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Citations for Promega :
1 - 50 of 2612 citations for pVectOZ CAT Transfection control since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... and control vector using ViaFect Transfection Reagent (Promega, Madison, WI).
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bioRxiv - Molecular Biology 2021Quote: ... Transfection was done by adding Viafect transfection reagent (Promega, Cat# E4981) with mixed plasmids drop-wise in cultured cells after 10 min incubation at room temperature then gently shaking the plate for 1 min ...
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bioRxiv - Cancer Biology 2022Quote: ... and a membrane GFP (75 ng as control for transfection) using FuGENE HD transfection reagent (1µg DNA/1µL FuGENE) (Promega, WI, USA). Next ...
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bioRxiv - Cell Biology 2020Quote: ... Fluc reporter plasmids and pRL-TK renilla luciferase (rluc) transfection control plasmid (Promega) were co-transfected into these cells using jetPRIME® transfection reagent (Illkirch-Graffenstaden ...
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bioRxiv - Genomics 2022Quote: ... Transfection efficiencies were determined in parallel by preparing transfection mixes containing 4μL FuGENE HD transfection reagent (Promega, Cat#: E2311), 96μL Opti-MEM (Life Technologies ...
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bioRxiv - Cell Biology 2022Quote: ... Transfection was performed using FuGENE HD Transfection Reagent (cat# E2311; Promega, Madison, WI, USA) in accordance with the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2020Quote: ... using the ViaFect transfection reagent (Promega Cat# E4982). The cells were transfected for 48h ...
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bioRxiv - Cancer Biology 2020Quote: ... using the ViaFect transfection reagent (Promega Cat# E4982). pcDNA4 HisMax-V5-GFP-RRBP1 was kindly deposited by Alice Ting Lab [9] ...
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bioRxiv - Cancer Biology 2022Quote: ... using the ViaFect transfection reagent (Promega Cat# E4982). The transfection efficiency was confirmed by immunoblotting against Anti-CMTM6 and Anti-DDK ...
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bioRxiv - Neuroscience 2023Quote: ... using a Fugene transfection reagent (Promega; Cat# PRE2311). The medium was replaced the next day and lentivirus collected 36 h later ...
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bioRxiv - Microbiology 2021Quote: ... In vitro transfections were performed with FuGENE®6 Transfection Reagent (Promega, cat. no E2691) as described previously (Hong et al. ...
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bioRxiv - Cancer Biology 2023Quote: ... All transfections were performed using Fugene-6 transfection reagent according manufacturers protocol (Promega, cat E2691).
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bioRxiv - Genetics 2019Quote: ... The transient transfection were performed by using FuGene HD transfection reagent (Cat. No. E2311, Promega, USA).
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bioRxiv - Genomics 2023Quote: ... and 5ng of Renilla luciferase control (Promega Cat E2231). Luciferase activity was measured 48-hours post-transfection with the Dual-Luciferase Reporter Assay System (Promega Cat E1910) ...
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bioRxiv - Cell Biology 2019Quote: ... Plasmid transfections were performed with FuGENE 6 (Promega, cat#E2691) according to manufacturer instructions ...
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bioRxiv - Neuroscience 2020Quote: ... the transfection was conducted using FuGENE6 kit (Cat#2619, Promega) according to the manufacturer’s protocol.
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bioRxiv - Cell Biology 2023Quote: ... using fuGENE HD™ transfection reagent (Promega, Cat. no. E2312) following manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2019Quote: ... The luminescence values were transfection normalized with the internal control pRL-TK (50 ng, Renilla Luciferase; Promega). Experiments were performed with three biological replicates.
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bioRxiv - Neuroscience 2023Quote: ... the transfection was performed at 9 DIV using the ProFection® Mammalian Transfection System from Promega (Cat#E1200). At 15 DIV ...
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bioRxiv - Neuroscience 2021Quote: Neuronal transfections were performed on DIV 7 using a calcium phosphate kit (ProFection Mammalian Transfection System, Cat # E1200, Promega), based on a previously described method (Sando et al. ...
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bioRxiv - Molecular Biology 2020Quote: ... 293T cells were transfected in 12 well plates with 1 ug of pcDNA3.4 POLGARF or pcDNA3.4 Timer (as a negative control) with Fugene transfection reagent (Promega) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were co-transfected with 50 ng of the Renilla luciferase transfection control plasmid pRL-CMV (Promega #E2261) and 1 μg of either the ERα reporter plasmid XETL (here referred to as ERE-Luc ...
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bioRxiv - Cell Biology 2020Quote: S2R+ cell transfections were performed using FuGENE HD (Promega, cat# E2311) in 6 well plates or 25-cm2 flasks ...
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bioRxiv - Cancer Biology 2022Quote: ... Plasmid pCMV6-XL5-LAMP2A was purchased from Origene (Cat nr #: SC118738) and used for transfection with ViaFect from Promega according to the manufacturers recommended procedure ...
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bioRxiv - Microbiology 2023Quote: ... lentiviral vectors were introduced by Fugene HD transfection (Promega, Cat. #E2311) according to the manufacturer protocols ...
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bioRxiv - Cell Biology 2022Quote: Lipofectamine 3000 or FuGENE 6 transfection reagents (ThermoFisher Scientific/Invitrogen, cat# L3000008; and Promega, cat# E2691) were used for transient delivery of PRR14 plasmids ...
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bioRxiv - Zoology 2020Quote: ... K562 cells were transfected with 0.1 μg of Renilla luciferase expression plasmid pRL-TK (internal control for normalizing transfection efficiency; Promega) and 0.4 μg of abcb4 promoter construct ...
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bioRxiv - Immunology 2023Quote: ... pI.18-3xFlag-myRIG-I or pI.18-3xFlag-roRIG-I together with transfection control pLR-SV40-Renilla (Promega) and either of the reporter plasmids ISG54-Luc (69) ...
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bioRxiv - Systems Biology 2024Quote: ... FuGene 6 (cat#: E2692) transfection reagent and sequencing grade modified trypsin (cat#: V5111) were purchased from Promega. Cas9 endonuclease was purchased from Integrated DNA Technologies ...
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A CRISPR activation screen identifies FBXO22 as an E3 ligase supporting targeted protein degradationbioRxiv - Biochemistry 2023Quote: ... FuGene 6 (cat#: E2692) transfection reagent and sequencing grade modified trypsin (cat#: V5111) were purchased from Promega. Cas9 endonuclease was purchased from Integrated DNA Technologies.
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bioRxiv - Microbiology 2020Quote: ... transfections were performed in triplicate by co-transfection of the pHMRluc Rem-responsive construct and a Rem-non-responsive firefly luciferase reporter construct (pGL3-Control; Promega), in the presence and absence of a Rem-expression plasmid ...
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bioRxiv - Molecular Biology 2019Quote: ... Virus-like particles (VLPs) containing the GFP control or targeting sgRNAs were generated by transfection of HEK293 cells with FuGene (Promega). VLPs were collected from culture supernatant ...
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bioRxiv - Genomics 2022Quote: ... Firefly (transfection control) and Renilla (target) luciferase activities were sequentially measured using the Dual-Glo Luciferase Assay System (Promega E2920) and detected on a Spectramax M plate reader (Molecular Devices) ...
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bioRxiv - Immunology 2023Quote: ... pI.18-3xFlag-myRIG-I or pI.18-3xFlag-roRIG-I (25 ng/well each) together with transfection control pLR-SV40-Renilla (Promega) (50 ng/well ...
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bioRxiv - Genetics 2024Quote: ... Cells were co-transfected after 24 hours with 50 ng of the CNTNAP5 luciferase reporter constructs along with 10 ng of pRL-TK Renilla luciferase internal control using FuGENE HD transfection reagent (Promega). At 48 hours post-transfection ...
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bioRxiv - Zoology 2020Quote: ... pGL3-control plasmid (positive control; Promega), or pGL3-enhancer plasmid (negative control ...
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bioRxiv - Cancer Biology 2020Quote: ... in a ratio of 100:1 using ViaFect transfection reagent (Promega Cat# E4982). Seven TCF/LEF-binding sites are present upstream of a firefly luciferase gene in the TOPflash vector ...
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bioRxiv - Bioengineering 2022Quote: ... was purchased from Mybiosource.com. Fugene HD Transfection Reagent (Cat. #: E2311) was purchased from Promega (Madison, WI). 96-well PCR plates (Cat ...
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bioRxiv - Molecular Biology 2023Quote: ... Transfection was carried out with Fugene HD (Promega, Cat. No. E2311, Madison, WI) or Invitrogen Lipofectamine 2000 Transfection Reagent (Cat ...
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Receptor-like role for PQLC2 amino acid transporter in the lysosomal sensing of cationic amino acidsbioRxiv - Cell Biology 2020Quote: ... Transfections and co-transfections were performed using FuGENE 6 transfection reagent (Promega). Cells were analysed two days post-transfection.
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bioRxiv - Genetics 2022Quote: ... was transiently transfected with the pRL-TK Renilla internal control vector (50 ng) using 4 μL Viafect™ transfection reagent (Promega) into suspension cell lines or adherent cell lines ...
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bioRxiv - Developmental Biology 2021Quote: ... or experimental constructs along with the ptcΔ136-GL3 luciferase reporter construct and beta-galactosidase transfection control (pSV-β-galactosidase; Promega, E1081).
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bioRxiv - Cell Biology 2019Quote: ... We then assessed cell viability of treated and control cells 48 hours post-transfection using the CellTiter Glo 2.0 luciferase assay kit (Promega, Madison, WI) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: HeLa cells were transfected with plasmids encoding ByeA or ByeAC197A according to the FuGENE transfection protocol (FuGENE® HD Transfection Reagent, Promega Cat. E2311) as advised by the manufacturer ...
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bioRxiv - Genomics 2021Quote: ... HepG2 cells were then transfected with 1,250ng gRNA plasmid and 3,750ng of either pCMV_AncBE4max_P2A_GFP or pCMV_ABEmax_P2A_GFP [66] (FuGENE HD Transfection Reagent Cat#E2311, Promega). HepG2 cells were cultured for an additional 48 hours then green cells were isolated using flow cytometry (Aria II) ...
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bioRxiv - Molecular Biology 2020Quote: ... (49) as a negative control (2 μg of DNA per well of 6 well plate) with FuGene transfection reagent (Promega, Madison, WI) according to manufacturer’s instructions.
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bioRxiv - Developmental Biology 2019Quote: ... 10ng of hRLuc pGL4.75 control vector in 24-well plate format using FuGENE® HD transfection reagent (Promega Corporation, Wisconsin, United States). The cells were maintained in incomplete media in 25°C incubator for 8 hours after transfection and were then maintained in complete media for 48 hours ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were transfected with 10 ng of pRL_CMV vector (internal control, Cat# E2261, Promega) and 140 ng of promoter constructs ...
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bioRxiv - Molecular Biology 2019Quote: ... whereas Control Firefly mRNA was provided with Rabbit Reticulocyte Lysate System (Promega Cat#L4960). In vitro reaction was translated for 15 minutes at 37°C and stopped with the addition of 100 µg of cycloheximide and stored on ice prior to luciferase assay.
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bioRxiv - Microbiology 2021Quote: ... and the Firefly control plasmid (pGL3-Control; Promega). After plasmids were added to the Opti-MEM ...