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Citations for SignaGen Laboratories :
1 - 45 of 45 citations for Mouse TXNDC8 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... 293T cells were transiently co-transfected with DNA from the lentiviral pLKO.1-puro shRNA plasmid and psPAX2 and pMD2.G packaging plasmids using LipoD293™ Transfection Reagent (SignaGen Laboratories.) Conditioned media containing virions was harvested after 24 and 48 hours ...
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bioRxiv - Immunology 2023Quote: ... Lenti-X cells were transfected with a pSICO scramble non-targeting shRNA construct and pSICO Srsf7 shRNA constructs targeted at exon 3 and exon 4 of Srsf7 using Polyjet (SignaGen Laboratories). Virus was collected 24 and 48 h post transfection ...
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bioRxiv - Neuroscience 2020Quote: pCAG-eCas9-GFP-U6-Rpsa-gRNA plasmid was transfected into mouse Neuro-2a cells using PolyJet transfection reagent (SignaGen Laboratories), and Genomic DNA was isolated and subjected to PCR to amplify the 433 bp fragment containing gRNA target sequence using Q5 High Fidelity DNA polymerase (NEB ...
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bioRxiv - Cancer Biology 2022Quote: ... Lentivirus containing supernatants were produced in packaging (293T) cells transfected with individual elF4B shRNAs or the pLKO.1 eGFP positive control using LipoD293™ transfection reagent (SignaGen Laboratories; Cat# SL100668) at a 1:2.5 DNA:Lipo ratio (2.5ug transfer plasmid + 2.5ug psPAX2 + 1.0ug pMD2.G and 15ul lipoD per plate) ...
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bioRxiv - Cancer Biology 2023Quote: ... packaging plasmids psPAX2 and enveloped plasmids pMD2.G (a gift from Dr. Maciej Olszewski) into HEK-293T cells using lipoD293 (SignaGen Laboratories) as transforming reagent ...
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bioRxiv - Cell Biology 2022Quote: ... the plasmids were transfected using LipoJet (SignaGen Laboratories, Frederick, MD, USA). To construct a stable cell line ...
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bioRxiv - Biophysics 2019Quote: ... and then were transfected with plasmids using PolyJet Reagent (Signagen Laboratories) according to manufacturer’s instruction ...
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bioRxiv - Neuroscience 2023Quote: ... wild type or phosphomimetic tau plasmids using PolyJet (SL100688, SignaGen Laboratories) according to manufacturer’s instructions ...
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bioRxiv - Biophysics 2019Quote: ... and transfected with the indicated plasmids using GenJet (SignaGen Laboratories, Rockville, MD) transfection reagent according to the manufacturer instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... plasmids were incubated with 3 μl CalFectin (SignaGen laboratories, Rockville, MD, USA) in Opti-MEM (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2021Quote: ... Transient transfection with plasmids was performed using GenJet version II (SignaGen Laboratories), and transfection with poly(I:C ...
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bioRxiv - Molecular Biology 2021Quote: Plasmid transfections were performed using GenJet In Vitro DNA Transfection Reagent (SignaGen Laboratories). To overexpress NORAD ...
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bioRxiv - Genomics 2022Quote: ... plasmid DNA mixtures and 50 µl of LipoD293 transfection reagent (SignaGen Laboratories, SL100668) were mixed in 250 µl KO-DMEM at RT ...
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bioRxiv - Neuroscience 2023Quote: HEK293T cells were transfected with indicated plasmids with GenJet Transfection Reagent (SignaGen Laboratories), and at 48 hr cells were lysed in ice-cold RIPA buffer ...
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bioRxiv - Microbiology 2021Quote: ... the corresponding plasmids were transfected using Poly Jet™ transfection reagent (SignaGen Laboratories, SL100688) after the 293T cells had been grown to 80% confluence for 18-24 h before treatments ...
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bioRxiv - Microbiology 2022Quote: ... All plasmid transfections were performed by LipoD293 In Vitro DNA transfection reagent (SignaGen Laboratories) as recommended.
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bioRxiv - Cancer Biology 2020Quote: ... Mutant plasmids were directly transfected into cell lines using GeneJet transfection reagent (SignaGen Labs) or packaged in 293T for lentiviral infection.
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bioRxiv - Systems Biology 2023Quote: ... 15 μg of Strep-tagged plasmids was combined with PolyJet Transfection Reagent (SignaGen Laboratories) at a 1:3 μg:μl ratio of plasmid:transfection reagent ...
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bioRxiv - Molecular Biology 2020Quote: ... then transfected with 1ug of Tau mutant plasmids using PolyJet reagent (SL100688, SignaGen Laboratories, MD). Cells were collected 25 hours after transfection ...
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bioRxiv - Genomics 2021Quote: ... plasmids into 4 million HEK293T cells in a T25 flask with PolyJet (SignaGen Laboratories, SL100688). Media was replaced 12 h after transfection ...
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bioRxiv - Cell Biology 2023Quote: Plasmids were transfected into 40-50% confluent cells using PloyJet reagent (SignaGen Lab MD, USA). siRNAs were transfected with the siRNA transfection reagent INTERFERin® (Polyplus ...
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bioRxiv - Genetics 2021Quote: ... then the cells were transfected with plasmids using a LipoD293 DNA transfection reagent (SignaGen Laboratories, SL100668) at 24–48 hours before the imaging studies ...
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bioRxiv - Molecular Biology 2020Quote: Plasmid transfection was carried out using LipoD293™ In Vitro DNA Transfection Reagent (SignaGen Laboratories, SL100668) following manufacture's protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... packaging plasmid (Δ8.9) and envelope (VSVG) vectors using Lipo293T DNA in vitro transfection reagent (SignaGen Laboratories) according to the manufacture’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... packaging plasmid (Δ 8.9) and envelope (VSVG) vectors using Lipo293T DNA in vitro transfection reagent (SignaGen Laboratories) according to the manufacture’s protocol ...
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bioRxiv - Molecular Biology 2019Quote: ... packaging plasmid (Δ8.9) and envelope (VSVG) vectors using Lipo 293T DNA in vitro transfection reagent (SignaGen Laboratories) according to the manufacture’s protocol ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were transfected with plasmid DNA using PolyJet DNA In Vitro Transfection Reagent (Signagen Laboratories, Gaithersburg, USA) according to manufacturer’s instructions.
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bioRxiv - Cancer Biology 2021Quote: ... Transient transfections of plasmids were performed by using LipoD293™ In Vitro DNA Transfection Reagent (SignaGen Laboratories). Transient transfections of SMARTPool SGK3 siRNA (L-004162-00-0005 ...
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bioRxiv - Systems Biology 2020Quote: ... Total plasmid was normalized to 15 μg with empty vector and complexed with PolyJet Transfection Reagent (SignaGen Laboratories) at a 1:3 μg:μl ratio of plasmid to transfection reagent based on manufacturer’s recommendations ...
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bioRxiv - Genomics 2021Quote: ... lentivirus packaging plasmids into 8 million HEK293T cells in a 10-cm dish with PolyJet (SignaGen Laboratories, SL100688). For each individual sgRNA ...
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bioRxiv - Cell Biology 2020Quote: ... Total plasmid was normalized to 15 μg with vector DNA and complexed with PolyJet Transfection Reagent (SignaGen Laboratories) at a 1:3 μg ...
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bioRxiv - Systems Biology 2023Quote: ... Total plasmid was normalized to 15 μg with empty vector and complexed with PolyJet Transfection Reagent (SignaGen Laboratories) at a 1:3 μg:μl ratio of plasmid to transfection reagent based on manufacturer’s recommendations ...
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bioRxiv - Cancer Biology 2023Quote: ... and envelop plasmids pMD2.G (a gift from Dr. Maciej Olszewski) into HEK-293T cells using lipoD293 (SignaGen Laboratories) as transforming reagent ...
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bioRxiv - Cancer Biology 2023Quote: ... COMMA1D cells were then transfected with INSR knock-out plasmid using 15 µL PolyJet transfection reagent (SignaGen Laboratories #SL100688). Following transfection ...
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bioRxiv - Molecular Biology 2021Quote: ... U2OS cells were transfected with the appropriate amount of DNA plasmid according to the manufacturer’s protocol using GenJet U2OS DNA in vitro transfection reagent (SignaGen Laboratories) for 18 to 24 hrs.
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bioRxiv - Molecular Biology 2022Quote: ... Transfection of HEK293T cells was performed by mixing plasmid DNA with PolyJet™ In Vitro DNA Transfection Reagent (SignaGen Laboratories) and incubation with cells for 3 h ...
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bioRxiv - Molecular Biology 2023Quote: ... U2OS and HEK cells were transfected with the appropriate amount of DNA plasmid according to the manufacturer’s protocol using GenJet U2OS or Lipo293T DNA in vitro transfection reagent (SignaGen Laboratories) for 18 to 24 hrs.
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bioRxiv - Genetics 2021Quote: ... plasmids for either ABCC5 or ABCC12 constructs were transfected into cell lines using PolyJet transfection reagent (SignaGen Laboratories, cat. number SL100688). Previously ...
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bioRxiv - Biochemistry 2020Quote: HeLa cells were transfected with a 1:1:1 mix of gRNA1 and gRNA2 vectors together with the donor plasmid using PolyJet transfection reagent (SL100688, SignaGen Laboratories) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... The SH-SY5Y neuroblastoma cells were transfected with 10 ug of plasmid DNAs when 60% confluence in 100-mm dishes using transfection reagents GenJet II (SignaGen Laboratories, Rockville ...
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bioRxiv - Synthetic Biology 2022Quote: ... HEK293T were transfected with PB-NnSULT1C1 (100 ng) and Piggybac transposases plasmids (20 ng) with Polyjet In Vitro DNA Transfection Reagent (SignaGen Laboratories). 1 μg/mL puromycin was added to culture medium from Day 2 to Day 7 for selecting cells with genomic integration of NnSULT1C1 ...
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bioRxiv - Genomics 2023Quote: ... packaging plasmids were cotransfected into 8 million HEK293T cells in a 10-cm dish supplemented with 36 μl PolyJet (SignaGen Laboratories, SL100688). The medium was replaced 12 hours after transfection and harvested every 24 hours thereafter for a total of three harvests ...
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bioRxiv - Genomics 2023Quote: ... we transfected two million MCF7-dCas9/RT cells with 2000 ng of ngRNA/pegRNA plasmid containing an EGFP marker using PolyJet (SignaGen Laboratories, SL100688). Five days after transfection ...
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bioRxiv - Neuroscience 2024Quote: ... AAV plasmids containing the target genes were co-transfected with pAAV2 (pACG2)-RC triple mutant (Y444, 500, 730F) and the pHelper plasmid (StrateGene) into HEK293T cells by the PolyJet (SignaGen Laboratories, SL100688) transfection reagent ...
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bioRxiv - Cell Biology 2020Quote: ... and 1 μg of a gel-purified mutagenic primer targeting mouse rs11031006 (5’-CTGGAATTTAATATTGCTCTGCCCTGTGATATTTATTTCAAGGTTAGTAGAAATGTAGCTACCTCCTGTAATGACAAATGA-3’) using PolyJet In Vitro DNA Transfection Reagent (SignaGen Laboratories). At 18 hours post transfection ...