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Citations for abcam :
3301 - 3350 of 10000+ citations for Recombinant Mouse Frizzled Homolog 1 Drosophila His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... Cells were washed with PBS and incubated with anti-mouse IgG H&L Alexa Fluor 594 secondary antibody (1:2000) (Abcam, ab150116) and DAPI (1:3000 ...
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bioRxiv - Microbiology 2023Quote: ... The 1 mg/mL antibody AB1 (mouse monoclonal 3519) in PBS was purchased from Abcam (aliquoted and stored at -20°C). The 2 mg/mL anti-mouse secondary antibody CF405S-AB2 (goat polyclonal IgG H+L ...
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bioRxiv - Cell Biology 2023Quote: ... and incubated in 50 μL of 2% goat serum in PBS containing mouse monoclonal anti-NR2A antibody (abcam #240884, 1:200) and rabbit monoclonal anti- Na+/K+-ATPase ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and mouse anti-phospho-histone H2AX (also known as γH2AFX; serine 139 -phosphorylated gamma histone H2A.X) (1:1000, Abcam, Cambridge, UK). After washing in PBS ...
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bioRxiv - Plant Biology 2023Quote: ... diluted to 1:125 was used as primary antibody and Donkey Anti-Mouse IgG H&L Alexa Fluor® 555(Abcam) diluted to 1:500 was used as secondary ...
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bioRxiv - Systems Biology 2023Quote: ... after which slides were incubated overnight in a humidity chamber with primary antibodies: UCP1 (rabbit anti-mouse, Abcam, ab155117, 1:400), PPAR-α (rabbit anti-mouse ...
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bioRxiv - Genetics 2023Quote: ... Coverslips were incubated overnight at 4°C in PBS+ with primary antibodies: mouse monoclonal anti-smooth muscle actin (SMA) (1:750, Abcam, ab7817) and rabbit polyclonal anti-SM22 (1:400 ...
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bioRxiv - Neuroscience 2023Quote: ... Blocking solution was replaced with a primary antibody solution (PBS 1X, 0.1% BSA, 0.1% Triton X-100) presenting a primary antibody against IBA1 (mouse monoclonal, abcam; ab178846; 1:500) at 4°C overnight ...
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bioRxiv - Cancer Biology 2024Quote: ... ready to use DISCOVERY OmniMap anti-Rb HRP (760-4311) for PHOX2B and Phospho-SMAD-1/5/9 or rabbit anti-mouse secondary (Abcam, ab133469) followed by DISCOVERY OmniMap anti-Rb HRP (760-4311 ...
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bioRxiv - Cell Biology 2024Quote: ... or Alexa Fluor-conjugated antibody (Alexa Fluor® 647) mouse monoclonal anti-histone H3 (phospho S10) antibody (1:250, Abcam ab196698)) ...
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bioRxiv - Neuroscience 2024Quote: ... Secondary antibodies used were goat anti-mouse IgG conjugated to Alexa Fluor 488/568 (1:1000; ab150113/ab175473, Abcam, Cambridge, UK) and goat anti-rabbit IgG conjugated to AlexaFluor 488/568 (1:1000 ...
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bioRxiv - Cell Biology 2024Quote: ... and then washed 3X with PBST and incubated for 1 hour in secondary antibody Goat Anti-Mouse IGG H&L Alexa Fluor® 488 (ab150113, Abcam, 1 ...
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bioRxiv - Molecular Biology 2024Quote: ... previously blotted membranes from Figure S2C,E were re-probed with 1:5000 mouse monoclonal anti-TATA binding protein antibody (Abcam #ab51841). The membrane in Figure S2H was probed for 1 h with 1:1000 rabbit anti-LARP7 antibody (Fortis/Bethyl #A303-723A ...
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bioRxiv - Microbiology 2024Quote: ... cells were washed 3X with 200 µL cold PBST before resuspension in 100 µL cold PBST containing a 1:200 dilution of goat α-mouse antibodies conjugated to AlexaFluor555 (Abcam). Cells were incubated for 1 h at 4 °C with end-over-end rotation before washing 3X with 200 µL cold PBST ...
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bioRxiv - Bioengineering 2024Quote: ... sections were washed three times with PBS and incubated with the secondary antibody (goat anti-mouse Alexa Fluor 488, 1:400, Abcam 150113) for 2 hr at room temperature ...
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bioRxiv - Bioengineering 2024Quote: ... the samples were incubated with the goat polyclonal secondary antibody to Mouse IgG - Alexa Fluor® 555 (Abcam, ab150114, 1/500) in 1 % BSA solution for 2 hours at room temperature in the dark ...
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bioRxiv - Molecular Biology 2019Quote: ... The concentration of Tf in mouse plasma was determined using mouse Tf ELISA kit (ab157724, Abcam, USA). Amount of Tf in plaque homogenates was also determined by western blot analysis using the anti-Tf antibody (1:2000 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... a pair of monoclonal antibodies (clone EPR16588, ab178846; clone EPR16589, ab178847) and a recombinant human protein (ab105593) are available from Abcam (Waltham, MA). Similarly ...
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bioRxiv - Genomics 2024Quote: ... we extracted the total protein from the mutant cells and conducted western immunoblotting using a recombinant anti-HA tag antibody (Abcam, Waltham, MA).
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bioRxiv - Cell Biology 2020Quote: ... Tissue sections were double labelled overnight at 4°C with antibodies against anillin (mouse or rabbit) and either CD34 (1:250, Abcam, Cambridge, UK), CD56 (1:200 ...
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bioRxiv - Cell Biology 2020Quote: ... Abberior® STAR orange 561 goat anti-mouse (Abberior GmbH) (1:500) and Alexa Fluor® 488 conjugated goat anti-guinea pig (Abcam, ab150185). The Abberior ® secondary antibodies were kindly provided by Abberior instruments.
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bioRxiv - Developmental Biology 2020Quote: ... brains were incubated with conjugated fluorescent secondary Alexa Fluor 555 donkey anti-mouse IgG (H+L) (Abcam; Material No. ab150106; 1:250) for 2 days at 4 °C.
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bioRxiv - Cell Biology 2022Quote: ... Cells were then incubated for 60 min at room temperature with primary antibody dilutions prepared in 3 % BSA in PBS as follows: mouse anti-Bub1 (ab54893, Abcam, 1:500); mouse anti-Mad2 (sc-65492 ...
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bioRxiv - Neuroscience 2021Quote: ... membrane was probed with the following primary antibodies for overnight at 4°C: Rabbit anti-mouse LINE-1Orf1p (Abcam, ab216324, 1:1000), Mouse anti-GAPDH antibody (HRP conjugated ...
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bioRxiv - Cell Biology 2019Quote: ... or rabbit anti-TgTOM40 (1:2000, (van Dooren et al., 2016)) antibodies coupled to secondary horseradish peroxidase (HRP)(Promega for mouse and rabbit, Abcam for rat) conjugated antibodies (1:10,000 ...
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bioRxiv - Cell Biology 2019Quote: ... membranes were incubated over night at 4°C with mouse anti-phosho-PP1 antibody (Wu et al., 2009) (pospho-T320 Abcam, 1:1000) After washing ...
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bioRxiv - Neuroscience 2021Quote: ... Horseradish peroxidase-labeled conformation-sensitive secondary anti-mouse IgG for IP or anti-rabbit VeriBlot for IP Detection antibody (both at 1:20000; Abcam, Cambridge, MA) was detected by SuperSignal West Femto Maximum Sensitivity substrate (Thermo Fisher Scientific).
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bioRxiv - Neuroscience 2020Quote: ... for 1 hr at room temperature and then incubated with the following primary antibodies (diluted in 5% non-fat milk in 0.1% PBS-Tween 20) for 18 hrs at 4°C: Mouse monoclonal Total OXPHOS rodent WB cocktail (Abcam ab110413, 1:1,000); mouse monoclonal Total OXPHOS blue-native WB cocktail (Abcam ab110412 ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were washed and incubated under darkness at RT with a goat anti-mouse secondary antibody (1:500, Alexa fluor 488, Abcam, Cambridge, UK) in 0.1% Tx-100 ...
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bioRxiv - Immunology 2020Quote: ... The ELISA plates were washed 4 times by Blocking Buffer and ALP-conjugated Goat Anti-Mouse IgG Fc (Abcam, ab98710, 1:1000) in 50 μL Blocking Buffer was added ...
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bioRxiv - Neuroscience 2020Quote: ... and incubated overnight at 4° C with the primary monoclonal mouse antibody MOAB-2 against beta amyloid (1:500; Abcam Cat# ab126649) (Youmans et al. ...
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bioRxiv - Neuroscience 2020Quote: ... every fourth section throughout the hippocampus/dentate gyrus was collected and stained with panel 3 = anti-mouse BrdU (ab8152, Abcam, 1:500), anti-mouse doublecortin (DCX ...
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bioRxiv - Pathology 2021Quote: ... KIM-1 was also measured in urine as a marker for PT injury using a mouse ELISA kit (Abcam, Cambridge, MA; ab119596). As a marker of distal nephron injury ...
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bioRxiv - Immunology 2022Quote: ... The plates were washed three times with PBST and incubated with horseradish peroxidase-conjugated goat anti-mouse IgG (Abcam, ab6789, 1:10,000), goat anti-mouse IgG1 (Abcam ...
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bioRxiv - Biophysics 2022Quote: ... cells were fixed in ice-cold methanol at −20 before washing three times in PBS containing 10% horse serum (HS) for 5min each and being incubated with a mouse monoclonal primary antibody against α-tubulin (DMA1, 1:1000 dilution, Abcam, UK) for 1 hour at RT ...
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bioRxiv - Microbiology 2020Quote: ... 1:300 dilution of mouse anti-PfDHODH #3 (a kind gift from Margeret Phillips, University of Texas Southwestern) or 1/5000 mouse anti-PfAldolase-HRP (Abcam, Cat #ab38905). The next day ...
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bioRxiv - Neuroscience 2021Quote: ... diluted in PBS plus 2% NBCS and 0.3% Triton X-100: mouse anti-Glutamate decarboxylase (65 kD isoform, GAD65; 1:1500; ab26113; Abcam, Cambridge, United Kingdom), rabbit anti-Calbindin (1:3000 ...
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bioRxiv - Genomics 2022Quote: ... Cells were collected and incubated with antibodies against Anti-Mouse Integrin alpha 6 antibody (GoH3) conjugated to PE (1:600) (Abcam, Cat#: ab95703), a 1:200 CD104 antibody conjugated to FITC (Biolegend ...
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bioRxiv - Microbiology 2020Quote: ... for 2 h at room temperature and a secondary antibody (1:500; Donkey anti-mouse IgG H&L, Alexa Fluor® 568, Abcam #ab175472) for 1 h at room temperature in the dark ...
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bioRxiv - Bioengineering 2021Quote: ... the channels were incubated at 4 °C for 16 h with the following primary antibodies: anti-mouse laminin MEC5 (rat IgG; Catalog number ab2466; 1:50; Abcam, Cambridge, UK), anti-mouse collagen IV (rabbit IgG ...
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bioRxiv - Neuroscience 2019Quote: ... dried skimmed milk in Tris-buffered saline containing 0.1% Tween-20 (TBS-T) and incubated overnight at 4°C with primary antibodies against NDUFS4 (Abcam, mouse, 1:500), NSE (Dako ...
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bioRxiv - Pathology 2020Quote: Formalin-fixed paraffin-embedded tissue sections were subjected to antigen retrieval with EDTA buffer in high pressure heated water bath and staining was performed using either mouse monoclonal ERRα (1:2500, Abcam, Cambridge, MA) or ERRγ (1:400 ...
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bioRxiv - Neuroscience 2021Quote: ... membranes were incubated for 1h with their respective secondary fluorescent antibodies: anti-mouse (1:2,500, IRDye 800, Abcam, Cat# ab216772, RRID: AB_2857338) and anti-rabbit (1:2,500 ...
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bioRxiv - Pathology 2021Quote: ... The RBD-specific IgA titer of BAL samples was next evaluated by ELISA as described above with Goat anti-mouse IgA alpha chain-HRP (Abcam, 1:3000).
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bioRxiv - Genetics 2021Quote: ... embryos were washed 3 times 10 minutes in 1x PBS with 0.1% Triton-X100 and 0.1% BSA and incubated overnight at 4°C with the secondary antibody goat-anti-mouse A568 1:500 (Abcam, antibody ID# ab175473). On the 4th day ...
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bioRxiv - Immunology 2021Quote: ... Concentration of circulating LPS-binding protein (LBP) was determined in plasma samples (diluted 1:5) using Mouse LBP ELISA Kit (Abcam, Cambridge, MA) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... The pellet was re-suspended with 0.9% of sodium chloride solution and reacted with a rabbit anti-mouse HMGB1 primary antibody for 3 hrs at room temperature (1:350, abcam, Cat. #ab18256), followed by a goat anti-rabbit secondary antibody conjugated with Cy3 for 1hr at room temperature (1:500 ...
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bioRxiv - Microbiology 2021Quote: ... was used for indirect immunofluorescence microscopy at a dilution of 1:300 and mouse monoclonal antibody against nuclear pore complex proteins (Abcam, Cambridge, MA) was used for indirect immunofluorescence microscopy at a dilution of 1:500 ...
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bioRxiv - Cell Biology 2021Quote: ... lungs were incubated with conjugated fluorescent secondary Alexa Fluor 555 donkey anti-mouse IgG (H+L) (Abcam; Material No. ab150106; 1:250) for two days at 4 °C.
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bioRxiv - Developmental Biology 2022Quote: ... for 1 h at RT and directly labelled overnight at 4°C with primary antibodies (1:400 mouse mAB for sarcomeric alpha-actinin [CloneEA-53, Sigma-Aldrich, A7811] and 1:200 rabbit pAB for cardiac Troponin T [Abcam, ab45932]). After washing cells once with PBS ...