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Citations for abcam :
1 - 50 of 61 citations for Yeast Extract since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: Yeast nuclei were prepared with reagents from the Yeast nuclei isolation kit (Abcam 206997, Cambridge, United Kingdom), unless noted otherwise ...
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bioRxiv - Cell Biology 2019Quote: ... and yeast ADH1 (1:1,000--∼40kDa) (Abcam:ab34680) as a loading control ...
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bioRxiv - Cancer Biology 2020Quote: ... acid extracts were made following manufacturer (Abcam) recommendations ...
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bioRxiv - Molecular Biology 2022Quote: The Mitochondrial Yeast Isolation kit and protocol (ab178779; Abcam) were used to fractionate yeast cells by differential centrifugation ...
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bioRxiv - Immunology 2022Quote: ... MCF-7 nuclear extracts (5 μg; ab14860, Abcam) were treated with either S (0.01-300 nM ...
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bioRxiv - Microbiology 2021Quote: ... or mouse anti-PGK (yeast phosphoglycerate kinase; Abcam, 1:10,000) were used for immunoblotting along with sheep anti-mouse-HRP NXA931V secondary antibody (GE Healthcare ...
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bioRxiv - Molecular Biology 2021Quote: Nuclear RNA was obtained using the Yeast Nuclei Isolation kit (Abcam) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... The extracts were bound with anti-Chd4 (ab70469, Abcam) for 2 hours at 4°C in the presence of RNase inhibitor ...
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bioRxiv - Genomics 2021Quote: ... Chromatin extracts were incubated with anti-H3 (abcam, ab1791), anti-H3K9me3 (abcam ...
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bioRxiv - Molecular Biology 2023Quote: ... protein extract was processed according to manufacturer’s specifications (Abcam). See further details in the Supplemental Methods,
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bioRxiv - Molecular Biology 2020Quote: ... cell extracts were incubated with anti-HA antibody (Abcam ab9110) for 1h at 4℃ with gentle mixing ...
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bioRxiv - Genetics 2021Quote: ... Cell extracts were coimmunoprecipitated with Anti-TET1 antibody (Abcam, ab191698), Anti-KAT3B/p300 antibody (Abcam ...
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bioRxiv - Cancer Biology 2022Quote: ... extracts were made using the Histone Extraction Kit (Abcam #ab113476). For total protein ...
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bioRxiv - Cell Biology 2023Quote: ... and nuclear extracts was performed using a kit (ab109179, Abcam), according to manufacturer’s instructions ...
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bioRxiv - Systems Biology 2022Quote: ... and the nuclear extracts were isolated using Nuclear Extraction Kit (Abcam) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... Metabolites extracts were then used in fluorometric cysteine assay kit (Abcam).
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bioRxiv - Cancer Biology 2023Quote: ... Extracts were incubated with 5 ug of anti-DHX9 (Abcam, ab26271) or anti-IgG (Cell Signaling ...
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bioRxiv - Molecular Biology 2022Quote: Protein extracts were obtained by lysis of cells in 1xRIPA buffer (Abcam) containing complete Mini ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... Nuclear protein extracts were prepared from dissected brains following manufacturer’s instructions (Abcam Nuclear Extraction Kit ...
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bioRxiv - Genetics 2021Quote: Nuclear extract was prepared from about 108 cells using Chromatin Extraction kit (Abcam). Extracts were immunoprecipitated with rabbit anti-HA (Sigma ...
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bioRxiv - Cell Biology 2024Quote: ... Histone-enriched (acidic) proteins extracts were prepared using Histone Extraction Kit (Abcam ab113476) following manufacturer instructions ...
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bioRxiv - Genetics 2022Quote: ... yeast SUMO was detected with rabbit polyclonal anti-Smt3 antibody (1:1000, Abcam Cat# ab14405, RRID:AB_301186), followed by incubation with goat anti-mouse IgG conjugated to HRP (1:2000 ...
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bioRxiv - Neuroscience 2020Quote: ... and equal amounts of extract were immunoprecipitated with 0.5ug rabbit anti-GFP (Abcam, Ab290) overnight with rocking at 4°C ...
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bioRxiv - Genetics 2019Quote: ... extracts were incubated overnight at 4°C with 10ul of H3K9me2 antibody (ab1220, Abcam). DNA was extracted by phenol-chloroform and ethanol precipitated.
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bioRxiv - Synthetic Biology 2021Quote: ... Histones were analyzed by western blotting with antibodies against fission yeast-specific phosphoS129 of H2A (ab17353, Abcam), H2A.W.6p ...
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bioRxiv - Microbiology 2020Quote: ... Capsid p24 was measured in cell extracts and supernatants using SimpleStep ELISA (ABCAM, Cambridge, UK) and ADVIA Centaur HIV Ag/Ab Combo ELISA (Siemens Healthcare Diagnostics ...
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bioRxiv - Neuroscience 2022Quote: ... and equal amounts of extract were immunoprecipitated with 0.5 ug rabbit anti-GFP (Abcam, Ab290) overnight with rocking at 4°C ...
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bioRxiv - Molecular Biology 2023Quote: ... Extracts were split equally and incubated with 2.5 μl anti-H3 (2.5 μg; Abcam, 1791) 2.5 μl anti-SUZ12 (Cell Signaling ...
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bioRxiv - Biochemistry 2023Quote: ... The supernatant was collected as whole cell extract and incubated with anti-RAD53 (Abcam, ab104232) pre-coated Dynabeads Protein G (Invitrogen ...
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bioRxiv - Microbiology 2020Quote: ... Czech Republic) or mouse monoclonal antibody against yeast phosphoglycerate kinase PGK1 (anti-PGK1, dilution 1:5,000, Abcam, ab113687) as a loading control ...
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bioRxiv - Cell Biology 2020Quote: ... Equal micrograms of protein extracts were mixed with 2 µg of Rabbit anti-mCherry antibody (Abcam) for 6 h at 4°C under end-over-end rotation ...
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bioRxiv - Cancer Biology 2022Quote: ... and 1mg of nuclear extract was incubated with the indicated antibodies: 2.5ug of BRD4 (Abcam; ab128874), 5ug of MED1 (Bethyl Laboratories ...
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bioRxiv - Cancer Biology 2020Quote: Citrullinated proteins were immunoprecipitated from whole cell extracts with an anti-pan-citrulline antibody (Abcam, ab6464). Samples were concentrated on Amicon Ultra 0.5 mL columns (cutoff ...
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bioRxiv - Cancer Biology 2022Quote: Nuclear extracts from 5*10^6 cells were collected using a Nuclear Extraction Kit (Abcam, ab113747) according to the manufacturer’s instructions ...
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Activation of endoplasmic reticulum stress via clustering of the inner nuclear membrane protein SUN2bioRxiv - Cell Biology 2022Quote: ... 1mg of extract was then incubated with 2μg of α-GRP78/BiP rabbit polyclonal antibody (Abcam, ab21685) or 2μg normal rabbit IgG antibody (Cell Signaling ...
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bioRxiv - Biochemistry 2023Quote: ... After a period of 24h nuclear extracts (∼80 μL) were generated using a kit from Abcam (ab113474). Resultant nuclear extracts were aliquoted into single-use tubes (∼10 μL each at ∼1 mg/mL ...
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bioRxiv - Biochemistry 2023Quote: ... membrane extracts were incubated for 1h at 4°C with an anti-NDUFS3 primary antibody (ab14711; abcam) against the matrix arm of complex I from C ...
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bioRxiv - Biochemistry 2021Quote: HDAC activity of HCT116 nuclear extract was assayed using the Fluorometric HDAC Activity Assay kit from Abcam (ab156064) according to manufacturer protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... Aliquots of cytoplasmic and nuclear extracts were analyzed via immunoblot for biotinylation efficiency using streptavidin-HRP (Abcam, ab7403) and for miniTurbo–TAF protein expression using a rat anti-HA-HRP antibody (3F10 ...
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bioRxiv - Cell Biology 2021Quote: ... The fixed cells were washed three times with water and then partially lysed with a yeast nuclei isolation kit (Abcam 206997) using the following modified protocol ...
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bioRxiv - Developmental Biology 2021Quote: ... Immunoprecipitations from Xenopus egg extracts were performed as described below for mass spectrometry using rabbit anti-Yap (ab62752, Abcam) or rabbit anti-RIF antibodies ...
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bioRxiv - Cell Biology 2020Quote: ... Extracts were supplemented with cycloheximide (100 µg/mL) and where applicable with the Wee1/Myt1 inhibitor PD0166285 (Abcam, ab219507) or okadaic acid (EMD Millipore ...
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bioRxiv - Cell Biology 2020Quote: ... Endogenous SETD2 was immunoprecipitated overnight from 500-1000 µg cell extract using anti-SETD2 antibodies (Abcam, or Sigma-Aldrich) and protein A/G agarose beads (Pierce) ...
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bioRxiv - Molecular Biology 2022Quote: ... Diluted nuclear extracts were incubated with anti-RNA polymerase II CTD repeat YSPTSPS antibody (8WG16)-ChIP grade (Abcam, ab817) overnight at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: ... Whole-cell extracts (1 mg) were incubated (4°C for 1 hour) with 0.12 µl of anti-Rad51 polyclonal antibody (Abcam) for Rad51 immunoprecipitation or with 1µg of anti-FLAG monoclonal (Sigma ...
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bioRxiv - Cell Biology 2020Quote: ... murine RNAase inhibitor and 1 mg/ml yeast tRNA) and incubated with primary antibodies (Synaptic systems, 188 011, 314 006, 135 304, 106003, 131 005; Abcam, ab5392) in blocking buffer for 1 h at room temperature ...
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bioRxiv - Molecular Biology 2020Quote: ... Immunoprecipitations were performed with 1000 µg of extract and a 25 µl slurry of pan-mouse Dynabeads bound to anti-Myc (Abcam) or anti-V5 (Thermo-Fisher ...
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bioRxiv - Microbiology 2021Quote: Culture media and cellular extracts were collected and centrifuged in preparation for LDH activity quantitation with a commercially available kit (#ab102526, Abcam). LDH activity was measured by a microplate reader (Tecan Infinite M200 Pro ...
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bioRxiv - Molecular Biology 2022Quote: ... A TET activity assay was also performed on nuclear extracts using a fluorometric commercial assay (TET Hydroxylase Activity Quantification Kit, catalog# ab156913; Abcam) and microplate fluorometer (Synergy H1 ...
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bioRxiv - Molecular Biology 2022Quote: A DNMT activity assay was performed on nuclear extracts using a commercial assay (Colorimetric DNMT Activity Quantification Kit, catalog# ab113467; Abcam) and microplate spectrophotometer (Synergy H1 ...