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Citations for PacBio :
1 - 50 of 339 citations for Two Hybrid System Construction kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2023Quote: ... The two assemblies (hybrid and PacBio only) were then combined using QuickMerge v0.2 (Chakraborty et al ...
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bioRxiv - Molecular Biology 2019Quote: ... Library construction and sequencing by PacBio Sequel (Pacific Biosciences ...
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bioRxiv - Evolutionary Biology 2021Quote: ... and SMRTbell library construction was carried out using a Template Prep Kit 1.0 (PacBio). The library was then size selected to >7kb using the BluePippin system (Sage Science) ...
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bioRxiv - Genomics 2022Quote: ... DNA library construction was performed with the SMRTbell Express Template Prep Kit 2.0 (PacBio), according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... Library construction for HIFI protocol was done using SMRTbell Express Template Prep Kit 2.0 (PacBio), followed by another round of AMPure PB Beads Purification and Bluepippin size selection (< 8kbp) ...
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bioRxiv - Genomics 2019Quote: ... PacBio library construction is done by using SMRTbell Template Prep Kit (PacBio cat#100-991-900), and sequenced using SMRTcells (PacBio cat#101-008-000) ...
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bioRxiv - Genomics 2022Quote: Genomic DNA was subjected to HiFi SMRTbell library construction using the SMRTbell Express Template Prep Kit 2.0 (PacBio), according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2019Quote: ... The sequencing library was prepared following the standard SMRT bell construction protocol (PacBio). The library was sequenced on 100 PacBio RSII SMRT cells using the P6-C4 chemistry ...
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bioRxiv - Plant Biology 2023Quote: The library construction and sequencing of FENGC products on the Pacific Biosciences (PacBio) Sequel IIe SMRT platform was performed by Interdisciplinary Center for Biotechnology Research (ICBR ...
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bioRxiv - Systems Biology 2022Quote: A hybrid database was developed that incorporated isoforms from PacBio if the gene resided in the high confidence region ...
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bioRxiv - Neuroscience 2021Quote: ... the two gtf files from PACBIO long-reads specific to telencephalon and the NFIN db reference transcripts were merged using the GffCompare tool ...
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bioRxiv - Plant Biology 2024Quote: ... Two SMRTbell libraries were constructed with the SMRTbell® prep kit 3.0 (PacBio Ref. No. 102-141-700), following the manufacturer’s protocol ...
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bioRxiv - Genomics 2020Quote: Sample cleaning of SageELF fractions and throughout SMRT bell library construction was done following the manufacturer’s protocol (PACBIO). In brief ...
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bioRxiv - Zoology 2022Quote: HMW DNA was used for library construction and sequencing on a Sequel II instrument using HiFi mode (PacBio). A target of ~20 kb fragment size was used to construct SMRTbell libraries using CCS Express Library Kit V2 ...
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bioRxiv - Genomics 2023Quote: ... we sheared 10 µg of HMW DNA to an average fragment size of of 24 kb and prepared two independent non-barcoded HiFi SMRTbell libraries using SMRTbell Express Template Prep Kit 2.0 (PacBio). We pooled the two libraries and performed size-selection with a BluePippin (SageScience ...
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bioRxiv - Genomics 2021Quote: We use the diploid assembly produced by hifiasm v0.11 using 34x coverage (two 15 kb and two 20 kb libraries) by PacBio HiFi Sequel II System with Chemistry 2.0 reads (https://github.com/human-pangenomics/HG002_Data_Freeze_v1.0#hg002-data-freeze-v10-recommended-downsampled-data-mix ...
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bioRxiv - Plant Biology 2021Quote: ... The sheared DNA was used to construct the PacBio SMRT library following the standard SMRT bell construction protocol (PacBio). Hi-C and Chicago libraries were prepared following the protocols implemented by Dovetail Genomics and sequenced on an Illumina HiSeq 4000 platform.
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bioRxiv - Genomics 2021Quote: ... Two rounds of Arrow polishing (PacBio gcpp v2.0.2) using PacBio reads ...
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bioRxiv - Genomics 2022Quote: ... Two rounds of Arrow polishing (PacBio gcpp v2.0.2) using PacBio reads ...
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bioRxiv - Evolutionary Biology 2023Quote: ... two samples were used for Pacific Biosciences (PacBio) HiFi sequencing and 21 samples for Oxford Nanopore Technologies (ONT ...
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bioRxiv - Genomics 2020Quote: ... The two fractions were recombined at equimolar quantities and library preparation performed using SMRTbell Template Prep Kit v1.0 (PacBio, USA). Sequencing was performed on the PacBio Sequel 1M SMRT cell ...
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Genomic local adaptation of a generalist plant species to pollinator communities and abiotic factorsbioRxiv - Plant Biology 2022Quote: ... This dual-enzyme hybrid scaffolding used the Bionano optical maps to scaffold polished (PacBio and Illumina) contigs.
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bioRxiv - Evolutionary Biology 2022Quote: ... DNA was shipped to Brigham Young University for quality check with pulse field capillary electrophoresis followed by CLR library construction and sequencing (PacBio Sequel II). The unique molecular yields were 38 Gb and 123 Gb ...
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bioRxiv - Genomics 2022Quote: ... The assemblies were corrected with two rounds of arrow (PacBio’s SMRT Link software release 5.0.0.6792 ...
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bioRxiv - Genetics 2019Quote: Another possible source for mapping bias are the errors in genome assembly and the incoordination between the assembly and the real genotype in hybrid (the YPS128 strain’s Pacbio long-reads used in assembly is not from this project) ...
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bioRxiv - Genomics 2020Quote: ... a combination of two sequencing experiments was analyzed (PacBio_CCS_10kb and PacBio_CCS_15kb). The mean coverage was ~29x ...
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bioRxiv - Genomics 2020Quote: ... a combination of two sequencing experiments was analyzed (PacBio_CCS_10kb and PacBio_CCS_15kb). The mean coverage was ~29x ...
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bioRxiv - Genomics 2024Quote: ... Euclidean distance was measured between any two same cells from PacBio and Illumina on a UMAP ...
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bioRxiv - Evolutionary Biology 2021Quote: ... using two RSII SMRTcells v3 and P6-C4 chemistry (PacBio, Earlham Institute provider). Each cell was sequenced using a 240-minute movie ...
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bioRxiv - Evolutionary Biology 2021Quote: We sequenced two strains (MUN-016 and TOM-007) using Pacific Biosciences (PacBio) technology and the remaining 30 using Oxford Nanopore Technologies (ONT ...
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bioRxiv - Genomics 2020Quote: ... where the two datasets are comparable (1.6 fold more reads in the PacBio dataset). The PacBio dataset for the thorny skate was one of the very first VGP datasets produced ...
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bioRxiv - Genomics 2020Quote: ... we included only transcripts that were validated by two different library preparation techniques (PacBio Iso-Seq & MinION 1D or PacBio Iso-Seq and Lexogen TeloPrime or MinION 1D and Lexogen TeloPrime) ...
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bioRxiv - Evolutionary Biology 2021Quote: Two lymphoblastoid cell lines (LCLs) per species were used for isoform sequencing experiments (PacBio Iso-seq) (human ...
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bioRxiv - Genomics 2019Quote: ... freshly-hatched second-stage juveniles (J2) were collected for two weeks and used for DNA (PacBio long reads and Illumina short read sequencing ...
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bioRxiv - Genomics 2022Quote: ... The long-read library was then sequenced on the PacBio Sequel IIe system in CLR mode using the Sequel II Binding Kit 2.2 (PacBio). The short-read library with an insert size of 350 bp was sequenced on the NovaSeq6000 platform (Illumina ...
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bioRxiv - Genomics 2019Quote: ... code at https://github.com/MariaNattestad/Assemblytics/releases/tag/v1.0) with default parameters and from two haploid de novo assemblies from PacBio. For each genome assembly ...
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bioRxiv - Molecular Biology 2023Quote: Independent preparations of rAAV were sequenced on two instruments: MinION Mk1B (Oxford Nanopore Technologies) and Sequel II (PacBio). Percent genome integrity was estimated using a custom analytical pipeline ...
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Genome of Solanum pimpinellifolium provides insights into structural variants during tomato breedingbioRxiv - Genomics 2020Quote: ... and 2) the distance between the two blast hits was largely consistent with the SV size estimated by PacBio read mapping ...
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bioRxiv - Genomics 2020Quote: ... we included only transcripts that were validated by two different library preparation techniques (PacBio Iso-Seq & MinION 1D or PacBio Iso-Seq and Lexogen TeloPrime or MinION 1D and Lexogen TeloPrime) ...
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bioRxiv - Genomics 2020Quote: ... total RNA was isolated from the abomasum pyloric with high expression of MUC6 from two heterozygous goats and sequenced by PacBio Sequel ...
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bioRxiv - Evolutionary Biology 2019Quote: ... we assembled mitogenomes using long read data from two types of third-generation sequencing technology: the RSII platform of Pacific Biosciences (PacBio) and MinIOn by Oxford Nanopore (Tusso et al ...
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bioRxiv - Genetics 2020Quote: Genomic DNA of Apibacter isolates was extracted using a bead-beating method as previously described (12). Two strains (Apibacter sp. B2966 and B3706) were sequenced with the PacBio RS (PacBio) at Nextomics Biosciences Co ...
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bioRxiv - Genomics 2020Quote: ... Large-scale PCR was subsequently performed using the optimal number of cycles and the resulting amplicons divided into two fractions and purified with 0.4X and 1X Ampure PB beads (PacBio, USA). Quantification and size distribution of each fraction was then determined using the Qubit DNA High sensitivity assay (Invitrogen ...
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bioRxiv - Genomics 2023Quote: Sequencing data were obtained using two complementary long-read sequencing technologies for the assembly of hTERT RPE-1 cells: Pacific Biosciences (PacBio) High-Fidelity (HiFi ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Running Clair3 on a model trained with R9.4 and Guppy6 reads (or alternatively with HiFi for the two PacBio sequenced individuals), we generated gVCF files for each individual and combined them into multi-sample VCF files using GATK4 (Van der Auwera and O’Connor ...
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bioRxiv - Genomics 2020Quote: A long-read sequence library was constructed using an SMRTbell Express Template Prep Kit 2.0 and sequenced on SMRT cells (1M v3 LR) in a PacBio Sequel system (PacBio, Menlo Park, CA, USA). The obtained reads (≥15 kb ...
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bioRxiv - Evolutionary Biology 2022Quote: Polishing to correct sequencing errors was performed for the two MB18102 assemblies (the SB211 assemblies did not require polishing as they were generated using PacBio HiFi reads). Two rounds of polishing were carried out simultaneously on the MB18102MAT and MB18102PAT assemblies using Illumina data from the assembled F1 individual in the program Hapo-G (allowing the correct mapping of reads to the correct haplotype) ...
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bioRxiv - Genomics 2023Quote: ... a custom origin set to position 7222 (between two tRNA molecules, tRNA-s1 and tRNA-s2, lacking both gene predictions and read coverage from the PacBio RNA sequence dataset) was used.
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bioRxiv - Genomics 2020Quote: ... and sequenced on a Sequel System (PacBio). The raw reads obtained were treated with ISO-Seq3 pipeline ...
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bioRxiv - Genomics 2021Quote: ... and sequenced on the PacBio Sequel system (PacBio). The obtained long reads were assembled with Falcon_Unzip to obtain two haplotype-resolved sequences ...