Labshake search
Citations for Promega :
1 - 50 of 88 citations for p53 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Genomics 2019Quote: To generate recombinant p53 we in vitro transcribed/translated human p53 with a c-terminal HA tag using a rabbit reticulocyte system (Promega). To generate fragmented genomic DNA we tagmented 50ng of human genomic DNA from MCF7 cells using the MuSeq kit (Thermo ...
-
bioRxiv - Cell Biology 2024Quote: ... was used to assay p53 transcriptional activity and pRL-CMV (Promega) was used as the transfection control ...
-
bioRxiv - Cancer Biology 2019Quote: ... cells were co-transferred with pNL(NLucP/p53-RE/Hygro) (Promega, Madison, WI) and the desired p53-expressing plasmids ...
-
bioRxiv - Molecular Biology 2021Quote: ... SmBiT34 (peptide 86, Promega) subunit was added to the C terminus of Rat Gβ1 with a 15AA polypeptide linker between them ...
-
bioRxiv - Cancer Biology 2023Quote: ... ChAP assays were performed following transfection with various p53 expression constructs using the Halo-ChIP system (Promega Corporation) according to the manufacturer’s protocol as described previously (Adduri et al. ...
-
bioRxiv - Biophysics 2021Quote: ... C1 peptide was from Promega (Madison, WI). Lyophilized ATP was from Expedeon (Cambridge ...
-
bioRxiv - Systems Biology 2020Quote: ... and an external reference peptide mix (Promega) was spiked into the sample at the concentration of 0.5 pmol/µl ...
-
bioRxiv - Cell Biology 2023Quote: ... Peptides were digested using trypsin/LysC (Promega) overnight with head-over-head mixing ...
-
bioRxiv - Biochemistry 2024Quote: ... a K562 human peptide digest standard (Promega) and E.coli peptide standard (Waters ...
-
bioRxiv - Molecular Biology 2020Quote: ... HRP Conjugate (Promega, 1:10000 in Blocking one), was added to the membrane ...
-
bioRxiv - Cancer Biology 2024Quote: ... DNA was extracted from lymphoma derived cell lines as described above and PCR amplified using sgRNA specific primers (Supplementary Table 3, p53 primers as previously described (13) and GoTaq Green Master Mix (Promega). PCR products were amplified a second time using indexing primers ...
-
bioRxiv - Cell Biology 2020Quote: ... alkylated and digested into peptides with trypsin (Promega). The resulting peptides were desalted and concentrated before mass spectrometry by the STAGE-TIP method using a C18 resin disk (3M Empore) ...
-
bioRxiv - Zoology 2020Quote: After digestion into short peptides by trypsin (Promega), the samples were analyzed using a DiNa nanoLC system (KYA Technologies ...
-
bioRxiv - Biochemistry 2022Quote: LC-MS/MS Peptide Reference Mix (V7491, Promega) in HeLa digest (Thermo Scientific ...
-
bioRxiv - Cell Biology 2023Quote: ... peptides were eluted by on-bead trypsin (Promega) digest and acidified for MS analysis ...
-
bioRxiv - Immunology 2022Quote: ... alkylated and digested into peptides using trypsin (Promega). Peptides were desalted and concentrated using Empore-C18 StageTips and eluted with 0.5% (v/v ...
-
bioRxiv - Cancer Biology 2020Quote: ... used for infection of p53-null NSCs were obtained from previously transfected DF1 chicken fibroblasts (ATCC #CRL-12203) using FuGENE 6 Transfection reagent (Promega #E2691), according to manufacturer’s protocol ...
-
bioRxiv - Cell Biology 2020Quote: ... st-Ht31 inhibitor peptide was from Promega (Madison, WI).
-
bioRxiv - Biophysics 2021Quote: ... 49 (peptide 86 and also named as HiBiT, Promega) with a 15-amino acid (15AA ...
-
bioRxiv - Plant Biology 2023Quote: ... Peptides were digested using sequencing grade trypsin (V5113; Promega) diluted in 50 mM Triethylammonium bicarbonate buffer pH 8.5 (T7408 ...
-
bioRxiv - Plant Biology 2024Quote: ... Peptides were digested using sequencing grade trypsin (V5113; Promega) diluted in 50 mM Triethylammonium bicarbonate buffer pH 8.5 (T7408 ...
-
bioRxiv - Biochemistry 2022Quote: ... Proteins/peptides were further digested with trypsin (Trypsin Gold, Promega) at 37 °C overnight ...
-
bioRxiv - Microbiology 2020Quote: ... Peptides were released by digestion with PNGase F (Promega, Madison, WI) overnight at 37 °C with vortexing ...
-
bioRxiv - Systems Biology 2022Quote: ... peptides were separated were spotted on streptavidin-conjugated membranes (Promega #V2861) where they tightly associated via their C-terminal biotinylations ...
-
bioRxiv - Biochemistry 2021Quote: ... A 15-amino acid linker and SmBiT subunit (peptide 86, Promega) were attached to the C-terminus of rat Gβ1 ...
-
bioRxiv - Developmental Biology 2022Quote: ... Purified protein was then digested into peptides using Trypsin (Promega, V5111) and desalted using Pierce C18 Spin Columns (Thermo Fisher ...
-
bioRxiv - Microbiology 2020Quote: ... and then the proteins were digested to peptides using trypsin (Promega) at a trypsin/protein ratio of 1:50 (w/w ...
-
bioRxiv - Biochemistry 2024Quote: ... and 10 units of Peptide N-glycosidase F (PNGase F, Promega) enzymes according to manufacturer’s instructions ...
-
bioRxiv - Biochemistry 2019Quote: ... PCR products for the peptide genes were subcloned into pGEM-T (Promega) and sequenced ...
-
bioRxiv - Cell Biology 2020Quote: ... LAMP1-HaloTag transfected cells were incubated with the JF646 peptide (Promega, GA1121) at 200 nM for 15 min ...
-
bioRxiv - Cell Biology 2023Quote: InCELLect™ AKAP St-Ht31 inhibitor peptide was from Promega (Chilworth, UK). Human fibrinogen was from Enzyme Research (Swansea ...
-
bioRxiv - Immunology 2023Quote: ... Tissues were permeabilized and blocked in blocking buffer with 0.05% Tween 20 (H5152, Promega) and 5% BSA (A3059 ...
-
bioRxiv - Systems Biology 2021Quote: ... Proteins were then digested to peptides with sequencing grade modified trypsin (Promega V5113), at 37 °C overnight.
-
bioRxiv - Biochemistry 2023Quote: ... and peptides were generated by in-gel digestion using trypsin (sequencing-grade (Promega)) at 37°C overnight ...
-
bioRxiv - Microbiology 2020Quote: ... The peptides were spotted onto streptavidin-coated filter sheets (Promega SAM2 biotin capture membrane) and visualized by phosphorimaging on Typhoon FLA 7000 ...
-
bioRxiv - Biochemistry 2020Quote: N-glycans were released from nMPO using Elizabethkingia miricola peptide-N-glycosidase F (Promega) (Jensen et al. ...
-
bioRxiv - Molecular Biology 2021Quote: ... Rat Gβ1 was cloned with a C-terminal SmBiT34 (peptide 86 or HiBiT, Promega) connected with a 15AA polypeptide linker ...
-
bioRxiv - Bioengineering 2020Quote: ... Peptides were then produced by trypsin digestion (50 ng trypsin, Promega Sequence Grade, Modified) (Liu et al ...
-
bioRxiv - Physiology 2020Quote: The protein solution samples were hydrolyzed into peptide segments by trypsin (V9012, Promega (Beijing) Biotech Co. ...
-
bioRxiv - Biochemistry 2021Quote: The fused peptide–mRNA was subsequently reverse transcribed using MMLV RT RNase H- (Promega) for 1 h at 42 °C and 0.5 μL aliquot of the peptide-mRNA fusions was taken from the mixture and saved for the determination of the total amount of inputted mRNA ...
-
bioRxiv - Physiology 2022Quote: ... Peptides contained C-terminal biotinylations and were spotted on streptavidin-conjugated membranes (Promega #V2861) and imaged with Typhoon FLA 7000 phosphorimager (GE ...
-
bioRxiv - Cell Biology 2023Quote: ... The proteins were then digested into peptides with sequencing grade modified trypsin (Promega V5113) at 37 °C overnight ...
-
bioRxiv - Biochemistry 2023Quote: ... peptides were collected as described in ProteaseMax application note (Promega, TB373, Rev. 2/15), and purified using Pierce C18 Spin Tips (Thermo Fisher PI84850) ...
-
bioRxiv - Biophysics 2024Quote: The fused peptide–mRNA was subsequently reverse transcribed using MMLV RT Rnase H-(Promega) for 1 h at 42 °C ...
-
bioRxiv - Biophysics 2019Quote: ... Generation of tryptic peptides for mass spectrometry was performed with the aid of ProteaseMax (Promega) detergent ...
-
bioRxiv - Biochemistry 2021Quote: ... chymotrypsin and peptide-N-glycosidase F (PNGase F) were obtained from Promega (Madison, WI, USA). Centrifugal filters (0.5 mL ...
-
bioRxiv - Molecular Biology 2020Quote: ... The membrane was probed with α-Halotag mouse monoclonal antibody (Promega, diluted 1:1000 in Blocking Buffer), washed 3 times in TBS-Tween ...
-
bioRxiv - Molecular Biology 2020Quote: ... then probed with goat α-mouse HRP-conjugated secondary antibody (Promega, diluted 1:10000 in Blocking Buffer) and finally washed 3 times in TBS-Tween ...
-
bioRxiv - Biochemistry 2022Quote: ... the primary antibody was diluted in blocking buffer as follows: Anti-HaloTag (1:1000; Promega Corporation; G9281); anti-PHC1 antibody (1:500 ...
-
bioRxiv - Biochemistry 2022Quote: ... and then peptides were collected after enzymatic digestion using trypsin (Trypsin Gold, Promega, Fitchburg, WI, USA); the peptides were desalted by Oasis HLB cartridges (Waters ...