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Citations for Promega :
1 - 50 of 5336 citations for hsa mir 296 5p Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: The sequence containing three consecutive perfect matches for hsa-miR-145-5p was excised from pSG5-Luc-3xmiR-145 (11) and sub-cloned in pMirGlo (Promega) to generate pMiRGlo 3xmiR-145.
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bioRxiv - Cancer Biology 2019Quote: A wild-type or mut-hsa-miR-329-5p fragment was constructed and inserted downstream of the luciferase reporter gene of the pmirGLO Vector (Promega, USA). We used ExFect 2000 Transfection Reagent to transfect the reporter plasmid into 293T cells ...
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bioRxiv - Cancer Biology 2021Quote: ... and real-time PCR using GoTaq 2-Step RT-qPCR kit (Promega, A6110). All measurements were normalized against Actin as the internal control using the 2-ΔΔCt method (Figure 1—source data 3 and Figure 3—source data 2) ...
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bioRxiv - Microbiology 2024Quote: Real-time RT-qPCR assays (GoTaq 1-step qRT-PCR, Promega) were performed using standard fast cycling parameters (i.e ...
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bioRxiv - Immunology 2020Quote: ... Quantitative real-time RT-PCR (qRT-PCR) was performed with the GoTaq qPCR Mastermix (Promega) using the CFX-90 real-time PCR System (Bio-Rad).
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bioRxiv - Pathology 2021Quote: ... Quantitative RT-PCR was performed by using the Go-Taq Real-Time PCR system (Promega), as previously described60 ...
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bioRxiv - Immunology 2023Quote: ... Quantitative real-time RT-PCR (qRT-PCR) was performed with the GoTaq qPCR Mastermix (Promega) using the CFX-90 real-time PCR System (Bio-Rad) ...
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bioRxiv - Cancer Biology 2024Quote: Quantitative real-time PCR (RT-qPCR) was performed using GoTaq PCR Master Mix (Promega, #A6002) and specific primers listed below ...
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bioRxiv - Microbiology 2021Quote: ... Viral RNA was quantified by real-time RT-qPCR (GoTaq 1 step RT-qPCR kit, Promega). Quantification was provided by serial dilutions of an appropriate T7-generated synthetic RNA standard ...
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bioRxiv - Microbiology 2022Quote: Viral RNA was quantified by real-time RT-qPCR (GoTaq 1 step RT-qPCR kit, Promega). Quantification was provided by serial dilutions of an appropriate T7-generated synthetic RNA standard ...
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bioRxiv - Microbiology 2023Quote: ... viral RNA was quantified by real-time RT-qPCR (GoTaq 1 step RT-qPCR kit, Promega). Quantification was provided by serial dilutions of an appropriate RNA standard ...
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bioRxiv - Microbiology 2024Quote: ... Viral RNA was quantified by real-time RT-qPCR (GoTaq 1 step RT-qPCR kit, Promega). Quantification was provided by serial dilutions of an appropriate T7-generated synthetic RNA standard ...
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bioRxiv - Microbiology 2022Quote: ... Viral RNA was quantified by real-time RT-qPCR (GoTaq 1-step qRt-PCR, Promega) using 3.8μL of extracted RNA and 6.2μL of RT-qPCR mix and standard fast cycling parameters ...
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bioRxiv - Microbiology 2021Quote: ... Viral RNA was quantified by real-time RT-qPCR (GoTaq 1-step qRt-PCR, Promega) using 3.8µL of extracted RNA and 6.2µL of RT-qPCR mix and standard fast cycling parameters ...
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bioRxiv - Microbiology 2022Quote: ... Viral RNA was quantified by real-time RT-qPCR (GoTaq 1-step qRt-PCR, Promega) using 3.8 µL of extracted RNA and 6.2 µL of RT-qPCR mix and standard fast cycling parameters ...
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bioRxiv - Cancer Biology 2021Quote: ... Real-time quantitative PCR (RT-qPCR) was performed with GoTaq qPCR Master Mix (Promega; Cat#: A6002). Gene expression analysis was calculated following normalization to PPAI using the comparative Ct (cycle threshold ...
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bioRxiv - Plant Biology 2024Quote: ... Real-time quantitative PCR (RT-qPCR) was performed using the GoTaq QPCR Master Mix (Promega, A6001) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... Quantitative real-time PCR was performed using the SYBR premix kit (Promega), and the primer sequences used are listed in Supplemental Table S2 ...
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bioRxiv - Cell Biology 2021Quote: ... Real time quantitative PCR was performed in a CFX 96 BioRad CFX 96 Real-Time PCR detection instrument with GoTaq qRT-PCR master mix (Promega, A6002). The sequence of the primers used in this study are ...
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bioRxiv - Synthetic Biology 2019Quote: The wild type and mutant promoter of miR-146a-5p were constructed into pGL3 vector (Promega). The pGL3-WT or pGL3-mut were co-transfected with pRL-TK using lipofectamine® 3000 ...
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bioRxiv - Neuroscience 2020Quote: ... RT-qPCR was performed on the ViiA 7 Real-Time PCR System using GoTaq qPCR master mix (Promega) according to the manufacturer’s protocols ...
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bioRxiv - Microbiology 2023Quote: Real-time PCR was carried out using the Promega GoTaq® Probe 1-Step RT-qPCR System (Promega) with Sarbecovirus-specific envelope gene primers from bat RNA samples as published by (Corman ...
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bioRxiv - Microbiology 2023Quote: Real-time PCR was carried out using the Promega GoTaq® Probe 1-Step RT-qPCR System (Promega) with Sarbecovirus-specific envelope gene primers from bat RNA samples as published by (Corman ...
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bioRxiv - Microbiology 2023Quote: ... Real time quantitative PCR was performed using GoTaq qPCR Master mix kit (Promega) in a CFX Connect Real-Time PCR thermocycler (BioRad) ...
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bioRxiv - Molecular Biology 2021Quote: ... All the reactions were performed in a CFX-Connect real-time PCR detection system (Bio–Rad, www.bio-rad.com/) using GO Taq qPCR master mix (Promega, USA). Each experiment was repeated at least three times ...
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bioRxiv - Genomics 2021Quote: ... All the reactions were performed in a CFX-Connect real-time PCR detection system (Bio–Rad, www.bio-rad.com/) using GO Taq qPCR master mix (Promega, USA). Each experiment was repeated at least three times ...
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bioRxiv - Immunology 2019Quote: ... Access RT-PCR kit (Promega) was used for characterization of recombinant influenza viruses.
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bioRxiv - Microbiology 2024Quote: ... and a Go-Taq real-time PCR system (Promega). The data were analyzed by the ΔΔCT method and normalized to ACT1 in each sample ...
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bioRxiv - Developmental Biology 2021Quote: ... Real time PCR was performed on an ABI Prism 7900 detection system using Gotaq master mix (Promega, Madison, WI, USA). Expression relative to the housekeeping gene TBP was calculated using the delta(d)Ct method and expressed as 2^(-dCT ...
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bioRxiv - Cancer Biology 2022Quote: ... Quantitative real-time PCR was performed using GoTaq PCR Master Mix (Promega) or PowerUp SYBR Green Master Mix (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2021Quote: ... quantitative real-time PCR was carried out using the GoTaq qPCR Master Mix kit (Promega) with 20 µL of reaction mixture containing gene-specific primers or the PrimePCR assay kit (Bio-Rad ...
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bioRxiv - Neuroscience 2024Quote: ... The quantitative real-time PCR was performed using the GoTaq qPCR Master Mix kit (Promega) following the manufacturer’s guidelines ...
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bioRxiv - Immunology 2023Quote: ... Quantitative RT-PCR was performed using a OneTaq RT‒PCR kit (Promega, M0482S) with qTOWER3 (Analytik Jena) ...
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bioRxiv - Microbiology 2023Quote: One step RT-PCR kit (Promega) was used ...
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bioRxiv - Developmental Biology 2019Quote: RT-PCR detection of Actn2 splicing variants was performed using GoTaq PCR master mix (Promega) and primers ...
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bioRxiv - Microbiology 2024Quote: ... Quantitative real-time PCR (qRT-PCR) was performed with the GoTaq polymerase (Promega) and a StepOnePlus real-time PCR device (ThermoFisher) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Quantitative real-time PCR was performed using GoTaq qPCR (Promega) BRYT Green Dye-based detection and CFAV NS3-specific primers ...
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bioRxiv - Immunology 2020Quote: ... and real-time PCR performed using GoTaq qPCR Master mix (Promega) on ABI QuantStudio 7 Flex Real Time PCR System ...
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bioRxiv - Immunology 2020Quote: ... Real-time PCR was performed with the GoTaq qPCR Mastermix (Promega) using the CFX-96 real-time PCR System (Bio-Rad) ...
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bioRxiv - Immunology 2024Quote: ... Real-time PCR was executed with the GoTaq qPCR Mastermix (Promega) employing the CFX-96 real-time PCR System (Bio-Rad) ...
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bioRxiv - Molecular Biology 2019Quote: Validation of hsa-miR-548ba and hsa-miR-7973 direct binding to target gene 3’UTR was performed by using Dual-Glo Luciferase assay system (Promega) according to the user manual ...
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bioRxiv - Cancer Biology 2021Quote: MAGI2-AS3 or FOXN3 3’UTR containing miR-452-5p wild-type (WT) or mutant (MUT) binding sites were inserted into psiCHECK vector (Promega, USA). 293T cells were transfected with the constructed luciferase plasmid together with miR-NC or miR-452-5p mimics using Lipofectamine 2000 ...
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bioRxiv - Cancer Biology 2021Quote: ... Real-time reverse transcription-PCR experiments were conducted with SYBR Green (Promega) in CFX Connect Detection System (1855201 ...
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bioRxiv - Neuroscience 2021Quote: ... Real-time PCR analysis was performed with GoTaq qPCR master mix (Promega) on a CFX384 system (Bio-Rad) ...
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bioRxiv - Plant Biology 2020Quote: ... Quantitative real-time PCR was performed using GoTaq qPCR Master Mix (Promega) at 58oC annealing temperature on LightCycler480 instrument (Roche) ...
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bioRxiv - Neuroscience 2021Quote: ... Real-time PCR analysis was performed with GoTaq qPCR master mix (Promega) on a CFX384 system (Bio-Rad) ...
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bioRxiv - Cell Biology 2022Quote: Real-time PCR was performed using the GoTaq qPCR Master Mix (Promega) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... Real-time PCR was performed with the GoTag qPCR Master Mix (Promega) and measured on a LightCycler480 instrument (Roche ...
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bioRxiv - Genetics 2020Quote: ... Real-time quantitative PCR was performed with GoTaq qPCR Master Mix (Promega) by Mx3000P QPCR System (Agilent Technologies) ...
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bioRxiv - Developmental Biology 2019Quote: ... Real-time PCR was performed using GoTaq qPCR Master Mix (Promega, USA) and oligonucleotide primers specifically designed to amplify the homologues of ANO1 ...