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1 - 35 of 35 citations for IL 21 Mouse 129a.a since 2019

• Cholesterol promotes both head group visibility and clustering of PI(4,5)P₂ driving unconventional secretion of Fibroblast Growth Factor 2

  Fabio Lolicato, et al., bioRxiv - Biophysics 2021

  Quote: ... using a master mix containing 50 pmol phosphatidylcholine (PC)-standard mix (PC 13:0/13:0, 14:0/14:0, 2.0:0/20:0; 21:0/21:0; Avanti Polar Lipids) and 150 pmol D7-cholesterol (Avanti Polar Lipids) ...
• A short perinuclear amphipathic α-helix in Apq12 promotes nuclear pore complex biogenesis

  Wanlu Zhang, et al., bioRxiv - Cell Biology 2021

  Quote: ... Homogenized cells were subjected to acidic Bligh&Dyer lipid extractions in the presence of internal lipid standards added from a master mix containing PC (phosphatidylcholine, 13:0/13:0, 14:0/14:0, 20:0/20:0; 21:0/21:0, Avanti Polar Lipids), PI (phosphatidylinositol ...
• Oncogenic memory underlying minimal residual disease in breast cancer

  Ksenija Radic Shechter, et al., bioRxiv - Cancer Biology 2020

  Quote: Acidic extractions were performed as described31 in the presence of an internal lipid standard mix containing 50 pmol phosphatidylcholine (13:0/13:0, 14:0/14:0, 20:0/20:0; 21:0/21:0, Avanti Polar Lipids), 50 pmol sphingomyelin (d18:1 with N-acylated 13:0 ...
• The glycolipid GlcCer is recruited into the viral envelope to promote phenuivirus binding to host cells

  Zina M. Uckeley, et al., bioRxiv - Microbiology 2022

  Quote: ... the master mix consisted of 50 pmol PC (13:0/13:0, 14:0/14:0, 20:0/20:0, and 21:0/21:0, Avanti Polar Lipids), 50 pmol SM (d18:1 with semisynthesized N-acylated 13:0 ...
• The Ebola virus VP40 matrix undergoes endosomal disassembly essential for membrane fusion

  Sophie L. Winter, et al., bioRxiv - Microbiology 2022

  Quote: ... using a master mix consisting of 50 pmol phosphatidylcholine (PC, 13:0/13:0, 14:0/14:0, 20:0/20:0; 21:0/21:0, Avanti Polar Lipids), 50 pmol sphingomyelin (SM ...
• Intramembrane protease SPP defines a cholesterol-regulated abundance control of the mevalonate pathway enzyme SQS

  Dönem Avci, et al., bioRxiv - Cell Biology 2023

  Quote: ... using a master mix containing phosphatidylcholine (13:0/13:0, 14:0/14:0, 20:0/20:0; 21:0/21:0; Avanti Polar Lipids, Alabaster, AL, USA), sphingomyelin (SM ...
• Oncogenic memory underlying minimal residual disease in breast cancer

  Ksenija Radic Shechter, et al., bioRxiv - Cancer Biology 2020

  Quote: ... 10 pmol phosphatidic acid (21:0/22:6, Avanti Polar Lipids), 10 pmol phosphatidylglycerol (14:1/14:1 ...
• Lateral membrane organization as target of an antimicrobial peptidomimetic compound

  Adéla Melcrová, et al., bioRxiv - Biophysics 2023

  Quote: ... Formed liposomes were extruded 21 times through 0.4 μm pores polycarbonate membranes (Avanti Polar Lipids). The extruded liposomes were stored in 4°C overnight.
• Next-generation intranasal Covid-19 vaccine: a polymersome-based protein subunit formulation that provides robust protection against multiple variants of concern and early reduction in viral load of the upper airway in the golden Syrian hamster model

  Jian Hang Lam, et al., bioRxiv - Immunology 2022

  Quote: ... The resulting solution was extruded 21 times through a 200 nm membrane filter (Avanti Polar Lipids) using a 1 ml mini-extruder (Avanti Polar Lipids ...
• Cholesterol in the cargo membrane reduces kinesin-1 binding to microtubules in the presence of tau

  Qiaochu Li, et al., bioRxiv - Biophysics 2022

  Quote: ... and passed through a 30-nm polycarbonate membrane 21 times using a mini-extruder (Avanti Polar Lipids) to generate small unilamellar vesicles ...
• Lateral membrane organization as target of an antimicrobial peptidomimetic compound

  Adéla Melcrová, et al., bioRxiv - Biophysics 2023

  Quote: ... The resuspended liposomes were then extruded 21 times through 0.1 μm pores polycarbonate membranes (Avanti Polar Lipids). The liposomes were stored in 4°C and used for the maximum of one week.
• Metabolomics profiling reveals new aspects of dolichol biosynthesis in Plasmodium falciparum

  Flavia M. Zimbres, et al., bioRxiv - Biochemistry 2020

  Quote: ... Polyprenols and dolichols mixtures of 13 to 21 isoprene units were obtained from Avanti Polar Lipids (Alabaster, AL, USA). Polyprenols of 11 and 12 isoprene units were obtained from Isoprenoids LC (Tampa ...
• The ESCRT-III machinery participates in the production of extracellular vesicles and protein export during Plasmodium falciparum infection

  Yunuen Avalos-Padilla, et al., bioRxiv - Microbiology 2020

  Quote: ... All lipids were obtained from Avanti Polar Lipids (Alabaster, IL, USA).
• A PI3K-WIPI2 positive feedback loop allosterically activates LC3 lipidation in autophagy

  Dorotea Fracchiolla, et al., bioRxiv - Biochemistry 2020

  Quote: ... The resuspended SUVs were then extruded 21 times through a 0.4 μm membrane followed by 21 times through a 0.1 μm membrane (Whatman, Nucleopore, UK) using the Mini Extruder from Avanti Polar Lipids Inc. ...
• Cholesterol in the cargo membrane reduces kinesin-1 binding to microtubules in the presence of tau

  Qiaochu Li, et al., bioRxiv - Biophysics 2022

  Quote: ... The lipid mixture was then extruded through a 100-nm polycarbonate membrane 21 times using a mini-extruder (Avanti Polar Lipids). Lipid vesicles were used immediately following extrusion.
• The ion permeability of DNA nanotube channels

  Naresh Niranjan Dhanasekar, et al., bioRxiv - Biophysics 2022

  Quote: ... The solution was freeze-thawed at least 6 times under liquid nitrogen and then extruded 21 times using an Avanti pressure extruder with Whatmanfilter paper of size 0.1 μm (Avanti Polar Lipids Alabama ...
• Molecular mechanism of mitochondrial phosphatidate transfer by Ups1/Mdm35

  Jiuwei Lu, et al., bioRxiv - Biochemistry 2019

  Quote: ... This freeze-thaw cycle was repeated 5 times and then the thawed solution was extruded 21 times through a 0.1 μM filter using a mini-extruder (Avanti Polar Lipids).
• Polymerization cycle of actin homolog MreB from a Gram-positive bacterium

  Wei Mao, et al., bioRxiv - Biochemistry 2022

  Quote: ... The rehydrated lipid solutions were extruded 21 times through a 100 nm diameter pore size polycarbonate membrane (Avanti Polar Lipids Inc.). The extruded solutions were stored at 4°C and consumed within a week after preparation.
• Multiple Intermediates in the Detergent-Induced Fusion of Lipid Vesicles

  L. G. Dresser, et al., bioRxiv - Biophysics 2023

  Quote: ... The solutions were then extruded at least 21 times through a 200 nm cut off polycarbonate membrane filter (Avanti Polar Lipids). Vesicles were then filtered using PD-10 desalting columns (Sigma Aldrich ...
• ORP5/8 AND MIB/MICOS LINK ER-MITOCHONDRIA AND INTRAMITOCHONDRIAL CONTACTS FOR NON-VESICULAR TRANSPORT OF PHOSPHATIDYLSERINE

  Vera F. Monteiro-Cardoso, et al., bioRxiv - Cell Biology 2022

  Quote: ... Unilamellar liposomes were produced by seven freeze-thaw cycles (30 sec in liquid nitrogen followed by 5 min in a 37°C water bath) and extrusion (at least 21 times) through a polycarbonate filter with 100 nm pore size (polycarbonate membranes from Avanti Polar Lipids). The liposomes were then stored on ice.
• Extracellular vesicle fusion visualized by cryo-EM

  Mattia I. Morandi, et al., bioRxiv - Biophysics 2022

  Quote: ... The resulting MLV suspension was then sonicated for 10 min to disperse larger aggregates and the liposomal suspension was extruded 21 times through polycarbonate filters (100 nm pore size, Avanti Polar Lipids) using a mini-extruder (Avanti Polar Lipids) ...
• Endophilin A1 promotes Actin Polymerization in response to Ca²⁺/calmodulin to Initiate Structural Plasticity of Dendritic Spines

  Yanrui Yang, et al., bioRxiv - Cell Biology 2020

  Quote: ... Liposomes were then extruded 21 times through a polycarbonate membrane with a 50◻nm pore size (Mini-Extruder, Avanti Polar Lipids). Extruded liposomes were centrifuged at 18,000 g for 5 min to remove insoluble material and stored at 4°C ...
• Tunable biomimetic bacterial membranes from binary and ternary lipid mixtures and their application in antimicrobial testing

  Emilia Krok, et al., bioRxiv - Biophysics 2023

  Quote: ... The lipid suspension containing multilamellar vesicles was extruded 21 times through a polycarbonate membrane with a 100 nm pore diameter using a mini-extruder from Avanti Polar Lipids.
• Anionic phospholipids stimulate the proton pumping activity of the plant plasma membrane P-type H⁺-ATPase

  Laura C. Paweletz, et al., bioRxiv - Biochemistry 2023

  Quote: ... The vesicle suspension was further processed by five freeze-thawing cycles (90 s in liquid nitrogen followed by 90 s in a 60°C water bath) and extrusion (21 times) through a stack of polycarbonate membranes (pore size 200 nm) using a mini-extruder (Avanti Polar Lipids). The vesicles made from PC:PE were unstable (lipids felt out of solution after short term storage) ...
• In vitro reconstitution reveals membrane clustering and double-stranded RNA recruitment by the enteroviral AAA+ ATPase 2C

  Kasturika Shankar, et al., bioRxiv - Microbiology 2023

  Quote: ... this solution was passed 21 times through an extrusion membrane (Whatman Nuclepore) with pore size of 2 μm on a mini-extruder (Avanti Polar Lipids) to get a clear solution comprising of LUVs ...
• Probing neuropeptide volume transmission in vivo by a novel all-optical approach

  Hejian Xiong, et al., bioRxiv - Neuroscience 2021

  Quote: ... Afterwards the suspension was extruded through 200 and 100 nm polycarbonate membranes (Whatman, USA) for 21 passages each using a Mini Extruder (Avanti Polar Lipids, USA). Free calcein or SST was removed by size-exclusion chromatography with Sephacryl® S-1000 column (Cat ...
• Structural flexibility of apolipoprotein E-derived arginine-rich peptides improves their cell penetration capability

  Yuki Takechi-Haraya, et al., bioRxiv - Biophysics 2023

  Quote: ... the suspension was extruded 21 times through a mini-extruder equipped with a 0.1-μm polycarbonate filter (Avanti Polar Lipids, Alabaster, AL, USA) at 70 °C.
• Quantitative parameters of bacterial RNA polymerase open-complex formation, stabilization and disruption on a consensus promoter

  Subhas C. Bera, et al., bioRxiv - Biophysics 2021

  Quote: ... with occasional vortexing and subsequently extruded 21 times through 0.1 µm polycarbonate membrane (Avanti, Cat # 610005) using a mini-extruder (Avanti Polar Lipids, USA, Cat # 610023). SUVs stock solution was stored at + 4 ºC for several weeks.
• Drug repurposing screens identifies compounds that inhibit α-synuclein oligomers’ membrane disruption and block antibody interactions

  Arun Kumar Somavarapu, et al., bioRxiv - Biophysics 2022

  Quote: ... subjected to 10 freeze-thaw cycles using liquid nitrogen and a 50 °C water bath and then passed through a mini-extruder 21 times using a 100 nm cut-off filter (Avanti Polar Lipids, Alabaster, AL). DOPG liposomes for calcein release experiments were prepared in the presence of 70 mM calcein (self-quenching concentrations) ...
• Disulfide bridge-dependent dimerization triggers FGF2 membrane translocation into the extracellular space

  F Lolicato, et al., bioRxiv - Biochemistry 2023

  Quote: ... Liposomes were subjected to 10 freeze/thaw cycles (50°C/liquid nitrogen) and to 21 size-extrusion steps (400 nm pore size; Avanti Polar Lipids mini-extruder). Liposome preparations were analyzed by dynamic light scattering (Wyatt) ...
• Biomolecular condensates modulate membrane lipid packing and hydration

  Agustín Mangiarotti, et al., bioRxiv - Biophysics 2023

  Quote: 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) was purchased from Avanti Polar Lipids (IL, USA). 6-acetyl-2-dimethylaminonaphthalene (ACDAN ...
• Wetting and complex remodeling of membranes by biomolecular condensates

  Agustín Mangiarotti, et al., bioRxiv - Biophysics 2023

  Quote: ... galbeta1-3galnacbeta1-4(neuacalpha2-3)galbeta1-4glcbeta1-1’-cer (GM1) as powder were purchased from Avanti Polar Lipids (IL, USA). The soluble dye 2-(3-diethylamino-6-diethylazaniumylidene-xanthen-9-yl)-5-sulfo-benzenesulfonate (Sulforhodamine B ...
• Phosphatidylethanolamine facilitates mitochondrial pyruvate entry to regulate metabolic flexibility

  Piyarat Siripoksup, et al., bioRxiv - Physiology 2022

  Quote: ... and internal standards (Mouse SPLASH LIPIDOMIX Avanti Polar Lipids 330707 and Cardiolipin Mix I Avanti Polar Lipids LM6003 ...
• Phosphatidylethanolamine facilitates mitochondrial pyruvate entry to regulate metabolic flexibility

  Piyarat Siripoksup, et al., bioRxiv - Physiology 2022

  Quote: ... and internal standards (Mouse SPLASH LIPIDOMIX Avanti Polar Lipids 330707 and Cardiolipin Mix I Avanti Polar Lipids LM6003) were added to isolated skeletal muscle mitochondria isolated mitochondria from C2C12 myotubes or plantaris skeletal muscle ...
• Trans-omic analysis reveals opposite metabolic dysregulation between feeding and fasting in liver associated with obesity

  Yunfan Bai, et al., bioRxiv - Systems Biology 2023

  Quote: ... 10 μL internal standard (IS) A (Mouse SPLASH Lipidomix Mass Spec Standard, Avanti Polar Lipids Inc., Alabaster, AL, USA) containing 1.0 nmol phosphatidylcholine (PC ...