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Citations for Biosearch Technologies :
1 - 50 of 57 citations for hsa let 7f RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... a set of oligonucleotides spanning the first intron of let-7-C was designed using the Stellaris Probe Designer software (Biosearch Technologies). The oligos were ordered from Biosearch Technologies labeled with Quasar-670.
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bioRxiv - Molecular Biology 2021Quote: ... Two allele specific forward primers and a reverse primer were designed for use in fluorescence based competitive allele-specific PCR assays (KASP; LGC Biosearch Technologies). DNA from the 286 lines was assayed using these primers and KASP reaction mix (LGC Biosearch Technologies ...
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bioRxiv - Biochemistry 2023Quote: ... The ∼105 nt cDNAs were separated from free RT primers on a 15% TBE-Urea gel and circularized using CircLigaseII ssDNA Ligase (Biosearch Technologies; CL9021K). PCR was carried out using forward primer NI-798 ...
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bioRxiv - Developmental Biology 2021Quote: ... Probes sets for smFISH (Biosearch Technologies) or smiFISH (Sigma Aldrich ...
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bioRxiv - Immunology 2021Quote: ... SARS-CoV-2 genome equivalents were quantified in culture supernatant by RT-qPCR using 2019-nCoV CDC probe and Primer Kit for SARS-CoV-2 (Biosearch Technologies, KIT-nCoV-PP1-1000). Forward primer ...
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bioRxiv - Cancer Biology 2023Quote: ... The EZH2 probe set was ordered from the Stellaris Design Ready Probe Sets (Biosearch Technologies VSMF-2123-5). All other RNA-FISH probe sets were custom designed using the Stellaris Probe Designer tool at the biosearchtech.com website ...
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bioRxiv - Systems Biology 2019Quote: Stellaris probe-sets for smFISH (Biosearch Technologies) were custom designed for intron and exon regions (see Appendix for oligo list ...
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bioRxiv - Developmental Biology 2020Quote: Exonic probe sets for smFISH (Biosearch Technologies) were conjugated to Quasar 570 fluorophores (Table S1).
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bioRxiv - Systems Biology 2019Quote: ... Custom probe sets for sm FISH (Biosearch Technologies) were hybridized at a final concentration of 0.1 μM probe following manufacturers instructions over night at 37°C ...
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bioRxiv - Microbiology 2021Quote: ... The abundance of SARS-CoV-2 genetic material was subsequently determined by quantitative PCR using a Taqman probe and primers (Biosearch Technologies). The primers and probe sequences for the were as recommended by BEI resources for compatability with synthetic RNA standards (# NR-52358).
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bioRxiv - Plant Biology 2020Quote: ... allele-specific forward primers and one common reverse primer (KASP; LGC Biosearch Technologies, Beverley, MA). Each forward primer incorporates an additional tail sequence that corresponds with one of two universal FRET (fluorescent resonance energy transfer ...
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bioRxiv - Immunology 2019Quote: ... Hybridization was carried out using DNA probe sets (Biosearch Technologies) according to the protocol provided by Biosearch Technologies ...
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bioRxiv - Molecular Biology 2019Quote: ... and incubated overnight with custom Stellaris oligonucleotide sets (Biosearch Technologies) labelled with CAL Fluor Red 610 ...
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Size-Dependent Expression of the Fission Yeast Cdc13 Cyclin is Conferred by Translational RegulationbioRxiv - Cell Biology 2023Quote: ... and incubated over-night with custom Stellaris oligonucleotides sets (Biosearch Technologies) designed against cdc25 (CAL Fluor Red 610 ...
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bioRxiv - Biophysics 2020Quote: ... We ordered the probes as a Stellaris Custom Probe set (Biosearch Technologies) and they are listed in Table S4 ...
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bioRxiv - Microbiology 2022Quote: ... Stellaris RNA FISH probe sets were used (Biosearch Technologies, Novato, CA, USA) (Table S2 ...
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bioRxiv - Cancer Biology 2021Quote: ... For hybridization 125 nM of the probe set in hybridization buffer (Biosearch Technologies) was added and incubated overnight at 37 0C ...
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bioRxiv - Genomics 2020Quote: We used a custom-synthesized 48-probe set (LGC Biosearch Technologies; Petaluma, CA) of non-overlapping fluorescent-tagged oligonucleotides that tiled the 3.4 kb LOC157273 transcript ...
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bioRxiv - Developmental Biology 2022Quote: Probe sets for smFISH were generated using the online Stellaris (LGC Biosearch Technologies) probe designer ...
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bioRxiv - Neuroscience 2022Quote: Probe sets targeting the 3’UTR of rat Atf3 (Stellaris probes, Biosearch technologies) were designed using the Stellaris probe-set designer tool to specifically detect the 3’UTR of the transcript and 3’end labelled with CalFluor590 ...
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bioRxiv - Molecular Biology 2023Quote: Two sets of 3’-end biotinylated RNA oligonucleotides (LGC Biosearch Technologies; Risskov, Denmark) (Table S3 ...
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bioRxiv - Cell Biology 2024Quote: RNA FISH samples were prepared using an R2 Stellaris probe set (Biosearch Technologies) as previously described (Lu et al ...
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bioRxiv - Developmental Biology 2021Quote: ... genotyping was performed using KASP primer pairs (Biosearch Technologies) targeting the following sequence ...
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bioRxiv - Cell Biology 2020Quote: ... Both probe sets were labeled with CAL Fluor Red 590 Dye (Biosearch Technologies, Inc.). Samples were hybridized with the gag/gag-pol probe set according to the manufacturer’s instructions available online ...
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bioRxiv - Microbiology 2023Quote: ... Plasmodium 18S rRNA primers/probes (LCG BioSearch Technologies, Novato, CA) were as follows ...
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bioRxiv - Cell Biology 2022Quote: ... Plasmodium 18S rRNA primers/probes (LCG BioSearch Technologies, Novato, CA) were as follows ...
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bioRxiv - Cell Biology 2021Quote: ... a set of 46 probes coupled to the Quasar570 dye (Biosearch Technologies, Stellaris Custom Probes) were used ...
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bioRxiv - Molecular Biology 2022Quote: ... a single custom probe set was designed using Stellaris® Probe designer software (LGC, Biosearch Technologies), with cDNA sequences of the replicative histone transcripts used as the input (See probe sequences in supplementary table 1) ...
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bioRxiv - Molecular Biology 2022Quote: ... Proprietary primers were used for genotyping as previously described (LGC Biosearch technologies)25 ...
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bioRxiv - Genomics 2023Quote: ... Slides were hybridized with GREB1 or NRIP1 Stellaris FISH Probe sets labeled with Quasar 670 (Biosearch Technologies) following the manufacturer’s instructions (www.biosearchtech.com/stellarisprotocols) ...
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bioRxiv - Developmental Biology 2021Quote: ... Probe set against nos exons was designed using the Stellaris® RNA FISH Probe Designer (Biosearch Technologies, Inc.) available online at https://www.biosearchtech.com/stellarisdesigner ...
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bioRxiv - Cell Biology 2023Quote: ... eGFP transcripts were detected using the pre-designed Quasar 570 probe set (Biosearch Technologies Inc., VSMF-1014-5).
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bioRxiv - Developmental Biology 2022Quote: ... Embryos were hybridized with Stellaris RNA FISH Probe sets labeled with CalFluor 610 or Quasar 670 (Biosearch Technologies, Inc.), following the manufacturer’s instructions available online at www.biosearchtech.com/stellarisprotocols ...
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bioRxiv - Neuroscience 2021Quote: ... The cultured primary neurons were hybridized with the ADRAM Stellaris FISH Probe set labeled with TAMRA (Biosearch Technologies, Inc.), following the manufacturer’s instructions.
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bioRxiv - Systems Biology 2023Quote: ... permeabilised in ethanol and hybridized with a recB specific set of TAMRA-labelled RNA FISH probes (LGC Biosearch Technologies). Probe sequences are listed in Table 6 ...
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bioRxiv - Genomics 2022Quote: ... the pooled PCR products were purified using sbeadex PCR clean-up kits (LGC, Biosearch Technologies) by following the manufacturer’s instructions to purify the PCR band at 286 bp ...
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bioRxiv - Molecular Biology 2019Quote: ... A set of 48 Quasar 570 labeled probes were designed to target the coding sequence of Gal3 and purchased from Biosearch Technologies. Probe sequences are listed in Table S3.
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bioRxiv - Molecular Biology 2019Quote: ... was incubated for 5 minutes and ACTB probe set was hybridized in for 16 hours at 37°C with Hybridization buffer (LGC Biosearch Technologies). After hybridization ...
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bioRxiv - Neuroscience 2021Quote: ... Primary motor neurons were hybridized with the indicated Stellaris FISH Probe set labeled with either Quasar-570 or 670 (Biosearch Technologies) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... A set of Stellaris RNA FISH Probes labeled with Cy5 dye specific to Hmrhl targeted transcript was purchased from Biosearch Technologies. Briefly ...
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bioRxiv - Developmental Biology 2020Quote: ... The embryos were hybridized with Stellaris RNA FISH Probe sets labeled with Cal Fluor 610 or Quasar 670 (Biosearch Technologies, Inc.), following the manufacturer’s instructions available online at www.biosearchtech.com/stellarisprotocols ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were then stained for 16 h with 125 nM of a custom KILR Stellaris RNA-FISH probe set labelled with Quasar 570 fluorophore (LGC Biosearch Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... Custom DNA probe sets targeting different regions of RNAs of interest were designed using Stellaris® Probe Designer and either synthesized by Biosearch Technologies containing 3’ amine-reactive group or ordered as DNA oligos from Biobasic and ThermoFisher and modified in house by the addition of amine-modified ddUTP as previously described in Gaspar et al ...
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bioRxiv - Systems Biology 2020Quote: ... The probe sets targeting Il6 and Csf2 were designed using the Stellaris® RNA FISH Probe Designer (Biosearch Technologies, Inc., Petaluma, CA) available online (www.biosearchtech.com) ...
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bioRxiv - Systems Biology 2023Quote: ... each cover glass was set on a droplet (cells facing down) consisting of 45 μL hybridization buffer (Biosearch Technologies, SMF-HB1-10) and 1 μL of the duplex smiFISH probes (MS2-transcript-binding probe mix + FLAP-Y-binding region annealed to FLAP-Y-Cy5 ...
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bioRxiv - Molecular Biology 2021Quote: ... DNA from the 286 lines was assayed using these primers and KASP reaction mix (LGC Biosearch Technologies) according to manufacturer’s instructions ...
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bioRxiv - Genetics 2021Quote: ... The mock-treated or infected nematodes were hybridized with the Stellaris RNA FISH Probe set labeled with CAL Fluor® Red 590 Dye (Biosearch Technologies, Inc.), following the manufacturer’s instructions available online at www.biosearchtech.com/stellarisprotocols based on protocols by Raj et al ...
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bioRxiv - Genetics 2022Quote: ... and stored at 4°C for several days. A lag-2 RNA probe set (Barkoulas et al. 2013) coupled to AF594 (Custom Stellaris Fish Probes, Biosearch Tech, Teddington UK) was resuspended in RNase-free TE buffer (pH8 ...
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bioRxiv - Microbiology 2024Quote: ... FailSafe PCR 2X PreMix D or G (LCG, Biosearch Technologies) were used as buffer for all PCR reactions.
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bioRxiv - Plant Biology 2024Quote: ... homoeolog-specific KASP (Kompetitive Allele-Specific PCR, LGC Biosearch Technologies) primers were designed using Polymarker (Ramirez-Gonzalez ...