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Citations for abcam :
951 - 1000 of 1135 citations for IL 13 Human HEK293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2022Quote: ... Primary antibody dilutions were prepared in 3% NGS in 1XPBS as follows: mouse anti-human vinculin (VIN-54, Abcam) at 1:200 and phalloidin conjugated with Alexa Fluor 546 (Invitrogen) ...
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bioRxiv - Microbiology 2021Quote: ... The cells were resuspended with 200 μl of 1%BSA-PBS and 0.25 mg/ml of PE-labeled goat anti-human IgG (Abcam), incubated in ice for 30 minutes ...
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bioRxiv - Neuroscience 2021Quote: ... [APOE4/E4]-Ki astrocytes were incubated for 24 hours with the pro-inflammatory cocktail and different doses (0.5 and 20 µg/ml) of TAGLN3 as human recombinant protein (Abcam) prior to be analysed ...
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bioRxiv - Cell Biology 2021Quote: ... eric.rubinstein{at}inserm.fr) and mouse IgG1 anti-human CD9 (clone TS9 1/100) (commercially available at Diaclone or Abcam) and then with the following secondary antibodies ...
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bioRxiv - Cancer Biology 2020Quote: ... Bead pellet was washed three times with 0.5% BSA in PBS before incubating in 1:200 rabbit anti-human NM23A (Abcam), NME2 (Abcam) ...
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bioRxiv - Biochemistry 2022Quote: ... Blocked membranes were incubated overnight at 4 °C with either an anti human frataxin monoclonal antibody (abcam, ab 110328), an anti HPRT monoclonal antibody (SC-376938) ...
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bioRxiv - Molecular Biology 2022Quote: ... Human NQO-1 (#ab28947) and HO-1 (#ab133064) protein level changes were quantified by using ELISA kits from Abcam.
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bioRxiv - Physiology 2021Quote: was performed on frozen sections (OCT embedded) of human vein segments using mouse anti-Collagen III antibody (ab7778, abcam). Briefly ...
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bioRxiv - Physiology 2020Quote: ... Desmin polyclonal rabbit anti-human antibody (Cat# ab15200, RRID: AB_301744) was used (1:200) from Abcam (Abcam, Cambridge, UK), and secondary antibody (TRITC polyclonal goat anti-rabbit ...
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bioRxiv - Neuroscience 2021Quote: 16 μg of protein extracted from frozen human tissue samples was employed in a hemoglobin sandwich ELISA (Abcam Ab157707). The same lot of this kit was employed for the analysis of all samples and the assay was performed according to the manufacturer’s instructions.
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bioRxiv - Neuroscience 2021Quote: Levels of NT3 protein in serum and muscles were assessed using a Human Neurotrophin-3 ELISA Kit (#ab100615 Abcam). In this study the viral vector encoded the human prepro-NT3 gene ...
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bioRxiv - Molecular Biology 2022Quote: ... 100 µL biotinylated recombinant human ACE2 protein (His AVI Tag, 0.25 µg/µL, 1:100) (Sinobiology) and recombinant streptavidin protein (phycoerythrin) (Abcam) were used as the primary and secondary antibodies for ACE-2 protein detection ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... analyses was performed as described in manufacturer’s protocol (Double Stain IHC Kit: M&R on human tissue; DAB & AP/Red; Cat # ab210059; Abcam).
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bioRxiv - Immunology 2020Quote: ... HT29 (human colorectal adenocarcinoma grade II) whole cell lysate was used as a positive control for TMPRSS2 (Abcam, ab3952). Full length predicated at 54 kDa and the cleavage fragment at 25 ...
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bioRxiv - Genetics 2021Quote: ... the paraffin section of human embryonic brown adipose samples was successively stained with UCP1 antibody (Abcam, ab10983; 1:500), Perilipin antibody (CST ...
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bioRxiv - Microbiology 2020Quote: ... The exact content of ACE2 in NPs was determined with a human ACE2 ELISA kit (ab235649, Abcam, Shanghai, CHN). ELISA was conducted in triplicate and repeated three times.
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bioRxiv - Immunology 2021Quote: ... incubated with HRP-conjugated goat anti-human IgG (Fab’)2 (1:5,000 in 1% nonfat dry milk-TBST; Abcam) for 1 h at RT and visualized with KwikQuant Ultra digital ECL substrate using the KwikQuant digital imager (Kindle Biosciences ...
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bioRxiv - Cell Biology 2021Quote: The amounts of secreted prolactin were quantified in the cell supernatants by the Prolactin Human ELISA Kit (Abcam, USA). The data were normalized to the total amount of protein determined by the Bradford method ...
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bioRxiv - Immunology 2022Quote: Total human IgG in the collected culture medium was measured by the quantitative SimpleStep ELISA Kit (ab195215, ABCam, UK) following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2022Quote: Circulating levels of ACE2 from plasma samples were quantified using the Human ACE2 SimpleStep ELISA® Kit (#ab235649, Abcam) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... The blots were then washed three times in PBS 0.05% Tween 20 and then incubated at room temperture with horseradish peroxidase conjugated secondary antibody diluted in PBS 0.05% (For X509: Goat-anti-human (Abcam), 1:5000 ...
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bioRxiv - Bioengineering 2024Quote: ... samples were incubated with one of the following primary antibodies (anti-PECAM-1, rabbit anti-human, cat#AB32457, Abcam, 1:100 ...
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bioRxiv - Immunology 2023Quote: Isolated human skin immune cells were treated with 10 µg/ml of recombinant HCMV-gB (Abcam, Cambridge, UK, ab43040) or 10 µg/ml of recombinant HCMV-gH (MyBioSource ...
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bioRxiv - Cell Biology 2023Quote: ... 54 subjected to Sodium dodecyl-sulfate polyacrylamide gel electrophoresis and stained with rabbit anti-human CTHRC1 antibody (Abcam, ab85739). Fibronectin detected using an anti-human antibody (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2023Quote: Immunohistochemistry (IHC) for CDK12 expression was performed with polyclonal rabbit-anti-human-CDK12 antibody (ab246887; Abcam; dilution 1:50) using the protocol described in supplementary methods section ...
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bioRxiv - Cancer Biology 2023Quote: ... This was followed by incubation overnight at 4°C with a human-specific mouse anti-nestin antibody (abcam, ab22035) in the blocking solution ...
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bioRxiv - Microbiology 2023Quote: ... mouse monoclonal anti-Human Immunodeficiency Virus Type 1 (HIV-1) p24 clone [39/5.4A] (#ab9071, Abcam, dilution 1:500). Mouse monoclonal anti-Human Immunodeficiency Virus Type 1 (HIV-1 ...
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bioRxiv - Cell Biology 2023Quote: The human RTK phosphorylation assay was performed using an antibody array membrane accordingly to the manufacturer specifications (ab193662, Abcam).
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Shear stress and very low levels of ligand synergize to activate ALK1 signaling in endothelial cellsbioRxiv - Cell Biology 2023Quote: ... Duke University] and GAPDH (mouse anti-human GAPDH, 1:10,000, 1 hr at RT; Cat: ab8245, Abcam, Waltham, MA). Primary antibodies were detected using donkey anti-rabbit IRDye® 800CW IgG (Cat ...
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bioRxiv - Bioengineering 2024Quote: ... Membrane was then incubated in 1:5000 primary antibody (HRP-conjugated Goat pAb to human IgG, Abcam, cat.: 7153) overnight at 4°C ...
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bioRxiv - Molecular Biology 2024Quote: ... Kinase assays were performed in vitro by incubating 100 ng/µl recombinant human RNAPII-CTD (Abcam, ab81834, >95% purity) for 15 min at 30°C in the presence of 50 ng/µl CDK11s/Cyclin L ...
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bioRxiv - Cell Biology 2020Quote: ... Fixed and permeabilized cells were then incubated with 1:400 dilution of Alexa Fluor 647 conjugated mouse anti-human cytokeratin 5 antibody (Abcam) for 24 hours at 4C in permeabilization buffer ...
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bioRxiv - Molecular Biology 2021Quote: ... and the bottom half was reacted against a rabbit polyclonal antibody directed to human histone H3 (Abcam, cat. no. ab1791) at 1:40,000 ...
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bioRxiv - Immunology 2021Quote: ... monoclonal rat anti-human somatostatin (dilution 1:100; MAB2358, R&D) and monoclonal rabbit anti-chromogranin (dilution 1:400, ab15160, Abcam) ON ...
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bioRxiv - Bioengineering 2019Quote: ... both rabbit anti-human (Rockland 600-401-104, 1:200, for collagen II, Abcam ab138492, 1:1500 for collagen I) were applied in 1 % NGS ...
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bioRxiv - Cell Biology 2019Quote: HeLa cells expressing MYO19-GFP and/or mchr-Miro2 constructs were assayed for endogenous MYO19 expression using a rabbit α-human MYO19 antibody (0.9μg/ml, abcam #174286). The day after transient transfection ...
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bioRxiv - Neuroscience 2021Quote: ... Primary antibodies anti-Aβ(1–16) (Clone 6E10, 1:100, #803001, Biolengend) and anti-(human)SYT11 (1:100, ab204589, Abcam) were used ...
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bioRxiv - Bioengineering 2020Quote: ... Cells were blocked with 1% BSA-0.3 M glycine in PBST and incubated with mouse anti-human integrin αvβ3 IgG (1:200, monoclonal, LM609, Abcam, ab190147) in 1% BSA-PBST overnight at 4°C ...
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bioRxiv - Molecular Biology 2020Quote: Recombinant antibody generated from the full-length SARS-CoV-2 nucleocapsid protein (product ab272852) and goat anti-human IgG (ab97225) conjugated with horseradish peroxidase (HRP) purchased from Abcam. Nickel coated clear 96-well plates were purchased from Thermo Scientific (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2020Quote: Recombinant antibody generated from the full-length SARS-CoV-2 nucleocapsid protein (product ab272852) and anti-human secondary antibody were purchased from Abcam. Proteins were resolved on a 4-12% Bis-tris gel using SDS-PAGE and transferred to a PVDF membrane (pore size 0.45 µm) ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were treated with 10 μM TG100-115 for 3 days and conditioned medium was collected as described above followed by ELISA assay using human IL11 ELISA kit (Abcam).
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bioRxiv - Biochemistry 2020Quote: ... HRP-labeled goat anti-human IgG was used at 1:1,000 dilution and the color development was with TMB ELISA Substrate (Abcam, ab171523) The experiment was performed in duplicate and the background subtraction was done by subtracting the corresponding binding signals obtained with BSA-immobilized wells ...
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bioRxiv - Neuroscience 2020Quote: ... Mouse anti-human synuclein (1:5000, Thermo Fisher Scientific; RRID:AB_1954821; Recombinant rabbit anti-phospho S129 alpha synuclein, 1:2000, Abcam 51253);the goat anti-ChAT (1:100 ...
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bioRxiv - Neuroscience 2020Quote: ... and clone #124 (human + mouse selective antibody catalogue number ab256526) were chosen for recombinant antibody generation and are now commercially available from Abcam.
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bioRxiv - Molecular Biology 2021Quote: ... Membranes were then incubated for 24 hours with the following antibodies (1:1000 v/v dilution in TBST): i) mouse anti-human OxPhos cocktail (Abcam; Cambridge ...
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bioRxiv - Cell Biology 2020Quote: ... Anti-mouse Mastl (AP14289c) was from Abgent and anti-human MASTL was generated at the CNIO (available from Abcam, ab166647). Anti-pan-B55 (sc-81606 ...
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bioRxiv - Immunology 2022Quote: ... was used to detect the presence of human CXCL10/IP-10 in the supernatants of infected or stimulated A549 cells using TMB (Abcam) as the substrate solution and 0.3 M H2SO4 as the stop solution.
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bioRxiv - Immunology 2022Quote: ... For the analysis of cell surface molecules cells were stained with APC anti-human CD48 (MEM-102; Abcam, Cambridge, UK); APC-anti human 2B4/CD244 (2-69 ...
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bioRxiv - Bioengineering 2022Quote: ... Antibodies were as follows: i) for human cells: mouse IgG1 anti-CD44/CD73/CD90/CD45/CD105/CD14/HLA-DR (all from Abcam); ii ...
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bioRxiv - Cell Biology 2022Quote: ... The following commercial polyclonal and monoclonal antisera were used (m, mouse; h, human and r, rat proteins): anti-LIMPII (ab165222) was from Abcam; anti-Calnexin (2679) ...