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Citations for Spherotech :
1 - 50 of 75 citations for Recombinant Yellow Fluorescent Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: Recombinant biotinylated XBB.1.5 spike protein was incubated with SPHERO Streptavidin fluorescent Yellow Particles (Spherotech, Cat. #SVF3- 2552) at 20 ng per μg beads for 30 min at room temperature on an end-over-end mixer ...
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bioRxiv - Synthetic Biology 2021Quote: ... or carboxyl polystyrene fluorescent yellow particles (Spherotech, Inc.) in 100 µl 1x PBS were run as a negative control.
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bioRxiv - Bioengineering 2023Quote: ... soluble SiRPα-Fc at saturating concentrations were immobilized on 0.4-0.6um Protein G Yellow Fluorescent Particles (PGFP-0552-5, Spherotech) at 4°C for 1h ...
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bioRxiv - Bioengineering 2021Quote: The fluorescent yellow microbeads (10.2 μm; Spherotech, Lake Forest, IL, USA) were incubated with Pierce protein-free blocking buffer (Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2021Quote: Fluorescent PMMA (PolyAn, 5 μm pink) and PS (Spherotech, 7 μm yellow) microbeads were prepared in standard solutions ...
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bioRxiv - Developmental Biology 2021Quote: ... Yellow fluorescent carboxyl magnetic particles that are 0.5 μm in diameter (Spherotech) were diluted in water (1:10 ...
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bioRxiv - Biophysics 2021Quote: 0.5 μm polystyrene fluorescent beads (FP-0545-2; SPHERO Fluorescent Particles, Light Yellow, 0.4-0.6 μm; Spherotech, USA) were injected at the 1-cell stage into the cytoplasm-yolk interface ...
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bioRxiv - Immunology 2023Quote: ... recombinant proteins were passively absorbed onto streptavidin functionalized 4-µm fluorescent microparticles (Carboxy Blue Particle Array Kit, Spherotech). 500 µg of biotinylated recombinant protein was incubated with 2 x 107 streptavidin functionalized fluorescent microparticles in 400 µL of 1% BSA in PBS ...
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bioRxiv - Synthetic Biology 2021Quote: ... and 2 µl of 1% w/v amine polystyrene fluorescent yellow particles (NH2-beads, Spherotech, Inc) or carboxyl polystyrene fluorescent yellow particles (Spherotech ...
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bioRxiv - Molecular Biology 2019Quote: ... We used the SPHERO™ Nano Fluorescent Particle Size Standard Kit, Yellow (diameters 0.22, 0.45, 0.88 and 1.35 μm) from Spherotech Inc ...
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bioRxiv - Immunology 2022Quote: ... and BA.1 strains of SARS-CoV-2 and biotinylated BSA were incubated for 30 min on ice with different fluorescence intensity peaks of the Streptavidin Coated Fluorescent Yellow Particle Kit (Spherotech) at 9.12 ng per μg beads ...
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bioRxiv - Immunology 2024Quote: ... and XBB.1.5 strains of SARS-CoV-2 were incubated with different fluorescence intensity peaks of the SPHERO Streptavidin fluorescent Yellow Particles (Spherotech, Cat. #SVF3-2552) at 20 ng per μg beads for 30 min at room temperature on an end-over-end mixer ...
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bioRxiv - Biophysics 2021Quote: ... 0.06 μm Carboxyl FPS yellow particles (Spherotech) were briefly sonicated and diluted right before use in Dulbecco’s Phosphate Buffered Saline (DPBS ...
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bioRxiv - Microbiology 2022Quote: Purified biotinylated proteins were multimerised on streptavidin coated Nile Red fluorescent beads (Spherotech Inc ...
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bioRxiv - Immunology 2020Quote: ... recombinant SARS-CoV-2 ectodomain trimers were passively absorbed onto streptavidin functionalized fluorescent microparticles (Spherotech 3.6um cat# CPAK-3567-4K, peak 4). 500 µg of biotinylated SARS2-CoV-2 was incubated with 2E+07 Streptavidin functionalized fluorescent microparticles in 400μl of 1% BSA in PBS ...
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bioRxiv - Immunology 2021Quote: ... fluorescent counting beads (AccuCount Fluorescent Particles from Spherotech) were added before analysis to calculate the number of donor Pmel-1 CD8+ T cells ...
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bioRxiv - Cancer Biology 2021Quote: ... Fluorescent beads (Spherotech) were then added at a volume ratio of 1:10,000 to label the Matrigel for imaging ...
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bioRxiv - Cancer Biology 2021Quote: ... Fluorescent beads (Spherotech) were then added at a volume ratio of 1:10,000 to label the Matrigel for imaging ...
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bioRxiv - Cancer Biology 2019Quote: ... AccuCount fluorescent particles (Spherotech) were added to the tumor digests.
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bioRxiv - Microbiology 2022Quote: ... AccCount Fluorescent Particles (Spherotech) were added to each sample to evaluate the total number of cells in the sample during flow cytometry analysis ...
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bioRxiv - Cancer Biology 2023Quote: ... AccuCount Fluorescent particles (Spherotech) were used for counting the cells.
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bioRxiv - Cell Biology 2023Quote: ... We then incubated each sample with 0.22-μm light yellow beads (Spherotech, FP-0245-2) resuspended in 2× SSC as fiducial markers for drift correction and washed the sample with 2× SSC briefly.
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bioRxiv - Neuroscience 2019Quote: ... fluorescent microspheres (Spherotech, FP4060-2) were imaged following the same procedures used for biocytin filled cells (Figure S5).
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bioRxiv - Cancer Biology 2022Quote: ... 10,000 AccuCount fluorescent particles (Spherotech) were added to each sample ...
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bioRxiv - Immunology 2023Quote: ... SPHERO ACCUCOUNT fluorescent beads (Spherotech) were added for quantification purposes ...
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bioRxiv - Microbiology 2022Quote: ... green fluorescent aminated (radius = 50 nm) or green fluorescent carboxylated (radius = 60 nm) polystyrene nanoparticles (Spherotech) were gently pipetted directly below the liquid-air interface to avoid structural disturbance of the biofilms ...
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bioRxiv - Cell Biology 2021Quote: ... Fluorescent carboxylated 5 µm beads (Spherotech) were added to cells at a ratio of 5:1 and incubated for 6 h at 37°C ...
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bioRxiv - Immunology 2022Quote: ... 0.2 μm Sky Blue fluorescent beads (Spherotech) were administered i.n ...
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bioRxiv - Bioengineering 2023Quote: ... 10 µL of AccuCount fluorescent beads (Spherotech) was added to each depleted blood sample following CD41a antibody incubation ...
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bioRxiv - Physiology 2022Quote: ... Calibration of the cytometer was assessed using fluorescent particles of standardized size (Nano Fluorescent Particle Size Standard Kit, Spherotech).
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bioRxiv - Bioengineering 2019Quote: ... Rainbow fluorescent beads (Spherotech, Cat. # RFP-30-5A) were used as fluorescence intensity standards ...
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bioRxiv - Microbiology 2021Quote: SPHERO carboxyl magnetic blue fluorescent beads (Spherotech, USA) were coupled with SARS-CoV-2 whole spike protein (Lake Pharma ...
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bioRxiv - Cell Biology 2023Quote: ... All fluorescent polystyrene microbeads were purchased from Spherotech Inc ...
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Sex and species associated differences in Complement-mediated immunity in Humans and Rhesus macaquesbioRxiv - Immunology 2023Quote: ... ARP-12026) was to a 400 µL volume of fluorescent beads (1.7-2.2-micron SPHERO Carboxyl Fluorescent Particles, Spherotech Inc, CFP-2052-2) using carbodiimide chemistry as described previously (Chu ...
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bioRxiv - Synthetic Biology 2021Quote: ... Fluorescent beads (Rainbow Calibration beads, Spherotech, RCP-30-5A) samples were also acquired during every flow cytometry experiment to enable conversion of data from arbitrary units (a.u. ...
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bioRxiv - Cell Biology 2020Quote: ... diameter of 3.0 mm (Rainbow Fluorescent Particle Slide from Spherotech). A second fine adjustment of the alignment was done at the beginning of the image processing.
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bioRxiv - Bioengineering 2023Quote: ... 106/mL AccuCount Fluorescent Beads (Spherotech, Lake Forest, IL, USA) was added to each sample for MkEV quantification ...
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bioRxiv - Immunology 2020Quote: ... antibodies in PBS were mixed with 5E+07 fluorescent microparticles each with a unique fluorescent intensity in the far red channels (Spherotech 3.6um cat# CPAK-3567-4K, peaks 1-3) resuspended in 0.1 M MES buffer pH 5.0 ...
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bioRxiv - Immunology 2021Quote: ... and cell numbers were quantified using PE-conjugated fluorescent counting beads (Spherotech).
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bioRxiv - Microbiology 2020Quote: ... and cell numbers were quantified using PE-conjugated fluorescent counting beads (Spherotech).
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bioRxiv - Neuroscience 2022Quote: ... of 500 nm diameter fluorescent polystyrene beads (Spherotech, Inc., FICP-08-2) embedded in agarose placed inside a quartz cuvette (Thorlabs ...
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bioRxiv - Immunology 2023Quote: ... Voltages on the machine were standardized using fluorescent targets and rainbow beads (Spherotech). Data were analyzed with FlowJo software (v10.7.1 ...
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bioRxiv - Neuroscience 2020Quote: ... microglia was incubated with ANXV (1:20) for 15’ and fluorescent particles (3um) (Spherotech) were added to microglial cells at a 1:1 microglia/beads ratio for 2hr ...
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bioRxiv - Bioengineering 2023Quote: ... a known quantity of ∼5 micron AccuCount Fluorescent Beads (Spherotech, Lake Forest, IL, USA) was used for CHO MP counting.
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bioRxiv - Bioengineering 2023Quote: ... size gates were constructed using fluorescent calibration beads from Spherotech (Lake Forest, IL, USA). For experiments using the BD FACSAriaII flow cytometer ...
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bioRxiv - Bioengineering 2023Quote: ... with size limits determined using fluorescent calibration beads from Spherotech (Lake Forest, IL, USA). For surface protein examination ...
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bioRxiv - Immunology 2021Quote: ... Frequency calculation was obtained by mixing 1/10th of sample with 200,000 fluorescent beads (Spherotech) for normalization (16) ...
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bioRxiv - Microbiology 2020Quote: ... Each cytometer run was calibrated with 1 mm diameter fluorescent Ultra Rainbow beads (Spherotech Inc.). 100 μL for autofluorescent cells and 75 μL for heterotrophic cells were run at an average flow rate of 20 μL min−1 and monitored with a liquid flowmeter (Sensirion US) ...
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bioRxiv - Immunology 2020Quote: ... laser voltages were standardized using SPHERO™ Ultra Rainbow Fluorescent Particles (Spherotech #URFP01-30-2K). Data from at least 10,000 single bacterial events were measured and analyzed by FlowJo software (v10.0.7).
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bioRxiv - Immunology 2020Quote: ... Routinely alignments were performed with Ultra Rainbow Fluorescent Particles 3.0 µm (Spherotech, URFP-30-2). The triggering threshold was applied to the side scatter on the 488-SSC channel and the relative applied voltage was determined by using size reference beads (Apogee flow systems ...