Labshake search
Citations for Covaris :
1 - 15 of 15 citations for Nuclear Protein Extraction since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Genomics 2019Quote: ... 10 mM NaCl, 0.2 % IGEPAL CA-630) and sonication following the standardized nuclear extraction NEXON protocol (Arrigoni et al., 2015) (Covaris E220 sonicator ...
-
bioRxiv - Molecular Biology 2019Quote: ... The nuclear suspension was sonicated using the S220 Focused-ultrasonicator (Covaris) for 8 min (Average Incident Power 14 Watt ...
-
bioRxiv - Physiology 2021Quote: ... the nuclear pellet was resuspended in cold 0.25% SDS Shearing Buffer (Covaris). Chromatin was sheared in 1 ml AFA milliTUBEs (Covaris ...
-
bioRxiv - Microbiology 2021Quote: ... and nuclear extracts were washed in TE 0.1% SDS with protease inhibitors and sonicated using a S220 Focused-ultrasonicator (Covaris). Prior to immunoprecipitation ...
-
bioRxiv - Immunology 2023Quote: ... and nuclear extracts were washed in TE 0.1% SDS with protease inhibitors and sonicated using a S220 Focused-ultrasonicator (Covaris). Prior to immunoprecipitation ...
-
bioRxiv - Genomics 2020Quote: ... Nuclear lysates were quickly spun down for 10 seconds at 2000g and supernatant transferred to sonication tubes (Covaris; product 520130) for shearing ...
-
bioRxiv - Cancer Biology 2023Quote: ... 10mM hydroxyflutamide for 3 or 16 hours and nuclear fractions were sonicated using a waterbath sonicator at 4°C (Covaris). Supernatants were precleared using Protein G DynaBeads (Thermo ...
-
bioRxiv - Genomics 2020Quote: ... The FFPE Kidney and Lung samples used for the nucleic acid extraction optimization were provided by Covaris.
-
bioRxiv - Microbiology 2022Quote: RNA extraction from FFPE tissue was performed with a combination of truXTRAC FFPE total NA Kit (Covaris, Brighton, UK) and the Agencourt RNAdvance Tissue Kit (Beckman Coulter ...
-
bioRxiv - Cancer Biology 2019Quote: ... the samples were treated with RNAse and DNA extracted as per the truXTRAC FFPE DNA extraction protocol (Covaris cat#: 520136). The amount of DNA was quantified using the Qubit® dsDNA HS Assay (Thermo Fisher Scientific).
-
bioRxiv - Plant Biology 2023Quote: ... Young leaves from a single 3-week-old plant were collected for high-molecular-weight DNA extraction followed by shearing to fragments with an average size of 20 kb using g-TUBE (Covaris). The sheared DNA was then used to construct a PacBio SMRT library following the standard SMRT bell construction protocol ...
-
bioRxiv - Systems Biology 2023Quote: ... Afterwards approximately 100 mg of capsule tissue was transferred frozen to a tissue extraction bag (tissueTUBE TT1, extra thick, Covaris Inc) and placed in liquid nitrogen ...
-
bioRxiv - Immunology 2021Quote: ... PBMCs were suspended in an optimized solvent extraction mixture of 9:1 (methanol: chloroform) as described elsewhere in detail75 and extracted with a focused ultrasound system (Covaris E220, Woburn, USA). The extraction solutions were evaporated to dryness for 4 hours in a vacuum concentrator and afterwards pellets resuspended with 45 µL in a 1 mM TSP containing deuterated phosphate buffer ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... For each sample the DNA of pooled female and male flies was extracted using a high salt extraction protocol [58] and sheared with a Covaris S2 device (Covaris, Inc. Woburn, MA, USA). Libraries were prepared using the TruSeq DNA PCR-Free protocol (Illumina ...
-
bioRxiv - Cancer Biology 2023Quote: Myocardium proteins were extracted from cryo-fractured tissue using an automated tissue pulverizer (cyroPREP Tissue Disruption System, Covaris model CP02) prior to the addition of urea lysis buffer and sonication.