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Citations for Anaspec :
1 - 50 of 51 citations for PAS Domain Containing Serine threonine Protein Kinase PASK Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2019Quote: ... The serine/threonine peptide library (Kinase Substrates Library, Group I and Group II, Anaspec) consists of 198 peptide pools with the sequence Y-A-Z-X-X-X-X-S/T-X-X-X-X-A-G-K-K-(LC-Biotin)-NH2 ...
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bioRxiv - Cell Biology 2020Quote: ... containing 1 μM JAG-1 protein (AnaSpec AS-61298, NC0243900) in a flat bottom 48-well plate (Corning 3548) ...
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bioRxiv - Biochemistry 2019Quote: ... fIX -18/+41 (the propeptide and Gla domain of factor IX, Anaspec), 1 uM ...
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bioRxiv - Microbiology 2020Quote: Reagents used for the peptide library experiments include: Kinase substrate library (Anaspec). Streptavidin conjugated membranes (Promega) ...
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bioRxiv - Cancer Biology 2023Quote: ... The kinase reaction was initiated by the addition of ATP (10 µM final) and fluorescein labeled Scrtide peptide (AnaSpec, Fremont, CA)(1 µM final) ...
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bioRxiv - Biophysics 2023Quote: ... Residual native CaM was stripped from SR by incubation with 300 nM of the M13 skeletal muscle myosin light-chain kinase CaM-binding peptide (Anaspec, Fremont, CA, USA), as described previously [35] ...
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bioRxiv - Neuroscience 2022Quote: ... solutions containing OT (ANASPEC or KS-V Peptide) or AVP (ANASPEC or KS-V Peptide) ...
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bioRxiv - Molecular Biology 2022Quote: ... Purified SIINFEKL peptide from ovalbumin protein (AnaSpec) was added to cells at 25 µg/ml for 4h ...
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bioRxiv - Biochemistry 2022Quote: ... pH 6.5) containing 25 µM each Angiotensin-I (Anaspec, Fremont, CA), Angiotensin-II (Anaspec ...
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bioRxiv - Cell Biology 2020Quote: ... the medium was replaced with MEF medium containing Aha (0.2 mM, Anaspec, #63669), instead of Met ...
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bioRxiv - Neuroscience 2023Quote: ... the pre-equilibrated hypoxic media containing the peptide Adrenomedullin (ADM) (0.5 μM, Anaspec, AS-60447) +/- 10 uM ADM22-52 (the RAMP2/3 receptor blocker (Cayman Chemical Company ...
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bioRxiv - Cell Biology 2023Quote: ... washed again 3X with PBS/1% NDS and lastly with PBS containing Hoechst33342 (AnaSpec, AS-83218) at a 1:1000 dilution ...
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bioRxiv - Cancer Biology 2023Quote: ... Me-HA was conjugated via Michael Addition with the cysteine-containing RGD peptide Ac-GCGYGRGDSPG-NH2 (Anaspec) at a concentration of 0.5 mmol/l.
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bioRxiv - Bioengineering 2023Quote: ... Me-HA was conjugated via Michael Addition with the cysteine-containing RGD peptide Ac-GCGYGRGDSPG-NH2 (Anaspec) at a concentration of 0.5Lmmol/l.
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bioRxiv - Biochemistry 2019Quote: ... MGP 1-64 (the first 64 amino acids of matrix Gla protein, Anaspec), 1 uM ...
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bioRxiv - Neuroscience 2022Quote: ... and incubated overnight at 4ºC with the primary antibody: rabbit polyclonal antibody against P2RY12 (1:250, #AS-55043A, AnaSpec Inc.). The secondary antibody was ...
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bioRxiv - Bioengineering 2024Quote: ... HAMe was conjugated via Michael Addition with the cysteine-containing RGD peptide Ac-GCGYGRGDSPG-NH2 (Anaspec, AS-62349) to obtain HAMe-RGD stock solutions used to make 15 mg/mL HA hydrogels that have a final RGD concentration of 0.15 mg/mL.
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bioRxiv - Cell Biology 2020Quote: ... Antibodies concentration: anti-Tp53 (1:1000, Anaspec, 55342); anti-g-H2AX (1:1000 ...
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bioRxiv - Cell Biology 2024Quote: ... Antibodies concentrations: anti-p53 (1:1000, Anaspec, 55342), anti TBK1 (1:1000 ...
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bioRxiv - Biochemistry 2023Quote: ... followed by HRP-conjugated secondary antibody (Anaspec, 1:2000). Antibodies were dissolved in 1X TBS with 0.05% Tween-20 ...
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bioRxiv - Immunology 2021Quote: ... splenocytes were incubated for 5 hours at 37°C in RPMI-1640 media containing 10% (v/v) bovine growth serum with 10 nM GP33-41 peptide (Anaspec) and Protein Transport Inhibitor (eBioscience ...
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bioRxiv - Plant Biology 2023Quote: ... Custom RIN4 antibody was generated by AnaSpec (Fremont, CA, USA) and used at 1:5000 dilution in 5% Milk ...
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bioRxiv - Cancer Biology 2021Quote: ... Matrix solution was mixed 12:1 (v/v) with an internal calibrant mix containing 0.08 µg/ml Angiotensin I (Anaspec, Seraing, Belgium), 0.04 µg/ml Substance P (Anaspec ...
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bioRxiv - Cancer Biology 2022Quote: ... was added to each well of a 384-well plate containing 1 μM Kemptide peptide substrate (5-FAM-LRRASLG; AnaSpec 2933), Km concentrations of ATP (5 μM ATP) ...
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bioRxiv - Microbiology 2021Quote: ... followed by the addition of 25 μL of assay buffer containing substrate (FAM-SAVLQ/SG-QXL®520, AnaSpec, Fremont, CA). The substrate was derived from the cleavage sites on the viral polyproteins of SARS-CoV ...
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bioRxiv - Microbiology 2022Quote: ... and mixed with the same volume of assay buffer containing the FRET substrate 5-FAM-ASFEGS-K(QXL520)-NH2 (AnaSpec, Fremont, CA). The cleavage site (ASFEGS ...
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bioRxiv - Neuroscience 2023Quote: ... The primary antibodies to rabbit anti-Elp1 (1:1500) (Anaspec, #AS_54494)) and rabbit anti-GFP (1:2000 ...
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bioRxiv - Cell Biology 2024Quote: ... ISCs (2,000 cells) and Paneth cells (2000 cells) were then seeded into 30 μl Matrigel containing 1 μM Jagged-1 (AnaSpec, San Jose, CA) and 10 μM Y-27632 ...
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bioRxiv - Neuroscience 2022Quote: ... Fluorescent labeling of intermediate curli was performed using a HiLyte Fluor 555 protein labeling kit (Anaspec AS-72045). An aliquot of curli or curli-HiLyte555 was thawed from −80 °C ...
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bioRxiv - Developmental Biology 2022Quote: ... followed by incubation with P2ry12 antibody (Catalog# AS-55043A, AnaSpec, Fremont, CA) overnight at 4•C and then incubated with goat anti-rabbit Alexa Fluor 488 or 647 (Thermo Fischer Scientific ...
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bioRxiv - Developmental Biology 2019Quote: ... Primary antibodies Rabbit anti-Vangl2 (1:100, Anaspec, AS-55659s, now discontinued) and Mouse anti β-II-Spectrin (1:200 ...
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bioRxiv - Developmental Biology 2019Quote: ... Western Blot was performed using 1:500 dilution of Celsr1a antibody (AnaSpec. Inc).
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bioRxiv - Molecular Biology 2019Quote: Primary antibodies used for immunoblotting were as follows: anti-IKAP (Anaspec, cat# 54494), anti-acetylated α-tubulin (Sigma ...
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bioRxiv - Neuroscience 2021Quote: ... The following primary antibodies were used: rabbit anti-P2Y12R (1:500, #55043AS AnaSpec), chicken anti-GFP-tag (1:500 ...
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bioRxiv - Cell Biology 2023Quote: ... the sections were reacted with polyclonal anti-GFP antibody (AnaSpec, Fremont, CA, US) diluted 1:500 in 3% (w/v ...
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bioRxiv - Plant Biology 2023Quote: ... the sections were reacted with polyclonal anti-GFP antibody (AnaSpec, Fremont, CA, US) diluted 1:500 in 3% (w/v ...
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bioRxiv - Neuroscience 2019Quote: ... Single cells were re-plated onto matrigel treated coverslips at low density in the presence of 10nM PACAP protein (AnaSpec) or 0.01% DMSO (Sigma) ...
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bioRxiv - Cell Biology 2020Quote: ... the membrane was incubated with the primary antibody (rabbit anti-Sarm1, 1:500, ANASPEC 55381 ...
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bioRxiv - Bioengineering 2022Quote: ... ACE2-expressing HEK293T and HEK293T control cells were lysed and the activity of the ACE protein was assessed by the SensoLyte 390 ACE2 Activity Assay Kit (AnaSpec, USA) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... One mg of each protein was labeled with a 10-fold molar excess of TMR 5-iodoacetamide (5-TMRIA; Anaspec, San Jose, CA) per cysteine (from a stock concentration of 20 mM in dimethylformamide ...
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bioRxiv - Neuroscience 2020Quote: ... and primary antibodies (rabbit anti-Iba1, 1:500 Wako #016-26461; rabbit anti-P2y12, 1:500 Anaspec #AS-55043A ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were then incubated in different mixtures of primary antibodies: rabbit anti-P2Y12R (1:500, #55043AS AnaSpec), chicken anti-GFAP (1:500 ...
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bioRxiv - Neuroscience 2023Quote: ... The following primary antibodies were used: 1) rabbit anti-pT217-tau at 1:200 (cat# AS-54968, Anaspec). The immunogen used KLH conjugated with synthetic peptides corresponding to human tau at phosphorylated threonine 217 ...
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bioRxiv - Neuroscience 2022Quote: ... Pre-treatments included mixing Aβ42-HiLyteFluor-555 with 1 μg of blocking antibodies (α-Aβ42-Ab, Anaspec, Fremont, CA) for 15 min at room temperature prior to adding viral particles or pretreating the HSV-1-gB-GFP for 30 min at room temperature with 1% NP40 to disrupt the viral envelope ...
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A Novel PHOX/CD38/MCOLN1/TFEB Axis Important For Macrophage Activation During Bacterial PhagocytosisbioRxiv - Immunology 2019Quote: ... Cells were washed thrice in PBS and incubated with the fluorescent secondary antibody plus Hoechst stain (Anaspec, AS-83218) at room temperature for 1 h ...
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bioRxiv - Neuroscience 2023Quote: ... for 30 min and incubated with blocking solution (5% normal bovine serum in PBST) for 1 h followed by rabbit anti-P2Y12 antibody (AS-55043A, AnaSpec) together with goat anti-Iba1 antibody (ab5076 ...
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bioRxiv - Neuroscience 2023Quote: ... Secondary antibodies were goat anti-rabbit (Pierce, Cat.No.31462, 1:10000) and goat anti-mouse (AnaSpec, Cat.No.28173, 1:10000). All antibodies were diluted in I-BlockTM protein-based blocking reagent (Applied Biosystems ...
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bioRxiv - Immunology 2021Quote: ... slides were incubated in blocking at RT for 1 h before overnight incubation at 4°C with the primary rabbit anti-P2Y12 receptor antibody (1:200, AnaSpec #AS-55043A) and labelling for 1 hour with the secondary antibody AF488 goat anti-rabbit ...
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Long-Range Electrostatic Interactions Significantly Modulate the Affinity of Dynein for MicrotubulesbioRxiv - Biophysics 2021Quote: ... We incubated the washed beads with 0.25 mg/mL mouse monoclonal biotinylated anti-His Tag monoclonal antibody (AS-61250-BIOT, Anaspec Inc., Fremont, CA) for 2 hours at 22°C ...
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bioRxiv - Neuroscience 2023Quote: ... + 1xPBS + .03% Triton + primary antibodies (chicken anti-Iba1: 1:1000, Synaptic Systems 234 009; rabbit anti-P2RY12: 1:500, Anaspec AS-55043A). After primary antibody incubation ...