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Citations for Mirus Bio :
1 - 50 of 322 citations for Human PIGV shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... 38HK were transiently transfected with lentiviral shRNA plasmids using TransIT-Keratinocytes Transfection Reagent (Mirus) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2020Quote: ... Lentiviral particles were produced by co-transfection of shRNA plasmids with psPAX and pMDG.2 in 293T cells using the Trans-IT LT-1 transfection reagent (Mirus). DM cells were infected by shRNA lentivirus twice via spinoculation in the presence of 5 μg/mL polybrene (Sigma-Aldrich) ...
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bioRxiv - Immunology 2019Quote: ... and pZIP-mCMV-ZsGreen-shRNA NT Control or mTOR plasmids (2 µg; Transomic) using the TransIT-LT1 transfection reagent (Mirus) as per manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: Human Alveolus Chips were transfected with plasmid DNA using the TransIT-X2 reagent (Mirus Bio). 3 ug of plasmid DNA and 6 ul TransIT-X2 reagent were constituted in 300 ul OPT-MEM before added to 3 ml chip flow medium ...
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bioRxiv - Cell Biology 2023Quote: ... shRNA lentivirus was prepared by transfecting 293T cells using TransIT-293 (Mirus) using a standard protocol from Addgene.
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bioRxiv - Cell Biology 2022Quote: ... or non-Targeting shRNA vectors using Mirus Trans-IT TKO Transfection reagent (Mirus). 621-101 cells were transduced with lentiviruses for 48 hr and then selected against puromycin ...
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bioRxiv - Cell Biology 2023Quote: ... Cells expressing shRNA were transiently transfected using TransIT-LT1 reagent (Mirus, Cat# MIR-2300) in Opti-MEM (ThermoFisher ...
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bioRxiv - Cancer Biology 2022Quote: ... or non-Targeting shRNA vectors pLKO.1 using Mirus Trans-IT TKO Transfection reagent (Mirus). 621-101 cells were transduced with lentiviruses for 48 hours and then selected against puromycin ...
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bioRxiv - Genetics 2021Quote: Plasmid expressing HA-tagged human WFS1 was transfected into HeLa cells using TransIT-X2® Dynamic Delivery System (Mirus Bio; MIR 6000). After 24 hours ...
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bioRxiv - Neuroscience 2019Quote: ... VSV-G pseudotyped lentiviral vectors were produced by transfection of human embryonic kidney cells (HEK293FT) with third-generation lentivirus plasmids using lipofection (Mirus TransIT®-293). Supernatant was collected 48 h after transfection and concentrated using ultrafiltration (Centricon Plus-20 PLGC centrifuge filter units).
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bioRxiv - Molecular Biology 2019Quote: ... plus pMK1221-control or −GR shRNA (2.88 ug shCtrl or shGR, respectively) using TransIT-293 Transfection Reagent (Mirus Bio) as instructed by the manufacturer ...
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bioRxiv - Immunology 2020Quote: ... 293T HEK cells were plated in 10cm plates one day prior to transfection and individually transfected with 10ug of each shRNA retroviral construct and 5ug pCL-Eco using TransIT-LT1 (Mirus). Retroviral supernatant was collected and pooled at 48 and 72 hours post-transfection ...
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bioRxiv - Cell Biology 2022Quote: ... the packaging plasmid psPAX2 and the envelope plasmid pVsVg using TransIT- Lenti transfection reagent (Mirus). The transfection mix was incubated for 20 min at RT ...
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bioRxiv - Immunology 2021Quote: ... cells were transfected with pCMV6-XL5 empty plasmid or S100A7 plasmid using TransIT-X2 Dynamic Delivery System (Mirus).
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bioRxiv - Microbiology 2021Quote: ... Plasmids and TransIT-293 transfection reagent (Mirus) were mixed at a ratio of 1 µg DNA ...
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bioRxiv - Genomics 2019Quote: ... HEK293 cells were transfected with either 2.5 or 8 ug of the pCMV-Aire-Flag plasmid (or control plasmid) using 7.5 or 32 ul of the TransIT-293 transfection reagent (Mirus). After 48h ...
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bioRxiv - Cancer Biology 2019Quote: ... in combination with 3μg psPAX2 (packaging plasmid) and 2μg pMD2.G (envelope plasmid) with 30μL TransIT-LT1 transfection reagent (Mirus, MIR 2300). For T-ALL cell infection ...
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bioRxiv - Microbiology 2022Quote: ... with pRL-EMCV-FL reporter plasmid and the indicated pCAGGS-based nsp1 expression plasmids using TransIT-LT1 reagent (Mirus). At 24 hours post-transfection ...
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bioRxiv - Microbiology 2022Quote: ... Cells were transfected with 7.7 µg of a plasmid (split evenly by mass between the plasmids indicated) using TransIT-LT1 (Mirus) and after 18 h the cells were mock-treated (refreshed media ...
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bioRxiv - Genetics 2023Quote: ... Transfections containing 70 ng of ABE expression plasmid and 30 ng sgRNA expression plasmid mixed with 0.72 μL of TransIT-X2 (Mirus) in a total volume of 15 μL Opti-MEM (Thermo Fisher Scientific) ...
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bioRxiv - Biophysics 2023Quote: ... Transfections were performed 20 hours following seeding of 2x104 HEK 293T cells per well in 96-well plates and contained 70 ng of ABE8e plasmid and 30 ng of sgRNA expression plasmid mixed with 0.3 µL of TransIT-X2 (Mirus) in a total volume of 15 µL Opti-MEM (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2020Quote: ... The following day they were transfected with 6 μg Gag plasmid + 6 μg vector plasmid + 3 μg pCMV-Rev (to support nuclear export of vector RNA) using Transit-293 (Mirus) following the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2021Quote: ... lentiviral particles were generated by transient transfection of the lentiviral packaging Lenti-X 293T cells (Takahara) with the TCR encoding plasmids and packaging plasmids (VSVg and PSPAX2) 25 using Transit LT-1 (Mirus). Lentiviral supernatant was harvested 2 days later ...
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bioRxiv - Cell Biology 2021Quote: ... cells were transfected with the pFhSynW2 TauRD (P301L/V337M)-EYFP expression plasmid and the packaging plasmids psPAX2 and pVsVg using TransIT-Lenti transfection reagent (Mirus). The transfection mix was incubated for 20 min at room temperature (RT ...
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bioRxiv - Molecular Biology 2023Quote: ... HEK T-REx wild type or SCAPER KO cells were seeded in 12-well plates and transfected the next day with pcDNA5/FRT/TO rescue plasmids and a puromycin-resistance conferring plasmid (MXS-CMV-PuroR) using TransIT-293 (Mirus). 24 hours after transfection ...
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bioRxiv - Cell Biology 2022Quote: Lentivirus was generated by co-transfection of HEK293T cells with a desired transfer plasmid and two packaging plasmids (psPAX2 and Δ8.9) using the TransIT-293 transfection reagent (Mirus, USA). 48 h post transfection ...
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bioRxiv - Microbiology 2019Quote: ... The plasmid DNA and transfection reagent TranSIT (Mirus) were mixed in 100 μL Opti-MEM without phenol red (Life Technologies ...
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bioRxiv - Cell Biology 2022Quote: ... Plasmid DNA was transfected using TransIT (Mirus Bio) by adding 1 µl of the reagent and 1 µg of DNA per 48-plate well ...
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bioRxiv - Molecular Biology 2019Quote: ... a 10 cm dish of cells was transfected with 9 µg of Flp-Recombinase (plasmid pOG44) and 1 µg of a specific pcDNA5/FRT plasmid using TransIT-293 transfection reagent (Mirus, MIR2705). 48 hours after transfection ...
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bioRxiv - Microbiology 2023Quote: ... HeLa cells were co-transfected on a six-well plate with a 3:1 mass ratio mix of pM1-2C and pArf1-EGFP plasmids (or a plasmid expressing EGFP instead of Arf-EGFP in controls) using Mirus 2020 transfection reagent (Mirus Bio). Infected cells were processed at 6 ...
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bioRxiv - Immunology 2019Quote: Lentivirus was produced by transfecting lentiviral plasmids and 2nd generation packaging/polymerase plasmids into 293T cells using TransIT-LT1 Reagent (Mirus Bio LLC). Virus-containing supernatants were harvested 72h later ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were then transfected with a Cas9-GFP-expressing plasmid (pSpCas9(BB)-2A-GFP) and the gRNA containing STAgR-Neo plasmid using the TransIT-X2 transfection reagent (Mirus Bio LLC) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: Lentivirus is produced in HEK293t cells by transfection of lentiviral plasmid and packaging plasmids pMD2.G and psPAX2 using TransIT-VirusGEN® Transfection Reagent (Mirus, MIR6705). After two days of transfection ...
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bioRxiv - Cell Biology 2019Quote: ... Plasmid transfections were done with TransIT-LT1 (Mirus Bio) per the manufacturer’s instructions ...
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bioRxiv - Genetics 2021Quote: ... Plasmid transfections were done with TransIT-LT1 (Mirus Bio) per the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2020Quote: ... Plasmid transfections were conducted using Trans-IT LT1 (Mirus) transfection reagent following the manufacturer instruction ...
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bioRxiv - Cell Biology 2021Quote: ... Plasmid transfections were done with TransIT-LT1 (Mirus Bio) per the manufacturer’s instructions.
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bioRxiv - Cell Biology 2019Quote: ... Plasmids were transfected using TransIT-X2 (Mirus Bio LLC) (8) ...
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bioRxiv - Immunology 2021Quote: ... Plasmids were then transfected using TransIT-X2 (Mirus Bio). At 24h post transfection ...
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bioRxiv - Plant Biology 2022Quote: ... Plasmid transfections were done with TransIT-LT1 (Mirus Bio) per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... indicated plasmids were transfected using LT-1 Reagent (Mirus) and cells were then fixed with 5% methanol-free formaldehyde or treated with IFN or TNF-alpha before fixation ...
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bioRxiv - Cell Biology 2023Quote: ... Plasmids were transiently overexpressed using TransIT-LT1 (Mirus, MIR2306) according to the instructions of the manufacturer ...
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bioRxiv - Cell Biology 2021Quote: ... the cells were transfected with a SARS-CoV-2 orf-expressing plasmid and the packaging plasmids using TransIT-LT1 transfection reagent (Mirus Bio, Madison, WI) according to the provider’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... were co-transfected with EcoPack packaging plasmid and the MSCV-IRES-GFP (Empty backbone or MycWT or MycMUT) retroviral plasmids using TransIT®-LT1 (Mirus, MIR 2300) according to manufacturer instructions.
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bioRxiv - Cell Biology 2020Quote: ... SV40 plasmid transfections were conducted using Trans-IT LT1 (Mirus) transfection reagent following the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2019Quote: ... plasmids were transfected into cells using TransIT-LT1 reagent (Mirus) 16–18 h before the assay (0.2 μg of plasmid ...
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bioRxiv - Bioengineering 2022Quote: ... DNA plasmid was labelled with Label IT® (Mirus, USA). Reagents used for immunofluorescence are listed in Supp ...
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bioRxiv - Molecular Biology 2023Quote: ... and AAV transfer plasmids using TransIT-VirusGEN Transfection reagent (Mirus). The next day ...
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bioRxiv - Microbiology 2023Quote: ... pCAGGS-HA plasmids were transfected using 1µL TransIT 2020 (Mirus) per 100µg plasmid and incubated overnight ...
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bioRxiv - Microbiology 2023Quote: ... pCAGGS-HA plasmids were transfected using 1µL TransIT 2020 (Mirus) per 100µg plasmid and maintained at cell culture conditions overnight ...