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Citations for abcam :
1 - 50 of 1158 citations for Siglec 6 Human HEK 293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2021Quote: ... HEK-293 cells were cultured and treated with 2 µg/mL of tunicamycin (ab120296, Abcam) for 6 hours ...
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bioRxiv - Developmental Biology 2023Quote: ... rabbit anti- Siglec-F (1:200, Abcam), rabbit anti-ADRP (1:200 ...
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bioRxiv - Bioengineering 2019Quote: Extracellular domain Fc fusions for human CTLA4 (CTLA4-Fc) and ErbB2 (ErbB2-Fc) were purchased from Abcam (ab180054 and ab168896). These ligands were biotinylated by the EZ-link® Sulfo-NHS-LC-Biotin according to the manufacturer’s protocol (Thermo Scientific) ...
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bioRxiv - Immunology 2024Quote: ... CoMiX-Fc were detected with a goat anti-human IgG Fc HRP pAb (Abcam, #ab97225), whereas CoMiX-FHR4 molecules and VHH controls were detected with a mouse anti-His HRP-conjugated mAb (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2023Quote: ... Commercially obtained human CTSL (Cathepsin L/MEP) knockout HEK-293T cells (abcam; ab266521) and human wild-type HEK293T cells (abcam ...
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bioRxiv - Neuroscience 2021Quote: ... On DIV14 cells were treated with human Fc (Abcam #90285) or recombinant mouse Sema3F-Fc fusion protein (R&D Systems #3237-S3 ...
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bioRxiv - Immunology 2020Quote: ... HRP conjugated goat anti-human IgG Fc was from Abcam, Cambridge ...
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bioRxiv - Microbiology 2023Quote: The Fc portion of human Immunoglobulin G (1 mg, Abcam) was labelled with biotin (Thermo Scientific EZ-Link Sulfo-NHS-Biotin ...
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bioRxiv - Neuroscience 2023Quote: ... cultures were treated with purified Fc from human IgG (Abcam #ab90285) or recombinant mouse Sema3F-Fc fusion protein (R&D Systems #3237-S3 ...
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bioRxiv - Cell Biology 2021Quote: ... bound IRE1α LD-Fc was transferred after washes to a PVDF membrane and detected with anti-human Fc antibody (ab977225, Abcam), ECL solution (Thermo-Fisher ...
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bioRxiv - Immunology 2024Quote: ... 100 ng/well goat anti-human IgG Fc (Abcam, Cambridge, UK, #ab97221) or rabbit anti-His (Bethyl ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Levels of IL-6 in supernatants were determined using human IL-6 ELISA kit (Abcam). The range for IL-6 detection was 7.8-500 pg/mL ...
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bioRxiv - Immunology 2021Quote: ... the antigens and a capture anti-human IgE (Mouse anti-Human IgE Fc) (ref. ab99834, Abcam PLC, Cambridge, UK) were coupled to magnetic MagPlex® 6.5μm COOH-microspheres (Luminex Corporation ...
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bioRxiv - Immunology 2020Quote: ... Recombinant human coronavirus SARS-CoV-2 spike glycoprotein S1 (Fc Chimera) (ab272105, Abcam) was used as positive control (loaded 2.4 ug/lane) ...
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bioRxiv - Neuroscience 2020Quote: ... ephrin-A5 conjugated to human FC (ephrinA5-hFC, R&D) or hFC control (R&D) was pre-clustered with goat anti- human antibody (Abcam) for one hour at 37°C before being added to transfected HEK cells (5 μg /ml ...
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bioRxiv - Immunology 2024Quote: ... CoMiX-Fc molecules were revealed with goat anti-human IgG AF647-conjugated antibody (Abcam), which cross-reacts with mouse IgGs ...
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bioRxiv - Immunology 2020Quote: ... followed by incubation with PE-labeled goat anti-human IgG-Fc antibody (1:5000; Abcam) for 30 min and analyzed by flow cytometry.
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bioRxiv - Microbiology 2022Quote: ... cells were dissociated and incubated with recombinant human ACE2-Fc(IgG) (1 mg/ml) (Abcam ab273687) at a dilution of 1:32000 for 30 minutes ...
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bioRxiv - Microbiology 2023Quote: ... and directly incubated with the secondary antibody using Goat Anti-Human IgG Fc (HRP) preadsorbed (Abcam). After washing five times with Tris-buffered saline and Tween 20 ...
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bioRxiv - Immunology 2020Quote: ... after twice washes then PE-labeled goat anti-human IgG-Fc antibody was added (1:5,000; Abcam) for 30 min ...
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bioRxiv - Microbiology 2023Quote: ... The secondary antibodies included: (1) goat anti-human IgG Fc (HRP) pre-adsorbed (catalog no. ab98624; Abcam); (2 ...
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bioRxiv - Cancer Biology 2020Quote: ... mouse anti-human FOXP3 (Abcam, clone 236A/E7, 1:600, pH 6 retrieval), rabbit anti-human CD4 (Abcam ...
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bioRxiv - Genetics 2023Quote: ... 6) Arabidopsis-specific H2A.Z antibody [22] and human-specific H2A.Z antibody (Abcam, ab188314) were added to the diluted lysates at a final concentration of ∼2 μg/ml and incubated overnight at + 4°C with rocking ...
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bioRxiv - Cell Biology 2021Quote: ... Plates were washed again with PBSt and incubated with an anti-human Fc HRP-conjugated antibody (ab977225, Abcam). Development was performed with TMB solution and terminated with Stop solution (Biolegend) ...
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bioRxiv - Immunology 2022Quote: ... 3 ml of the adjuvant goat IgG anti-human IgM Fc (5 ng/ml) (by ABCAM, Cambridge, UK) was dissolved in human albumin and phosphate-buffered normal saline added to the remainder of the sediments and packaged in 1 ml vials of injectable solution with labels (See Supplement ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were then incubated with goat F(ab’)2 anti-Human IgG Fc-FITC antibody (Abcam, Cambridge, MA) for 30 minutes in the dark on ice ...
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bioRxiv - Cancer Biology 2019Quote: ... or Fc-iNKG2D.AF and bound MicAbody detected with an HRP-conjugated mouse-anti-human kappa chain antibody (Abcam #ab79115). Affinity of both monomeric and antibody-fused ULBP2 variants was also determined by Octet analysis as described above.
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bioRxiv - Cancer Biology 2022Quote: ... and 1.5 nM followed by detection with a goat anti-human Fc FITC secondary antibody (Abcam ab97224, Cambridge, UK) at 1:200 and fixation in 4% formalin ...
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bioRxiv - Immunology 2020Quote: ... the membranes were washed with TBST and incubated with HRP-conjugated Goat-anti-human Fc antibody (Abcam, ab99759, 1:10000) for 1 h at RT ...
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bioRxiv - Immunology 2021Quote: ... the membranes were washed with TBST and incubated with HRP-conjugated Goat-anti-human Fc antibody (Abcam, ab99759, 1:10000) for 1 h at RT ...
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bioRxiv - Immunology 2022Quote: Recombinant human peroxiredoxins 1 and 6 (PRX1, PRX6) were purchased from Abcam (Abcam PLC, Cambridge, UK), while recombinant human β-defensin-1 and 2 (HBD-1 ...
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bioRxiv - Cell Biology 2022Quote: Mouse monoclonal antibodies to Siglec-1 (Hsn 7D2) and rabbit polyclonal antibodies against cofilin and phosphor-cofilin (phospho S3) were obtained from Abcam. Rabbit antibodies against pERM (48G2 ...
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bioRxiv - Immunology 2022Quote: Binding of TIM-4 to infected cells was tested using a TIM-4 Fc chimera composed of the extracellular domain of TIM-4 fused to the N-terminus of the Fc region of human IgG (Abcam, Waltham, MA). One μg of TIM-4 in PBS containing 1% BSA was incubated on ice for 30 minutes with 105 washed CD4 T cells infected as indicated above with SIVGAGGFP ...
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bioRxiv - Immunology 2023Quote: ... Fc-CD19-FITC (abcam, ab246020), anti-TIM-3-PE (BD ...
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bioRxiv - Cell Biology 2019Quote: ... Same experiment was also performed blocking monocyte-derived macrophages at RT for 15 min with 10 μg/ml of mAb α-Siglec-1 7–239 (Abcam), IgG1 isotype control (BD Biosciences ...
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bioRxiv - Cell Biology 2022Quote: ... interleukin 6 (IL-6, Abcam#ab229434) and growth differentiation factor 15 (GDF15 ...
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bioRxiv - Cell Biology 2020Quote: ... SLC31A1/CTR1 (Abcam, ab129067 WB/IF/FC). Rabbit polyclonal antibodies raised against VPS35 (Abcam 97545 ...
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bioRxiv - Synthetic Biology 2020Quote: ... and goat anti-rabbit IgG Fc (Abcam) were amine-coupled to CM3 chips and human and rabbit mAbs were captured to an average density of 320 ± 1.5 RU (s.e.m) ...
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bioRxiv - Microbiology 2021Quote: ... Protein binding between FXa-Fc or Fc and S protein was detected by immunoblotting using an anti-S protein antibody (ab272504, Abcam).
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bioRxiv - Immunology 2020Quote: ... and goat anti-Rabbit IgG Fc (Abcam, USA) was amine coupled to CM3 surface ...
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bioRxiv - Bioengineering 2023Quote: ... EPO receptor protein (Fc chimera active; Abcam, USA) was immobilized on Series S Sensor Chips CM5 (GE Healthcare ...
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bioRxiv - Developmental Biology 2023Quote: ... goat anti-rat IgG Fc 488 (ab97089 abcam), goat anti-mouse IgG Fc Alexa Fluor™ 680 (115625071 Jackson Immuno Research).
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bioRxiv - Developmental Biology 2023Quote: ... Sections were blocked in 10% FCS and then incubated overnight at 4°C with the following primary antibodies diluted in 2% FCS: LGALS1 (Abcam ab138513), RBP1 (Invitrogen PA528713) ...
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bioRxiv - Neuroscience 2021Quote: ... (Human Fibrinogen: ab208036 Abcam UK, Human Gelsolin: ITRP09965 Immunotag, USA, Human Apolipoprotein AIV: ab214567 Abcam UK ...
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bioRxiv - Immunology 2019Quote: ... INCA-6 (Abcam; ab145864), 11R-VIVIT (Thermo Fisher ...
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bioRxiv - Neuroscience 2022Quote: ... TMEM119 (106-6, Abcam) and anti-CD86 (GL-1 ...
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bioRxiv - Molecular Biology 2023Quote: ... IL-6 (Abcam ab259341), H3 (Abcam ab1791 ...
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bioRxiv - Molecular Biology 2023Quote: ... (IL-6, Abcam, ab222503), interlrukin-10 (IL-10 ...
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bioRxiv - Physiology 2024Quote: ... 6 (PSMC6) (Abcam, ab22639), Lysosomal membrane protein 1 (LAMP1 ...
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bioRxiv - Immunology 2024Quote: ... followed by the goat Anti-Rabbit IgG Fc AF568 (Abcam) secondary antibody ...