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Citations for Anaspec :
1 - 50 of 56 citations for Myelin Basic Protein Biotin labeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... prepared from patient donors) or 200μg recombinant human myelin oligodendrocyte glycoprotein (hMOG 1–125, AS-55158-1000, AnaSpec) emulsified in complete (CFA ...
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bioRxiv - Biochemistry 2021Quote: ... a HABA biotin quantification kit (AnaSpec) was used to measure the biotin concentrations of each solution ...
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bioRxiv - Biochemistry 2019Quote: ... FITC-labeled Aβ (Anaspec) was prepared per the same protocol.
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bioRxiv - Biochemistry 2020Quote: ... Streptavidin biosensors loaded with Biotin-Aβ (Anaspec) were incubated with TREM2 ectodomain WT (1 ...
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bioRxiv - Neuroscience 2020Quote: ... or human biotin-beta-Amyloid (1-42) (AnaSpec) was dissolved in dimethyl sulfoxide (DMSO) ...
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bioRxiv - Bioengineering 2020Quote: ... 20 μg/ml biotin-4-fluorescein (AnaSpec, CA, USA) dissolved in PBS is added (step 3) ...
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bioRxiv - Biochemistry 2023Quote: ... One mg of each protein was labeled with a 10-fold molar excess of TMR 5-iodoacetamide (5-TMRIA; Anaspec, San Jose, CA) per cysteine (from a stock concentration of 20 mM in dimethylformamide ...
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bioRxiv - Neuroscience 2022Quote: ... beta-amyloid(1-40)-Lys(biotin-LC) (AS-23517, Anaspec), was diluted into assay buffer at 1 µg/mL and immobilized onto streptavidin coated biosensors (#18-5019 ...
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bioRxiv - Neuroscience 2021Quote: ... animals were immunized subcutaneously in the flanks with 400µg of myelin oligodendrocyte glycoprotein 35-55 (MOG 33-55) (MEVGWYRSPFSRVVHLYRNGK; Anaspec Inc. Catalog No. AS-60130-1) in a 200ul emulsion of Complete Freund’s Adjuvant (4mg/ml Mycobacterium tuberculosis ...
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bioRxiv - Physiology 2021Quote: Fluorescently labeled peptides were synthesized by Anaspec. The fluorescence polarization was performed as described previously by us9 ...
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bioRxiv - Bioengineering 2022Quote: ... Fluorescein-labeled SIINFEKL was purchased from Anaspec. All other chemicals were purchased from MilliporeSigma (Saint Louis ...
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bioRxiv - Neuroscience 2022Quote: ... hPTH (1-34)-Lys (Biotin) (20 μg/mL, AnaSpec, AS-23647) and Biotin (1 μg/mL,Sigma ...
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bioRxiv - Biochemistry 2019Quote: ... Pre-acetylated Biotin-Histone 4 Peptide was purchased from AnaSpec (#64989-025). Bovine serum albumin fraction V (#A7888) ...
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bioRxiv - Bioengineering 2023Quote: ... Thawed stock solutions (100 mM in DMSO) of biotin C2 maleimide (AnaSpec) were immediately added to the buffer-exchanged nanobodies to final concentration of 1 mM before the reaction mixture was nutated at 4 °C for 4 hours with a tinfoil cover to avoid light contact ...
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bioRxiv - Bioengineering 2019Quote: ... HiLyte 488-labeled Aβ (1-42) (HiLyte Aβ) and FAM-labeled scrambled Aβ (1-42) (FAM Scr Aβ) were purchased from AnaSpec (Fremont, CA). All other unspecified reagents were purchased from Sigma Aldrich (St ...
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bioRxiv - Bioengineering 2019Quote: ... HiLyte 488-labeled Aβ (1-42) (HiLyte Aβ) and FAM-labeled scrambled Aβ (1-42) (FAM Scr Aβ) were purchased from AnaSpec (Fremont, CA). All other unspecified reagents were purchased from Sigma Aldrich (St ...
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bioRxiv - Neuroscience 2020Quote: ... Fluorescent-labeled Aβ peptides (Anaspec, 60492-01, Fremont, USA) and FSB (Millipore ...
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bioRxiv - Biophysics 2023Quote: ... pre-saturation of Streptavidin with Biotin was achieved by mixing Streptavidin (AnaSpec Inc.) to Biotin (Merck Millipore ...
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bioRxiv - Biophysics 2023Quote: ... biotinylated Dynorphin A 1-17 (Biotin – YGGFLRRIRPKLKWDNQ) were purchased from Anaspec (Fremont, CA). All solutions were prepared with 18.2 MΩ·cm water passed through a 0.2 μm polycarbonate Whatman filter and degassed to remove bubbles before use.
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bioRxiv - Neuroscience 2022Quote: ... HiLyte™ Fluor 555-labeled (Cat. AS-60480-01, ANASPEC) were dissolved in DMSO to obtain a 1 mM stock ...
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bioRxiv - Neuroscience 2022Quote: Unlabeled and FAM-labeled synthetic human Aβ1-42 peptides (Anaspec) were dissolved in 1,1,1,3,3,3-hexafluoro-2-propanol (HFIP ...
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bioRxiv - Biophysics 2023Quote: ... and αS labeled with Hilyte Flour 532 succinimidyl ester (Anaspec) were used at 1-2% of final protein concentration.
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bioRxiv - Neuroscience 2023Quote: ... a fluorescently labeled form of arginine vasopressin (FAM-AVP, Anaspec) was infused in a similar way to the dextrans.
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bioRxiv - Neuroscience 2024Quote: ... HiLyte™ Fluor 647-labeled (Anaspec Inc., Cat#: AS- 64161) was resuspended to a 4µM stock in 50µl 1% Na4OH and 3950µl Corning™ Eagle’s Minimum Essential Medium (MEM ...
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bioRxiv - Cell Biology 2023Quote: ... and labeled with HiLyte647 (HiLyte Fluor 647 succinimidyl ester; Anaspec, 81256), rhodamine (5-(and-6)-carboxytetramethylrhodamine succinimidyl ester ...
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bioRxiv - Biophysics 2023Quote: ... TDP-43PrLd fluorescently labeled using Hilyte Flour 647 succinimidyl ester (Anaspec) and αS labeled with Hilyte Flour 532 succinimidyl ester (Anaspec ...
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bioRxiv - Neuroscience 2022Quote: ... r-peptide) and biotin-tagged Aβ (1–42) (Cat# AS-23523-05, 0.5 mg, Anaspec) were used to make oligomeric forms as described in our previous study (Naito et al. ...
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bioRxiv - Biophysics 2022Quote: ... αS was fluorescently labeled using HiLyte™ Fluor 405 succinimidyl ester (Anaspec). Monomeric αS in 20 mM MES buffer pH 6.0 was incubated with 3 molar excess of dye and incubated at 4 °C for 16 h ...
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bioRxiv - Microbiology 2019Quote: ... and oligosaccharides were labeled with 7-amino-1,3-naphthalenedisulfonic acid (81529, AnaSpec) prior to electrophoresis on 20% acrylamide gels ...
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bioRxiv - Neuroscience 2022Quote: ... cells were exposed to 2 μg/mL Fluo-488-labeled Aβ42 (Anaspec) and left to phagocytose for 2 h (37 °C ...
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bioRxiv - Neuroscience 2021Quote: ... HiLyte™ Fluor 488-labeled amyloid β peptide 25-35 (Anaspec, AS-633308) was prepared according to the manufacturer’s protocol ...
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bioRxiv - Biophysics 2021Quote: ... actin was labeled at Cys374 with erythrosin-5’-iodoacetamide (ErIA; AnaSpec, Fremont, CA)(11,14) ...
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bioRxiv - Immunology 2023Quote: ... 1 µL of HiLyte™ Fluor 488-labeled Amyloid-beta (1-42) (Anaspec) prepared by reconstituting 0.1mg in 50 µL of 1% NH4OH ...
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bioRxiv - Biochemistry 2022Quote: ... Various concentrations of KDM5C were reacted with 25 nM H3K4me3 (1-21)-biotin peptide (AS-64357, AnaSpec) in 50 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2020Quote: ... HiLyte Fluor 647-labelled Aβ42 (used in IP and dot blot) and Biotin-Aβ42 (used in BLI) were purchased from AnaSpec and Bachem ...
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bioRxiv - Neuroscience 2019Quote: ... recombinant SHSY5Y cells were incubated with varying concentrations of Aβ42 (HiLyte Fluor labeled Aβ42, Anaspec) (50 ...
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bioRxiv - Biochemistry 2020Quote: Stock solutions of HiLyte™ Fluor 488- labeled Human Aβ42 (Aβ, 0.1 mg; AnaSpec, USA) were prepared by dissolving the lyophilized peptide (1% NH4OH ...
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bioRxiv - Biochemistry 2020Quote: Fluorescent (HiLyte™ Fluor 488) labeled Aβ42 was purchased for cell culture assays from Anaspec (sequence ...
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bioRxiv - Physiology 2023Quote: 0.1 mg of lyophilized HiLyte Fluor 488-labeled amyloid-β1-42 (Anaspec AS-60479-01) was dissolved in 50 µL of 1% NH4OH and diluted to 0.5 mg/mL with 1xPBS before aliquoting for storage at −20°C until further use ...
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bioRxiv - Immunology 2023Quote: ... Tetanus Toxoid (TT) was labeled with APC or PE using an AnaTag labeling kit (Anaspec) according to the manufacturer’s protocol ...
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bioRxiv - Biophysics 2023Quote: αS and TDP-43PrLD were fluorescently labeled using Hilyte Flour 532/647 succinimidyl ester (Anaspec) and Hilyte Flour 647 ...
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bioRxiv - Biochemistry 2020Quote: ... All GrpE variants were labeled with DACM (N-(7-dimethylamino-4-methylcoumarin-3-yl)maleimide) (AnaSpec) and DnaK was labeled with BODIPY-fluorescein-N-(2-aminoethyl)-maleimide (Invitrogen ...
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bioRxiv - Neuroscience 2020Quote: Fluorescent Aβ42 oligomers and fibrils were prepared similarly with HiLyte Fluor 555-labeled Aβ42 (Anaspec, AS-60480-01) and mixed with non-labeled Aβ42 peptides in a 1:2 ratio.
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bioRxiv - Bioengineering 2022Quote: ... and then with tetramethylrhodamine (TAMRA)-labeled Ang II (10-8 mol/L in PBS; AS-61181, AnaSpec, Fremont, CA) for 30 min ...
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bioRxiv - Cell Biology 2023Quote: ... Each peptide is N-acetylated and C-amidated to reduce the effect of charged terminal residues. Fluorescein isothiocyanate (FITC)-labeled Tat peptide (FITC-LC-Tat, a.a. 47-57) was purchased from AnaSpec, Inc ...
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bioRxiv - Immunology 2021Quote: ... and custom Mouse amyloid-beta 42 labeled with HiLyte 488 (SQ-ANCPXXXX) were all purchased from Anaspec (Fremont, CA, USA). ACK lysing buffer (10-548E ...
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bioRxiv - Molecular Biology 2022Quote: ... Purified SIINFEKL peptide from ovalbumin protein (AnaSpec) was added to cells at 25 µg/ml for 4h ...
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bioRxiv - Molecular Biology 2019Quote: Lyophilized Aβ1-42 and HiLyte Flour 488-labeled Aβ1-42 (FITC-Aβ) (Abcam, Cambridge, UK and Anaspec Fremont, California, USA respectively) were reconstituted in 1% NH4OH to 2 mg/ml concentration and was further diluted to 1 mg/ml in 1X PBS ...
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bioRxiv - Cancer Biology 2023Quote: ... The kinase reaction was initiated by the addition of ATP (10 µM final) and fluorescein labeled Scrtide peptide (AnaSpec, Fremont, CA)(1 µM final) ...
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bioRxiv - Molecular Biology 2021Quote: ... Phagocytosis assays were done by incubating BV2 cells with 100 µM HiLyte™ Fluor 488-labeled human Aβ1-42 (Anaspec #AS-60479-01) for three hours in the presence or absence of the hybrid protein at 37°C for Aβ uptake and at 4°C for surface binding assay following Prasad and Rao (2018).