SpheroTech. Data analysis was performed using FlowJo software v9.6 (Treestar).
-
bioRxiv - Immunology 2023Quote: Custom cytometric bead arrays were generated as previously described87 using 4 µm and 5 µm carboxy functionalized array kits (Spherotech, PAK-4067-8K and PAK-5067-10K). Streptavidin (SA ...
-
bioRxiv - Biophysics 2021Quote: ... 1~2 μL dilution of such streptavidin-DNA mixture containing 1~10 ng DNA was mixed with 10 μL anti-digoxigenin antibody coated polystyrene beads 2.1 μm in diameter (Spherotech) and incubated at room temperature for 15 min ...
-
bioRxiv - Molecular Biology 2021Quote: ... constructs were mixed with 4 μl of polystyrene beads coated with antibodies against digoxigenin (AD beads, 0.1% v/v suspension, Ø 1.76 μm, Spherotech), 10 μl of assay buffer (20 mM HEPES ...
-
bioRxiv - Immunology 2021Quote: ... The cells were screened in a 30 min time-course assay for secretion of antibodies that bound to 7 µm streptavidin beads (Spherotech) coated with 10 µg/mL SARS-CoV-2 spike or RBD ...
-
bioRxiv - Microbiology 2023Quote: ... Cells were screened in a 30 min time course assay for secretion of antibodies that bound to streptavidin beads (Spherotech) coated with 10µg/mL biotinylated SARS-CoV-2 S-2P ...