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Citations for Mirus Bio :
1 - 50 of 87 citations for SARS CoV 2 Spike Glycoprotein S2 aa 800 1000 His Tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... cells were transfected with a plasmid encoding for SARS-CoV-2 S-glycoprotein (YP_009724390.1) harboring a C-terminal 19 aa truncation using TransIT-Lenti (Mirus Bio) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... transfected with 44 μg plasmid encoding SARS-CoV-2 Spike (pCG1-SARS-2-S, Wuhan Hu-1) using Transit LT-1 (Mirus). The next day ...
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bioRxiv - Microbiology 2021Quote: ... Cells were transfected with 250 ng of a pCDNA3.1(+) vector encoding for the SARS-CoV-2 spike protein using the TransIT®-LT1 Transfection Reagent (Mirus Bio). Six hours post transfection (p.t.) ...
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bioRxiv - Immunology 2020Quote: HEK293T cells were transiently transfected with mRNA encoding SARS-CoV-2 WT S or S-2P protein using a TranIT mRNA transfection kit (Mirus). After 24 hr ...
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bioRxiv - Microbiology 2024Quote: ... 80% confluent monolayers of Vero E6 cells in 12-well plates were transfected with 1.0 μg per well of infectious SARS-CoV-2 BAC DNA or its mutated derivatives using TransIT®-LT1 transfection reagent according to manufacturer’s instructions (Mirus Bio). At 48 h post transfection ...
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bioRxiv - Microbiology 2021Quote: ... or 0.9 µg of pCDNA3.1-SARS-Spike using the TransIT®-LT1 Transfection Reagent (Mirus) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... the cells were transfected with a SARS-CoV-2 orf-expressing plasmid and the packaging plasmids using TransIT-LT1 transfection reagent (Mirus Bio, Madison, WI) according to the provider’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... 293T cells on 96-well plate were co-transfected with a set of pGS-AVLQS and pSARS-CoV-2 Mpro or a set of pGS-RLKGG and pSARS-CoV-2 PLpro using TranIT-LT1 (Mirus Bio) in the presence of serial diluted S-217622 ...
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bioRxiv - Microbiology 2022Quote: ... 293T cells were transfected with arenavirus glycoprotein expression plasmids using TransIT-LT1 (Mirus Bio). Cells were transduced the following day with VSV-ΔG-GFP (Kerafast)(45 ...
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bioRxiv - Molecular Biology 2019Quote: ... S2 cells were transfected with T ransIT-LT1 (Mirus). 24-48h post transfection cells were harvested and lysed in lysis buffer (20 mM Tris-HCl at pH 7.4 ...
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bioRxiv - Molecular Biology 2020Quote: ... S2 cells were transfected with Bio TransIT-2020 Reagent (Mirus), according to the manufacturer instructions (2μg of total DNA) ...
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bioRxiv - Molecular Biology 2023Quote: ... The cells were transfected with 15 μg of a glycoprotein expressing vector using TransIT®-LT1 (Mirus). 24 h post-transfection ...
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bioRxiv - Immunology 2021Quote: ... 1% PenStrep) were transfected with 25 μg of plasmid encoding for the corresponding S glycoprotein using TransIT-Lenti (Mirus) as transfectant reagent ...
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bioRxiv - Genetics 2020Quote: ... transient transfections were conducted on S2 cells using the TransIT-2020 reagent (Mirus), and live imaging was performed 72 hours later ...
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bioRxiv - Immunology 2021Quote: ... Retroviral particles were produced by transfection of Platinum E (PLAT-E) cells with the TransIT-LT1 transfection reagent (Mirus) in Opti-MEM I reduced serum medium ...
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bioRxiv - Biophysics 2022Quote: ... with 600 – 1000 ng DNA and 2 μL TransIT-LT1 transfection reagents (Mirus Bio Corporation) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2019Quote: ... plasmid vectors coding the glycoproteins were transfected into BSR-T7 cells using the TransIT®-LT1 Transfection Reagent (Mirus Bio, MIR 2300). At 16 h post-transfection ...
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bioRxiv - Cell Biology 2023Quote: ... The construct was transfected into low pass S2 cells using TransIT Insect transfection reagent (Mirus) and stable cells carrying indels for this locus were selected for with 5 μg/ ml puromycin for 3 weeks ...
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bioRxiv - Cell Biology 2020Quote: ... GFP-tagged E-cadherin or with constructs encoding E-cadherin and TNFα RUSH system using TransIT-LT1 Reagent (Mirus, MIR 2305) according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2021Quote: ... Confirmed plasmids were transformed into S2 cells using TransIT Insect Transfection Reagent (Mirus Bio, Madison, WI, USA). Transformed cells were grown for 48 h before use in experiments.
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bioRxiv - Cell Biology 2022Quote: ... 1ug of spike plasmid for each well was transfected into cells using transfection reagent (Mirus, TransIT-LT1) with adding DMSO or antiviral compounds for 24 hours ...
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bioRxiv - Evolutionary Biology 2023Quote: ... pLAS2w.Fluc.Ppuro and pcDNA3.1-SCoV2-S(B.1.1.7) related spike expression constructs using TransITR-LT1 transfection reagent (Mirus). Culture media were refreshed at 16 hr and harvested at 48 hr and 72hr post-transfection ...
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bioRxiv - Microbiology 2021Quote: ... 8×106 cells were suspended in 800 μl Ingenio® Electroporation Solution (Mirus Bio, Madison, WI) and mixed with 10 μg RNA in a 4-mm cuvette ...
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bioRxiv - Molecular Biology 2023Quote: ... pAC5.1 vectors of CLIP-CRYΔ and TIM- SNAP-HA-ALFA were delivered into S2 cells using the TransIT-Insect Transfection Reagent (Cat# MIR6100, Mirus) following a modified version of the manufacturer’s instruction ...
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bioRxiv - Biochemistry 2019Quote: ... The plasmid was co-transfected into S2 insect cells with 10:1 molar excess of pCoBlast using TransIT-Insect transfection reagent (Mirus Bio). Stable cell lines were selected with 100 µg/mL blasticidin (Cambridge Bioscience) ...
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bioRxiv - Microbiology 2021Quote: ... were seeded in 10-cm2 dishes at a density of 5e6 cells per dish and the following day transfected with 10 μg of WT or B.1.617.2 spike expression plasmid with TransIT-Lenti (Mirus, 6600) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... 293T cells were transfected with a plasmid expressing SARS-CoV-2 Spike (kindly provided by Markus Hoffmann and Stefan Pöhlmann Hoffmann et al., 2020 PMID: 32142651) using TransIT-X2 (Mirus) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... and a circular Rpl13a repair template into cultured Drosophila S2 cells using TransIT Insect Transfection Reagent (Mirus Bio LLC, Madison, WI, USA). Gene editing efficiency of the different sgRNAs was evaluated five days after transfection by cellular fluorescence and genotyping using a primer outside of the homology arm and the other inside the inserted PQR sequence.
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bioRxiv - Immunology 2022Quote: ... were seeded in 15-cm2 dishes at a density of 10L×L106 cells per dish and the following day were transfected with 25Lµg of spike expression plasmid with TransIT-Lenti (Mirus, 6600) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... HEK293T-GFP cells were transiently transfected with wild-type and mutant spike plasmids using TransIT-X2 transfection reagent (Mirus #MIR 6000) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... were seeded in 10-cm dishes at a density of 1e5 cells/cm2 and the following day transfected with 5 μg of spike expression plasmid with TransIT-Lenti (Mirus, 6600) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... were seeded in 10-cm dishes at a density of 1e5 cells/cm2 and the following day transfected with 5 μg of spike expression plasmid with TransIT-Lenti (Mirus, 6600) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... were seeded in 15-cm2 dishes at a density of 10e6 cells per dish and the following day transfected with 25 μg of spike expression plasmid with TransIT-Lenti (Mirus, 6600) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... were seeded in 10-cm2 dishes at a density of 5e6 cells per dish c and the following day transfected with 10 μg of spike expression plasmid with TransIT-Lenti (Mirus, 6600) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... were seeded in 15-cm2 dishes at a density of 10 × 106 cells per dish and the following day were transfected with 25 µg of spike expression plasmid with TransIT-Lenti (Mirus, 6600) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: HEK293T-EGFP cells (donor cell) were transiently transfected with wild-type and mutant spike plasmids using TransIT-X2 transfection reagent (Mirus #MIR 6000). HEK293T-ACE2 and HEK293T (as a negative control ...
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bioRxiv - Cell Biology 2022Quote: HeLa cells were transiently transfected with pLVX-EF1alpha-nCoV2019 2xStrep- tagged SARS-CoV2 ORF constructs [47] using TransIT-HeLa Monster (Mirus Bio). After 48 hours transfection ...
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bioRxiv - Microbiology 2021Quote: ... Wild-type/mutant MeV-M bearing a FLAG-tag and NiV-M bearing an HA-tag were cloned into pCMV and pcDNA3.1 respectively and transfected into cells using TrasnIT-LT1 transfection reagent (Mirus). VLPs were harvested 24 hours post-transfection ...
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bioRxiv - Immunology 2023Quote: Retrovirus was generated by transfecting the Plat-E packaging cell line with 2.5 μg DNA and 7.5 μl TransIT-X2 Reagent (Mirus, MIR 600). B cells were isolated and cultured in complate 1640 RPMI medium before transduction ...
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bioRxiv - Biophysics 2019Quote: ... constructs containing an amber stop codon (TAG) at amino acid position 1353 or 1397 (1353stop or 1397stop) using TransIT transfection reagent (Mirus Bio LLC; Madison, WI) in a ratio of 3 µL of TransIT per µg of total DNA ...
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bioRxiv - Microbiology 2022Quote: ... or 2 µg of pPol1II-HA and 2 µg of pPol1II-NA) using TransIT-293 (Mirus). Virus supernatant was harvested 72 hours post-transfection and filtered through 0.22 µm filters ...
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bioRxiv - Microbiology 2022Quote: ... or 2:2:1 (33) with TransIT®-LT1 transfection reagent (Mirus Bio LLC, Madison, WI, USA) and the plasmids ...
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bioRxiv - Molecular Biology 2023Quote: ... 2 µL of Boost reagent (Mirus Bio) and 2 µL of TransIT mRNA reagent (Mirus Bio) ...
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bioRxiv - Molecular Biology 2023Quote: ... and 2 µL of TransIT mRNA reagent (Mirus Bio). Transfection reactions were incubated at room temperature for 3 minutes prior to drop-wise addition into each well ...
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bioRxiv - Microbiology 2021Quote: ... Cells were transferred into cuvettes (2 mm gap, Mirus, MIR50121) and subjected for electroporation using Nucleofector2b (Lonza) ...
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bioRxiv - Neuroscience 2021Quote: ... using 2 μL of TransIT-Insect transfection reagent (Mirus Bio Cat #6104). Medium was replaced with serum-containing medium 5 hours after transfection ...
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bioRxiv - Biophysics 2023Quote: ... The cells were transfected at a confluency of ∼80% with 600-1000 ng of plasmid and 6 uL of TransIT293T (Mirus) transfection reagent ...
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bioRxiv - Biophysics 2022Quote: ... with ∼ 600ng DNA and 2 µL TransIT-LT1 transfection reagents (Mirus Bio Corporation) following the manufacturer’s protocol ...
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bioRxiv - Biophysics 2023Quote: ... 2 µg of plasmid DNA was transfected per dish using Transit 293T (Mirus) following the manufacturer’s instructions and measured at least 48 h later.
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bioRxiv - Immunology 2022Quote: ... 250 μl of OptiMEM media and supplemented with 2 μl TransIT-2020 Transfection Reagent (Mirus, MIR5400). The transfection mix was incubated 20 min at room temperature and then added to the cell culture for 24 h ...