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Citations for abcam :
3251 - 3300 of 10000+ citations for Recombinant Mouse Frizzled Homolog 1 Drosophila His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... Primary antibodies were as follows: mCherry (used for mRuby, mCherry and TdTomato, made in mouse, monoclonal, ABCAM AB167477, concentration 1:1000), ChAT (choline acetyltransferase ...
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bioRxiv - Bioengineering 2020Quote: ... Sections were blocked with PBS-BSA 5% w/v for 30 min at RT and incubated with the primary antibodies (collagen type II, mouse monoclonal antibody, DSHB, II-II6B3, 1:100; collagen type I, rabbit monoclonal antibody, Abcam, ab138492) overnight at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... Tissue was then incubated for 2 hours at room temperature in a PBTGS secondary antibody solution including goat anti-mouse Alexa568 (1:200; Abcam [ab175473]), goat anti-rabbit Cy2 (1:200 ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were washed and incubated in darkness with goat anti-mouse secondary antibodies (1:500, Alexa fluor 488, Abcam, Cambridge, UK) in 0.1% Tx-100 ...
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bioRxiv - Molecular Biology 2021Quote: ... cells were permeabilized using 0.1% Triton-X and incubated for 2 hours at room temperature with mouse monoclonal antibody against sarcomeric α-actinin (1:100; Abcam), followed by an incubation of 1 hour with the secondary antibody conjugated to Alexa Fluor-488 (Life Technologies) ...
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bioRxiv - Immunology 2021Quote: ... Fixed cells were blocked with 5% BSA in PBS for 1 hr at room temperature and incubated with rabbit anti-mouse Ki67 primary antibody (Abcam ab15580) at 1:200 dilution overnight at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... and incubated at RT for 2 h with mouse anti-CTLA-4 monoclonal antibody (diluted: 1:3 000; Abcam, Cambridge, UK) as the primary antibody ...
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bioRxiv - Biophysics 2020Quote: ... the samples were incubated with the appropriate secondary antibody (polyclonal goat anti-mouse IgG H&L, 1:1000, Abcam, Cambridge, UK) diluted in blocking solution for 45 min at room temperature ...
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bioRxiv - Biophysics 2022Quote: ... Membranes were washed 3 times for 10 minutes at room temperature with TBST and incubated with either anti-Mouse IgG-HRP (1:20 000, Abcam, ab6728) or anti-Rabbit IgG-HRP (1:20 000 ...
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bioRxiv - Bioengineering 2022Quote: ... samples were washed x3 times with PBS and incubated for 2 h at room temperature with corresponding secondary antibody (α-mouse. 488, 1:500, ab150077, abcam; α-rabbit ...
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bioRxiv - Bioengineering 2022Quote: ... samples were washed x3 times with PBS and incubated for 2 h at room temperature with corresponding secondary antibody (α-mouse. 488, 1:500, ab150077, abcam; α-rabbit. 488,1:500, ab150077, abcam; α-goat ...
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bioRxiv - Microbiology 2022Quote: ... Slides were washed once again and HRP-conjugated secondary antibody was added at a 1:5000 concentration (Goat anti-mouse: ThermoFisher, Cat #A21426; Goat anti-rabbit: Abcam, Ab6721). Slides were washed twice prior to application of DAB developing reagent (Vector Laboratories ...
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bioRxiv - Microbiology 2021Quote: ... the samples were incubated with goat anti-mouse IgG Alexa Fluor 488 secondary antibody (1:1000 dilution; Abcam, Cambridge, MA, USA) for 1 h at 25℃ and the slides were mounted in antifade Mounting Medium with DAPI (Vectashield ...
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bioRxiv - Cancer Biology 2022Quote: ... B16F10 cells were stained with a 1:10 dilution of individual animal serum followed by detection with a 1:1000 dilution of a goat anti-mouse Fc FITC secondary (Abcam ab98716) and fixation with 4% formalin ...
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bioRxiv - Cell Biology 2022Quote: ... and incubated with the indicated primary antibodies diluted in blocking buffer at 4°C overnight: mouse-pH3 (1:2000, Abcam, ab14955), rabbit-Ki67 (1:500 ...
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bioRxiv - Neuroscience 2022Quote: ... sections were incubated at 4°C overnight in a solution of mouse anti-c-fos primary antibody (1:1000 dilution, Abcam, UK) or mouse anti-TPH2 primary antibody (1:100 dilution ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were then washed in PBS (3×10 min) and incubated for 2h in secondary antibody diluted in blocking solution (1:1000 Goat-α-mouse AlexaFluor488, abcam ab150113 ...
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bioRxiv - Bioengineering 2019Quote: ... followed by overnight staining with 1:300 dilutions of rat-anti-C-peptide (DSHB; GN-ID4-S) and mouse-anti-glucagon (ABCAM; ab82270) primary antibodies ...
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bioRxiv - Systems Biology 2020Quote: ... cells were stained at 4°C o/n in 50 µL of the 1st antibody diluent (rabbit anti-ZO-1, 61-7300, Thermo Fisher Scientific Inc., MA, USA; anti-MITF, mouse anti-MiTF, ab80651, Abcam plc. ...
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bioRxiv - Immunology 2019Quote: ELISAs were performed using Mouse MIP-1 alpha (CCL3) ELISA Ready-SET-Go ELISA kit (eBi-oscience™) and Prostaglandin E2 ELISA Kit (Abcam) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... the blot was incubated with 1:1000 diluted buffer of secondary rabbit anti-mouse HRP conjugated antibody (Cat # 6728, Abcam, UK) in 1X PBS for 1 h at room temperature ...
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bioRxiv - Molecular Biology 2020Quote: ... proteins were blocked with 1% normal goat serum (30 min at room temperature) followed by a goat anti-mouse IgG2a antibody (Abcam, ab98694). After additional blocking with normal goat serum ...
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bioRxiv - Immunology 2021Quote: ... For detection of IgG subclasses all serum samples were diluted to 1 total IgG EU and incubated at 37 °C for 1 h prior to detection with Alkaline Phosphatase conjugated anti-mouse IgG subclass-specific secondary antibodies (Southern Biotech or Abcam) incubated for 1 h at 37 °C ...
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bioRxiv - Evolutionary Biology 2020Quote: ... western blotting (WB) and WB blocking conditions were as follows: mouse anti-β-actin (Abcam, 8226, WB 1:2000 in BSA), mouse anti-ZEB2 (Origene ...
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bioRxiv - Cell Biology 2020Quote: ... Human and mouse lung sections were stained for colocalization with primary and secondary antibodies: ANT1 (Abcam #ab102032 rabbit polyclonal, 1:1000), pan ANT (anti-ANT1/2/3 Abcam ...
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bioRxiv - Biochemistry 2021Quote: ... Membranes were then washed 5x with TBST and probed with 1/5000 FITC-labelled goat anti-mouse IgG secondary pAb (Abcam ab6785) and imaged for fluorescence on the Typhoon FLA7000 biomolecular imager (GE healthcare).
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bioRxiv - Cell Biology 2021Quote: ... with secondary antibody goat anti-mouse IgM mu chain (dilution 1:2000) conjugated with Alexa Fluor 488 (ab150121, Abcam, Cambridge, UK). Only one primary antibody was used in each experiment.
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bioRxiv - Bioengineering 2021Quote: ... Primary antibody solutions were prepared by diluting one or more of the following antibodies in blocking buffer: mouse anti-HNF4a (1/200 from stock, Abcam ab41898), goat anti-OPN (1/60 from stock ...
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bioRxiv - Bioengineering 2021Quote: ... Secondary antibody solutions were prepared by diluting one or more of the following secondary antibodies in blocking buffer: DyLight 550-conjugated donkey anti-mouse IgG (1/50 from stock, Abcam, ab98767) and DyLight 488-conjugated donkey anti-goat IgG (1/50 from stock ...
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bioRxiv - Molecular Biology 2021Quote: ... followed by overnight incubation at 4 °C in 1% BSA with primary antibodies: mouse monoclonal [EA-53] to sarcomeric alpha-actinin (Abcam ab9465), rabbit polyclonal anti-phospho-Histone H3 (Ser10 ...
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bioRxiv - Immunology 2020Quote: ... all serum samples were diluted to 1 total IgG ELISA unit and then detected with anti-mouse IgG subclass-specific secondary antibodies (Southern Biotech or Abcam). The results of the IgG subclass ELISA are presented using OD values instead of the ELISA units used for the total IgG ELISA ...
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bioRxiv - Microbiology 2020Quote: ... 3 colonic and 1 intestinal tissue fragments of each mouse were incubated with a primary polyclonal 1:200 rat anti-E-cadherin (#ab11512; Abcam USA) in PBS–3% BSA for overnight at 4 °C ...
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bioRxiv - Cancer Biology 2022Quote: ... Mouse monoclonal anti-human-ATM(phospho-S1981) which stains the homologous mouse ph-ATM(phosphor-S1987) was used at 1:500 (Abcam ab36810). The slides were incubated in biotin-conjugated secondary antibodies ...
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bioRxiv - Microbiology 2022Quote: ... for 2 h at RT followed by two washed with PBS and 1:200 secondary antibody donkey anti–mouse-Alexa Fluor 568 (ab175700, Abcam, USA). To detect the total E-cadherin ...
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bioRxiv - Cell Biology 2022Quote: In a separate plate the MYH Alpha-LISA was performed using 1:2,000 dilution of mouse monoclonal BA-G5 antibody to α-MYH (abcam, ab50967), 1:2,000 dilution of rabbit polyclonal β-MYH (abcam ...
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bioRxiv - Neuroscience 2022Quote: ... Antibodies raised against α-syn C-terminus (Mouse anti α-syn, Amino Acid (AA) 124-134 (1:1,000) and ab1903 (Abcam); Mouse anti α-syn 4B12 ...
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bioRxiv - Neuroscience 2023Quote: ... the sections were incubated with secondary antibodies Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) (1:700; Abcam, ab150115) and Goat Anti-Rabbit IgG Fc (Alexa Fluor® 488 ...
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bioRxiv - Neuroscience 2022Quote: ELISA kits were used to quantitate the mouse plasma levels of YKL-40 (1:40 dilution, #AB238262; ABCAM, Cambridge, MA, USA), IL-6 (1:4-1:2 dilution ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were washed three times in PBS for 15 min and incubated in secondary antibody for 2 h at room temperature: anti-mouse IgG (1/1,000, HRP-conjugated, Abcam, No. ab205719), anti-guinea pig IgG (H + L ...
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bioRxiv - Cell Biology 2024Quote: ... Paraffin embedded liver sections (3 μm) from human and mouse liver samples were stained for aSMA (1:100, Abcam, Cat#ab5694), KI67 (1:50 ...
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bioRxiv - Neuroscience 2024Quote: ... and incubated for 2 hours at room temperature with horseradish peroxidase-conjugated anti-mouse IgG secondary antibody (dilution 1:5000, Abcam #ab6789). After washing again ...
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bioRxiv - Immunology 2024Quote: ... After incubating for one hour in a 1:100,000 dilution of HRP-conjugated anti-IgG secondary (goat anti-mouse IgG (Abcam; RRID: AB_955439) for mouse serum or mouse-anti-monkey IgG (SouthernBiotech ...
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bioRxiv - Genetics 2024Quote: ... the samples were incubated in rabbit anti-DPY-27 antibody (purified antibody) [50] at 1:200 dilution and mouse anti-nuclear pore complex [Mab414] antibody (Abcam ab24609) at 1:1000 dilution ...
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bioRxiv - Cell Biology 2024Quote: ... Secondary antibodies and dilutions used for stains of cultured cells: Donkey anti-mouse (Alexa Fluor® 488) 1:500 (Abcam, #ab150105). Cells were counter-stained with DAPI 1:10,000 (Millipore Sigma ...
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bioRxiv - Molecular Biology 2023Quote: ... The prepared coverslips were incubated with the following primary antibodies prepared in mouse monoclonal anti-TFAM antibody (Abcam, #ab119684, 1:500) or monoclonal anti-TOM20 antibody (Cell Signaling Technology ...
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bioRxiv - Neuroscience 2023Quote: ... For double labeling, GFAP antibody was incubated with 8-hydroxyguanosine (8OHG, marker of RNA oxidation) (1:10000, mouse/IgG, Abcam, ab62623), AT100 (1:500 ...
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bioRxiv - Cell Biology 2023Quote: ... mouse monoclonal anti-KAHRP clone 18.2 (European Malaria Reagent Repository, United Kingdom) diluted 1:500 and mouse monoclonal anti-GAPDH (Abcam, United Kingdom) diluted 1:5000 ...
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bioRxiv - Cancer Biology 2023Quote: ... Myotubes were washed with PBS and incubated with anti-mouse IgG H&L Alexa Fluor 594 secondary antibody (1:2000) (Abcam, ab150116) and DAPI (1:3000 ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were washed with PBS and incubated with anti-mouse IgG H&L Alexa Fluor 594 secondary antibody (1:2000) (Abcam, ab150116) and DAPI (1:3000 ...
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bioRxiv - Microbiology 2023Quote: ... The 1 mg/mL antibody AB1 (mouse monoclonal 3519) in PBS was purchased from Abcam (aliquoted and stored at -20°C). The 2 mg/mL anti-mouse secondary antibody CF405S-AB2 (goat polyclonal IgG H+L ...