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Citations for National Diagnostics :
1 - 50 of 63 citations for Anti Flag Affinity Gel since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2020Quote: Polyacrylamide gels were prepared with the SequaGel - Urea Gel system (National Diagnostics). A 50 ml mix to yield a 20% gel was degassed under house vacuum with stirring for ∼10 minutes before the addition of 10 μl tetramethylethylenediamine (TEMED ...
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bioRxiv - Molecular Biology 2021Quote: Polyacrylamide gels (20%) were prepared with the SequaGel − Urea Gel system (National Diagnostics) and cast at 0.75 mm thick (1.5 mm for preparative ...
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bioRxiv - Microbiology 2023Quote: TBE-Urea gels were made using SequaGel UreaGel 29:1 Denaturing Gel System (National Diagnostics). 15% or 22.5% gels were poured as indicated in each experiment ...
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bioRxiv - Developmental Biology 2023Quote: Samples were subjected to 15% denaturing polyacrylamide-mediated gel (UreaGel 29:1 gel system, National Diagnostics) and visualized by SYBR gold (ThermoFisher ...
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bioRxiv - Microbiology 2023Quote: ... 15% TBE-Urea gels were made using SequaGel UreaGel 29:1 Denaturing Gel System (National Diagnostics) Urea was added to the samples to a final concentration of 4M before running on the gels at 200V for 90 minutes ...
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bioRxiv - Microbiology 2024Quote: ... All samples were then gel purified on a denaturing 10% polyacrylamide gel (National Diagnostics, EC-829). Gels were cut between 60 to 200 nt and purified via crush and soak overnight tumble at 4° C [crush gel through 18 gauge needle punched bottom of 500 μl tubes into 1.7 mL Eppendorf tubes ...
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bioRxiv - Microbiology 2023Quote: ... PCR products were purified using gel extraction from a 1.0% low-melt agarose gel (National Diagnostics, Atlanta, GA, USA) with a ZR-96 Zymoclean DNA Recovery Kit (Zymo Research ...
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bioRxiv - Biochemistry 2021Quote: ... Labeling reactions were resolved by denaturing gel electrophoresis in a 15% polyacrylamide gel containing 8 M urea (SequaGel, National Diagnostics). 3′ end-labeled RNA was visualized by autoradiography and passively eluted from gel slices overnight at 4°C in 0.3 M NaCl ...
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bioRxiv - Developmental Biology 2022Quote: ... Resolving gels: 6-12% ProtoGel (National Diagnostics EC8901LTR), 0.375 M Tris-HCl (pH 8.8) ...
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bioRxiv - Plant Biology 2022Quote: ... urea diluent and urea gel buffer (National Diagnostics). Ten percent ammonium persulfate (APS ...
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bioRxiv - Biochemistry 2022Quote: ... The samples were then assessed by Urea-Page using an 8% gel prepared with the SequaGel UreaGel 19:1 Denaturing Gel System (National Diagnostics) for a Mini-PROTEAN Tetra Vertical Electrophoresis Cell exactly as described previously (34).
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bioRxiv - Molecular Biology 2023Quote: ... Denaturing Urea-PAGE was performed using 8 or 10% gels prepared using the SequaGel UreaGel 19:1 Denaturing Gel System (National Diagnostics) for a Mini-PROTEAN Tetra Vertical Electrophoresis Cell as described previously52 ...
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bioRxiv - Molecular Biology 2020Quote: ... The samples were run on 8% sequencing gel (National Diagnostics) and visualized by phosphorimager analysis.
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bioRxiv - Genomics 2020Quote: ... on a 20% TBE-Urea PAGE gel (SequaGel, National Diagnostics) and staining with SYBR Gold (ThermoFisher) ...
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bioRxiv - Cell Biology 2021Quote: ... Gels were run in Running buffer (National Diagnostics, Cat # EC870). Protein transfer was done using the GENIE Electrophoretic Transfer (IdeaScientific ...
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bioRxiv - Microbiology 2022Quote: ... and loaded on a urea-PAGE gel (Sequagel, National Diagnostics). Samples were run for 1-2 hours at 100V in 1X TBE buffer ...
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bioRxiv - Molecular Biology 2019Quote: ... and the gels stained with coomassie blue (ProtoBlue, National Diagnostics). The remaining eluate from two independent replicates was electrophoresed approximately 1 cm into a 4-12% NuPage and the top piece of the gel excised and prepared for mass spectrometry analysis as described below ...
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Vps18 contributes to phagosome membrane integrity in Mycobacterium tuberculosis-infected macrophagesbioRxiv - Microbiology 2023Quote: ... Lysates were run on a 10% acrylamide gel (National Diagnostics) in 1X Tris-Glycine SDS Running Buffer (Boston BioProduct ...
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bioRxiv - Molecular Biology 2021Quote: ... RNA was visualised on polyacrylamide 7.5 M urea gel (National Diagnostics) in TBE using SybrGold (Invitrogen).
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bioRxiv - Microbiology 2022Quote: ... then run on an 8% Urea-polyacrylamide gel (SequaGel, National Diagnostics) at 40W for 2.5 hours ...
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bioRxiv - Cell Biology 2020Quote: ... The gels were stained with Coomassie Brilliant Blue R-250 (National Diagnostics) and scanned by an Epson Perfection V700 scanner at 300 dpi ...
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bioRxiv - Molecular Biology 2021Quote: ... and resolved on an 8% acrylamide denaturing gel (National Diagnostics, EC-829). RNA was transferred onto Hybond-NX membranes (GE Healthcare ...
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bioRxiv - Molecular Biology 2021Quote: ... RNAs were separated on 5% acrylamide denaturing gels (National Diagnostics, EC-829). Desired RNA bands were identified by UV-shadowing ...
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bioRxiv - Biochemistry 2023Quote: ... Gels were run at 150 volts in1x Tris/Glycine/SDS (National diagnostics) running buffer ...
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bioRxiv - Developmental Biology 2022Quote: ... Total RNA was run through a 15% denaturing urea-polyacrylamide gel (National Diagnostics) to isolate 15-29 nt small RNAs and remove the 30-nt 2S rRNA ...
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bioRxiv - Biochemistry 2023Quote: SDS-PAGE gels were prepared using Protogel 30% (National Diagnostics, Cat. # EC-890) at varying percentages of acrylamide ranging from 10-12% ...
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bioRxiv - Molecular Biology 2023Quote: ... and resolved on an 8% urea-acrylamide denaturing gel (National Diagnostics, EC-828). The band migrating at the size of 138 nt was identified by UV-shadowing ...
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bioRxiv - Genomics 2019Quote: ... Small RNAs were separated by 8% denaturing polyacrylamide gel electrophoresis (National Diagnostics EC-833) and electroblotted/UV crosslinked to Hybond N+ membrane (GE Healthcare RPN303B) ...
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bioRxiv - Molecular Biology 2022Quote: ... Synthesized GlmZ RNAs were purified on 6% 7.5 M urea polyacrylamide gel (National Diagnostics). The bands were visualized under a portable UV lamp at 254 nm wavelength and excised ...
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bioRxiv - Biochemistry 2021Quote: 20% polyacrylamide gels were prepared using the SequaGel–UreaGel system (National Diagnostics, Atlanta, GA). The gels were allowed to pre-run at a constant power of 20 W for 40−45 mins ...
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bioRxiv - Immunology 2023Quote: ... 20 μg of protein was separated on 12% ProtoGel Quick-Cast Gels (National Diagnostics) before transfer to nitrocellulose membranes (BioRad) ...
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bioRxiv - Biochemistry 2022Quote: ... The gel was stained using ProtoStain™ Blue Colloidal Coomasie G-250 stain (National Diagnostics). For the Western Blot analysis ...
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bioRxiv - Molecular Biology 2021Quote: ... RNA generated by IVT was purified: IVT products were separated on polyacrylamide denaturing gel (National Diagnostics), relevant bands were excised using UV shadowing and electroeluted in TBE (Whatman Elutrap) ...
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bioRxiv - Biochemistry 2019Quote: ... 15% SDS gels were prepared (Separation buffer: 0.38 M Tris pH 8.8, 15% acrylamide(National Diagnostics), 0.1% SDS ...
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bioRxiv - Cancer Biology 2019Quote: ... Ribosome-protected RNA fragments were loaded onto denaturing 17% urea-PAGE gel (EC-829, National Diagnostics) and gel area ranging from 27 nt to 30 nt ...
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bioRxiv - Cell Biology 2022Quote: ... The size markers were resolved on a 15% urea-PAGE gel (National Diagnostics, EC830, EC835, EC840), extracted ...
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bioRxiv - Microbiology 2023Quote: ... The 10 uL reaction mixture was loaded onto an 8% polyacrylamide/8M urea gel (National Diagnostics) alongside a Ambion Decades Markers System ladder that was radiolabeled per the manufacturer’s instructions (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2023Quote: ... Samples were resolved using a 10% denaturing urea polyacrylamide gel (SequaGel®, National Diagnostics, #EC-833). After the gel run ...
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bioRxiv - Molecular Biology 2020Quote: All RNA degradation experiments were quantified via urea polyacrylamide gel electrophoresis (National Diagnostics, EC-830 & EC-840) followed by imaging on a Typhoon 9410 (GE Healthcare) ...
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bioRxiv - Microbiology 2022Quote: ... 5 μg of RNA was ran on a 8% polyacrylamide urea gel (National Diagnostics SequaGel 1:4) in 1x TBE buffer (100mM Tris base ...
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bioRxiv - Microbiology 2023Quote: ... The PCR products were purified by running on a 1% low-melt agarose gel (National Diagnostics, Atlanta, GA) stained with SYBR Safe DNA Gel Stain (Invitrogen ...
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bioRxiv - Molecular Biology 2023Quote: ... 16% 1-mm thick denatured second-dimension gel was prepared using the UreaGel System (National Diagnostics, EC-833). The second-dimension gel was poured to encapsulate the gel slices from the first-dimension gel ...
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bioRxiv - Molecular Biology 2023Quote: ... 8% 1-mm thick denatured first-dimension gel was prepared using the UreaGel System (National Diagnostics, EC-833). A 10-µL sample of fragmented RNA was run on the first-dimension gel in 0.5x Novex TBE running buffer (Invitrogen ...
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bioRxiv - Molecular Biology 2022Quote: ... and run on an 8% denaturing gel made using reagents from the SequaGel® UreaGel™ System (National Diagnostics). Gels were stained with SYBR™ Gold Nucleic Acid Gel Stain (Invitrogen ...
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bioRxiv - Genetics 2023Quote: ... 8µl of each reaction was loaded onto a 6% polyacrylamide gel with 7.5M urea prepared according to manufacturer’s instructions (National Diagnostics SEQUAGEL SEQUENCING SYSTEM 2.2 (Cat# EC-833)) ...
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bioRxiv - Biochemistry 2020Quote: ... 4-5 μg of RNA was separated in a 10% denaturing Urea-polyacrylamide gel (20 × 25 cm; Sequagel, National Diagnostics). The RNA was blotted on Hybond-N+ membranes (GE Healthcare ...
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bioRxiv - Microbiology 2021Quote: ... was separated on denaturing 8% polyacrylamide gels containing 6 M urea (1:4 mix of Ureagel Complete to Ureagel-8 (National Diagnostics) with 0.08% ammonium persulfate ...
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bioRxiv - Microbiology 2020Quote: ... 5 μg of RNA were fractionated on 8% polyacrylamide urea gels containing 6 M urea (1:4 mix of Ureagel Complete to Ureagel-8 (National Diagnostics) with 0.08% ammonium persulfate ...
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bioRxiv - Microbiology 2021Quote: ... of total RNA were fractionated on 8% polyacrylamide urea gels containing 6 M urea (1:4 mix of Ureagel Complete to Ureagel-8 (National Diagnostics) with 0.08% ammonium persulfate ...
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bioRxiv - Microbiology 2020Quote: 20 μg of total RNA and P32 ATP end-labeled Century+ RNA markers were loaded onto 5% denaturing urea polyacrylamide gels (SequaGel, National Diagnostics) and run at 30 watts for 1.5 hours to ensure well-spaced gel migration from 50 to 1,000 nucleotides (nt) ...