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Citations for Anaspec :
1 - 50 of 68 citations for Cyclic GMP Direct Chemiluminescent ELISA Kit 5 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... Total anti-MOG IgG was quantified by using SensoLyte Anti-Mouse MOG(1–125) IgG Quantitative ELISA Kit (Anaspec).
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bioRxiv - Cancer Biology 2022Quote: ... was added to each well of a 384-well plate containing 1 μM Kemptide peptide substrate (5-FAM-LRRASLG; AnaSpec 2933), Km concentrations of ATP (5 μM ATP) ...
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bioRxiv - Cell Biology 2022Quote: Fluorescein-5-Maleimide (AnaSpec) was attached to ubiquitin following the directions as previously described 40 ...
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bioRxiv - Microbiology 2019Quote: ... Next 5-TAMRA (4eq; Anaspec) was dissolved in 1 ml DMSO:NMP (1:1) ...
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bioRxiv - Biochemistry 2021Quote: ... and 1 mM 5-FAM-Lysine (Anaspec), prepared from a 20 mM stock in DMSO ...
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bioRxiv - Cell Biology 2023Quote: ... 5-FAM-LC-LL-37 (AS-63694, Anaspec), Crotamine (CRO01-01000 ...
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bioRxiv - Molecular Biology 2021Quote: ... After the addition of 15 µl of 5× click reaction buffer (7.5 mM sodium ascorbate [Nacalai Tesque], 0.5 mM TBTA [AnaSpec], and 5 mM CuSO4) and 1.5 µl of 10 mM Azide-PEG4-Biotin (Click Chemistry Tools) ...
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bioRxiv - Biophysics 2021Quote: ... 5-FAM fluorlabeled Crosstide was from AnaSpec (Fremont, CA). C1 peptide was from Promega (Madison ...
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bioRxiv - Biochemistry 2023Quote: ... One mg of each protein was labeled with a 10-fold molar excess of TMR 5-iodoacetamide (5-TMRIA; Anaspec, San Jose, CA) per cysteine (from a stock concentration of 20 mM in dimethylformamide ...
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bioRxiv - Biochemistry 2022Quote: ... N-methylanthraniloyl guanosine-5’-triphosphate (mant-GTP) was obtained from AnaSpec. Glutaraldehyde was purchased from Sigma-Aldrich ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: 3CLpro activity was assayed in a 384 well plate using the 3CLpro FRET substrate (AnaSpec, Inc ...
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bioRxiv - Cell Biology 2019Quote: ... differentiated SH-SY5Y cells were incubated with 5 μM TAT-TAMRA (AnaSpec) and 3 μM α-synPFF-AF488 either in the presence or in the absence of 250 μM polyP ...
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bioRxiv - Immunology 2023Quote: ... 4μM of OVA (OVA 257-264, Anaspec Inc., Cat#AS-60193-5) or gp33 (gp33-41 ...
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bioRxiv - Microbiology 2020Quote: ... Ubiquitin was further cross-linked with fluorescein-5-maleimide (Anaspec, Fremont, CA, US) and the poly-ubiquitination sample was generated following previous protocol 49 ...
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bioRxiv - Biophysics 2021Quote: ... actin was labeled at Cys374 with erythrosin-5’-iodoacetamide (ErIA; AnaSpec, Fremont, CA)(11,14) ...
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bioRxiv - Cell Biology 2019Quote: ... following the manufacturer’s instructions (SensoLyte p300 Assay kit, SensoLyte PCAF Assay kit, Anaspec) using appropriate dilutions of NDGA and anacardic acid ...
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bioRxiv - Cell Biology 2021Quote: ... and Aβ-TMR (Aβ1–42-5-tetramethyl rhodamin) suspended in 1,1,1,3,3,3-hexafluoro-2-propanol (AnaSpec). Lyophilized Aβ and Aβ-TMR were resuspended at a concentration of 5 mM in Me2SO and then diluted to a concentration of 100 μM in HEPES buffer ...
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bioRxiv - Biochemistry 2022Quote: N-terminal labeling of KSL-128114 with 5-(and-6)-carboxytetramethylrhodamine (TAMRA, Anaspec Inc.) was performed on resin ...
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bioRxiv - Biochemistry 2021Quote: ... a HABA biotin quantification kit (AnaSpec) was used to measure the biotin concentrations of each solution ...
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bioRxiv - Immunology 2022Quote: ... 2×106 splenocytes were plated in 96-well plates (Fisher, #130188) in 200 μL T-cell media and stimulated with the indicated peptide (SIINFEKL [Anaspec, #AS-60193-1] ...
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bioRxiv - Molecular Biology 2020Quote: ... Arabidopsis leaves of mature plants (5-6 weeks old) were infiltrated with either flg22 (1 μM; Anaspec) or H2O (mock ...
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bioRxiv - Biochemistry 2019Quote: ... The reaction was initiated by the injection of 5 µL of HCV NS3/4A protease substrate (AnaSpec) using a Perkin-Elmer EnVision plate reader ...
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bioRxiv - Microbiology 2023Quote: ... pestis KIM6+ was examined using membrane potential sensitive fluorophore 3,3-dipropylthiadicarbocyanine iodide [DiSC3(5)] (Anaspec Inc, USA) (33) ...
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bioRxiv - Physiology 2024Quote: ... MW 30000–50000 Da) having 1 % of the monomers modified with 5-carboxytetramethylrhodamine (TAMRA, AnaSpec; PMAO-MNP). Further functionalization was performed by incubating 1 mg of Fe with 66 µmol of 1-ethyl-3-(3-dimethylaminopropyl ...
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bioRxiv - Bioengineering 2023Quote: ... MMP-13 production was measured using an enzyme-specific fluorescence substrate kit (SensoLyte 520 MMP-13 Assay Kit; AnaSpec), according to the manufacturer’s instructions.
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bioRxiv - Biochemistry 2019Quote: ... 20 µM of substrate [Ac-DE-Dap(QXL520)-EE-Abu-γ-[COO]AS-C(5-FAMsp)-NH2] (AnaSpec) was serially diluted into 2x assay buffer [50 mM Tris at pH 7.5 ...
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bioRxiv - Biochemistry 2021Quote: ... The proteolytic reaction was initiated by the injection of 5 μL of HCV NS3/4A protease substrate (AnaSpec), to a final concentration of 200 nM and kinetically monitored using a Perkin Elmer EnVision plate reader (excitation at 485 nm ...
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bioRxiv - Bioengineering 2023Quote: ... was determined via analysis of spent culture medium (n = 5/group/time) using a commercially available FRET-based generic MMP activity kit (SensoLyte 520 Generic MMP Activity Kit Fluorimetric; Anaspec). MMP activity is represented as the concentration of MMP cleaved product (5-FAM-Pro-Leu-OH) ...
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bioRxiv - Genetics 2020Quote: ... using the SensoLyte Malachite Green Assay kit (AnaSpec) in assay buffer (MgCl2 2 mmol/L ...
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bioRxiv - Biochemistry 2023Quote: ... 5 mM of one of the glycopeptides (Muc5AC-3, Muc5AC-13, Muc5AC-3,13, Cst1.4, and Srs13.2 (Anaspec, Fremont, CA), 5 mM UDP-2-(acetylamino)-4-F-D-galactosamine disodium salt (UDP-GalNAc-F) ...
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bioRxiv - Cell Biology 2023Quote: Analyses of MMP-2 activity were performed by a fluorescence assay kit (SensoLyte® 520 MMP-2 kit, AnaSpec, San Jose, USA). The fluorescence of the substrate cleavage product was determined at excitation/emission = 490 nm/520 nm with a Perkin-Elmer spectrofluorometer [21,22].
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bioRxiv - Bioengineering 2024Quote: ... was determined via analysis of spent culture medium (n= 8-10/group) using a commercially available FRET-based generic MMP cleavage kit (SensoLyte 520 Generic MMP Activity Kit Fluorimetric, Anaspec, Fremont, CA). MMP activity is represented as the concentration of MMP cleaved product (5-FAM-Pro-Leu-OH) ...
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bioRxiv - Biochemistry 2021Quote: ... a 20 μM concentration of substrate [Ac-DE-Dap(QXL520)-EE-Abu-γ-[COO]AS-C(5-FAMsp)-NH2] (AnaSpec) was serially diluted into assay buffer [50 mM Tris ...
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bioRxiv - Neuroscience 2023Quote: ... for 30 min and incubated with blocking solution (5% normal bovine serum in PBST) for 1 h followed by rabbit anti-P2Y12 antibody (AS-55043A, AnaSpec) together with goat anti-Iba1 antibody (ab5076 ...
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bioRxiv - Microbiology 2023Quote: ... Malachite green reagent (SensoLyte MG Phosphate Assay Kit [AnaSpec]) was added to each reaction and incubated for 30 min before measuring absorbance at 660 nm ...
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bioRxiv - Microbiology 2020Quote: The SensoLyte pNPP Alkaline Phosphatase Assay Kit (AnaSpec, Fremont, CA) was used according to the manufacturer’s instruction to measure the activity of the enzyme in each supernatant fraction obtained from the osmotic shock procedure (Tris/MgSO4 wash ...
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bioRxiv - Pathology 2022Quote: MMP-12 assay kit was purchased from AnaSpec (Fremont, CA). Frozen liver tissue (50-75 mg ...
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bioRxiv - Microbiology 2022Quote: ... and mixed with the same volume of assay buffer containing the FRET substrate 5-FAM-ASFEGS-K(QXL520)-NH2 (AnaSpec, Fremont, CA). The cleavage site (ASFEGS ...
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bioRxiv - Immunology 2021Quote: ... 5×106 lung cells were re-stimulated for 3 hours (37°C, 5% CO2) with 10 nM NP366–374 and PA224-233 peptides from Anaspec (Fremont, California) in the presence of Brefeldin/A from BD Biosciences (Franklin Lakes ...
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bioRxiv - Neuroscience 2023Quote: ... The SensoLyte Red Glucocerebrosidase (GBA) Activity Assay Kit (AnaSpec, AS-72259) was used to determine enzyme activity in serum and whole tissue lysate samples ...
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bioRxiv - Immunology 2021Quote: ... a splenocyte suspension from one OT-I Rag2-/- spleen was stimulated with 10nM Ova257-264 peptide (SIINFEKL, AnaSpec, Cat no. 60193-5-ANA) for 48h and cultured for up to 10 days ...
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bioRxiv - Immunology 2022Quote: ... Miltenyi Biotech) or BMDCs were stimulated for 3 h using Ova peptide 257–264 (SIINFEKL) (Cat no. AS-60193-5, AnaSpec, Fremont, CA, USA). For measuring antigen cross presentation ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... we used a SensoLyte® HAT (p300) Assay Kit (Anaspec, Fremont, CA). Assay was conducted on a black walled 96-well petriplate (VWR ...
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bioRxiv - Neuroscience 2022Quote: ... iPSC-MGs were treated for 5 minutes with vehicle (DMSO) or 1µM of fluorescently labeled Aβ (1-40) TAMRA (human Aβ, AnaSpec # AS-60488; (Paolicelli et al., 2017)) prepared in microglia basal media with growth factors ...
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bioRxiv - Immunology 2022Quote: Elastin cleavage was examined using a SensoLyte® Green Elastase Assay Kit (AnaSpec). Porcine elastase (AnaSpec ...
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bioRxiv - Neuroscience 2019Quote: ... Gelatinase activity was measured using the SensolyteGeneric MMP colorimetric assay kit (Anaspec, Fremont, CA). Samples were run in duplicate and end-point enzymatic activity was analyzed using a glutathione reference standard.
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bioRxiv - Microbiology 2021Quote: ... Recombinant PfQC activities were determined using SensoLyte green glutaminyl cyclase activity assay kit (Anaspec). Briefly ...
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bioRxiv - Immunology 2022Quote: Generic MMP function was determined using a Generic MMP Activity Kit (AnaSpec, AS-72202) as per the kit’s instructions ...
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bioRxiv - Bioengineering 2020Quote: ACE2 activity was evaluated using SensoLyte® 390 ACE2 Activity Assay Kit (ANASPEC; cat# 72086) according to the manufacturer’s protocol ...
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bioRxiv - Genetics 2019Quote: ... separate cells were harvested and analyzed using the SensoLyte pNPP Alkaline Phosphatase Assay Kit (AnaSpec) according to manufacturer’s instructions ...