Labshake search
Citations for Bachem :
1 - 50 of 50 citations for Transglutaminase Substrate biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... Bz-Phe-Val-Arg-AMC.HCl (Thrombin substrate III) and Boc-Gln-Ala-Arg-AMC.HCl (Trypsin substrate) were from Bachem, Suc-Ala-Ala-Pro-Phe-AMC (Chymotrypsin substrate II ...
-
bioRxiv - Immunology 2022Quote: ... The substrate DABCYL-KTSAVLQ↓SGFRKM-EDANS (Bachem) was added ...
-
bioRxiv - Biochemistry 2020Quote: Synthetic human Aβ residues 1-42 and biotinylated Aβ42 were purchased from Bachem or AnaSpec and solubilized in hexafluoroisopropanol (HFIP ...
-
bioRxiv - Biochemistry 2019Quote: ... was from R&D Systems and legumain substrate (Z-AAN-AMC) was from Bachem. The cathepsins assay buffer composition was ...
-
bioRxiv - Systems Biology 2019Quote: ... The fluorogenic proteasome substrate Suc-LLVY-AMC (Bachem I-1395) was added to a final concentration of 100 μM ...
-
bioRxiv - Pathology 2023Quote: ... Substrate was bought from Bachem (I-1875; Boc-Lys (Ac)-AMC) ...
-
bioRxiv - Cancer Biology 2020Quote: ... APEH activity was measured by chromogenic substrate acetyl-Ala-pNa (Bachem) [37] ...
-
bioRxiv - Cell Biology 2023Quote: ... HCl (417 μM substrate, 6.25 mM CaCl2 final concentration, Bachem Holding) in TGA dilution buffer (20 mM HEPES ...
-
bioRxiv - Molecular Biology 2023Quote: ... proteasomal activity was assessed using the Suc-LLVY-AMC substrate (Bachem). 50µg of total protein extract were diluted with lysis buffer in a final volume of 190µl in 96-well black microtiter plates ...
-
bioRxiv - Systems Biology 2022Quote: ... a fluorogenic substrate was used (Suc-Leu-Leu-Val-Tyr-AMC, Bachem ## I-1395 ...
-
bioRxiv - Microbiology 2021Quote: ... which measures protease activity with the short peptide substrate Z-RLRGG-AMC (Bachem), was performed in black ...
-
bioRxiv - Pathology 2021Quote: ... ACE2 enzyme activity was measured using Mca-APK-Dnp substrate (Bachem, Bubendorf, Switzerland).
-
bioRxiv - Biochemistry 2021Quote: Peptidase assays with fluorogenic Boc-Gln-Ala-Arg-AMC substrate (Bachem Cat # 4017019.0025) were performed in 96-well plates (Greiner Fluotrak ...
-
bioRxiv - Biochemistry 2021Quote: ... The fluorogenic Z-FR-AMC dipeptide substrate (Bachem, cat # I-1160, HCl salt) was prepared as a 32.1 mM stock solution in DMSO.
-
bioRxiv - Biochemistry 2022Quote: Hydrolysis of the fluorogenic-peptide substrates suc-LLVY-AMC (7-amino-4-methylcoumarin; Bachem) was quantified to determine the proteolytic activity of proteasomes ...
-
bioRxiv - Microbiology 2021Quote: ... There was no activity as expected with the cysteine protease substrate sLY-AMC (Bachem; Product #4002047 ...
-
bioRxiv - Physiology 2020Quote: ... Chymotrypsin (Suc-AAPF-AMC) and Cathepsin B substrates (AMC-Arg2) were obtained from Bachem AG (Bubendorf ...
-
bioRxiv - Pathology 2021Quote: ... total ACE2 enzyme activity was measured using Mca-APK-Dnp substrate (Bachem, Bubendorf, Switzerland). For staining studies ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... The fluorogenic substrates Boc-QAR-AMC and Boc-VPR-AMC were purchased from Bachem AG (Bubendorf ...
-
bioRxiv - Molecular Biology 2023Quote: ... 1 mM DTT) with 0.05 mM substrate Suc-Leu-Leu-Val-Tyr-AMC (Bachem). ChemiDoc MP (Bio-Rad ...
-
bioRxiv - Neuroscience 2024Quote: ... 1 mM DTT and 200 µM substrate (Z-Ala-Ala-Asn-AMC (# l-1865.0050, BACHEM)) ...
-
bioRxiv - Biophysics 2021Quote: ... The FRET substrate DABCYL-KTSAVLQ↓SGFRKM-E(EDANS) trifluoroacetate salt was purchased from Bachem (PN 4045664), dissolved to 10 mM in DMSO and stored in aliquots at −20 °C ...
-
bioRxiv - Microbiology 2021Quote: ... reaction was started without further incubation by adding the substrate Boc-Leu-Arg-Arg-AMC (Bachem. Bubendorf BL ...
-
bioRxiv - Pathology 2022Quote: ... 1 mM DTT) with 0.05 mM chymotrypsin-like substrate Suc-Leu-Leu-Val-Tyr-AMC (Bachem) for 30 min at 37 °C ...
-
bioRxiv - Biochemistry 2022Quote: ... Proteolytic activity was measured using Z-Arg-Leu-Arg-Gly-Gly-AMC substrate (Bachem, 369 I1690) as described previously in Ashhurst et al ...
-
bioRxiv - Biochemistry 2022Quote: ... Thrombin substrate benzyloxycarbonyl-glycyl-glycyl-arginine 7-amido-4-methylcoumarin (ZGGR-AMC) was purchased from Bachem AG ...
-
bioRxiv - Biochemistry 2021Quote: ... 1 mM DTT) with 0.05 mM chymotrypsin-like substrate Suc-Leu-Leu-Val-Tyr-AMC (Bachem) for 30 min at 37 °C ...
-
bioRxiv - Biochemistry 2022Quote: ... In-gel peptidase assays were conducted using the fluorogenic peptide substrate LLVY-AMC (Bachem, I-1395.0100) as described previously (32) ...
-
bioRxiv - Bioengineering 2023Quote: ... or an MMP-degradable FXIII lysine donor substrate (Lys-MMPsensitive peptides; Ac-FKGGGPQGIWGQ-ERCG-NH2; Bachem), resulting in 8-PEG-Gln or 8-PEG-MMPsensitive-Lys precursors ...
-
bioRxiv - Physiology 2023Quote: ... 1 mM MgCl2) with 0.05 mM chymotrypsin-like substrate Suc-Leu-Leu-Val-Tyr-AMC (Bachem) at 37 °C for 30 min ...
-
bioRxiv - Neuroscience 2019Quote: ... N-succinyl-Leu-Leu-Val-Tyr-7-amino-4-methylcoumarin (Suc-LLVY-AMC) substrate was used (BACHEM). GST-Ubl and His-UIM constructs were a gift from the Alfred Goldberg lab ...
-
bioRxiv - Biochemistry 2023Quote: The peptidase activity of the 20S proteasome was measured with commonly used fluorogenic peptide substrates (Bachem, Calbiochem): succinyl-LeuLeuValTyr-4-methylcoumarin-7-amide (SucLLVY-MCA ...
-
bioRxiv - Cell Biology 2022Quote: ... TA substrates were generated that contained the unnatural amino acid and photocrosslinker 4-Benzoylphenylalanine (BpA) (Bachem, Switzerland), which was incorporated into the TMD by amber stop codon suppression in the PURExpress system lacking all release factors (ΔRF123 ...
-
bioRxiv - Biochemistry 2021Quote: SARS-CoV-2 3CLpro activity assay was performed using a fluorogenic substrate DABCYL- KTSAVLQ↓SGFRKME-EDANS (Bachem, Switzerland) in a buffer containing 20 mM Tris pH 7.2 ...
-
bioRxiv - Genetics 2019Quote: ... Chymotrypsin-like activity of the separated proteasome complexes was then measured by incubating 0.1mM of the suc-LLVY-AMC substrate (Bachem) at 37°C for 20 min in a final volume of 10 ml of overlay buffer (20 mM Tris ...
-
bioRxiv - Physiology 2020Quote: ... The chymotrypsin (β5)-like activities were assayed using 10μg of protein and the fluorescently tagged substrate SUC-LLVY-AMC (Bachem). Both assays were carried out in a total volume of 100μl ...
-
bioRxiv - Immunology 2023Quote: ... 20µL Boc-Glu-Lys-Lys fluorometric substrate was added to each well to enable plasmin detection (final concentration, 1260µM; Bachem). Substrate was diluted in 34mM CaCl2 containing TBS ...
-
bioRxiv - Immunology 2024Quote: ... The other solution contained a 7.5 mM di-cysteine modified Matrix Metalloprotease (MMP) peptide substrate (Ac-GCRDGPQGIWGQDRCG-NH2) (Bachem). RGD ...
-
bioRxiv - Immunology 2021Quote: ... and incubated with the thrombin substrate Z-Gly-Gly-Arg 7-amido-4-methylcoumarin hydrochloride (0.42 mM) (Bachem AG, Switzerland) and CaCl2 (15 mM ...
-
bioRxiv - Biochemistry 2021Quote: ... a fluorogenic MPro substrate DABCYL-Lys-Thr-Ser-Ala-Val-Leu-Gln-Ser-Gly-Phe-Arg-Lys-Met-Glu-EDANS from Bachem, and K777 as a gift from Prof ...
-
bioRxiv - Microbiology 2020Quote: The proteolytic activity of NS2B-NS3pro was measured using a fluorescence resonance energy transfer-based assay with a fluorogenic peptide substrate (Boc-Gly-Arg-Arg-AMC, No: I-1565, Bachem) as the substrate ...
-
bioRxiv - Biochemistry 2022Quote: ... Residual uPA activity was assayed by the rate of proteolysis of the fluorescent substrate Z-Gly-Gly-Arg-AMC (Bachem) over a 10 min time course (λem = 370 nM ...
-
bioRxiv - Bioengineering 2023Quote: ... NOF Europe) was functionalized with peptides that contained either an FXIII glutamine acceptor substrate (Gln peptides; NQEQVSPL-ERCG-NH2; Bachem) or an MMP-degradable FXIII lysine donor substrate (Lys-MMPsensitive peptides ...
-
bioRxiv - Microbiology 2019Quote: ... using as putative substrates the free amino acid glutamine and the C-terminally amidated peptide epinecidin-1 (sequence: GFIFHIIKGLFHAGKMIHGLV-NH2) (Bachem AG). Glutamine (10 mM ...
-
bioRxiv - Biochemistry 2019Quote: ... Thrombin generation was initiated by adding 20 µl Hepes/BSA buffer containing 100 mM CaCl2 and 2.5 mM fluorogenic substrate Z-Gly-Gly-Arg-AMC (Bachem, Bubendorf, Switzerland). Tissue factor-dependent thrombin generation was assessed with PPP Reagent low (1 pM tissue factor in the presence of 4 µM phospholipids ...
-
bioRxiv - Biochemistry 2021Quote: ... Plasma was incubated with the thrombin substrate Z-Gly-Gly-Arg 7-amido-4-methylcoumarin hydrochloride (0.42 mM) (Bachem AG, Switzerland) and CaCl2 (15 mM ...
-
bioRxiv - Biochemistry 2020Quote: SARS-CoV-2 3CLpro activity assay was performed as described earlier using a fluorogenic substrate DABCYL-KTSAVLQ↓SGFRKME-EDANS (Bachem, Switzerland) in a buffer containing 20 mM Tris pH 7.2 ...
-
bioRxiv - Molecular Biology 2021Quote: The proteolytic activities of PR were determined using the principles of Förster resonance energy transfer (FRET) by cleavage of a fluorogenic peptide substrate DABCYL-γ-abu-Ser-Gln-Asn-Tyr-Pro-Ile-Val-Gln-EDANS (Bachem, Germany) with DABCYL ...
-
bioRxiv - Microbiology 2021Quote: Protease activity of recombinant Mpro was measured using the quenched fluorogenic substrate {DABCYL}-Lys-Thr-Ser-Ala-Val-Leu-Gln-Ser-Gly-Phe-Arg-Lys-Met-Glu- (EDANS)-NH2 (Bachem, Vista, CA, USA). 5 μL of 25 nM Mpro diluted in assay buffer [25 mM HEPES (pH 7.4) ...
-
bioRxiv - Cell Biology 2021Quote: ... 4 ug protein were incubated with 50 uM Suc–Leu–Leu–Val–Tyr– AMC peptide substrate (chymotrypsin-like activity; Bachem, cat. no. I-1395) or 50 uM Ac– NIe–Pro–NIe–Asp–AMC (caspase-like activity ...