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Citations for Anaspec :
1 - 32 of 32 citations for Transglutaminase Substrate biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... was incubated with the substrate of H3(1-21) lysine 9 tri-methylated biotinylated peptide (30 nM, Anaspec), in the presence of aKG (10 μM ...
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bioRxiv - Neuroscience 2021Quote: ... small peptide biomarker which is a substrate for MMP2/9 (A580, MMP Substrate 1, AnaSpec).
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bioRxiv - Biochemistry 2021Quote: ... the substrate FAM-QRVRRAVGIDK-TAMRA (AnaSpec # 808143 ...
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bioRxiv - Microbiology 2021Quote: ... and exposed for 3h to 500nM or 5μM biotinylated CGRP (AnaSpec) in 100μl RPMI medium added to the apical side ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Peptide substrates were from AnaSpec (Fremont, CA). α-Ketoglutarate (disodium salt dihydrate ...
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bioRxiv - Biophysics 2023Quote: ... biotinylated Dynorphin A 1-17 (Biotin – YGGFLRRIRPKLKWDNQ) were purchased from Anaspec (Fremont, CA). All solutions were prepared with 18.2 MΩ·cm water passed through a 0.2 μm polycarbonate Whatman filter and degassed to remove bubbles before use.
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bioRxiv - Pharmacology and Toxicology 2021Quote: Substrate was prepared by reconstituting MAPL-DNP (Anaspec) in DMSO to make 1 mM stock with gentle mixing by inversion to ensure dissolution ...
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bioRxiv - Neuroscience 2020Quote: ... 20 µM cdk5 substrate peptide (PKTPKKAKKL, Anaspec #60026-1). The reaction was allowed to proceed for 25 minutes ...
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bioRxiv - Bioengineering 2019Quote: ... along with its substrate and buffer was obtained from Anaspec, Inc ...
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bioRxiv - Biochemistry 2021Quote: ... using the fluorogenic peptide substrates suc-LLVY-amc (AnaSpec, AS-63892), boc-LRR-amc (AdipoGen ...
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bioRxiv - Neuroscience 2023Quote: ... 40µM PKC substrate peptide (amino acid sequence ERMRPRKRQGSVRRRV; AnaSpec, Fremont, CA), 50µM ATP ...
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bioRxiv - Microbiology 2020Quote: Reagents used for the peptide library experiments include: Kinase substrate library (Anaspec). Streptavidin conjugated membranes (Promega) ...
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bioRxiv - Physiology 2022Quote: ... mixed with the peptide substrate library (Anaspec #AS-62017-1 and #AS-62335), kinase assay buffer I (SignalChem ...
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bioRxiv - Cancer Biology 2023Quote: ... 20 nM of His-tagged BRD4 BD1 and 20 nM of biotinylated histone H4 K5/8/12/16(Ac) peptide (AnaSpec) were mixed in 20 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2019Quote: ... The serine/threonine peptide library (Kinase Substrates Library, Group I and Group II, Anaspec) consists of 198 peptide pools with the sequence Y-A-Z-X-X-X-X-S/T-X-X-X-X-A-G-K-K-(LC-Biotin)-NH2 ...
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bioRxiv - Immunology 2023Quote: ... 100 mM stock of dipeptidylpeptidase substrate (Acetyl-Aka-Gly-Pro-AFC) (Anaspec, CatAS-24126) was made by resuspending lyophilized substrate in DMSO ...
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bioRxiv - Biophysics 2020Quote: A super streptavidin biosensor (ForteBio, Menlo Park, USA) was coated with 15 μg/ml monomeric N-terminally biotinylated Aβ42 (AnaSpec, Fremont, USA) by overnight incubation a solution at 5 °C ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: 3CLpro activity was assayed in a 384 well plate using the 3CLpro FRET substrate (AnaSpec, Inc ...
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Long-Range Electrostatic Interactions Significantly Modulate the Affinity of Dynein for MicrotubulesbioRxiv - Biophysics 2021Quote: ... We incubated the washed beads with 0.25 mg/mL mouse monoclonal biotinylated anti-His Tag monoclonal antibody (AS-61250-BIOT, Anaspec Inc., Fremont, CA) for 2 hours at 22°C ...
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bioRxiv - Systems Biology 2022Quote: ... mixed with the peptide substrate library in solution phase (Anaspec #AS-62017-1 and #AS-62335), and incubated for 90 mins (Assay conditions for each kinase described in Table S1)18–22 ...
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bioRxiv - Biochemistry 2019Quote: ... The reaction was initiated by the injection of 5 µL of HCV NS3/4A protease substrate (AnaSpec) using a Perkin-Elmer EnVision plate reader ...
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bioRxiv - Biochemistry 2019Quote: ... 20 µM of substrate [Ac-DE-Dap(QXL520)-EE-Abu-γ-[COO]AS-C(5-FAMsp)-NH2] (AnaSpec) was serially diluted into 2x assay buffer [50 mM Tris at pH 7.5 ...
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bioRxiv - Neuroscience 2021Quote: ... the sections were incubated for 30 minutes in ACSF with A580 MMP Substrate 1 (1 μg/ml, Anaspec) at RT ...
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bioRxiv - Biochemistry 2021Quote: ... The proteolytic reaction was initiated by the injection of 5 μL of HCV NS3/4A protease substrate (AnaSpec), to a final concentration of 200 nM and kinetically monitored using a Perkin Elmer EnVision plate reader (excitation at 485 nm ...
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bioRxiv - Biochemistry 2020Quote: ... was used for detection of deacetylation activity with the acetylated peptide substrates derived from histone Bio-H4K12Ac (Anaspec #64849) and non-histone Bio-p53K382Ac (Anaspec #65046 ...
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bioRxiv - Bioengineering 2023Quote: ... MMP-13 production was measured using an enzyme-specific fluorescence substrate kit (SensoLyte 520 MMP-13 Assay Kit; AnaSpec), according to the manufacturer’s instructions.
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bioRxiv - Neuroscience 2021Quote: ... 0.05% Brij-35) and 1 µM of 520 MMP FRET substrate III (Cat. No. 60570-01; AnaSpec, San Jose, CA) was added ...
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bioRxiv - Biochemistry 2021Quote: ... a 20 μM concentration of substrate [Ac-DE-Dap(QXL520)-EE-Abu-γ-[COO]AS-C(5-FAMsp)-NH2] (AnaSpec) was serially diluted into assay buffer [50 mM Tris ...
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bioRxiv - Cancer Biology 2022Quote: ... was added to each well of a 384-well plate containing 1 μM Kemptide peptide substrate (5-FAM-LRRASLG; AnaSpec 2933), Km concentrations of ATP (5 μM ATP) ...
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bioRxiv - Microbiology 2021Quote: ... followed by the addition of 25 μL of assay buffer containing substrate (FAM-SAVLQ/SG-QXL®520, AnaSpec, Fremont, CA). The substrate was derived from the cleavage sites on the viral polyproteins of SARS-CoV ...
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bioRxiv - Microbiology 2022Quote: ... and mixed with the same volume of assay buffer containing the FRET substrate 5-FAM-ASFEGS-K(QXL520)-NH2 (AnaSpec, Fremont, CA). The cleavage site (ASFEGS ...
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bioRxiv - Biochemistry 2021Quote: ... 0.2 μM NRMT1 or 0.2 μM NRMT1+NRMT2 was incubated in wells on a 96-well plate with 40 μM of synthetic peptide substrate corresponding to the first 15 N-terminal amino acids of ZHX2 (minus the initiating methionine) (AnaSpec, Fremont, CA) in the presence of 40 μM S-adenosyl methionine (SAM) ...