Labshake search
Citations for Mirus Bio :
1 - 50 of 760 citations for pVectOZ GFP Transfection control since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2021Quote: Mt1-/-Mt2-/- BMDMϕ were transfected with pCMV6-Ac-GFP control vector or pCMV6-Ac-MT3-GFP vector using the LT1 transfection reagent (Mirus Bio) in RPMI media containing 10% serum without antibiotics as per the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2022Quote: ... Candidate and control vectors were tested for activity by transient transfection with Trans-IT LT1 transfection reagent (Mirus) into murine neuron-like N2A cells followed by Trizol RNA isolation (Thermo ...
-
bioRxiv - Biochemistry 2024Quote: ... Two samples each were treated with Opti-MEM with 2 µg of transfection DNA (either pJG01, or negative control without DNA) along with TransIT-X2 transfection reagent (Mirus Bio) premixed and allowed to incubate 5 minutes at room temperature ...
-
bioRxiv - Systems Biology 2021Quote: ... and grown in complete media for 1 day before co-transfection of the mammalian expression vectors encoding GFP and viral protein of interest using TransIT-2020 transfection reagent (#MIR5404, Mirus). After 24 h ...
-
bioRxiv - Molecular Biology 2021Quote: ... BV2 cells were transfected with GFP plasmid using TransIT transfection reagent (Mirus #MIR5404) following the manufacturer’s protocol ...
-
bioRxiv - Cell Biology 2024Quote: ... Clonal cell lines expressing GFP/biotin ligase fusions were generated using TransIT transfection reagent (Mirus) by co-transfection of 0.6µg/ml expression plasmid with 0.06µg/ml pCoBLAST selection plasmid (Invitrogen) ...
-
bioRxiv - Molecular Biology 2023Quote: ... cells were transfected with the STING-GFP expression constructs using the Trans-IT transfection reagent (Mirus). After transfection ...
-
bioRxiv - Cell Biology 2020Quote: ... Cells were transfected with 5 μg of pACT-GFP-actin using TransIT-Insect transfection reagent (Mirus Bio) and incubated for 2 d at 28°C in Grace’s media with 10% FBS and antibiotics (100 μg/ml penicillin/streptomycin and 0.25 μg/ml Amphotericin B) ...
-
bioRxiv - Cell Biology 2023Quote: ... pSPAX2 and Nb80-GFP and SNAP-β1AR plasmids using as transfection reagent TransIT-Lenti (mir6600, Mirus bio). Lentivirus was produced in DMEM containing 10% FBS and 1% BSA and then concentrated using the Lenti-X concentrator (Takera Bio).
-
bioRxiv - Molecular Biology 2023Quote: ... GFP- and FLAG-tagged proteins under the control of the Actin promoter using TransIT-LT1 reagent (Mirus). 24-40 h after transfection ...
-
bioRxiv - Genomics 2019Quote: ... HEK293 cells were transfected with either 2.5 or 8 ug of the pCMV-Aire-Flag plasmid (or control plasmid) using 7.5 or 32 ul of the TransIT-293 transfection reagent (Mirus). After 48h ...
-
bioRxiv - Molecular Biology 2019Quote: ... plus pMK1221-control or −GR shRNA (2.88 ug shCtrl or shGR, respectively) using TransIT-293 Transfection Reagent (Mirus Bio) as instructed by the manufacturer ...
-
bioRxiv - Immunology 2019Quote: ... and pZIP-mCMV-ZsGreen-shRNA NT Control or mTOR plasmids (2 µg; Transomic) using the TransIT-LT1 transfection reagent (Mirus) as per manufacturer’s protocol ...
-
bioRxiv - Cancer Biology 2021Quote: ... and pMIG-luciferase-IRES-GFP was produced by transfection of 293T cells with TransIT-293 (Mirus Bio, Madison, WI), lentiviral backbone as well as packaging vectors Δ8.9 (gag/pol ...
-
bioRxiv - Microbiology 2019Quote: ... HEK 293 cells were transfected with YF-NS1-GFP using TransIT®-LT1 transfection reagent (Mirus Bio LLC, Belgium), according to the manufacturer’s instructions ...
-
bioRxiv - Genomics 2020Quote: ... or with 1μg of plasmid expressing TTFI-GFP under the full CMV promoter using Transit-X2 transfection reagent (Mirus) in Opti-MEM GlutaMAX reduced-serum medium (Life Technologies) ...
-
bioRxiv - Biophysics 2023Quote: ... STARD11-GFP WT or mutant plasmids were transfected using the TransIT-X2® Transfection Reagent (Mirus Bio, MIR 6000). Cells were fixed in 3% paraformaldehyde for 20 min before staining with antibodies diluted in a PBS solution of 1% BSA and 0.05% saponin ...
-
bioRxiv - Microbiology 2021Quote: ... TK-Renilla luciferase reporter plasmid (as an internal control) and the desired expression vectors or empty vector (EV) using TransIT-LT1 transfection reagent (Mirus Bio), according to the manufacturer’s instruction ...
-
bioRxiv - Microbiology 2020Quote: ... cells were transfected overnight with either a control plasmid (PLX) or TMPRSS2 (PLX-TMPRSS2) via TransIT LT-1 transfection reagent (Mirus Bio) in 100 µL of OptiMEM per well ...
-
bioRxiv - Cell Biology 2020Quote: ... a plasmid containing a cAMP response element binding site driving firefly luciferase was transfected with a control plasmid containing a CMV promotor driving renilla luciferase expression using the TransiT-X2 transfection reagent (Mirus Bio) 48 hours after the siRNA transfection ...
-
bioRxiv - Neuroscience 2022Quote: ... and Fc (a negative control) were generated using HEK293T cells transfected with the corresponding expression vectors using TransIT-PRO Transfection Reagent (Cat# MIR5740, Mirus Bio) and maintained in serum-free AIM V synthetic medium (Cat# 12055083 ...
-
bioRxiv - Microbiology 2023Quote: ... HeLa cells were co-transfected on a six-well plate with a 3:1 mass ratio mix of pM1-2C and pArf1-EGFP plasmids (or a plasmid expressing EGFP instead of Arf-EGFP in controls) using Mirus 2020 transfection reagent (Mirus Bio). Infected cells were processed at 6 ...
-
bioRxiv - Immunology 2020Quote: ... Cells were infected with AD169r-GFP at an MOI of 1.0 or transfected with gB-encoding mRNA using the TransIT mRNA transfection kit (Mirus Biosciencies), then incubated a further 48 hours at 37°C ...
-
bioRxiv - Molecular Biology 2021Quote: ... HEK293T-GFP cells were transiently transfected with wild-type and mutant spike plasmids using TransIT-X2 transfection reagent (Mirus #MIR 6000) according to the manufacturer’s instructions ...
-
bioRxiv - Biophysics 2022Quote: CHO-GFP-CCR5 cell lines were generated by transfecting CHO-K1 with a pCDNA3.1 Hygro-GFP-CCR5 construct using the TransIT-X2 transfection reagent from Mirus (MIR 6003). CCR5 expression was maintained by culturing cells in the presence of 400 µg/ml hygromycin and GFP-CCR5 expressing cells were isolated for single-cell cloning through the process of serial dilution cloning ...
-
bioRxiv - Cell Biology 2022Quote: ... cells in each well were transfected with a plasmid encoding GFP-2A-POI (protein of interest) using TransIT-2020 transfection reagent (Mirus #5404) as follows ...
-
bioRxiv - Neuroscience 2020Quote: ... Transfections were performed with TransIT-LT1 Transfection Reagent (Mirus) according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2022Quote: ... Transfections were performed using TransIT-LT1 transfection reagent (Mirus), following the protocol described for the generation of recombinant fluorescently marked RVFV-35/74 variants.
-
bioRxiv - Microbiology 2022Quote: ... Transfections were performed using TransIT-LT1 transfection reagent (Mirus), with slight modifications to the manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2020Quote: ... Cells were then transfected with a Cas9-GFP-expressing plasmid (pSpCas9(BB)-2A-GFP) and the gRNA containing STAgR-Neo plasmid using the TransIT-X2 transfection reagent (Mirus Bio LLC) according to manufacturer’s instructions ...
-
Rag GTPases and Phosphatidylinositol 3-Phosphate Mediate Recruitment of the AP-5/SPG11/SPG15 ComplexbioRxiv - Cell Biology 2020Quote: ... HeLa cells stably expressing GFP-FYVE(SPG15) were created using retrovirus made in HEK 293ET cells transfected using TransIT-293 Transfection Reagent (Mirus Bio LLC), according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2022Quote: ... Transfection was done using the TransIT-LT1 Transfection Reagent (Mirus). Two solutions were prepared for each well ...
-
bioRxiv - Neuroscience 2019Quote: ... Transfections were performed using TransIT LT1 transfection reagent (Mirus Bio) according to the manufacturer’s protocol ...
-
bioRxiv - Cell Biology 2023Quote: ... Transfections were performed using Transit-LT1 transfection reagent (Mirus Bio) at a 1:3 DNA to transfection reagent ratio ...
-
bioRxiv - Cell Biology 2023Quote: ... All transfections were performed using TransIT-2020 transfection reagent (Mirus) according to the supplier’s manual ...
-
bioRxiv - Cell Biology 2022Quote: Monoclonal and polyclonal hIPSC-dCas9 lines were transfected with pCS2-iREP-GFP-PGK-BFP-U6-gRNA-irep or pCS2-iREP-GFP-PGK-BFP-U6-gRNA-mock vectors as replicates in 12 well plates using TransIT®-LT1 Transfection Reagent (Mirus Bio, MIR2300) according to manufacturer’s instructions and as detailed above ...
-
bioRxiv - Biophysics 2020Quote: ... The transfection was performed using the TransIT-LT1 transfection reagent (Mirus) according to the manufacturer’s protocol.
-
bioRxiv - Microbiology 2019Quote: ... Transfections were performed with 6.25 μL TransIT LT1 transfection reagent (Mirus) in 250 μL Opti-MEM (Gibco ...
-
bioRxiv - Neuroscience 2021Quote: ... RNA transfections were performed with TransIT-mRNA Transfection Kit from Mirus Bio ...
-
bioRxiv - Microbiology 2020Quote: Transfections were performed using TransIT-LT1 Transfection Reagent (Mirus, MIR 2305) according to the manufacturer’s protocol ...
-
bioRxiv - Microbiology 2020Quote: ... Transfections were performed with the TransIT-LT1 transfection reagent (Mirus, #MIR2304) at a reagent:plasmid DNA ratio of 3:1.
-
bioRxiv - Molecular Biology 2022Quote: ... Transfection was performed using the Transit-LT1 transfection reagent from Mirus Bio (distributed by Sopachem ...
-
bioRxiv - Microbiology 2023Quote: ... siRNA transfections were performed with TransIT-X2 Transfection Kit (Mirus Bio) following manufacturer’s instructions ...
-
bioRxiv - Biochemistry 2024Quote: ... they were transfected with 1800 ng of plasmid containing either N- or C-terminal tagged GFP-AMPKγ3 using TransITx2 transfection reagent (Mirus Bio, cat no. MIR 6000) and then lysed 48 hours later (lysis buffer – 50 mM Tris ...
-
bioRxiv - Molecular Biology 2022Quote: ... co-transfection in 293GP cells with TransIT-LT1 Transfection Reagent (Mirus, 2300). Two days later ...
-
bioRxiv - Cell Biology 2021Quote: ... plasmids by transient transfection using the TransIT®-LT1 Transfection Reagent (Mirus). Transfections used 0.5 – 1 μg plasmid DNA per coverslip and were carried out according to the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2021Quote: ... Reverse transfection by using TransIT-Jurkat transfection reagent (Mirus, Cat.# MIR 2124) was done after cells plating ...
-
bioRxiv - Cancer Biology 2023Quote: ... Transfections were performed using TransIT™-LT1 transfection reagent (#MIR2304, Mirus Bio) using 300 ng of the CRISPR plasmid and 100 ng of each sgRNA plasmid per well of a 96 well plate ...
-
β-catenin programs a tissue-specific epigenetic vulnerability in aggressive adrenocortical carcinomabioRxiv - Cancer Biology 2022Quote: ... and transfection reagent (Mirus) prepared according to manufacturer instructions ...
-
bioRxiv - Immunology 2022Quote: ... RNA PAMP transfections were done using the TransIT-mRNA Transfection kit (Mirus Bio). The siRNA transfections were done using Lipofectamine RNAiMax (Invitrogen) ...