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Citations for abcam :
1001 - 1050 of 1078 citations for TSTA3 Human His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: Supernatant was harvested from HeLa tBID2A cells as described above and used to probe a Human Growth Factor Antibody Array (Abcam, ab134002) according to the manufacturer’s instructions.
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bioRxiv - Biochemistry 2020Quote: ... the protein expression was verified by transient transfection and western blot detection with goat anti-human polyclonal horse radish peroxidase (HRP) conjugated antibody (Abcam ab98567). A stable Drosophila S2 cell line for SALM3 LRR expression was then generated for the protein purification ...
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bioRxiv - Neuroscience 2021Quote: ... NT-3 was measured by standard ELISA according to manufacturer’s instructions (ab100615 – Neurotrophin 3 human ELISA Kit, abcam, Cambridge, United Kingdom).
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bioRxiv - Immunology 2021Quote: ... This spin was repeated and re-suspended in 200 μL of CBB containing FITC rabbit anti-human C3c pAb (Abcam, UK) a 500-fold dilution ...
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bioRxiv - Immunology 2021Quote: ... Grids were permeabilized and blocked with 0.1% Saponin/1% cold-water fish skin gelatin (Perm buffer) for 10 min and first stained with anti-human CD63 (Abcam, ab59479) antibody (1:10 ...
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bioRxiv - Cell Biology 2020Quote: ... Human and mouse lung sections were stained for colocalization with primary and secondary antibodies: ANT1 (Abcam #ab102032 rabbit polyclonal, 1:1000), pan ANT (anti-ANT1/2/3 Abcam ...
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bioRxiv - Cancer Biology 2022Quote: ... Mouse monoclonal anti-human-ATM(phospho-S1981) which stains the homologous mouse ph-ATM(phosphor-S1987) was used at 1:500 (Abcam ab36810). The slides were incubated in biotin-conjugated secondary antibodies ...
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bioRxiv - Cancer Biology 2022Quote: ... for 1 h and then incubated overnight at 4 °C with primary antibodies: rabbit anti-human IRS2 (ab134101; Abcam, Cambridge, UK) and mouse anti-human active β-catenin (05-665 ...
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bioRxiv - Cell Biology 2022Quote: ... Amphiregulin concentrations in HT-22 cell fractions were determined by using a Human Amphiregulin ELISA kit (ab222504; detection range: 18 – 600 pg/mL; Abcam, Netherlands). Protein concentrations were measured using RC DC Protein Assay (5000120 ...
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bioRxiv - Pathology 2023Quote: ... Sections were incubated with primary antibodies O/N at 4°C (Rabbit anti-human Lyve-1, Fitzgerald; Goat anti-murine Lyve1, R&D AF2125; rabbit anti-CD31, abcam Ab28364) and washed three times in PBS ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were then processed for immunostaining by overnight incubation at 4°C in the primary antibodies of anti-Human phospho alpha-synuclein (Ser129) (1:2000, Cat# ab51253, Abcam, RRID:AB_869973), anti-Iba-1 (1:500 ...
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bioRxiv - Bioengineering 2024Quote: ... cells were immuno-stained overnight at 4-8°C with rabbit anti-human IRF1 antibody (1:1000, ab243895, Abcam, Cambridge, UK) or rabbit anti-human pSTAT1 (1:400 ...
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bioRxiv - Immunology 2023Quote: ... was performed on the plasma samples from the donors obtained from the blood bank using Human Anti-Cytomegalovirus IgG ELISA kit (abcam, #ab108724) according to the manufacturer’s protocol to check for the donors’ sero-status ...
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bioRxiv - Cell Biology 2024Quote: ... Paraffin embedded liver sections (3 μm) from human and mouse liver samples were stained for aSMA (1:100, Abcam, Cat#ab5694), KI67 (1:50 ...
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bioRxiv - Immunology 2022Quote: ... Cells were then washed twice with PBSF and resuspended in 200 μL PBSF with 1:1000 goat anti-human antibody conjugated to AlexaFluor647 (Abcam, ab190560) and incubated for 30 min at 4 °C ...
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bioRxiv - Neuroscience 2023Quote: ... After SDS-PAGE and proteins transferred on PVDF membranes were incubated overnight at 4°C with Total OXPHOS Human WB Antibody Cocktail (Abcam, UK) and subsequently detected with HRP-conjugated goat anti-rabbit IgG secondary antibody (1:4000 ...
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bioRxiv - Molecular Biology 2023Quote: ... Membranes were blotted overnight at 4°C with the following primary antibodies: total OXPHOS human antibody cocktail (Abcam, 1:5000 dilution), TOM20 (Santa Cruz Biotechnology 1:5000) ...
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bioRxiv - Neuroscience 2023Quote: ... cryosections (50 μm) of skin were stained with primary antibodies: mouse anti human IAPP antibody (1:500, Abcam Ab115766 mouse monoclonal), rabbit anti-I11 oligomer antibody (1:500 ...
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bioRxiv - Neuroscience 2022Quote: ... at 1:250 and MJFR14 [a rabbit monoclonal antibody that has been reported to recognise assembled human α- synuclein (15)] (Abcam) at 1:1,000 ...
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bioRxiv - Neuroscience 2023Quote: ... 300μg of protein was used for immunoprecipitation assay using immunoprecipitation kit containing Dynabeads-coupled protein G (magnetic beads) and human anti-MMP9 (1:700) (Abcam, ab38898). Briefly the protein was mixed with anti-MMP9 antibody and incubated overnight at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... cryosections (10 μm) of DRG were stained with primary antibodies: mouse anti human IAPP antibody (1:500, Abcam Ab115766 mouse monoclonal), rabbit anti-I11 oligomer antibody (1:500 ...
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bioRxiv - Bioengineering 2023Quote: ... after which the media in all four reservoirs was collected and analyzed with the Human Cytokine Antibody Array (Abcam, Cambridge, UK). The full list of cytokines tested in the kit is found in supporting Table S2.
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bioRxiv - Biophysics 2023Quote: ... Wells were rinsed with PBS and incubated overnight with 50 μg/mL anti-human CD45RA antibody (Abcam ab212774, lot GR3365431-3), at 4°C.
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bioRxiv - Cancer Biology 2022Quote: Protein array analysis was performed using the Human MAPK Phosphorylation Antibody Array (ab211061) according to the manufacturer’s instructions (Abcam, Boston, MA). Visualization was performed with the ChemiDoc Touch Imaging System (Bio-Rad ...
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bioRxiv - Physiology 2023Quote: ... and human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CM)21,22 were used to study the effects of α-MSH (abcam, # ab120189) and selective MC-R agonists in vitro ...
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bioRxiv - Cell Biology 2023Quote: ... Rabbit polyclonal anti-clathrin heavy chain (against residues 1650 to C-terminus of human clathrin heavy chain, IF 1:150) was from Abcam (#ab21679). Mouse monoclonal anti-clathrin light chain (clone X22 ...
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bioRxiv - Cell Biology 2024Quote: ... Secondary antibodies and dilutions for human muscle cross section imaging: Donkey anti-mouse (Alexa Fluor® 488) 1:500 (Abcam, #ab150105), donkey anti-goat (Alexa Fluor® 594 ...
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bioRxiv - Cancer Biology 2024Quote: The evaluation of human MMPs (Matrix Metalloproteinases) and TIMPs (Tissue Inhibitors of Metalloproteinases) was conducted following the instructions provided by the manufacturer (abcam 134004). In summary ...
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bioRxiv - Neuroscience 2024Quote: The activity of CatB was examined in the cortex of 3-month-old mice or human fibroblasts using a Cathepsin B Activity Assay Kit (Abcam, #ab65300). The tissue or fibroblasts were homogenised in CB lysis buffer and then incubated on ice for 30 minutes before being centrifuged at 15,000 x g for 5 minutes at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... diluted 1:33 in BSA 1% in PBS without Ca2+ and Mg2+ or with monoclonal rabbit anti-Human ACE2 (cat. ab108252, Abcam, Cambridge, UK) diluted 1:100 in BSA 1% in PBS without Ca2+ and Mg2+ ...
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bioRxiv - Immunology 2021Quote: ... confluent monolayers of 293T Lenti-X cells were stained with 2G12 or PGT145 bNAbs and Goat Anti-Human IgG H&L (10 nm Gold) secondary Ab (Abcam, Cambridge, UK) and then fixed in 2.5% glutaraldehyde in cacodylate buffer 0.1 M ...
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bioRxiv - Molecular Biology 2020Quote: ... Unbound antibody was removed by five 5-min washes and the membrane was incubated with alkaline phosphatase-conjugated goat anti-mouse secondary antibody or goat anti-human IgG secondary antibody (Abcam, Cambridge, UK). Membranes were washed again and then developed using SuperSignal ELISA Pico Chemiluminescent Substrate Kit (Thermo Fisher Scientific).
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bioRxiv - Microbiology 2019Quote: The detection of specific anti-RSV IgG antibodies in biological fluids was performed using Human Anti-Respiratory syncytial virus IgG ELISA Kit (ab108765, abcam®, Sweden), according to manufacturer’s protocol ...
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bioRxiv - Biochemistry 2019Quote: ... Cells were then washed once with 200 μL PBS containing 1% BSA and incubated with PE conjugated Goat F(ab’)2 Anti-Human IgG-(Fab’)2 secondary antibody (abcam, Cambridge, MA) at a final concentration of 2.5 μg/ml in 100 μL PBS containing 1% BSA per well ...
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bioRxiv - Microbiology 2020Quote: The IL-12p40 in cell supernatants of infected and uninfected macrophages were quantified using BD OpteEIA™ Human IL-12(p40) ELISA kit (Abcam, UK) as per the manufacturer’s directives.
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bioRxiv - Immunology 2020Quote: ... Reacted mAbs were detected using ALP-conjugated Goat F(ab’)2 Anti-Human (IgG (Fab’)2) secondary antibody (Abcam, ab98532, 1:2000) for 30 min at RT ...
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bioRxiv - Bioengineering 2020Quote: Albumin secretion was quantified in Liver-Chip effluent collected from the top channel using the Human Albumin SimpleStep ELISA® Kit (Abcam, #ab179887) according to the manufacturer’s protocol.
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bioRxiv - Neuroscience 2022Quote: ... Primary antibodies (Goat anti-GFP biotin conjugated, Vector Labs; sheep polyclonal anti-human-sema3f antibody, R&D systems; mouse anti-GADPH, Abcam) were diluted in the blocking buffer ...
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bioRxiv - Microbiology 2021Quote: ... Expression of each species’ ACE2 receptor in the cells was confirmed by Western Blot analysis using antibody to human ACE2 (Abcam, Waltham, MA). Parental CRFK cells served as a control (Mock).
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bioRxiv - Microbiology 2021Quote: ... Chuncheon-si, ROK), anti-HCoV-OC43(LS-C79764, LS-bio, Seattle, WA, USA) and anti-human GAPDH antibody (ab9485, Abcam, Cambridge, UK) overnight at 4°C and then incubated with anti-mouse or rabbit IgG-HRP conjugate (209-035-082 or 309-035-003 ...
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bioRxiv - Bioengineering 2021Quote: ... and 1% penicillin/streptomycin in PBS (Ca2+,Mg2+)) containing 30 µg/mL Goat anti-human IgG H&L (Dylight® 488, Abcam ab96911) at a 1:1 ratio ...
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bioRxiv - Immunology 2021Quote: ... After 1 hour the wells were washed and Ab binding was detected with Donkey F(ab’)2 Anti-Human IgG conjugated to phycoerythrin (Abcam cat.# ab102439). Cells were analyzed using an iQue High-Throughput Flow Cytometer (Sartorius ...
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bioRxiv - Immunology 2022Quote: Binding of TIM-4 to infected cells was tested using a TIM-4 Fc chimera composed of the extracellular domain of TIM-4 fused to the N-terminus of the Fc region of human IgG (Abcam, Waltham, MA). One μg of TIM-4 in PBS containing 1% BSA was incubated on ice for 30 minutes with 105 washed CD4 T cells infected as indicated above with SIVGAGGFP ...
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bioRxiv - Cell Biology 2019Quote: The same protocol was used for the staining of VEGF-A expressing cells using a rabbit anti-human VEGF-A monoclonal antibody (ab52917, Abcam, Cambridge, UK), which was revealed using the same secondary antibody as above ...
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bioRxiv - Biochemistry 2019Quote: ... 20 μg of total cell lysates were separated on SDS-PAGE and protein transferred to nitrocellulose membranes that were probed with antibodies against human pancreatic α-amylase (Abcam #ab21156) and ENT2 (Alomone Labs #ANT-052 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... a pair of monoclonal antibodies (clone EPR16588, ab178846; clone EPR16589, ab178847) and a recombinant human protein (ab105593) are available from Abcam (Waltham, MA). Similarly ...
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bioRxiv - Immunology 2020Quote: TNF-α concentrations in undiluted or appropriately diluted supernatants were determined by ELISA using the SimpleStep human TNF alpha ELISA kit (abcam, Cambridge, MA), performed following the manufacturer’s instructions and with endpoint absorbance measurements at 450 nm on an Infinite M200 Pro plate reader (Tecan ...
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bioRxiv - Immunology 2021Quote: ... cells were incubated with heat-inactivated serum and visualized with a goat anti-human secondary antibody (IgG) conjugated with FITC (Abcam, Cat#ab97224).
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bioRxiv - Genetics 2020Quote: ... and incubated with rabbit polyclonal anti-PEA-15 (C-terminal amino acids 93-123 of Human PEA15) antibody ab135694 (Abcam, Cambridge, UK) at a concentration of 1:100 and anti-GAPDH antibody (MAB374 ...
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Apicosome: newly identified cell-type-specific organelle in mouse cochlear and vestibular hair cellsbioRxiv - Neuroscience 2022Quote: The following primary antibodies were used in this study: two rabbit polyclonal antibodies against human fibronectin leucine-rich transmembrane protein 1 (FLRT1) (ab97825 and ab103839; Abcam, Cambridge, UK), rabbit polyclonal anti-human FLRT2 (ab154023 ...