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Citations for Mirus Bio :
1 - 50 of 573 citations for Dengue Virus Serotype 3 Envelope Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2021Quote: ... torque teno virus hypothetical protein or human respirovirus 3 D protein were transfected into the cells using TransIT-LT1 (Mirus Bio) according to manufacturer’s protocol ...
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bioRxiv - Genomics 2023Quote: ... and VSV-G envelope protein using TransIT®-LT1 Transfection Reagent (Mirus) with ViralBoost Reagent (Alstem ...
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bioRxiv - Molecular Biology 2021Quote: ... Recombinant baculoviruses were recovered by transfection of the bacmids into Sf9 insect cells using TransIt-Insect reagent (Mirus). Viruses were then used to infect suspension High Five cells grown in Express5 media and supernatants harvested ∼60–72 hours post-infection ...
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bioRxiv - Cancer Biology 2023Quote: ... Sf9 cells were transfected with TransIT-insect transfection reagent (Mirus) and 5-10μg of plasmid following manufacturer’s instructions ...
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bioRxiv - Biochemistry 2019Quote: ... The plasmid was co-transfected into S2 insect cells with 10:1 molar excess of pCoBlast using TransIT-Insect transfection reagent (Mirus Bio). Stable cell lines were selected with 100 µg/mL blasticidin (Cambridge Bioscience) ...
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bioRxiv - Cell Biology 2020Quote: ... cells were incubated with plasmid DNA and TransIT-Insect Reagent (Mirus), followed by selection with 2 μg/ml puromycin (Life technologies ...
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bioRxiv - Biophysics 2023Quote: ... and envelope vector (VSV-G) into HEK293T cells using TransIT-LT1 (Mirus Bio). Supernatant post-48/72h transfection was collected for transduction in target cells supplemented with 8 µg/mL polybrene (EMD Millipore) ...
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bioRxiv - Microbiology 2019Quote: ... 100ng of RIG-I agonist derived from the 3’-UTR of hepatitis C virus (55) was transfected per 1e6 cells using TransIT-mRNA transfection kit (Mirus). For stimulation of MDA5 signaling ...
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bioRxiv - Microbiology 2019Quote: ... 100ng of RIG-I agonist derived from the 3’-UTR of hepatitis C virus (19) was transfected per 1e6 cells using TransIT-mRNA transfection kit (Mirus). For stimulation of MDA5 signaling ...
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bioRxiv - Biochemistry 2022Quote: ... Mirus Transit insect Transfection Reagent (Mirus bio) was used for the transfections according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2020Quote: ... pCMVΔR8.91 and pMD2VSV-G envelope plasmids into HEK293T cells using TransIT-LTI transfection reagent (Mirus Bio). Virus particles collected at 48 hours post-transfection were passed through a 0.45 μm filter and added to SVOG3e cells at a M.O.I ...
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bioRxiv - Cancer Biology 2019Quote: ... and envelope (VSV-G) using TransIT-LT1 (Mirus Bio). Supernatant was collected at 48 and 72 hours and applied to target cells with 8 μg/mL polybrene (EMD Millipore ...
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bioRxiv - Genomics 2021Quote: ... and packaging plasmids for expression of HIV-1 gag/pol and rev (+/-tat) and VSV-G envelope protein using either TransIT®-LT1 Transfection Reagent (Mirus) or Polyethylenimine (Polysciences ...
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bioRxiv - Molecular Biology 2023Quote: ... and rev genes and pMD2.G encoding the VSV-G envelope protein using the TransIT-Lenti transfection reagent (Mirus, MIR 6650). The virus was harvested after 48 hours and filtered through a 0.45 μm filter ...
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bioRxiv - Cell Biology 2023Quote: ... The construct was transfected into low pass S2 cells using TransIT Insect transfection reagent (Mirus) and stable cells carrying indels for this locus were selected for with 5 μg/ ml puromycin for 3 weeks ...
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bioRxiv - Biochemistry 2021Quote: ... or TransIT-Insect Transfection Reagent (Cat# MIR6100, Mirus) following modified manufacturer instruction ...
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bioRxiv - Cancer Biology 2022Quote: ... and VSV-G envelope vector mixed with TransIT-293 (Mirus). Virus-containing supernatant was collected 48 ...
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bioRxiv - Cancer Biology 2022Quote: ... and VSV-G envelope vector mixed with TransIT-293 (Mirus). Virus-containing supernatant was collected 36 ...
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bioRxiv - Genetics 2021Quote: ... 1ug of plasmid was transfected per 1ml of cells using TransIT®-Insect Transfection Reagent (Mirus, #6100) as per the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... Confirmed plasmids were transformed into S2 cells using TransIT Insect Transfection Reagent (Mirus Bio, Madison, WI, USA). Transformed cells were grown for 48 h before use in experiments.
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bioRxiv - Cell Biology 2020Quote: ... Cells were transfected with 5 μg of pACT-GFP-actin using TransIT-Insect transfection reagent (Mirus Bio) and incubated for 2 d at 28°C in Grace’s media with 10% FBS and antibiotics (100 μg/ml penicillin/streptomycin and 0.25 μg/ml Amphotericin B) ...
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bioRxiv - Molecular Biology 2022Quote: ... were co-transfected using TransIT-Insect Transfection reagent (Mirus, MIR6105). Cells were harvested 48 hrs post-transfection and samples were processed and analyzed following the procedure described above.
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bioRxiv - Cancer Biology 2020Quote: ... and pMD2.G (VSVG envelope vector) vectors were transfected into HEK293T cells in 94 mm2 dish using TransIT-LT1 Transfection Reagent (Mirus). The supernatants containing virus particles was collected at 72 hours after transfection ...
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bioRxiv - Cell Biology 2020Quote: ... adherent Sf21 cells were transfected with plasmids expressing ARIF-1 using TransIT-Insect transfection reagent (Mirus Bio, Madison, WI). At 3 d post-transfection ...
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bioRxiv - Immunology 2022Quote: ... with Trans IT-Virus GEN (Mirus). In brief ...
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bioRxiv - Cell Biology 2019Quote: ... 10cm-plate cultures of indicated cell lines were transfected/infected with indicated constructs using TransIT-Insect transfection reagent (Mirus, #MIR6100) for S2 cells ...
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bioRxiv - Molecular Biology 2023Quote: ... pAC5.1 vectors of CLIP-CRYΔ and TIM- SNAP-HA-ALFA were delivered into S2 cells using the TransIT-Insect Transfection Reagent (Cat# MIR6100, Mirus) following a modified version of the manufacturer’s instruction ...
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bioRxiv - Neuroscience 2021Quote: ... using 2 μL of TransIT-Insect transfection reagent (Mirus Bio Cat #6104). Medium was replaced with serum-containing medium 5 hours after transfection ...
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bioRxiv - Cancer Biology 2021Quote: ... d8.9 packaging vector and VSV-G envelope vector mixed with TransIT-293 (Mirus Bio). Virus containing supernatant was collected at 36 ...
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bioRxiv - Cell Biology 2023Quote: ... and 1.25 µg of the pCMV-VSVG envelope plasmid using TransIt-Lenti (MIR6604, Mirus). Three days after transfection ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Baculoviruses were produced by transfecting Sf9 cells with recombinant bacmids using TransIT®-Insect Transfection Reagent (Mirus, cat no. MIR 6100), grown for 7 days at 28ºC ...
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bioRxiv - Cell Biology 2019Quote: ... and a circular Rpl13a repair template into cultured Drosophila S2 cells using TransIT Insect Transfection Reagent (Mirus Bio LLC, Madison, WI, USA). Gene editing efficiency of the different sgRNAs was evaluated five days after transfection by cellular fluorescence and genotyping using a primer outside of the homology arm and the other inside the inserted PQR sequence.
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bioRxiv - Cell Biology 2022Quote: ... the packaging plasmid psPAX2 and the envelope plasmid pVsVg using TransIT- Lenti transfection reagent (Mirus). The transfection mix was incubated for 20 min at RT ...
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bioRxiv - Microbiology 2021Quote: ... and the VSV-G-envelope-expressing plasmid pMD2G as well as pRDI292 into 293T cells with TransIT-LT1 transfection reagent (Mirus Bio LLC, Madison, WI, USA).
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bioRxiv - Cell Biology 2022Quote: ... Drosophila constructs were transfected with plasmid DNA using TransIT®-Insect transfection reagent (Mirus Bio).
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bioRxiv - Microbiology 2019Quote: ... MHV68 was produced by first making p0 virus by transfecting NIH 3T3 cells in 6-well plates with 2.5 μg BAC DNA using TransIT-X2 (Mirus Bio) for 24h ...
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bioRxiv - Cancer Biology 2019Quote: ... in combination with 3μg psPAX2 (packaging plasmid) and 2μg pMD2.G (envelope plasmid) with 30μL TransIT-LT1 transfection reagent (Mirus, MIR 2300). For T-ALL cell infection ...
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bioRxiv - Genomics 2023Quote: ... 4ug of packing plasmids psPAX.2 and 2ug of the envelope vector pMD2.G diluted in OptiMEM medium and Trans-Ltl transfection reagent (Mirus). For the generation of gRNA lentivirus ...
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bioRxiv - Microbiology 2021Quote: ... Virus inoculum was removed after 1 h and cells were transfected with a mixture of plasmids using TransIT-LT1 (Mirus Bio # MIR 2300), including pVSV-eGFP-deltaG_SARS-CoV-2 Spike (5 µg) ...
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bioRxiv - Developmental Biology 2023Quote: ... we transfected S2 cells with pAC5.1-mcd8-CIBN-NTEV-NICD-GFP and pAC5.1-Cry2-mCherry-CTEV using the TransIT-Insect Reagent (Mirus, MIR #6104) according to manufacturer’s recommendation ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Cell-free viruses were produced by transfection of HEK293 cells with pNL4-3 using TransIT-LT1 (Mirus) or Fugene HD (Roche ...
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bioRxiv - Bioengineering 2022Quote: Transfection of human pancreatic MIA PaCa-2 and BxPC-3 cells was performed with TransIT-mRNA (Mirus Bio) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... MCF10A cells were treated with an additional 750 μL of medium containing 3 μM puromycin (Mirus Bio 5940), achieving a final concentration of 1 μM (0.5 μg/mL ...
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bioRxiv - Cancer Biology 2021Quote: ... Bait and prey plasmids containing fused fragments were then individually transfected together with VSVG and gag/pol packaging vectors in a ratio of 3:1:1 into HEK293T cells using Trans-IT Transfection reagent (Mirus) to produce retroviruses ...
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bioRxiv - Cancer Biology 2021Quote: ... and RSV-REV in the ratio of (3:1:1:1) into HEK293T cells using Trans-IT transfection reagent (Mirus). Virus-rich supernatant was collected at 72 hr post-transfection ...
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bioRxiv - Cancer Biology 2021Quote: ... Lentiviral vectors were co-transfected with packaging vectors VSVG and gag/pol in a ratio of 3:1:2 into HEK293T cells using Trans-IT Transfection reagent (Mirus). Viral supernatants were collected at 48 and 72 hr post-transfection ...
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bioRxiv - Cell Biology 2022Quote: Viral particles were produced by transfection of 293FT cells in 6-well plates with 3 μg of total DNA and 8 mL TransIT-293 Transfection Reagent (Mirus Bio) per well ...
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bioRxiv - Biophysics 2020Quote: 1μg of recombinant Plexin-B1 FL WT pCDNA 3.1 plasmid or Plexin-B1 FL mutants pCDNA 3.1 (as described above) was transfected to 80% confluent COS 7 cells on coverslips using 3 μL Trans IT 2020 transfection reagent (Mirus Bio) for 48 hours at 37 °C ...
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bioRxiv - Microbiology 2020Quote: Viral particles were produced by transfection of 293FT cells in 6-well plates with 3 μg DNA and 8 μl TransIT-293 Transfection Reagent (Mirus Bio) per well ...
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bioRxiv - Microbiology 2019Quote: ... HEK293 cells (150 cm2 dish) were transfected with 6 μg of each protein-coding plasmid by using TransIT (Mirus). Cell lysis and elution were performed as described above ...