Labshake search
Citations for PacBio :
1 - 50 of 299 citations for rno mir 16 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2020Quote: ... single-molecule real-time (SMRT) Pacific Biosciences (PacBio) libraries were constructed using the PacBio 20-kb protocol (https://www.pacb.com/ ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Real-Time (SMRT) library using SMRTbell Express Template Prep Kit v2.0 (PacBio, CA; Cat. #100-938-900). We sheared HMW gDNA to a target DNA size distribution between 15 – 20 kb and concentrated it using 0.45X of AMPure PB beads (PacBio ...
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bioRxiv - Microbiology 2020Quote: ... single molecule real-time (SMRT) sequencing (Pacific Biosciences, PacBio, CA, USA) of the ancestor D39 isolate was performed at the Wellcome Trust Sanger Institute (Cambridge ...
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bioRxiv - Microbiology 2019Quote: ... single molecule real time sequencing (SMRT Sequel, PacBio, Menlo Park, CA). SMRT read sequences were assembled using HGAP v4 (Chin et al ...
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bioRxiv - Plant Biology 2020Quote: ... Real-Time (SMRT) cells (libraries submitted Dec 2014, University of Washington PacBio Sequencing Services ...
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bioRxiv - Microbiology 2024Quote: ... and loaded on a single-molecule real-time (SMRT) Cell 25M (PacBio). 16S rRNA full-length sequencing was performed for 30 hours on the PacBio Revio instrument.
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bioRxiv - Evolutionary Biology 2021Quote: ... followed by single molecule real-time (SMRT) sequencing on Sequel I Platform (PacBio).
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bioRxiv - Evolutionary Biology 2019Quote: ... We carried out 20-kb single-molecule real-time DNA sequencing by PacBio and sequenced the DNA library on the PacBio RS II platform ...
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bioRxiv - Microbiology 2020Quote: Bacterial DNA methylation analysis was performed by SMRT (Single molecule, real-time, PacBio) and high throughput DNA de novo sequencing ...
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bioRxiv - Microbiology 2019Quote: ... Real-Time (RSII SMRT) cells with P6/C4 chemistry on a Pacific Biosciences (PacBio) were used to generate the raw sequence data.
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bioRxiv - Bioengineering 2023Quote: ... 2 weeks later and subjected to large deletions detection (PCR and PacBio sequencing). Single cells were isolated from bulk edited cells into 96-well plates 2 weeks after electroporation and genotyped 2 weeks after isolation ...
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bioRxiv - Molecular Biology 2019Quote: ... full length cDNA libraries were subjected to single molecule real time (SMRT) RNA-seq (PacBio platform) or conventional short read RNA-seq of fragmented cDNAs at 20 million (T ...
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bioRxiv - Plant Biology 2020Quote: ... A total of 24 Single-Molecule Real-Time (SMRT) cells (9 from PacBio RSII and 15 from PacBio Sequel) for Korso and 15 SMRT cells (all from PacBio Sequel ...
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bioRxiv - Plant Biology 2020Quote: ... A total of 24 Single-Molecule Real-Time (SMRT) cells (9 from PacBio RSII and 15 from PacBio Sequel) for Korso and 15 SMRT cells (all from PacBio Sequel ...
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bioRxiv - Genomics 2021Quote: ... 16 complete genomes by PacBio and one reference genome GCA_001549955.1 (sequenced by 454 GS-FLX ...
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bioRxiv - Genomics 2019Quote: ... and sequencing was performed at the Arizona Genomics Institute (AGI; Tucson, USA) using a Single-Molecule Real-Time (SMRT) DNA sequencing system available from PacBio. De novo assembly was performed with the PacBio Corrected Reads (PBcR ...
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bioRxiv - Evolutionary Biology 2021Quote: We sequenced the GFP coding sequence from plasmid libraries isolated after every round of evolution using single molecule real time (SMRT) sequencing(Pacific Biosciences, PacBio).
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bioRxiv - Microbiology 2020Quote: ... and sequenced at Washington State University core facility using one cell of the single molecular real time (SMRT) Pacific Biosciences (PacBio) RSII system.
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bioRxiv - Genomics 2022Quote: ... The F1 turkey was sequenced by Dovetail Genomics using PacBio singlemolecule real-time (SMRT) sequencing technology (PacBio Sequel System, RRID:SCR_017989) with a total depth of 270X ...
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bioRxiv - Evolutionary Biology 2023Quote: ... were subsequently constructed for single-molecule real-time (SMRT) sequencing to generate long high-fidelity (HiFi) reads with the Pacific Biosciences (PacBio) Sequel II system at the West German Genome Sequencing Center of HHU Düsseldorf.
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bioRxiv - Genomics 2021Quote: ... South Korea) on the RSII platform using single molecule real time (SMRT) long read sequencing technology from Pacific Biosciences (PacBio, CA, USA). The sequencing chemistry included the 20Kb SMRTbell template and the polymerase binding kit P6 Version 2 ...
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bioRxiv - Microbiology 2021Quote: ... DNA extraction and 16S rRNA full-length sequencing (PacBio) were performed as reported previously [17] ...
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bioRxiv - Genomics 2023Quote: ... We performed transcriptome assembly using StringTie v2.1.2 [16] from PacBio high-quality transcript alignments ...
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bioRxiv - Genomics 2021Quote: ... and 16 genomes from NCBI (10 of which were sequenced by PacBio). Raw reads of Illumina sequencing were assembled using Skesa v2.4.0 [22].
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bioRxiv - Immunology 2022Quote: ... 16-nucleotide barcodes were associated with their BA.1 variants by PacBio sequencing ...
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bioRxiv - Genomics 2022Quote: ... and 142X (the median depth of real PacBio data used to predict SVs using long-read tools) ...
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bioRxiv - Genomics 2020Quote: ... The polymerase-bound library was sequenced on 8 SMRT Cells with a 10 h movie time using the Sequel Sequencing Kit 3.0 (PacBio, 101-597-900) and a Sequel SMRT Cell 1M v3 (PacBio ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Motif and methylation detection were performed using the pipeline “pbsmrtpipe.pipelines.ds_modification_motif_analysis” from PacBio SMRTLink 6.0 ...
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Comprehensive fitness landscape of SARS-CoV-2 Mpro reveals insights into viral resistance mechanismsbioRxiv - Molecular Biology 2022Quote: ... with a 0.4-hour pre-extension time (PacBio Core Enterprise ...
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bioRxiv - Microbiology 2020Quote: ... the number of intervening days between serial isolations, time point (A or B, being earlier and later time points respectively) and sequencing technology (Illumina [I] or PacBio[P]) (Figure 3B) ...
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bioRxiv - Neuroscience 2021Quote: ... The SMRT sequencing was performed on the PacBio Sequel at the Genomics Core at KU Leuven (Belgium) using the Clontech SMARTer PCR cDNA Synthesis Kit (recommended by Pacbio). Multiple parallel PCR reactions were set after the first strand synthesis employing the PrimeSTAR GXL DNA Polymerase kit ...
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bioRxiv - Microbiology 2024Quote: ... The resulting genome was polished three times using Quiver (PacBio) before being error corrected with Illumina reads using Snippy v3.2 (https://github.com/tseemann/snippy) ...
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bioRxiv - Immunology 2024Quote: ... the 16S rRNA gene amplicons were generated with the barcoded Full-Length 16S rRNA gene primers (forward: AGRGTTYGATYMTGGCTCAG, reverse: RGYTACCTTGTTACGACTT) from PacBio and the 2x Roche KAPA HiFi Hot Start Ready Mix ...
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bioRxiv - Plant Biology 2022Quote: ... a run movie time of 600 min and Software v6.0 (PacBio).
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Genomic local adaptation of a generalist plant species to pollinator communities and abiotic factorsbioRxiv - Plant Biology 2022Quote: ... with 16 threads of freebayes running in parallel across regions of 100kb in the de novo polished genome assembly (PacBio, Illumina and Bionano). Single nucleotide polymorphisms (SNPs ...
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bioRxiv - Genetics 2020Quote: ... We skipped the misjoin detection step due to its high false-positive rate in the contigs derived from PacBio long reads ...
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bioRxiv - Microbiology 2021Quote: DNA modification detection and motif analysis were performed using the PacBio SMRT Link software (PacBio, Menlo Park, CA, USA). Briefly ...
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bioRxiv - Genetics 2019Quote: Another possible source for mapping bias are the errors in genome assembly and the incoordination between the assembly and the real genotype in hybrid (the YPS128 strain’s Pacbio long-reads used in assembly is not from this project) ...
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bioRxiv - Microbiology 2021Quote: ... PCR products were purified using AMPure®PB beads (PacBio), following manufacturer’s guidelines ...
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bioRxiv - Microbiology 2022Quote: ... PCR product pools were purified using AMPure®PB beads (PacBio). Sequencing was performed at the Functional Genomic Center Zürich ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Using SMRTBell template Prep Kit 1.0 and SMRTbell Barcoded Adaptater kit 8A or 8B kits (PacBio), samples (1 µg ...
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bioRxiv - Genomics 2023Quote: ... We sequenced the library on a single SMRT Cell (30 hours movie time) with the Sequel II system (PacBio) using the Binding Kit 2.0 ...
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bioRxiv - Genomics 2020Quote: ... Sequencing was performed on a PacBio Sequel instrument using the Sequel® Binding Kit and Internal control Kit 3.0 and the Sequel® Sequencing Kit 3.0 (PacBio). Long read data was assembled using the HGAP4 assembler.
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bioRxiv - Genomics 2023Quote: ... and the Express Template Prep Kit v2 and SMRTbell Cleanup Kit v2 (PacBio). The library was size-selected on a PippinHT instrument (Sage Science ...
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bioRxiv - Microbiology 2021Quote: ... PCR chemicals and conditions (27 cycles) were performed as suggested by Pacific Biosciences (PacBio, 2020). PCR products were visualized on an 1 % (w/v ...
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bioRxiv - Microbiology 2021Quote: ... SMRTbell libraries were prepared using the SMRTbell Express Template Prep Kit 2.0 (PacBio kit). Long read data were assembled by canu 2.1.1(44) ...
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bioRxiv - Microbiology 2024Quote: ... The amplicon library was contracted with the SMRTbell® prep kit 3.0 library prep kit (PacBio) following the protocol ...
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bioRxiv - Plant Biology 2021Quote: ... A Template Prep Kit (PacBio, USA) was used to remove failed ligation products ...
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bioRxiv - Microbiology 2019Quote: ... The SMARTbell Template Prep Kit (PacBio) was used to add adaptors to amplicons ...
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bioRxiv - Microbiology 2019Quote: ... the 20 kb BluePippin kit (PacBio) was used and DNA libraries were sequenced on a PacBio Sequel System with Sequel Sequencing chemistry 2.1 ...