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Citations for Anaspec :
1 - 50 of 62 citations for Ceruloplasmin Colorimetric Activity Kit 2 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2019Quote: ... Gelatinase activity was measured using the SensolyteGeneric MMP colorimetric assay kit (Anaspec, Fremont, CA). Samples were run in duplicate and end-point enzymatic activity was analyzed using a glutathione reference standard.
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bioRxiv - Cell Biology 2023Quote: Analyses of MMP-2 activity were performed by a fluorescence assay kit (SensoLyte® 520 MMP-2 kit, AnaSpec, San Jose, USA). The fluorescence of the substrate cleavage product was determined at excitation/emission = 490 nm/520 nm with a Perkin-Elmer spectrofluorometer [21,22].
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bioRxiv - Microbiology 2021Quote: ... Recombinant PfQC activities were determined using SensoLyte green glutaminyl cyclase activity assay kit (Anaspec). Briefly ...
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bioRxiv - Bioengineering 2020Quote: ACE2 activity was evaluated using SensoLyte® 390 ACE2 Activity Assay Kit (ANASPEC; cat# 72086) according to the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2023Quote: ... was determined via analysis of spent culture medium (n = 5/group/time) using a commercially available FRET-based generic MMP activity kit (SensoLyte 520 Generic MMP Activity Kit Fluorimetric; Anaspec). MMP activity is represented as the concentration of MMP cleaved product (5-FAM-Pro-Leu-OH) ...
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bioRxiv - Biochemistry 2021Quote: Human ACE2 activity was evaluated using SensoLyte® 390 ACE2 Activity Assay Kit (ANASPEC; cat# 72086) according to manufacturer’s protocol ...
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bioRxiv - Cell Biology 2019Quote: Activity was determined using the SensoLyte®520 ADAM10 Activity Assay Kit (AS-72226, AnaSpec Inc, Fremont, CA), which is based on the FRET substrate 5‐ FAM/QXL™520 with excitation/emission of 490/520 nm ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: 3CLpro activity was assayed in a 384 well plate using the 3CLpro FRET substrate (AnaSpec, Inc ...
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bioRxiv - Neuroscience 2023Quote: ... The SensoLyte Red Glucocerebrosidase (GBA) Activity Assay Kit (AnaSpec, AS-72259) was used to determine enzyme activity in serum and whole tissue lysate samples ...
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bioRxiv - Bioengineering 2022Quote: ... ACE2-expressing HEK293T and HEK293T control cells were lysed and the activity of the ACE protein was assessed by the SensoLyte 390 ACE2 Activity Assay Kit (AnaSpec, USA) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: IDE activity was measure in cortical homogenates posttreatment for1 hour using the SensoLyte IDE Activity Assay Kit (AnaSpec, Inc: Cat. # AS-72231) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: Generic MMP function was determined using a Generic MMP Activity Kit (AnaSpec, AS-72202) as per the kit’s instructions ...
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bioRxiv - Bioengineering 2024Quote: ... was determined via analysis of spent culture medium (n= 8-10/group) using a commercially available FRET-based generic MMP cleavage kit (SensoLyte 520 Generic MMP Activity Kit Fluorimetric, Anaspec, Fremont, CA). MMP activity is represented as the concentration of MMP cleaved product (5-FAM-Pro-Leu-OH) ...
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bioRxiv - Microbiology 2023Quote: ... Cathepsin B activity in cytosolic fractions was measured using the SensoLyte 520 Cathepsin B Assay Kit (AnaSpec) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: Cathepsin D (CtsD) activity was measured using the SensoLyte ® 520 Cathepsin D Assay Kit (AS-72170, Anaspec) per the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... Cathepsin L (CtsL) activity was measured using the SensoLyte ® Rh110 Cathepsin L Assay Kit (AS-72217, Anaspec) per the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2022Quote: ... brain samples were analyzed using the Sensolyte 520 Neprilysin Activity Assay Fluorometric Kit (catalog number AS-72223, AnaSpec) and following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... samples were analyzed using the Sensolyte 520 Neprilysin Activity Assay Fluorometric Kit (catalog number AS-72223, AnaSpec, Fremont, CA, USA) that employs a 5-FAM/QXL FRET substrate ...
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bioRxiv - Biochemistry 2020Quote: ... were studied using a commercially available ACE2 inhibition assay according to the manufacturer’s instructions (SensoLyte® 390 ACE2 Activity Assay Kit *Fluorimetric*, AS-72086, AnaSpec, Fremont CA). Each peptide inhibitor was first tested at 4 concentrations ...
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bioRxiv - Cell Biology 2020Quote: ... The activity of human MMP1 were measured by a SensoLyte® Plus 520 MMP-1 Assay Kit (AnaSpec, San Jose, CA, USA; AS-72012) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ADAM10 activity was measured via fluorometric FRET assay following manufacturer’s instructions (AS-72226, Sensolyte 520, Anaspec) and as previously described41 ...
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bioRxiv - Biochemistry 2020Quote: ... was used for detection of deacetylation activity with the acetylated peptide substrates derived from histone Bio-H4K12Ac (Anaspec #64849) and non-histone Bio-p53K382Ac (Anaspec #65046 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: The in vitro testing in this study was performed using a standard fluorometric furin activity assay (Anaspec: Fremont, CA). This assay uses a fluorometric substrate to measure the enzymatic activity of recombinant human furin ...
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bioRxiv - Immunology 2022Quote: ... with MMP-2 (AnaSpec) and Clostridium histolyticum collagenase (ThermoFisher Scientific ...
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bioRxiv - Immunology 2019Quote: ... (2 μg/ml/peptide, ANASPEC). Results are reported as spot-forming cells (SFC ...
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bioRxiv - Microbiology 2019Quote: ... 2 μg/mL LL-37 (Anaspec), or 250 μg/mL kanamycin (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2022Quote: ... or 2 μg/mL fluorescent beta-amyloid (Anaspec). Image masks for fluorescence area and phase were generated using IncuCyte 2020C software.
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bioRxiv - Biophysics 2021Quote: ... and magainin-2 were purchased from Anaspec (Fremont, CA). All peptides were 95% or greater purity ...
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bioRxiv - Neuroscience 2023Quote: The synthetic peptide Adrenomedullin (ADM) (2 nmol, Anaspec, AS-60447) was incubated with either ddH20 (for control condition ...
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bioRxiv - Biochemistry 2023Quote: ... 300 mg of 2-Cl-Trt resin purchased from Anaspec was transferred to a 10 ml Torviq disposable reaction vessel and swelled for 1 hour in dichloromethane (DCM) ...
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bioRxiv - Cell Biology 2019Quote: ... following the manufacturer’s instructions (SensoLyte p300 Assay kit, SensoLyte PCAF Assay kit, Anaspec) using appropriate dilutions of NDGA and anacardic acid ...
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bioRxiv - Neuroscience 2020Quote: ... and 2 μl of 20% Pluronic F-127 dissolved in DMSO (AnaSpec) and diluted in HBSS containing 10mg/ml BSA (Sigma) ...
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bioRxiv - Neuroscience 2022Quote: ... cells were exposed to 2 μg/mL Fluo-488-labeled Aβ42 (Anaspec) and left to phagocytose for 2 h (37 °C ...
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bioRxiv - Evolutionary Biology 2020Quote: ... (GSHWAVGHLM; 50 µg/mL: 2[Ser]-NMC, produced in AnaSpec; San Jose, CA) overnight at 4°C before use ...
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bioRxiv - Biochemistry 2021Quote: ... a HABA biotin quantification kit (AnaSpec) was used to measure the biotin concentrations of each solution ...
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bioRxiv - Cell Biology 2021Quote: ... and Aβ-TMR (Aβ1–42-5-tetramethyl rhodamin) suspended in 1,1,1,3,3,3-hexafluoro-2-propanol (AnaSpec). Lyophilized Aβ and Aβ-TMR were resuspended at a concentration of 5 mM in Me2SO and then diluted to a concentration of 100 μM in HEPES buffer ...
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bioRxiv - Immunology 2022Quote: ... 2×106 splenocytes were plated in 96-well plates (Fisher, #130188) in 200 μL T-cell media and stimulated with the indicated peptide (SIINFEKL [Anaspec, #AS-60193-1] ...
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bioRxiv - Neuroscience 2020Quote: ... rabbit anti-Rapgef2 (NNLE-2, custom-made by Anaspec, see ref. (Jiang et al., 2017). Confocal images were obtained on a Zeiss LSM 510 confocal microscope at the National Institute of Neurological Disorders and Stroke Light Imaging Facility ...
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bioRxiv - Cell Biology 2019Quote: ... after which cells were resuspended in 2 μg/ml 7-aminoactinomycin D (7-AAD) (Anaspec). Cell viability was assessed using Gallios (Beckman Coulter ...
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bioRxiv - Cancer Biology 2022Quote: ... was added to each well of a 384-well plate containing 1 μM Kemptide peptide substrate (5-FAM-LRRASLG; AnaSpec 2933), Km concentrations of ATP (5 μM ATP) ...
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bioRxiv - Bioengineering 2023Quote: ... MMP-13 production was measured using an enzyme-specific fluorescence substrate kit (SensoLyte 520 MMP-13 Assay Kit; AnaSpec), according to the manufacturer’s instructions.
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bioRxiv - Neuroscience 2022Quote: ... and rabbit anti-Rapgef2 (NNLE-2, custom-made by Anaspec, see ref. (Jiang et al., 2017)) ...
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bioRxiv - Genetics 2020Quote: ... using the SensoLyte Malachite Green Assay kit (AnaSpec) in assay buffer (MgCl2 2 mmol/L ...
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bioRxiv - Microbiology 2023Quote: ... Malachite green reagent (SensoLyte MG Phosphate Assay Kit [AnaSpec]) was added to each reaction and incubated for 30 min before measuring absorbance at 660 nm ...
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bioRxiv - Immunology 2020Quote: ... before addition of 1 mL of vesiculation buffer (GPMV buffer plus 25 mM PFA (paraformaldehyde, Electron Microscopy Services) and 2 mM DTT (1,4-dithiothreitol, Anaspec). Cells were incubated for 1 hour at 37°C and GPMVs were collected by removing supernatant from cells.
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bioRxiv - Microbiology 2020Quote: The SensoLyte pNPP Alkaline Phosphatase Assay Kit (AnaSpec, Fremont, CA) was used according to the manufacturer’s instruction to measure the activity of the enzyme in each supernatant fraction obtained from the osmotic shock procedure (Tris/MgSO4 wash ...
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bioRxiv - Pathology 2022Quote: MMP-12 assay kit was purchased from AnaSpec (Fremont, CA). Frozen liver tissue (50-75 mg ...
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bioRxiv - Immunology 2023Quote: OT-1 T-cells were isolated from OT-1 mice by culturing OT-1 splenocytes in T-cell media supplemented with 50IU/mL IL-2 and 1 μM OVA SIINFEKL peptide (Anaspec) for 48 h ...
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bioRxiv - Biophysics 2021Quote: ... the peptides were labeled at their N-terminus with an amine-reactive environmentally sensitive fluorescent reporter, Succinimidyl 6-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)hexanoate (NBD-X, SE) (AnaSpec, Fremont, CA) [37] ...
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bioRxiv - Biophysics 2019Quote: ... was labeled at the N-terminus with succinimidyl 6-n-7-nitrobenz-2-oxa-1,3-diazol-4-yl amino hexanoate (NBD-X SE; Anaspec, Fremont, CA). Free NBD dye was removed by gel filtration using a PD-10 column (GE Healthcare Life Sciences ...