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Citations for Mirus Bio :
1 - 50 of 184 citations for Dengue Virus Serotype 1 NS1 Protein HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2021Quote: ... torque teno virus hypothetical protein or human respirovirus 3 D protein were transfected into the cells using TransIT-LT1 (Mirus Bio) according to manufacturer’s protocol ...
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bioRxiv - Microbiology 2019Quote: ... HEK293 cells (150 cm2 dish) were transfected with 6 μg of each protein-coding plasmid by using TransIT (Mirus). Cell lysis and elution were performed as described above ...
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bioRxiv - Microbiology 2019Quote: ... HEK 293 cells were transfected with YF-NS1-GFP using TransIT®-LT1 transfection reagent (Mirus Bio LLC, Belgium), according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... HEK293 cells were transfected with TransIT-293 (Mirus) according to manufacturer’s instructions with three plasmids ...
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bioRxiv - Immunology 2022Quote: ... with Trans IT-Virus GEN (Mirus). In brief ...
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bioRxiv - Microbiology 2022Quote: ... The CPER products were then directly transfected into HEK293-C34 cells using Trans IT LT-1 (Mirus). At 6 hours post-transfection ...
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bioRxiv - Physiology 2021Quote: HEK293 cells were transiently transfected with TransIT-LT1 reagent (Mirus) with channel cDNA and hERG1a N-terminal domain cDNAs in a 2:1 ratio in co-expression experiments ...
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bioRxiv - Microbiology 2019Quote: ... 1 µg pSINrep21/YFV NS1 plasmid DNA was transfected into BHK-21 clone 15 cells by using 8 µl TransIT LT1 reagent (Mirus Bio, Wisconsin, MD) and low-serum OptiMem (Life Technologies) ...
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bioRxiv - Microbiology 2020Quote: The CPER products (25 μl out of a 50 μl reaction volume) were transfected into HEK293-3P6C33 cells or BHK-21 cells with Trans IT LT-1 (Mirus), following the manufacturer’s protocols ...
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bioRxiv - Synthetic Biology 2020Quote: ... The plated HEK293 cells were transfected using TransIT DNA transfection reagent (Mirus) following the instructions supplied by the manufacturer and incubated until use ...
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bioRxiv - Immunology 2023Quote: HEK293 cells were transfected using TransIT-293 transfection reagent (Mirus Bio LLC). For transient expression of human Siglec-5-GFP 80% confluent HEK293 in 8 well chamber slide (0.25 ml/well ...
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bioRxiv - Cell Biology 2023Quote: ... and transfected into HEK293 cells (ECACC) using TransIT-293 (Mirus Bio LLC), according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... transiently expressed in HEK293 cells using TransIT-293 transfection reagent (Mirus, Madison, WI), and characterized using whole-cell patch-clamp electrophysiology and single cell calcium microfluorimetry as described above.
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bioRxiv - Neuroscience 2023Quote: HEK293 cells were transfected to produce lentiviral particles with TransIT-Lenti transfection reagent (Mirus) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: U20S or HEK293 cells were transfected with the TransIT-XL reagent (Mirus Bio, Madison, WI) with PRK-TK-Neo plasmids encoding for ovalbumin-derived peptides with a signal sequence (MGGTAARLGAVILFVVIVGLHGVRG - based on the signal sequence of Human Herpes Virus 1 Glycoprotein D ...
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bioRxiv - Immunology 2020Quote: HeLa cells were transfected using TransIT-HeLaMONSTER and HEK293 cells using TransIT-293 (both from Mirus). Cells after transfection were cultured both with and without ProS or HS in the medium ...
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bioRxiv - Microbiology 2021Quote: ... Virus inoculum was removed after 1 h and cells were transfected with a mixture of plasmids using TransIT-LT1 (Mirus Bio # MIR 2300), including pVSV-eGFP-deltaG_SARS-CoV-2 Spike (5 µg) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Cell-free viruses were produced by transfection of HEK293 cells with pNL4-3 using TransIT-LT1 (Mirus) or Fugene HD (Roche ...
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bioRxiv - Microbiology 2021Quote: ... The half of CPER product was transfected into IFNAR1-deficient HEK293 cells TransIT-LT1 transfection reagents (Mirus), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: HEK293 were transfected with pMTBS and pCS2-ST at ~80% confluence using TransIT-293 transfection reagent (Mirus). Cells were harvested for downstream applications 48 hours post-transfection.
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bioRxiv - Cell Biology 2022Quote: ... for transfection of HEK293 Flp-In T-REx cells and 2 ul Trans-IT-HeLa and 1.3 ul Monster (MIR 2900, Mirus) for HeLa Flp-In T-REx cells ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Viral stocks of NL43 were produced by transfection of HEK293 cells with the molecular clone plasmid using TransIT-LT1 (Mirus) transfection reagent ...
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bioRxiv - Microbiology 2019Quote: ... NL4-3 and NL4-3(AD8) HIV viral stocks were produced by transfection of HEK293 cells with the molecular clone plasmids using TransIT-LT1 (Mirus) transfection reagent ...
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bioRxiv - Microbiology 2023Quote: ... Lentiviruses for transduction of LTR-mCherry reporter and expression of HIV receptors (CD4, CXCR4, and CCR5) was generated by transfection of HEK293 cells using the Mirus TransIT transfection kit (Mirus). The resulting supernatant was cleared of debris by low-speed centrifugation (300g for 5 minutes ...
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bioRxiv - Cell Biology 2022Quote: ... carrying the sequence of the gene to be over expressed and resistance to puromycin into 0.8×106 HEK293 cells in a 60 mm culture dish with 6 µl of TrasnIT (Cat. Nº: Mirus Bio. 293) containing 3 ml of complete media ...
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bioRxiv - Microbiology 2022Quote: ... in which human ACE2 and TMPRSS2 are induced by tetracycline (HEK293-3P6C33 cells) with TransIT-LT1 Transfection Reagent (Mirus, Madison, WI, USA), following the manufacturer’s protocols ...
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bioRxiv - Microbiology 2019Quote: ... MHV68 was produced by first making p0 virus by transfecting NIH 3T3 cells in 6-well plates with 2.5 μg BAC DNA using TransIT-X2 (Mirus Bio) for 24h ...
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bioRxiv - Microbiology 2019Quote: ... 100ng of RIG-I agonist derived from the 3’-UTR of hepatitis C virus (55) was transfected per 1e6 cells using TransIT-mRNA transfection kit (Mirus). For stimulation of MDA5 signaling ...
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bioRxiv - Microbiology 2019Quote: ... 100ng of RIG-I agonist derived from the 3’-UTR of hepatitis C virus (19) was transfected per 1e6 cells using TransIT-mRNA transfection kit (Mirus). For stimulation of MDA5 signaling ...
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bioRxiv - Neuroscience 2023Quote: ... H1 plasmid library with the top 5 sgRNA per gene21 were transfected into HEK293s using the TransIT®-Lenti Transfection Reagent (Mirus, Cat. No. MIR 6606) along with third generation lentiviral packaging mix ...
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bioRxiv - Genomics 2021Quote: ... and packaging plasmids for expression of HIV-1 gag/pol and rev (+/-tat) and VSV-G envelope protein using either TransIT®-LT1 Transfection Reagent (Mirus) or Polyethylenimine (Polysciences ...
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bioRxiv - Genomics 2023Quote: ... and VSV-G envelope protein using TransIT®-LT1 Transfection Reagent (Mirus) with ViralBoost Reagent (Alstem ...
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bioRxiv - Molecular Biology 2022Quote: ... RNAs encoding green fluorescent protein (GFP) were complexed with TransIT (Mirus Bio, Madison, WI) according to the manufacturer’s instructions by diluting in EMEM without serum or antibiotics and incubating for 2-5 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... cells were co-transfected with receptor of interest and TRUPATH plasmids at 1:1:1:1 DNA ratio (receptor:Gα-RLuc8:Gβ:Gγ-GFP2) via TransIT-2020 (Mirus Cat# MIR5400). Each condition required 97 µL of room-temperature 1x Opti-MEM (Gibco Cat# 31985070) ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were transfected with 1.5µg of the pHR plasmid along with two plasmids containing the lentiviral packaging proteins (0.167µg of pMD2.G and 1.3µg of p8.91) with TransIT-293 (Mirus Bio). After 2-3 days of transfection ...
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bioRxiv - Microbiology 2021Quote: ... Transient transfections for protein expression in 293T were performed with Transit-LT1 transfection reagent (Mirus Biotech) according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2023Quote: ... individual wells were transfected with plasmids encoding Venus fused BH3-proteins using TransIT-X2 reagent (Mirus). Non-transfected wells were treated with transfection reagent alone (no DNA added) ...
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bioRxiv - Microbiology 2021Quote: ... and RdRp-N462 or RdRp-D462 were transfected into 293T cells at a 1:1:1 ratio using TransIT LT-1 (Mirus, Madison, WI). To normalize transfection efficiency ...
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bioRxiv - Developmental Biology 2021Quote: ... protein expression (15 ng/well) and target (4.5 ng/well) plasmids using TransIT-2020 Transfection Reagent (Mirus) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... GFP- and FLAG-tagged proteins under the control of the Actin promoter using TransIT-LT1 reagent (Mirus). 24-40 h after transfection ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were transfected with 1.5 µg of the pHR plasmid along with two plasmids containing the lentiviral packaging proteins (0.167 µg of pMD2.G and 1.3 µg of p8.91) with TransIT-293 (Mirus Bio). After 2–3 d of transfection ...
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bioRxiv - Cancer Biology 2019Quote: ... Lentivirus expressing RIPK3 shRNA was generated by transfecting HEK-293T cells in 6 well plate with a 1: 0.1: 1 ratio of pMDG2: pVSVG: pLKO.1 with TransIT-LT1 transfection reagent (Mirus). After filtering through 0.45 μm of cellulose acetate membrane (VWR ...
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bioRxiv - Biochemistry 2024Quote: ... and TRUPATH plasmids at 1:1:1 DNA ratio (Gα-RLuc8:Gβ:Gγ-GFP2) via TransIT-2020 (Mirus Cat# MIR5400). Each condition required 97 µL of room-temperature 1x Opti-MEM (Gibco Cat# 31985070) ...
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bioRxiv - Biophysics 2021Quote: ... δ and ε subunits in the ratio 2:1:1:1 (TransIT® 293 transfection reagent; Mirus Bio, Madison, WI). Electrophysiological experiments started ~48 hours post-transfection ...
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bioRxiv - Cancer Biology 2021Quote: ... and RSV-REV in the ratio of (3:1:1:1) into HEK293T cells using Trans-IT transfection reagent (Mirus). Virus-rich supernatant was collected at 72 hr post-transfection ...
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bioRxiv - Microbiology 2019Quote: ... HEK293 cells in 6 well plates were transfected with 500 ng/well of each protein-coding plasmid by using TransIT (Mirus). After 48 h ...
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Potassium channel-driven bioelectric signaling regulates metastasis in triple-negative breast cancerbioRxiv - Cancer Biology 2021Quote: ... Constructs were mixed with 0.5μg helper (encoding gag/pol) and 0.1μg encoding coat protein (pMD2) along with 6μL TransIT-Lenti transfection reagent (MIR6603; Mirus Bio; Madison, WI) in 200uL Opti-MEM (31985062 ...
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bioRxiv - Microbiology 2020Quote: ... In vitro transcribed full-length CHIKV mRNA and mRNA encoding single viral proteins was transfected into target cells with the TransIT mRNA kit (Mirus). MAYV (strain TRVL15537 ...
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bioRxiv - Immunology 2020Quote: HEK293T cells were transiently transfected with mRNA encoding SARS-CoV-2 WT S or S-2P protein using a TranIT mRNA transfection kit (Mirus). After 24 hr ...
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bioRxiv - Systems Biology 2021Quote: ... and grown in complete media for 1 day before co-transfection of the mammalian expression vectors encoding GFP and viral protein of interest using TransIT-2020 transfection reagent (#MIR5404, Mirus). After 24 h ...