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• Non-canonical autophagy drives alternative ATG8 conjugation to phosphatidylserine

  Joanne Durgan, et al., bioRxiv - Cell Biology 2020

  Quote: ... Recombinant His-tagged human ATG4B (Abcam, ab188707) was pre-treated with 10 mM DTT for 15 mins ...
• Covalent inhibition of endoplasmic reticulum chaperone GRP78 disconnects the transduction of ER stress signals to inflammation and lipid accumulation in diet-induced obese mice

  Dan Luo, et al., bioRxiv - Pharmacology and Toxicology 2021

  Quote: Recombinant 6xHis-tagged human GRP78 protein (Abcam, Cambridge, UK) was biotinylated with EZ-Link Sulfo-NHS-Biotin (Thermo Fisher Scientific ...
• PARP Inhibition Impedes the Maturation of Nascent DNA Strands During DNA Replication

  Alina Vaitsiankova, et al., bioRxiv - Cell Biology 2021

  Quote: ... Rat recombinant anti-PCNA (Abcam, ab252848), Rat monoclonal anti-BrdU (Abcam ...
• TIRR regulates mRNA export and association with P bodies in response to DNA damage

  Michelle S Glossop, et al., bioRxiv - Molecular Biology 2024

  Quote: ... and incubated with 6µg recombinant GST-tagged XPO1 (ab131897, Abcam) for 1 hour at 4°C on a wheel ...
• The Primary Cilium and its Hedgehog Signaling in Nociceptors Contribute to Inflammatory and Neuropathic Pain

  Lindsey A. Fitzsimons, et al., bioRxiv - Neuroscience 2023

  Quote: ... Recombinant rat Sonic hedgehog (Shh) was purchased from Abcam. Stock solutions of PGE2 (1 μg/μl ...
• Temporal control of the integrated stress response by a stochastic molecular switch

  Philipp Klein, et al., bioRxiv - Cell Biology 2022

  Quote: ... recombinant GST-tagged PKR kinase domain (Abcam; 1/2/3/4.4/6.7/10/12.5 ng) or protein lysis buffer ...
• Functionality of the putative surface glycoproteins of the Wuhan spiny eel influenza virus

  Guha Asthagiri Arunkumar, et al., bioRxiv - Microbiology 2021

  Quote: ... recombinant hexahistidine-tagged HA was precomplexed with a mouse anti-his Alexa 647 antibody (Abcam) and goat - anti-mouse Alexa 647 antibodies ...
• Lysosomes mediate the mitochondrial UPR via mTORC1-dependent ATF4 phosphorylation

  Terytty Yang Li, et al., bioRxiv - Cell Biology 2022

  Quote: ... The assays were carried out in 50 μl mTOR reaction buffer with or without 200 μM ATP and/or the recombinant human His-tagged ATF4 (Cat. ab109946, Abcam) at 30°C for 45 min ...
• Precision RNAi using synthetic shRNAmir target sites

  Thomas Hoffmann, et al., bioRxiv - Genetics 2023

  Quote: ... according to manufacturer’s instructions using a recombinant rat monoclonal STAG1 antibody (Abcam ab241544, Lot:GR3334172-1; 1:200) after cooking 10 minutes at 110°C in antigen unmasking solution (Vector #H3301) ...
• Diversity-oriented synthesis encoded by deoxyoligonucleotides

  Liam Hudson, et al., bioRxiv - Biochemistry 2023

  Quote: ... His-GST-tagged USP7 (SinoBiological 11681-H20B) and His-tagged GST (Abcam ab89494) were immobilized to the streptavidin sensor chip (Cytiva BR100531 ...
• Heritable differences in synaptic zinc-transporter levels drive variation in learned birdsong

  David G. Mets, et al., bioRxiv - Neuroscience 2021

  Quote: ... The monoclonal anti-VGLUT2 antibody was raised in Mice immunized to a full length recombinant protein corresponding to rat VGLUT2 (Abcam, ab79157) and has been previously shown to stain VGLUT2 in zebra finches (86) ...
• Activation of SGK1.1 up-regulates the M-current in the presence of epilepsy mutations

  Elva Martin-Batista, et al., bioRxiv - Neuroscience 2021

  Quote: ... YFP-tagged SGK1.1 and GFP-tagged Kv7 subunits were detected using mouse anti-GFP monoclonal antibody (Abcam, ab290). Nedd4-2 was detected with a rabbit polyclonal antibody from Cell Signaling (4013S) ...
• An ELISA-based method for rapid genetic screens in Drosophila

  Taylor R Jay, et al., bioRxiv - Neuroscience 2021

  Quote: ... Recombinant GFP (abcam #ab84191) was diluted in 0.1%PBST ...
• α-Endosulfine regulates amyloid β 42 via the modulation of neprilysin activity

  Naoto Watamura, et al., bioRxiv - Neuroscience 2020

  Quote: ... recombinant ENSA (abcam ab92932), recombinant NSG-1 (Creative BioMart NSG1-332H) ...
• Dissection of a non-coding risk locus at 1p36.23 identifies ERRFI1 as a novel gene in the pathogenesis of psoriasis and psoriatic arthritis

  Oliver J. Gough, et al., bioRxiv - Genomics 2023

  Quote: ... Recombinant rabbit IgG (Abcam) and CoraLite 555-cojugated rabbit IgG control polyclonal antibody (Proteintech ...
• The dual function receptor kinase, OsWAKL21.2, is involved in elaboration of lipaseA/esterase induced immune responses in rice

  Kamal Kumar Malukani, et al., bioRxiv - Plant Biology 2019

  Quote: ... HRP tagged anti-Rabbit IgG secondary antibody (Abcam) (dilution 1:50000 ...
• BCAT1 inhibition affects CD8⁺ T cell activation, exhaustion, and tumoral immunity by altering iron homeostasis

  Francesca Lodi, et al., bioRxiv - Immunology 2023

  Quote: ... 0.5 μg of rhACO1 tagged with His (Abcam) was added to the reaction mixtures and the samples were rotated for 30 min at 37°C ...
• A screening platform based on epitope editing for drug discovery

  Biyue Zhu, et al., bioRxiv - Biochemistry 2019

  Quote: ... recombinant human PD-L1 protein and recombinant human tau protein were purchased from Abcam. HRP-conjugated goat anti-Mouse IgG (H+L ...
• USP50 suppresses alternative RecQ helicase use and deleterious DNA2 activity during replication

  Hannah L. Mackay, et al., bioRxiv - Cancer Biology 2024

  Quote: ... Rat αBrdU (Abcam) used at a concentration of 1:1000 and Mouse αBrdU (BD Biosciences ...
• Syndecan-3 enhances anabolic bone formation through WNT signalling

  Francesca Manuela Johnson de Sousa Brito, et al., bioRxiv - Cell Biology 2019

  Quote: ... recombinant mouse WNT3a protein (Abcam) 50ng/ml was added and cells cultured for 8 hours before lysis in Trizol.
• Human immunocompetent Organ-on-Chip platforms allow safety profiling of tumor-targeted T-cell bispecific antibodies

  S. Jordan Kerns, et al., bioRxiv - Cancer Biology 2021

  Quote: ... recombinant rabbit anti-CEA (Abcam) for samples without TCB treatment and monoclonal rat anti-CD45 (Invitrogen) ...
• Structural control of fibrin bioactivity by mechanical deformation

  Sachin Kumar, et al., bioRxiv - Bioengineering 2020

  Quote: ... Recombinant human αIIbβ3 integrin (Abcam) (1 mg/ml ...
• Immunoglobulin J chain as a non-invasive indicator of pregnancy in the cheetah (Acinonyx jubatus)

  Michael J. Byron, et al., bioRxiv - Physiology 2019

  Quote: ... Human recombinant IgJ (Abcam #140727) was used as a positive control at 16.67 μg/mL ...
• DDR1-INDUCED NEUTROPHIL EXTRACELLULAR TRAPS DRIVE PANCREATIC CANCER METASTASIS

  Jenying Deng, et al., bioRxiv - Cancer Biology 2020

  Quote: ... human recombinant CXCL5 (Abcam, ab9803), DNase I (1.5U) ...
• Advanced physiological maturation of iPSC-derived human cardiomyocytes using an algorithm-directed optimization of defined media components

  Neal I. Callaghan, et al., bioRxiv - Bioengineering 2022

  Quote: ... recombinant neuregulin 1β2 (ab73753; Abcam), metformin hydrochloride (ICN15169101 ...
• Validation of Tau Antibodies for Use in Western Blotting and Immunohistochemistry

  Michael J. Ellis, et al., bioRxiv - Neuroscience 2023

  Quote: ... Recombinant MAP2c protein (Abcam, #ab114686), carrying a ∼26 kDa Glutathione-S-Transferase (GST ...
• Repeat-based holocentromeres of the woodrush Luzula sylvatica reveal new insights into the evolutionary transition from mono- to holocentricity

  Yennifer Mata-Sucre, et al., bioRxiv - Genomics 2024

  Quote: ... Recombinant rabbit IgG (abcam, ab172730) and no-antibody inputs were used as controls ...
• STREAMLINED PROTEOME-WIDE IDENTIFICATION OF DRUG TARGETS INDICATES ORGAN-SPECIFIC ENGAGEMENT

  Tanveer Singh Batth, et al., bioRxiv - Biochemistry 2024

  Quote: ... Recombinant human pirin (Abcam, ab123170) was buffer exchanged to PBS and immobilized at 25 μg/ml to ~ 10,000 RUs on flow cell 2 (Fc2 ...
• Evidence that an HDAC2-targeted ASO persistently upregulates cortical acetylcholine and dopamine signaling via a CREB | Gs positive feedback loop

  Teresa H. Sanders, bioRxiv - Neuroscience 2020

  Quote: ... Secondary antibodies were goat anti rat and goat anti rat (Abcam). Membranes were imaged on the LiCor Odyssey fluorescence imaging system.
• Generation and validation of a CRF₁:Cre transgenic rat: The role of central amygdala CRF₁ in nociception and anxiety-like behavior

  Marcus M. Weera, et al., bioRxiv - Neuroscience 2021

  Quote: ... biotin tagged antibody (1:500; Abcam, catalog no. ab34771). Sections were then washed 3x with PBS and incubated in streptavidin-conjugated peroxidase (DAB-elite kit ...
• Topology-informed regulatory element collections coordinate cell identity gene expression programs

  Jie Lv, et al., bioRxiv - Molecular Biology 2024

  Quote: ... BRD4 was tagged using AF647 Anti-Brd4 antibody (Abcam).
• Evidence for Membrane Complex Assembly in Nanoelectrospray Generated Lipid Bilayers

  Matthias Wilm, bioRxiv - Synthetic Biology 2019

  Quote: ... Recombinant LLO from Abcam (Cambridge, UK) was an alternative source for the protein (residues 60 - 529 ...
• Neuroinflammation in the normal appearing white matter of multiple sclerosis brain causes abnormalities at the node of Ranvier

  Patricia Gallego Delgado, et al., bioRxiv - Neuroscience 2020

  Quote: ... LTα (recombinant human LT-α, Abcam) and IFNγ (recombinant rat IFNγ ...
• Assessment of CAR-T mediated and targeted cytotoxicity in 3D microfluidic TBNC co-culture models for combination therapy

  K. Paterson, et al., bioRxiv - Bioengineering 2021

  Quote: ... recombinant anti-EGFR antibody (ab32198, abcam), or PD-L1 ...
• Neuronal Proteostasis is mediated by the switch-like expression of Heme-regulated Kinase 1, acting as both a sensor and effector

  Beatriz Alvarez-Castelao, et al., bioRxiv - Neuroscience 2019

  Quote: ... or 500ng of recombinant eIF2a (abcam) were incubated with 2ug of purified 20S proteasome (Enzo ...
• Integrated Proteomics Identifies Neuritin (NRN1) as a Mediator of Cognitive Resilience to Alzheimer’s Disease

  Cheyenne Hurst, et al., bioRxiv - Neuroscience 2022

  Quote: ... Recombinant human Neuritin protein (Abcam, ab69755) was reconstituted in water to a concentration of 0.1 mg/mL ...
• DOCK3 regulates normal skeletal muscle regeneration and glucose metabolism

  Adrienne Samani, et al., bioRxiv - Genetics 2023

  Quote: Recombinant SORBS1 protein (Abcam; Cambridge, UK) was incubated with recombinant GST-DOCK3-PXXP or GST alone plasmids (constructs cloned into pGEX-6P-1 plasmid ...
• Spatial and temporal transcriptomics of SHH-medulloblastoma with chromothripsis identifies multiple genetic clones that resist to treatment and lead to relapse

  Ilia Kats, et al., bioRxiv - Cancer Biology 2023

  Quote: ... Recombinant anti-nestin antibody (abcam, #ab105389) raised in rabbit was used at a dilution of 1:100 and kept for overnight incubation ...
• Schwann cell release of p11 induces sensory neuron hyperactivity in Fabry disease

  Tyler B. Waltz, et al., bioRxiv - Neuroscience 2023

  Quote: ... Recombinant anti-SOX10 antibody (Abcam, EPR4007) in blocking solution was added for 1 hr (1:500) ...
• One for all – Human kidney Caki-1 cells are highly susceptible to infection with corona- and other respiratory viruses

  A. Daniels, et al., bioRxiv - Microbiology 2023

  Quote: ... Recombinant Anti-ACE2 antibody (Abcam, ab272500) was used at 1:500 before staining with Goat anti-Rabbit IgG (H+L ...
• STK25 is an IRF5 kinase that promotes TLR7/8-mediated inflammation

  Matthew R. Rice, et al., bioRxiv - Biochemistry 2023

  Quote: Recombinant human IRF5 protein (Abcam, ab173024) (500 ng ...
• A novel multi-functional role for the MCM8/9 helicase complex in maintaining fork integrity during replication stress

  Wezley C. Griffin, et al., bioRxiv - Cell Biology 2021

  Quote: ... and rat (Abcam, ab6325) (1:400 ...
• Zika virus spreads through infection of lymph node-resident macrophages

  Glennys V. Reynoso, et al., bioRxiv - Microbiology 2022

  Quote: ... ERTR7 (rat monoclonal, Abcam), B220 (clone RA3-6B2 ...
• Rab11A regulates the constitutive secretory pathway during Toxoplasma gondii invasion of host cells and parasite replication

  Venugopal Kannan, et al., bioRxiv - Microbiology 2019

  Quote: ... rat anti-cMyc (Abcam), mouse anti-SAG1 (our lab) ...
• Lmx1a is a master regulator of the cortical hem

  Igor Y. Iskusnykh, et al., bioRxiv - Neuroscience 2022

  Quote: ... anti-Ctip2 (rat, Abcam, catalog #ab18465 ...
• SRF transcriptionally regulates the oligodendrocyte cytoskeleton during CNS myelination

  Tal Iram, et al., bioRxiv - Neuroscience 2022

  Quote: ... Rat-anti-MBP (Abcam, ab7349 ...
• Clinically relevant concurrent BRAF and MEK inhibition alters differentiation states and sensitizes BRAF V600E-mutated high-grade gliomas to immune checkpoint blockade

  Jong-Whi Park, et al., bioRxiv - Cancer Biology 2023

  Quote: ... rat anti-MBP (Abcam), rat anti-CD8 (BioLegend) ...
• Uip4p modulates nuclear pore complex function in Saccharomyces cerevisiae

  Pallavi Deolal, et al., bioRxiv - Cell Biology 2021

  Quote: ... were detected by HRP tagged secondary anti-rabbit (Abcam ab97051) and anti-mouse (Santa Cruz sc-2005) ...
• Discovery of novel compounds as potent activators of Sirt3

  Célina Reverdy, et al., bioRxiv - Biochemistry 2022

  Quote: A N-terminal His tagged Sirt3(118-399) from Abcam was used for all the DEL experiments ...
• A Mycobacterium tuberculosis effector protein attacks host innate immunity by acting as an unusual ubiquitinating enzyme

  Jing Wang, et al., bioRxiv - Microbiology 2020

  Quote: In vitro ubiquitination assays of GST-tagged TRAF2 (ab204091, Abcam) and TAK1(ab132950 ...