Labshake search
Citations for Promega :
1 - 50 of 2430 citations for hsa mir 150 3p RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2024Quote: ... BMSCs were transfected with miR-335-3p mimic, miR-335-3p inhibitor and NC that synthesized by RiboBio (Guangzhou, China) via Fugene HD (Promega, Madison, WI, USA; E2311) when cells confluency reached 50%.
-
bioRxiv - Molecular Biology 2020Quote: ... or miR-324-3p.1 were amplified and inserted into pmirGLO (Promega) at the XhoI and XbaI sites ...
-
bioRxiv - Neuroscience 2023Quote: ... RT-PCR with random primers (Promega, Madison, WI, USA) and Moloney murine leukemia virus M-MLV reverse transcriptase (Promega ...
-
bioRxiv - Neuroscience 2023Quote: ... RT-PCR with random primers (Promega, Madison, WI, USA) and Moloney murine leukemia virus MMLV reverse transcriptase (Promega ...
-
bioRxiv - Plant Biology 2020Quote: ... Primers were first tested by RT-PCR using Gotaq (Promega) with cycle conditions 95°C for 5 min ...
-
bioRxiv - Molecular Biology 2019Quote: Validation of hsa-miR-548ba and hsa-miR-7973 direct binding to target gene 3’UTR was performed by using Dual-Glo Luciferase assay system (Promega) according to the user manual ...
-
bioRxiv - Cell Biology 2020Quote: The sequence containing three consecutive perfect matches for hsa-miR-145-5p was excised from pSG5-Luc-3xmiR-145 (11) and sub-cloned in pMirGlo (Promega) to generate pMiRGlo 3xmiR-145.
-
bioRxiv - Cancer Biology 2019Quote: A wild-type or mut-hsa-miR-329-5p fragment was constructed and inserted downstream of the luciferase reporter gene of the pmirGLO Vector (Promega, USA). We used ExFect 2000 Transfection Reagent to transfect the reporter plasmid into 293T cells ...
-
bioRxiv - Cell Biology 2021Quote: ... of SNHG16 sequence and the 3’-untranslated region (UTR) fragment of STARD9 containing miR-1301-3p binding site were subcloned into the pmirGLO vectors (Promega, Madison, WI, USA) to generate SNHG16-Wt/Mut vectors and STARD9-Wt/Mut vectors ...
-
bioRxiv - Zoology 2024Quote: RT-PCR was performed using AccessQuick RT-PCR System (Promega, France) in total reaction volume of 25 µl with 5 µl of RNA extracts and 400 nM of forward primer (5’-ATTCGGGAGGAGACGTGGTA-3’ ...
-
bioRxiv - Developmental Biology 2021Quote: ... using either miR-430/5S rRNA specific reverse primers or random hexamers (Promega, C118A) following manufacturer’s instructions ...
-
bioRxiv - Microbiology 2019Quote: ... along with 150 ng random primers (Promega, Madison, WI). Cycling conditions were as follows ...
-
bioRxiv - Cancer Biology 2019Quote: ... RT-PCR was performed using the Access RT-PCR System from Promega. Real-time PCR was done using SYBR Green Realtime PCR Master Mix (TOYOBO ...
-
bioRxiv - Cancer Biology 2019Quote: ... RT-PCR was performed using AccessQuick™ RT-PCR system (Promega #A1700) and samples were ran on 1.2% agarose gel ...
-
bioRxiv - Immunology 2023Quote: ... Quantitative RT-PCR was performed using a OneTaq RT‒PCR kit (Promega, M0482S) with qTOWER3 (Analytik Jena) ...
-
bioRxiv - Microbiology 2023Quote: ... using M-MLV-RT and random hexamer primers (Promega) as per manufacturer’s instructions ...
-
bioRxiv - Microbiology 2023Quote: ... using M-MLV-RT and random hexamer primers (Promega) as per manufacturer’s instructions ...
-
bioRxiv - Immunology 2019Quote: ... Access RT-PCR kit (Promega) was used for characterization of recombinant influenza viruses.
-
bioRxiv - Plant Biology 2021Quote: ... Reverse transcription (RT) involved the Access RT-PCR System (Promega), as per the manufacturer’s instructions ...
-
bioRxiv - Immunology 2019Quote: ... with the addition of 150 ng random primers (Promega, Madison, WI). Cycling conditions were as follows ...
-
bioRxiv - Immunology 2023Quote: ... 100 nM probe and 150 ng random primers (Promega, Madison, WI). Reactions cycled with the following conditions ...
-
bioRxiv - Plant Biology 2020Quote: ... Quantitative real time RT-PCR (qPCR) was performed using gene-specific primers (Supplemental Table 1) and the fluorescent dye SYBR Green (Promega). All quantifications were normalized to the TIP41 (AT4G34270 ...
-
bioRxiv - Bioengineering 2019Quote: ... a specific BYDV nucleotide sequence (294 bp, primers PVinterF+YanRA) amplified by RT-PCR was inserted into the vector pGem-T Easy (Promega) and cloned into E ...
-
bioRxiv - Microbiology 2023Quote: One step RT-PCR kit (Promega) was used ...
-
bioRxiv - Pathology 2022Quote: ... The final reaction mixtures contained 150 ng random primers (Promega, Madison, WI), 600 nM each primer and 100 nM probe ...
-
bioRxiv - Immunology 2021Quote: ... The final reaction mixtures contained 150 ng random primers (Promega, Madison, WI), 600 nM each primer and 100 nM probe ...
-
bioRxiv - Pathology 2022Quote: ... The final reaction mixtures contained 150 ng random primers (Promega, Madison, WI), 600 nM each primer and 100 nM probe ...
-
bioRxiv - Pathology 2023Quote: ... The final reaction mixtures contained 150 ng random primers (Promega, Madison, WI), 600 nM each primer and 100 nM probe ...
-
bioRxiv - Developmental Biology 2021Quote: PCR (RT-PCR) was conducted using GoTaq DNA polymerase (Promega). PCR programs were the following ...
-
bioRxiv - Cancer Biology 2020Quote: ... with the primers sets (BE3_PCR_F and BE3-PCR_R) using Pfu DNA polymerase (Promega, M7741). Amplified fragments were digested with XhoI and NotI and cloned into pCAGIG plasmid (Addgene ...
-
bioRxiv - Biophysics 2021Quote: ... using the Access RT-PCR System (Promega), and then exchanged with exon 6b in mH1 using the Quikchange method of site-directed mutagenesis (SDM ...
-
bioRxiv - Cancer Biology 2022Quote: ... These cells were transfected with the pcDNA6.2-GW /EmGFPmiR plasmids containing miR sequences (miR-NRF2 #1, #2, #3, #4 and miR-ctl 79 using the FUGENE HD transfection reagent (Promega #E2311). Seven days later GFP positive cells were isolated by flow cytometry (Biorad S3e cell sorter) ...
-
bioRxiv - Cancer Biology 2023Quote: ... Quantitative RT-PCR was performed with GoTaqβ qPCR and RT-qPCR Systems (Promega) on a CFX384 Real-time System (Bio-Rad) ...
-
bioRxiv - Molecular Biology 2023Quote: ... SYBR Green-based RT-qPCR reactions were set up using GoTaq qPCR Master Mix (Promega) and 20 ng of input cDNA per each 10-μL reaction ...
-
bioRxiv - Microbiology 2023Quote: Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) system for RT-PCR (Promega) was used for cDNA synthesis using 500 ng extracted RNA from all samples ...
-
bioRxiv - Microbiology 2021Quote: ... A series of concentrations from 50-600nM in symmetric and asymmetric forward and reverse primer concentrations were added to the RT-qPCR reaction using the GoTaq 1-Step RT-qPCR System (Promega) according to the manufacturer’s instructions using the standard annealing temperature of 60°C ...
-
bioRxiv - Genomics 2022Quote: ... and cDNA synthesis using the GoScript RT Kit and oligo dT primer (Promega). To amplify the random 5’ and 3’ BC from the plasmid DNA or cDNA sample ...
-
bioRxiv - Biochemistry 2021Quote: ... Quantitative RT-PCR was performed using GoTaq® Probe qPCR and RT-qPCR Systems (Promega) in a StepOne™ Real-Time PCR System (Thermo Fisher) ...
-
bioRxiv - Microbiology 2021Quote: ... Quantitative RT-PCR was performed using GoTaq® Probe qPCR and RT-qPCR Systems (Promega) in an StepOne(tm ...
-
bioRxiv - Microbiology 2020Quote: ... Quantitative RT-PCR was performed using GoTaq® Probe qPCR and RT-qPCR Systems (Promega) in an StepOne™ Real-Time PCR System (Thermo Fisher Scientific ...
-
bioRxiv - Microbiology 2022Quote: ... Reverse transcriptase quantitative PCR (RT-qPCR) was run using the GoTaq RT-qPCR System (Promega) with the IDT E Assay First Line Screening primers and probe (Cat # 10006804 ...
-
bioRxiv - Cell Biology 2022Quote: ... SYBR Green-based RT-qPCR reactions were set up using the GoTaq qPCR Master Mix (Promega) and 20 ng input cDNA per each 10-μL reaction ...
-
bioRxiv - Cancer Biology 2020Quote: ... RT-PCRs were conducted in two-step reactions by using two-step RT-PCR: M-MLV reverse transcriptase in M-MLV RT-PCR system (Promega, Madison, WI) and Platinum Taq polymerase (GeneMark ...
-
bioRxiv - Molecular Biology 2021Quote: ... for RT-PCR and GoTaq qPCR Master Mix (Promega) for RT-qPCR ...
-
bioRxiv - Developmental Biology 2022Quote: ... RT (reverse transcription) PCR was performed using GoTaq (Promega). For quantitative RT-PCR analysis ...
-
bioRxiv - Genomics 2020Quote: ... RT-PCR was done with the GoTaq Polymerase (Promega) using forward primer (5’-ACGTGGAGCCCAACTTTGTA-3’ ...
-
bioRxiv - Genetics 2022Quote: ... RT-PCR was performed using GoTaq DNA Polymerase (Promega) using a T100 Thermal cycler (Bio-Rad ...
-
bioRxiv - Molecular Biology 2020Quote: ... Reverse transcription was performed with random hexamer primers using an MMLV-RT platform (Promega) according to the manufacturer’s protocol ...
-
bioRxiv - Neuroscience 2021Quote: ... For RNA retrotranscription and RT-PCR it was used GoTaq 2-Step RT qPCR System (Promega) according to the manufacturer’s instructions and it was performed using Mic qPCR Cycler (BioMolecular Systems ...
-
bioRxiv - Genomics 2021Quote: ... and cDNA synthesis using the GoScript RT kit and oligo dT primer (Promega, Madison, Wisconsin). For STARR-seq samples ...