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Citations for Mirus Bio :
1 - 50 of 70 citations for G CSF Mouse CHO since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... CHO-S cells were transfected with VHH-Fc containing construct using the CHO Gro System (Mirus Bio, WI, USA), according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were transfected using the TransIT-CHO Transfection Kit (Mirus Bio LLC) (CHO-K1 ...
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bioRxiv - Developmental Biology 2019Quote: ... Cells were grown to 70% confluence and transfected with TransIT-CHO Transfection Kit (Mirus Bio LLC, Madison, WI). Transfected cells were split the following day and grown in Ibitreat 15 u-Slide 8 well slides (Ibidi ...
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bioRxiv - Immunology 2021Quote: ... The obtained recombinant vector was transfected into CHO-S cells using CHOgro High Yield Expression System (Mirus Bio, USA). The culture supernatant containing the RBD protein was harvested after 10 days ...
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bioRxiv - Biophysics 2022Quote: CHO-GFP-CCR5 cell lines were generated by transfecting CHO-K1 with a pCDNA3.1 Hygro-GFP-CCR5 construct using the TransIT-X2 transfection reagent from Mirus (MIR 6003). CCR5 expression was maintained by culturing cells in the presence of 400 µg/ml hygromycin and GFP-CCR5 expressing cells were isolated for single-cell cloning through the process of serial dilution cloning ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Transfection of cells seeded in a T25 flask at a confluency of ≥80% was performed using TransIT®-CHO Transfection Kit (MIR 2174, Mirus Bio), in accordance with the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... and 2μg pMD2.G using TransIT (Mirus). Viral supernatant was collected 48 and 72h after transfection ...
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bioRxiv - Cancer Biology 2021Quote: ... pCMV-deltaR8.91 and pMD2.G-VSV-G into HEK293T cells using the TransIT-LT1 Transfection Reagent (Mirus Bio LLC). Virus-containing supernatants were collected 48 hr after transfection ...
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bioRxiv - Cancer Biology 2022Quote: All lentiviruses were produced by co-transfection of HEK 293T/17 cells with plasmid DNA for the respective lentiviral vector and the packaging components psPAX2 and pMD2.G at a 5:3:2 mass ratio (vector:psPAX2:pMD2.G) using TransIT-LT1 (Mirus MIR 2300). Lentiviral supernatants were collected at 48 and 72 hours post-transfection ...
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bioRxiv - Molecular Biology 2023Quote: ... and rev genes and pMD2.G encoding the VSV-G envelope protein using the TransIT-Lenti transfection reagent (Mirus, MIR 6650). The virus was harvested after 48 hours and filtered through a 0.45 μm filter ...
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bioRxiv - Cancer Biology 2023Quote: All lentiviruses were produced by co-transfection of HEK 293T/17 cells with plasmid DNA for the respective lentiviral vector and the packaging components psPAX2 and pMD2.G at a 5:3:2 mass ratio (vector:psPAX2:pMD2.G) using TransIT-LT1 (Mirus MIR 2300). Lentiviral supernatants were collected at 48 and 72 hours post-transfection ...
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bioRxiv - Cancer Biology 2019Quote: ... and envelope (VSV-G) using TransIT-LT1 (Mirus Bio). Supernatant was collected at 48 and 72 hours and applied to target cells with 8 μg/mL polybrene (EMD Millipore ...
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bioRxiv - Genomics 2023Quote: ... and pMD2.g (Addgen #122259) using TransIT-LT1 (Mirus) according to manufacturer instructions ...
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bioRxiv - Cell Biology 2023Quote: ... and pMD2.g using TransIT-LT1 transfection reagent (Mirus) at a 3:1 ratio ...
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bioRxiv - Molecular Biology 2021Quote: The pBR43IeG-nef+-iRFP670 vector was transfected either by itself or with VSV-G envelop plasmid (PMD2.G) into Lenti-X™ cells using Transit-X2 (Mirus Bio, LLC) to make XR-4 tropic or VSV-G pseudotyped HIV-iRFP virus respectively ...
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bioRxiv - Cancer Biology 2022Quote: ... and VSV-G envelope vector mixed with TransIT-293 (Mirus). Virus-containing supernatant was collected 48 ...
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bioRxiv - Cancer Biology 2022Quote: ... and VSV-G envelope vector mixed with TransIT-293 (Mirus). Virus-containing supernatant was collected 36 ...
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bioRxiv - Genomics 2019Quote: ... pMD2.G with TransIT-293 transfection reagent (Mirus, MIR 2706). The following day ...
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bioRxiv - Molecular Biology 2023Quote: ... and VSV-G using the TransIT-LT1 Transfection Reagent (Mirus). Viral supernatants were collected ...
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bioRxiv - Cancer Biology 2023Quote: ... psPAX2 and pMD2.G packaging constructs using TransIT-VirusGEN (Mirus). Supernatants containing high titer lentiviruses were collected at 48 and 72 hr after transfection and were filtered through a 0.45 µm cellulose acetate filters before use ...
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bioRxiv - Cell Biology 2019Quote: ... psPAX2 and pMD2.g using TransIT-LT1 (Mirus, cat. MIR 2304). All transductions were done at a multiplicity of infection (MOI ...
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bioRxiv - Cell Biology 2023Quote: ... and pCG-VSV-G using TransIT-LT-1 transfection reagent (Mirus). Subsequently ...
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bioRxiv - Genomics 2023Quote: ... and VSV-G envelope protein using TransIT®-LT1 Transfection Reagent (Mirus) with ViralBoost Reagent (Alstem ...
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bioRxiv - Biophysics 2022Quote: ... VSV-G and pAdVAntage packaging plasmids into 293FT cells using TransIT-LTI (Mirus). At 48 h post-transfection ...
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bioRxiv - Biophysics 2023Quote: ... and envelope vector (VSV-G) into HEK293T cells using TransIT-LT1 (Mirus Bio). Supernatant post-48/72h transfection was collected for transduction in target cells supplemented with 8 µg/mL polybrene (EMD Millipore) ...
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bioRxiv - Cancer Biology 2021Quote: ... d8.9 packaging vector and VSV-G envelope vector mixed with TransIT-293 (Mirus Bio). Virus containing supernatant was collected at 36 ...
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bioRxiv - Cancer Biology 2019Quote: ... 7.5 µg of psPAX2 and 2.5 µg pMD2.G vectors using TransIT-Lenti (Mirus) following manufacturer’s recommendations ...
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bioRxiv - Immunology 2022Quote: ... 0.4µg pCMV-VSV-G and 1.6µg of viral genomic DNA with TransIT-293 (Mirus) and left O/N ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were transfected with 1.5µg of the pHR plasmid along with two plasmids containing the lentiviral packaging proteins (0.167µg of pMD2.G and 1.3µg of p8.91) with TransIT-293 (Mirus Bio). After 2-3 days of transfection ...
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bioRxiv - Cancer Biology 2020Quote: ... pCMVΔR8.91 and pMD2VSV-G envelope plasmids into HEK293T cells using TransIT-LTI transfection reagent (Mirus Bio). Virus particles collected at 48 hours post-transfection were passed through a 0.45 μm filter and added to SVOG3e cells at a M.O.I ...
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bioRxiv - Cancer Biology 2019Quote: ... and VSV-G vectors at a 4:2:1 ratio with Mirus TransIT LT1 (Mirus Bio, LLC). Virus-containing supernatant was collected 48 and 72h after transfection and filtered through 0.45mm filters before concentration by ultracentrifugation (25,000 RPM for 2 hours with low decel) ...
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bioRxiv - Cell Biology 2022Quote: ... HEK293T cells were co-transfected with a lentiviral sgRNA expression plasmid and the packaging vectors pCMVΔ8.91 and pMD VSV-G (1:0.7:0.3 mix) using TransIT-293 (Mirus Bio). HEK293T media was collected 48 h post-transfection ...
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bioRxiv - Cell Biology 2019Quote: ... 8 µg pCMV-dR8.91 and 1 µg pMD2.G packaging plasmids using 48 µl TransIT-LT1 (Mirus). Plasmid DNA was mixed with transfection reagent in Opti-MEM (Thermo Fisher Scientific ...
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bioRxiv - Systems Biology 2023Quote: The DNA library was co-transfected with pCMV-dR8.91 and pMD2.G plasmids using TransIT-Lenti (Mirus) into HEK293 cells ...
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bioRxiv - Microbiology 2023Quote: ... and 1 µg PMD2.G packaging plasmids using 25 µL TransIT-LT1 Transfection Reagent (Mirus, MIR 2306). After 72 h of transfection ...
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bioRxiv - Cancer Biology 2020Quote: ... 7.5ug PsPax2 and 2.5ug VSV-G using TransIT-LT1 transfection reagent via manufacturer instructions (Mirus; Cat#: MIR 2305). FUGW (Addgene ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were transfected with 1.5 µg of the pHR plasmid along with two plasmids containing the lentiviral packaging proteins (0.167 µg of pMD2.G and 1.3 µg of p8.91) with TransIT-293 (Mirus Bio). After 2–3 d of transfection ...
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bioRxiv - Cell Biology 2020Quote: ... together with the packaging plasmids psPAX2 (UK1701) and pMD2.G (UK1700) at a 10:7.5:5 ratio using TransIT-293 reagent (Mirus). The supernatant containing the viral particles was collected 48 hrs after transfection ...
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bioRxiv - Cell Biology 2022Quote: ... and 1 μg PMD2.G packaging plasmids using 25 μl TransIT®-LT1 Transfection Reagent (Cat# MIR 2306, Mirus). After 72 h of transfection ...
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bioRxiv - Cancer Biology 2019Quote: ... in combination with 3μg psPAX2 (packaging plasmid) and 2μg pMD2.G (envelope plasmid) with 30μL TransIT-LT1 transfection reagent (Mirus, MIR 2300). For T-ALL cell infection ...
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bioRxiv - Cell Biology 2023Quote: ... and transfected with 1.5 µg of the indicated plasmid DNA and 0.5 µg of the PiggyBac transposase construct using TransIT-X2 (MIR6004, Mirus) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... the packaging plasmids psPAX2 (UK1701) and pMD2.G (UK1700) at 10:7.5:5 ratio using TransIT-293 Transfection Reagent (MIR2704, Mirus) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... pTRIPz or pLKO.1 plasmid was co-transfected with lentiviral Packaging vectors (pMD2.G and psPAX2) into HEK293T using TransIT-LT1 (Mirus) transfection reagent following the manufacturer instruction ...
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bioRxiv - Genomics 2021Quote: ... and pMD2.G at a ratio of 9:8:1 by mass using TransIT-LT1 transfection reagent (Mirus MIR 2306) at a ratio of 3 μL transfection reagent per 1 μg plasmid ...
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bioRxiv - Synthetic Biology 2021Quote: ... with a pHR’SIN:CSW transgene expression vector and the viral packaging plasmids pCMVdR8.91 and pMD2.G using Mirus Trans-IT Lenti (Mirus #MIR6606). Primary T cells were thawed the same day ...
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Antigen-dependent inducible T cell reporter system for PET imaging of breast cancer and glioblastomabioRxiv - Synthetic Biology 2022Quote: ... with a pHR’SIN:CSW transgene expression vector and the viral packaging plasmids pCMVdR8.91 and pMD2.G using Mirus Trans-IT Lenti (Mirus #MIR6606). Primary T cells were thawed the same day ...
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bioRxiv - Immunology 2022Quote: ... were transfected with pHR SIN including cloned transgene and packaging vectors pMD2.G and pCMVdR8.91 using TransIT-Lenti Transfection Reagent (Mirus #MIR6603). On the day of transfection ...
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bioRxiv - Cancer Biology 2020Quote: ... and pMD2.G (VSVG envelope vector) vectors were transfected into HEK293T cells in 94 mm2 dish using TransIT-LT1 Transfection Reagent (Mirus). The supernatants containing virus particles was collected at 72 hours after transfection ...
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bioRxiv - Neuroscience 2023Quote: ... and 20.5 µg pcDNA3.1 VSV-G in 4.9 ml DMEM with 345 µl TransIT-Lenti Transfection Reagent (Mirus Bio, MIR 6600) added to 4.8 ml DMEM ...
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bioRxiv - Cell Biology 2023Quote: HEK293T cells were transfected with retroviral packaging plasmids (pVSV-G, pBS-CMV-gagpol) and pMRX-IP-HaloTag7-LC3 using TransIT-LT1 (Mirus). The viral supernatants were collected 72 hr after transfection followed by virus concentration using Lenti-X concentrator (TaKaRa) ...