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1 - 22 of 22 citations for 7 Amino heptanoic acid ethyl ester hydrochloride since 2019

• Use of a glycan library reveals a new model for enteric virus oligosaccharide binding and virion stabilization

  Hua Lu, Mark A. Lehrman, Julie K. Pfeiffer, bioRxiv - Microbiology 2019

  Quote: ... and oligosaccharides were labeled with 7-amino-1,3-naphthalenedisulfonic acid (81529, AnaSpec) prior to electrophoresis on 20% acrylamide gels ...
• Evolutionarily ancient BAH-PHD protein mediates Polycomb silencing

  Elizabeth T. Wiles, et al., bioRxiv - Molecular Biology 2019

  Quote: H3K27me3 (amino acids 23–34) peptide was obtained from Anaspec. For NMR studies ...
• Post-ischemic ubiquitination at the postsynaptic density reversibly influences the activity of ischemia-relevant kinases

  Luvna Dhawka, et al., bioRxiv - Neuroscience 2023

  Quote: ... 40µM PKC substrate peptide (amino acid sequence ERMRPRKRQGSVRRRV; AnaSpec, Fremont, CA), 50µM ATP ...
• Compound heterozygosity of the gamma-glutamyl carboxylase mutants V255M and S300F causes pseudoxanthoma elasticum-like disease through impaired processivity

  Mark A. Rishavy, et al., bioRxiv - Biochemistry 2019

  Quote: ... MGP 1-64 (the first 64 amino acids of matrix Gla protein, Anaspec), 1 uM ...
• Identification of dendritic cell-T cell interactions driving immune responses to food

  Maria C.C. Canesso, et al., bioRxiv - Immunology 2022

  Quote: ... 1 μM of OT-II peptide (chicken OVA amino acids 323-339 - Anaspec AS-27024) or TN peptide (β-hex peptide sequence YKGSRVWLN - GenScript)27 was added to the wells ...
• Calmodulin binds to the N-terminal domain of the cardiac sodium channel Na_v1.5

  Zizun Wang, et al., bioRxiv - Physiology 2020

  Quote: Peptides were synthesized using standard 9-fluorenylmethoxy carbonyl (Fmoc) protected and activated amino acids (Anaspec, CA, USA) on prederivatized cellulose membranes (Intavis AG ...
• Conformational clamping by a membrane ligand activates the EphA2 receptor

  Justin M. Westerfield, et al., bioRxiv - Biophysics 2021

  Quote: ... the peptides were labeled at their N-terminus with an amine-reactive environmentally sensitive fluorescent reporter, Succinimidyl 6-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)hexanoate (NBD-X, SE) (AnaSpec, Fremont, CA) [37] ...
• The effect of phosphatidylserine on the membrane insertion of the cancer-targeting ATRAM peptide is defined by the non-inserting end

  V. P. Nguyen, A. C. Dixson, F. N. Barrera, bioRxiv - Biophysics 2019

  Quote: ... was labeled at the N-terminus with succinimidyl 6-n-7-nitrobenz-2-oxa-1,3-diazol-4-yl amino hexanoate (NBD-X SE; Anaspec, Fremont, CA). Free NBD dye was removed by gel filtration using a PD-10 column (GE Healthcare Life Sciences ...
• The SAGA HAT module is tethered by its SWIRM domain and modulates activity of the SAGA DUB module

  Sara T. Haile, et al., bioRxiv - Biochemistry 2022

  Quote: ... 50 nM HAT module was incubated in buffer along with 0-700 mM H3 peptide (amino acids 1-21) (AnaSpec) at 30°C in 384-well plates (Greiner Bio-One ...
• Novel regulation of the transcription factor ZHX2 by N-terminal methylation

  Meghan M. Conner, et al., bioRxiv - Biochemistry 2021

  Quote: ... 0.2 μM NRMT1 or 0.2 μM NRMT1+NRMT2 was incubated in wells on a 96-well plate with 40 μM of synthetic peptide substrate corresponding to the first 15 N-terminal amino acids of ZHX2 (minus the initiating methionine) (AnaSpec, Fremont, CA) in the presence of 40 μM S-adenosyl methionine (SAM) ...
• α-Synuclein emulsifies TDP-43 prion-like domain – RNA liquid droplets to promote heterotypic amyloid fibrils

  Shailendra Dhakal, et al., bioRxiv - Biophysics 2023

  Quote: ... and αS labeled with Hilyte Flour 532 succinimidyl ester (Anaspec) were used at 1-2% of final protein concentration.
• Higher-order assembly is a regulatory switch that promotes outer kinetochore recruitment

  Gunter B. Sissoko, et al., bioRxiv - Cell Biology 2023

  Quote: ... and labeled with HiLyte647 (HiLyte Fluor 647 succinimidyl ester; Anaspec, 81256), rhodamine (5-(and-6)-carboxytetramethylrhodamine succinimidyl ester ...
• α-Synuclein emulsifies TDP-43 prion-like domain – RNA liquid droplets to promote heterotypic amyloid fibrils

  Shailendra Dhakal, et al., bioRxiv - Biophysics 2023

  Quote: ... TDP-43PrLd fluorescently labeled using Hilyte Flour 647 succinimidyl ester (Anaspec) and αS labeled with Hilyte Flour 532 succinimidyl ester (Anaspec ...
• Distinct neurotoxic TDP-43 fibril polymorphs can be generated by heterotypic interactions with α-synuclein

  Shailendra Dhakal, et al., bioRxiv - Biophysics 2022

  Quote: ... αS was fluorescently labeled using HiLyte™ Fluor 405 succinimidyl ester (Anaspec). Monomeric αS in 20 mM MES buffer pH 6.0 was incubated with 3 molar excess of dye and incubated at 4 °C for 16 h ...
• FAIM opposes stress-induced loss of viability and blocks the formation of protein aggregates

  Hiroaki Kaku, Thomas L. Rothstein, bioRxiv - Cell Biology 2019

  Quote: ... after which cells were resuspended in 2 μg/ml 7-aminoactinomycin D (7-AAD) (Anaspec). Cell viability was assessed using Gallios (Beckman Coulter ...
• Cytoplasmic Colocalization of Granulins and TDP-43 Prion-like Domain Involves Electrostatically Driven Coacervation Tuned by the Redox State of Cysteines

  Anukool A. Bhopatkar, et al., bioRxiv - Biophysics 2021

  Quote: ... Fluorescent labeling of GRNs was performed using HiLyte™ Fluor 405 succinimidyl ester (Anaspec) or HiLyte™ Fluor 647 succinimidyl ester for FRAP studies on GRNs ...
• Cytoplasmic Colocalization of Granulins and TDP-43 Prion-like Domain Involves Electrostatically Driven Coacervation Tuned by the Redox State of Cysteines

  Anukool A. Bhopatkar, et al., bioRxiv - Biophysics 2021

  Quote: ... Fluorescent labeling of PrLD was performed using HiLyte™ Fluor 647 succinimidyl ester (Anaspec) using a similar protocol as described above for GRNs.
• α-Synuclein emulsifies TDP-43 prion-like domain – RNA liquid droplets to promote heterotypic amyloid fibrils

  Shailendra Dhakal, et al., bioRxiv - Biophysics 2023

  Quote: αS and TDP-43PrLD were fluorescently labeled using Hilyte Flour 532/647 succinimidyl ester (Anaspec) and Hilyte Flour 647 ...
• Heme oxygenase-1 affects cytochrome P450 function through the formation of heteromeric complexes: Interactions between CYP1A2 and heme oxygenase-1

  J. Patrick Connick, et al., bioRxiv - Biochemistry 2020

  Quote: ... 7-ethoxyresorufin was purchased from Anaspec (Fremont, CA), and cytochrome c was obtained from Sigma (St ...
• Non-additive effects of changing the cytochrome P450 ensemble: incorporation of CYP2E1 into human liver microsomes and its impact on CYP1A2

  Nadezhda Y. Davydova, et al., bioRxiv - Biochemistry 2019

  Quote: ... 7-ethoxyresorufin (ERR) was from AnaSpec (San Jose, CA, USA). 7-etoxy-4-cyanocoumarin (CEC) ...
• A stutter in the coiled coil domain of E.coli co-chaperone GrpE connects structure with function

  Tulsi Upadhyay, Vaibhav V Karekar, Ishu Saraogi, bioRxiv - Biochemistry 2020

  Quote: ... All GrpE variants were labeled with DACM (N-(7-dimethylamino-4-methylcoumarin-3-yl)maleimide) (AnaSpec) and DnaK was labeled with BODIPY-fluorescein-N-(2-aminoethyl)-maleimide (Invitrogen ...
• SARS-CoV-2 envelope-protein corruption of homeostatic signaling mechanisms in mammalian cells

  Tobias Schulze, et al., bioRxiv - Pathology 2021

  Quote: ... The membrane potential of HEK293 cells was examined by loading the cells for 15 min with one of the two voltage sensitive dyes DiBAC4(3) (Bis-(1,3-Dibutylbarbituric Acid)Trimethine Oxonol) (AnaSpec, Fremont, CA, USA) or FluoVolt™ (ThermoFisher Scientific ...