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Citations for abcam :
2101 - 2149 of 2149 citations for Recombinant Human PSG1 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... Cells were then stained for intracellular spike protein using an Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody (Abcam, ab275759, 1:50) followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor 488 ...
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bioRxiv - Immunology 2021Quote: ... Concentration of circulating LPS-binding protein (LBP) was determined in plasma samples (diluted 1:5) using Mouse LBP ELISA Kit (Abcam, Cambridge, MA) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... was used for indirect immunofluorescence microscopy at a dilution of 1:300 and mouse monoclonal antibody against nuclear pore complex proteins (Abcam, Cambridge, MA) was used for indirect immunofluorescence microscopy at a dilution of 1:500 ...
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bioRxiv - Cell Biology 2022Quote: ... Electroporated cells were seeded in StemMACS iPS-Brew XF with Rho-associated protein kinase (ROCK) inhibitor Y-27632 (10 μM, Abcam, Cambridge, UK) and 1x CloneR (Stemcell Technologies ...
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bioRxiv - Immunology 2022Quote: ... or CD8+ cells using a rabbit-raised anti-SARS-CoV-2 S protein antibody (1:5000 dilution, ab272504, Abcam plc, Cambridge UK), a rabbit monoclonal anti-CD4 antibody (1:250 dilution ...
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bioRxiv - Microbiology 2022Quote: ... paraffin-embedded sections were firstly subjected to immunostaining with a commercially available and diluted (1:5,000) rabbit-anti-SARS-CoV-2 spike (S) protein (ab272504, Abcam Plc., Cambridge, UK), followed by staining with a peroxidase-conjugated secondary antibody ...
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bioRxiv - Neuroscience 2022Quote: ... the supernatant calibrated into the same protein concentration was used for the measurement of oxytocin levels using an ELISA kit (Abcam, lot. 133050), according to the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2022Quote: Nuclear protein isolations from frozen muscle tissue were performed using a commercial kit (Nuclear Extraction Kit, catalog# ab113474; Abcam; Waltham, MA, USA). Briefly ...
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bioRxiv - Neuroscience 2024Quote: ... 30mg of protein from scraped cells at day N1 and N14 were analyzed with the Cathepsin D Activity assay kit (ab65302; Abcam, Waltham, MA) following the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2023Quote: ... They were then washed with PBS and incubated with 1° antibodies (α-NDUFV2, 15301-1-AP, Protein tech, α-tubulin, Abcam, ab179513) in 5% milk 0.1% Tween 20 ...
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bioRxiv - Bioengineering 2023Quote: ... Equal amounts of protein of EV or lysate samples were then subjected to western blot analysis for ALIX (ab186429; Abcam, Cambridge, UK) at 1:1000 ...
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bioRxiv - Cancer Biology 2023Quote: ... end-stage bulk tumor and 4- to 15-weeks control HDTVi liver protein lysates (50 μg) were analyzed using Fluorometric Caspase-1 Assay Kit (Abcam, cat.no. ab273268) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... Detection of thiophosphate ester tagged proteins from the in vitro kinase assay was done with a specific anti-thiophosphate ester antibody (#ab92570 Abcam 1:10000). Antibody against total CDC-42 was use for the loading control (#sc-390210 ...
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bioRxiv - Systems Biology 2024Quote: ... was conducted to normalize protein concentrations between all samples and hexokinase activity was measured according to the manufacturer’s protocol (Abcam, Cambridge, MA; ab136957), using 10 μg of protein per condition ...
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bioRxiv - Microbiology 2024Quote: ... and a rabbit antibody recognizing cellular proteins or epitope tags (anti-EEA1, Cell Signaling Technology, 2411, 1:75; anti-TGN46, Abcam, ab50595, 1:600; anti-GM130, Abcam, ab52649 ...
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bioRxiv - Cell Biology 2020Quote: ... Co-immunoprecipitation of α-tubulin interacting proteins was performed from total cell lysates using α-Tubulin specific antibodies (rabbit anti-α-tubulin, ab52899, Abcam) and Protein A/G Sepharose beads (Santa Cruz Biotechnology) ...
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bioRxiv - Cell Biology 2020Quote: ... The eluates were boiled with LDS protein loading buffer (Thermo, NP0008) and loaded on NuPAGE for gel electrophoresis and Western blotting with antibody to ANT1 (Abcam, ab102032, 1:3000). In some experiments ...
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bioRxiv - Genetics 2021Quote: ... ran side by side in a Criterion Precast Gradient 4-15% SDS-PAGE gel for western blotting probing of key intracellular organellar markers and proteins of interest: HSP60 (Abcam, cat. number ab46798), Calnexin (Abcam ...
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bioRxiv - Cell Biology 2019Quote: ... with 2-8 μg total protein loaded per lane and the following dilutions of mouse mAbs: anti-K14 (1:500; clone LL002, Abcam Inc., Cambridge, UK), anti-K42 (1:500) ...
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bioRxiv - Neuroscience 2020Quote: ... Proteins were transferred to 0.2 um nitrocellulose or PVDF membranes and detected with primary antibodies targeting LRRK2 (ab133474, Abcam, RRID: AB_2713963, 1:500), pS935 LRRK2 (ab133450 ...
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bioRxiv - Plant Biology 2021Quote: ... For each ChIP/re-ChIP assay, 20 μg DNA/protein complexes were immunoprecipitated with anti-H3K27me3 (Milipore, Cat No 07-449) and anti-H3K18ac (Abcam, Cat No ab1191) antibodies using the Re-ChIP-IT kit (Active Motif ...
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bioRxiv - Neuroscience 2020Quote: ... Equal amounts of protein were then processed through an ELISA based kit for detecting p65 (NF-κB p65 Transcription Factor Assay Kit, abcam, Cambridge, MA, #ab133112). Absorbance at 450 nm was read with SpectraMax iD3 plate reader (Molecular Devices ...
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bioRxiv - Neuroscience 2020Quote: ... sections were preincubated (1-hour) at RT in a protein blocking solution (Expose Rabbit-Specific HRP/DAB detection IHC Kit, Abcam, Cambridge, UK, ab80437). Then ...
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bioRxiv - Immunology 2021Quote: ... SARS-CoV-2 infected cells were visualized using SARS-CoV-2 S protein specific immunochemistry staining with anti-SARS-CoV-2 spike glycoprotein S1 antibody (Abcam, Cambridge, Great Britain) as described previously [64] ...
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bioRxiv - Neuroscience 2021Quote: ... Co-labeling for astrocytes was performed using the same anti-glial fibrillary acidic protein antibody described above (GFAP; Abcam, ab53554; 1:250 dilution). Secondary antibody incubation labeling GFAP positive cells was performed for 1 hour at room temperature using Alexa Fluor 488-conjugated Donkey anti-Goat (ThermoFisher ...
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bioRxiv - Physiology 2021Quote: ... A commercially available monoclonal antibody was used to detect electron transport chain proteins (rodent OXPHOS Cocktail, ab110413; Abcam, Cambridge, UK, 1:250 dilution), including V-ATP5A (55kDa) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Membranes were then probed to determine protein levels using the following antibodies diluted in 5% non-fat milk in 1x TBST: matrix metalloproteinase 9 (MMP9) (Abcam ab38898, 1:1000) and MMP2 (Abcam ab92536 ...
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bioRxiv - Genetics 2019Quote: ... Proteins were transferred to a polyvinylidene difluoride membrane and probed with well-characterized monoclonal antibody of anti-Fibrillin 1 (Abcam ab124334, Cambridge, UK).
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bioRxiv - Bioengineering 2019Quote: ... Tangential cortical sections of 30 μm-thickness were sliced using a cryostat and directly mounted on gelatin-subbed glass slides for immunohistological staining of GFAP (glial fibrillary acidic protein, Abcam, Cambridge, MA, USA) for astrocytes ...
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bioRxiv - Microbiology 2020Quote: Paraffin embedded tissue sections were stained for SARS-CoV-2 nucleocapsid protein (1:500) and ionized calcium binding adaptor molecule 1 (Abcam; ab5076; 1:50) using a Leica Bond RXm automated staining instrument following permeabilization using 0.01% Triton X diluted in Trisbuffered saline (TBS) ...
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bioRxiv - Physiology 2020Quote: ... Nuclear sirtuin 1 content and activity was assessed using 10 µg (5 μL) of isolated nuclear protein fractions through a fluorometric assay (Sirt1 Activity Assay Kit, #ab156065, Abcam, Whitby, Ontario, Canada). Another set of samples weighing 5-10 mg were homogenized in RIPA buffer (150 mM sodium chloride ...
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bioRxiv - Microbiology 2020Quote: ... the fixed cells were labeled with primary antibodies including goat anti-major outer-membrane protein (MOMP; Meridian, Memphis, TN) and mouse or rabbit anti-six histidine tag (Genscript, Nanjing, China and Abcam, Cambridge, UK, respectively). For experiments using CRISPR interference ...
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bioRxiv - Plant Biology 2024Quote: ... Protein expression of rSPL9-GFP was verified by SDS-PAGE and immunoblotting using an anti-GFP antibody (Abcam, Cat# ab290, 1:10,000 dilution) and HRP-conjugated secondary antibody (Promega ...
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bioRxiv - Physiology 2024Quote: ... A commercially available monoclonal antibody was used to detect electron transport chain proteins (rodent OXPHOS Cocktail, ab110413; Abcam, Cambridge, UK, 1:250 dilution), including V-ATP5A (55kDa) ...
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Mucociliary Clearance Augmenting Drugs Block SARS-Cov-2 Replication in Human Airway Epithelial CellsbioRxiv - Microbiology 2023Quote: ... while sections of caspase and protein S staining were incubated with goat anti-rabbit IgG secondary antibody conjugated with HRP (Abcam ab6721, 1:1,000). ImmPACT DAB Peroxidase (HRP ...
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bioRxiv - Developmental Biology 2023Quote: Expression of Br-Z1 and Blimp-1 proteins was inferred from staining knock-in strains with chicken anti-GFP (Abcam Ab13970 1:500) and an anti-chicken secondary antibody (Invitrogen A-21437 1:1000).
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bioRxiv - Microbiology 2023Quote: ... Primary antibody incubation was conducted for 3 hours at room temperature (1:2,000 α-N protein, Sino Biological 40143-R001; 1:500 α-nsp3, abcam ab283958) followed by a 1 hour ...
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bioRxiv - Pathology 2024Quote: ... IHC protocol modified from a previously described protocol(37, 38) using a rabbit anti-SARS-CoV-2 S protein antibody (Abcam, ab272504, Cambridge, UK) at 1:5000 dilution (0.2 µg/mL) ...
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bioRxiv - Cell Biology 2024Quote: ... Transfer quality and total protein were assessed by Ponceau-S staining before the membranes were probed for Myc-tag (Abcam, #ab9106, 1:1000). Fluorophore-conjugated secondary antibodies were used for detection using the Licor Odyssey CLx system (anti-rabbit IgG ...
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bioRxiv - Neuroscience 2021Quote: ... the cells were incubated with the primary antibodies of interested proteins at 4°C overnight (Nestin, 1:1000, Sigma; Tuj1, 1:1000, Sigma; GFAP, 1:1000, Abcam; Ki67, 1:1000, ProteinTech). The next day ...
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bioRxiv - Neuroscience 2021Quote: ... Primary antibody incubations were performed for 24 hours at 4°C using an anti-glial fibrillary acidic protein (GFAP; Abcam, ab53554; 1:250 dilution) antibody ...
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bioRxiv - Cell Biology 2021Quote: ... Rat antibodies against the following proteins were used: α18 (gift from Professor A. Nagafuchi, Nara Medical University, Japan; 1:50) and rat E-Cadherin (Abcam 13-1800; 1:500). Goat antibody against p114 RhoGEF was purchased from Sapphire Bioscience (EB06163 ...
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bioRxiv - Genetics 2022Quote: ... for 1 hour at 4°C and then incubated overnight at 4°C with 1:1 mix of protein A/G Dynabead magnetic beads conjugated to H3K9me3 antibody (Abcam, cat# 8898 RRID: AB_306848).
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bioRxiv - Biochemistry 2023Quote: Secreted and intracellular HiBiT-tagged FV or AAT proteins as well as expressed ERGIC-53 were also detected by Western blotting with the Nano-Glo® HiBiT Blotting System (LgBiT protein) and anti-Factor V (Abcam, ab108614, 1:3300) or anti-LMAN1 (abcam ...
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bioRxiv - Neuroscience 2022Quote: ... the slices were incubated in the solution of primary antibodies: anti-glial fibrillar acidic protein (GFAP) rabbit polyclonal antibody (Abcam, Cambridge, UK, catalog number ab7260) and anti-neuronal nuclear protein (NeuN ...
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bioRxiv - Microbiology 2023Quote: ... rabbit antibodies recognizing cellular proteins (anti-TGN46, Abcam, ab50595, 1:600; anti-EEA1, Cell Signaling Technology, 2411, 1:75; anti-VPS35, Abcam, ab157220, 1:200). PLA was performed with Duolink reagents (Sigma Aldrich ...
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bioRxiv - Biochemistry 2023Quote: ... and a rabbit antibody recognizing a cellular marker protein (anti-TGN46, Abcam, ab50595, 1:600; anti-EEA1, Cell Signaling Technology, 2411, 1:75; anti-VPS35, Abcam, ab157220, 1:200). PLA was performed with Duolink reagents (Sigma Aldrich ...
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bioRxiv - Microbiology 2023Quote: PNPT1 and SUV3L1 proteins were detected using anti-PNPT1 antibodies and anti-SUV3L1 antibodies at 1:1000 (both obtained from Abcam, Cat #: ab176845 and Cat #: ab157109). pPKR ...
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bioRxiv - Cancer Biology 2020Quote: ... Antibody shifts were done by incubating the protein/extract with 4uL of antibody (α-Twist: Invitrogen PA5-47824, α-Snail: Santa Cruz E-18, α-Sox9: Abcam ab3667, α-E47: BD Pharmingen 554077) for 15 minutes at 25°C before adding radiolabeled probe ...