Labshake search
Citations for PacBio :
1 - 50 of 328 citations for DNA Purification kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Genomics 2023Quote: ... The sheared DNA was concentrated by bead purification using PB Beads (PacBio). The sequencing library was constructed following manufacturers protocols using SMRTbell Express Template Prep kit 2.0 ...
-
bioRxiv - Genomics 2024Quote: ... followed by DNA purification with SMRTbell® cleanup beads (PacBio Ref. No. 102158-300). 2 µL sheared DNA was used to perform overnight pulse-field gel electrophoresis while the remaining sheared DNA was stored in a 2 mL DNA LoBind® Tube (Eppendorf Cat ...
-
bioRxiv - Plant Biology 2019Quote: ... The DNA/Polymerase Binding Kit P6 (PacBio) was used for DNA synthesis after the sequencing primer annealed to the SMRTbell template ...
-
bioRxiv - Evolutionary Biology 2024Quote: ... purification and concentration before undergoing library preparation using the SMRTbell Express Template Prep Kit 2.0 (PacBio) and barcoded using barcoded overhang adapters 8A/B (PacBio ...
-
bioRxiv - Genomics 2023Quote: ... A Covaris G-Tube was then used to shear 10 µg of DNA to a size range of ∼10-30 kb followed by bead purification with PB Beads (PacBio). The HiFi sequencing library was constructed following manufacturer’s protocols using SMRTbell Express Template Prep Kit 2.0 ...
-
bioRxiv - Genomics 2023Quote: ... using the Nanobind CBB DNA extraction kit (PacBio), according to the manufacturer’s instructions with modifications ...
-
bioRxiv - Neuroscience 2023Quote: DNA was extracted from frozen pellets using the Nanobind HMW DNA Extraction kit (PacBio) following the manufacturer’s instructions ...
-
bioRxiv - Genomics 2024Quote: ... and purification of polymerase-bound SMRTbell complexes were performed using the Revio polymerase kit (PacBio, Menlo Park, CA, USA). Loading concentration for sequencing was 225 pM ...
-
bioRxiv - Microbiology 2024Quote: ... extracted DNA was prepared using the Template Prep Kit 1.0 (PacBio) and following adapter ligation DNA was size selected using a BluePippin system (Sage Biosciences ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... Purification steps were performed using AMPure PB beads (PacBio). The 32 individual samples (2 µg ...
-
bioRxiv - Genomics 2023Quote: ... Purification steps were performed using AMPure PB beads (PacBio). 13 ng of DNA was purified then sheared at 10 kb using the Megaruptor1 system (Diagenode) ...
-
bioRxiv - Genomics 2023Quote: ... Purification steps were performed using AMPure PB beads (PacBio) and 1 µg of DNA was purified and then sheared at 13 kb using the Megaruptor1 system (Diagenode) ...
-
bioRxiv - Microbiology 2023Quote: ... Purification steps were performed using AMPure PB beads (PacBio).15 μg of DNA was purified then sheared at 20 kb using the Megaruptor1 system (Diagenode) ...
-
bioRxiv - Evolutionary Biology 2024Quote: ... Purification steps were performed using AMPure PB beads (PacBio) and SMRTbell cleanup beads (PacBio) ...
-
bioRxiv - Genomics 2024Quote: ... Purification steps were performed using AMPure PB beads (PacBio) and SMRTbell cleanup beads (PacBio) ...
-
bioRxiv - Molecular Biology 2023Quote: High molecular weight DNA was extracted from frozen pellets (1 million cells) using the Nanobind HMW DNA Extraction kit (PacBio) following the manufacturer’s instructions ...
-
bioRxiv - Genomics 2021Quote: ... SMRTbell libraries were prepared using a DNA Template Prep Kit 1.0 (PacBio), and 56 20-kb SMRTbell libraries were constructed ...
-
bioRxiv - Microbiology 2021Quote: ... and the DNA libraries prepared using the SMRTbell Template Prep kit (PacBio). Hybrid assembly of Illumina paired-end reads and MinION or PacBio reads was performed using Unicycler (v.0.4.8 ...
-
bioRxiv - Genomics 2021Quote: ... Purification steps were performed using 0.45X AMPure PB beads (PacBio). A total of 10 μg of DNA was purified then sheared at 40kb using the Megaruptor system (Diagenode) ...
-
bioRxiv - Genomics 2023Quote: Purification steps were performed using 0.45X AMPure PB beads (PacBio). Thirty µg of DNA was purified to perform 3 libraries ...
-
bioRxiv - Genomics 2023Quote: ... The DNA was then selected for high molecular weight DNA using the PacBio Short Read Eliminator kit (PacBio, Menlo Park, CA, USA). Concentration was assessed using the dsDNA BR assay on a Qubit fluorometer (Thermo Fisher Scientific ...
-
bioRxiv - Genomics 2024Quote: ... DNA was extracted using either the Nanobind Tissue Big DNA kit (cell line and brain) or the Nanobind HT 1ml blood kit (blood) (PacBio). For the cell line and blood samples ...
-
bioRxiv - Genetics 2019Quote: ... SMRTbell libraries were annealed and bound to P6 DNA polymerase for sequencing using the DNA/Polymerase Binding Kit P6 v2.0 (PacBio Cat#100-372-700). Bound SMRTbell libraries were loaded onto SMRT cells using standard MagBead protocols and the MagBead Buffer Kit v2.0 (PacBio Cat#100-642-800) ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... we performed a DNA damage repair reaction followed by exonuclease treatment (ExoIII and ExoVII) using the SMRTbell DNA Damage Repair Kit (PacBio # 100-486-900) according manufacturer’s instruction ...
-
bioRxiv - Genomics 2022Quote: ... DNA library construction was performed with the SMRTbell Express Template Prep Kit 2.0 (PacBio), according to the manufacturer’s instructions ...
-
bioRxiv - Evolutionary Biology 2024Quote: ... 5µg of DNA was purified then repaired using “SMRTbell Damage Repair Kit SPV3” (PacBio) and sheared between 15kb to 20 kb using the Megaruptor 1 system (Diagenode) ...
-
bioRxiv - Bioengineering 2023Quote: ... Bead purification was performed using PB Beads (PacBio, Menlo Park, CA). A PacBio sequencing library was constructed following the manufacturer’s protocol using a SMRTbell Express Template Prep kit 2.0 and size selected on a BluePippin instrument (Sage Science ...
-
bioRxiv - Genetics 2019Quote: ... The standard MagBead sequencing protocol was followed with the DNA Sequencing Kit 4.0 v2 (PacBio Cat#100-612-400 ...
-
bioRxiv - Neuroscience 2024Quote: ... and the UHMW DNA Aux Kit (Circulomics/PacBio, NB-900-101-01, no longer available). The extracted DNA was checked using the Qubit dsDNA BR assay (Invitrogen ...
-
bioRxiv - Genomics 2023Quote: ... high molecular weight (HMW) DNA was obtained with the Nanobind plant nuclei kit (Circulomics-PacBio), either from roots or young leaves ...
-
bioRxiv - Genomics 2024Quote: ... A DNA damage repair step was performed using the « SMRTbell Damage Repair Kit SPV3 » (PacBio). A total of 9.4μg of DNA was purified and then sheared at 20 kb using the Megaruptor system (Diagenode) ...
-
bioRxiv - Genomics 2023Quote: ... and the DNA library was prepared with the SMRTbell Express Template Prep Kit 2.0 (PacBio). The prepared library was sequenced by Revio (PacBio) ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... Eluted DNA was processed in a PacBio SMRTbell template prep kit v1.0 (PacBio, #100-222-300) following the manufacture’s protocol ...
-
bioRxiv - Genomics 2020Quote: DNA sequencing libraries were prepared using the Template Prep Kit 1.0-SPv3 (PacBio, 100-991-900) according to the protocol for >30 kb SMRTbell Libraries (PacBio ...
-
bioRxiv - Genomics 2023Quote: ... Genomic DNA was prepared using the SMRTbell Express Template Prep Kit (PacBio, Menlo Park, CA, USA). Long-read sequence data were obtained using a Sequel IIe system (PacBio) ...
-
bioRxiv - Plant Biology 2021Quote: ... Purification was achieved using a 0.8× ratio of AMPure PB Beads (PacBio, USA). The final sequencing library was validated using a 2100 Bioanalyzer High-sensitivity DNA chip (Agilent Technologies ...
-
bioRxiv - Genomics 2023Quote: ... For 5µg of DNA an extra repair step with SMRTbell DAMAGE REPAIR KIT (PACBIO, 100-465-900) was performed before the one step DNA damage repair + END-repair + dA tail of double stranded DNA fragments and adapters ligation ...
-
bioRxiv - Genomics 2024Quote: ... Further selection of high molecular weight DNA was performed using the Short Read Eliminator (SRE) kit (PacBio) according to the manufacturer’s guide ...
-
bioRxiv - Genomics 2024Quote: ... the DNA went through a size selection step using a SRE Kit (PacBio, SKU-102-208-300) to remove fragments up to 25kb ...
-
bioRxiv - Cell Biology 2024Quote: ... lucifugus fibroblast line was isolated using the Nanobind ®HMW DNA extraction kit for cultured cells (PacBio). M ...
-
bioRxiv - Immunology 2020Quote: ... 5 ug of fragmented DNAs were converted to SMRTbell libraries using the SMRTbell Template Prep Kit 1.0 (PacBio). The libraries were size-selected (7-kb size cutoff ...
-
bioRxiv - Plant Biology 2020Quote: ... PacBio SMRTbell libraries were constructed from the HMW DNA using the SMRTbell Express Template Prep Kit 2.0 following the manufacturer’s protocols (PacBio). A total of 24 Single-Molecule Real-Time (SMRT ...
-
bioRxiv - Genomics 2022Quote: Genomic DNA was subjected to HiFi SMRTbell library construction using the SMRTbell Express Template Prep Kit 2.0 (PacBio), according to the manufacturer’s instructions ...
-
bioRxiv - Genomics 2024Quote: Extracted DNA was size selected using the SRE XS kit (Pacbio US, Catalogue number: SKU 102-208-200) following manufacturer’s instructions to remove fragments < 10 kb in length.
-
bioRxiv - Genomics 2021Quote: ... Long-read DNA libraries were constructed using the SMRTbell Express Template Prep Kit 2.0 (PacBio, Menlo Park, CA, USA) and sequenced on SMRT cells (1M v3 LR ...
-
bioRxiv - Genomics 2023Quote: ... The library preparation procedure is described in the protocol “Preparing HiFi Libraries from Low DNA Input Using SMRTbell Express Template Prep Kit 2.0” from PacBio. The SMRTbell library sizes and profiles were evaluated using the Agilent DNA 12000 kit on the Bioanalyzer system ...
-
bioRxiv - Genomics 2023Quote: ... HMW DNA was used to generate PacBio HiFi libraries via the Template Prep Kit v1 (PacBio, 100-259-100) or SMRTbell Express Template Prep Kit v2 (PacBio ...
-
bioRxiv - Genomics 2022Quote: ... A linked-read sequencing library was prepared using the 10X Genomics Chromium Genomic Kit (from the same DNA extraction as used for PacBio sequencing ...
-
bioRxiv - Genomics 2023Quote: ... Extractions were performed using the Nanobind Tissue Big DNA kit following the manufacturer’s instructions (Pacific BioSciences - PacBio, Menlo Park, CA). For song sparrow ...
-
bioRxiv - Genomics 2024Quote: ... DNA samples for Oxford Nanopore or PacBio long read sequencing were enriched for HMW DNA (> 25 kb) employing the Short Read Eliminator Kit (Circulomics/PacBio). Quality control was performed by determining A260/A280 and A260/A230 ratios on NanoDrop ...