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Citations for Promega :
1 - 50 of 1431 citations for West Nile virus 2 lysate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2024Quote: ... Virus was concentrated using PEG virus precipitation (Promega # V3011). In brief ...
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bioRxiv - Cell Biology 2022Quote: The SARS-CoV-2 spike pseudotyped virus activity was determined by bright-glo luciferase assay (Promega). The plate reader detected the luminescence two days post virus infection or without virus infection ...
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bioRxiv - Microbiology 2022Quote: ... virus was removed and cells were transfected with 2 µg of plasmid DNA pUCSP-A24Rcd using FuGeneHD (Promega) following manufacturer’s recommendations ...
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bioRxiv - Microbiology 2021Quote: ... virus inoculum was removed and cells were then transfected with 2 µg of plasmid DNA using FuGeneHD (Promega) according to the manufacturer’s recommendations ...
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bioRxiv - Immunology 2023Quote: ... 2 μg RNA was used to synthesize first-strand cDNA using Moloney mouse leukemia virus (M-MLV) (Promega). The amplification of specific PCR products was detected using SYBR Premix Ex TaqTM II (Vazyme) ...
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bioRxiv - Biochemistry 2020Quote: ... and the pre-cleared lysate was supplemented with 2 µl of RNasin Plus (Promega), added onto the freshly prepared ...
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bioRxiv - Microbiology 2021Quote: ... The cDNA was synthesized using 5μg of total RNA at 42 °C for 2 hours using avian myeloblastosis virus (AMV) reverse transcriptase (Promega). A 20 µL reaction containing 10 ng or 10 pg (for 16S rRNA ...
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bioRxiv - Microbiology 2023Quote: Cells infected with a luciferase-encoding virus were lysed 2 days after infection with a Bright-Glo Luciferase Assay System (E2620, Promega) and the luminescent signal was measured using a GloMax Explorer Multimode Microplate Reader (Promega).
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bioRxiv - Developmental Biology 2021Quote: ... and the resulting mRNAs (2 μg) were translated in 50 μl of rabbit reticulocyte lysate (Promega). Flag-tagged proteins were purified by immunoprecipitation with anti-Flag M2 antibody (Sigma ...
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bioRxiv - Microbiology 2020Quote: ... The lysate was then diluted to <2 M urea and incubated with 50 μg trypsin (Promega) overnight at 37 °C under agitation ...
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bioRxiv - Genetics 2022Quote: ... The lysate was digested overnight at 37 °C with 2 μg of sequencing-grade trypsin (Promega), after which the sample was acidified to 0.1% formic acid ...
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bioRxiv - Cell Biology 2021Quote: ... The primary MEFs were immortalised by transfection of 2 μg of simian virus 40 large T-antigen-expressing vector employing Fugene 6 Transfection Reagent (Promega, E2311) followed by five rounds of a 1 in10 split to achieve 1/100,000-fold splitting ...
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bioRxiv - Biophysics 2020Quote: ... Virus infectivity was determined by luciferase production (Promega) after 48h using a Synergy2 Multi-Detection Microplate Reader (BioTek)
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bioRxiv - Microbiology 2020Quote: ... virus replication was measured by nLUC assay (Promega) using a SpectraMax plate reader (Molecular Devices).
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bioRxiv - Immunology 2020Quote: ... using Moloney murine leukemia virus reverse transcriptase (Promega) according to manufacturers’ instructions ...
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FACS-Sortable Picoreactors for Ultra High-throughput Screening of Catalysts in Biphasic EnvironmentsbioRxiv - Bioengineering 2024Quote: ... emulsions of octanol + 5% (w/v) Span 80 + 40 µM Nile Red in an aqueous phase of nuclease free water (Promega) + 200 ng/µL miniprepped plasmid + 5% (w/v ...
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bioRxiv - Biochemistry 2024Quote: ... was synthesised from 2 µg of total RNA using the Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT; Promega, Madison, WI, USA) using random primers and oligo-dT primers (3:1 mol ...
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bioRxiv - Cell Biology 2021Quote: ... conducted with Moloney murine leukemia virus reverse transcriptase (Promega) and random hexamers (Roche Applied Science ...
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bioRxiv - Neuroscience 2023Quote: ... and Moloney murine leukemia virus MMLV reverse transcriptase (Promega) was performed according to the vendor’s protocol.
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bioRxiv - Microbiology 2023Quote: ... Rabbit Reticulocyte Lysate (Promega), ATP regeneration system (0.5 mM ATP [Wako]) ...
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bioRxiv - Physiology 2020Quote: ... Larvae with intestinal tracts clearly outlined with Nile Red dye were embedded in 1% low melting point agarose (Promega, Madison, WI) in E3 supplemented with 0.16 mg/mL tricaine in 6-well glass bottom plates (#1.5 high performance cover glass ...
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bioRxiv - Cancer Biology 2021Quote: ... 1 mg/mL 50 μL aliquots of cleared lysates were incubated with 2 μL of indicated trypsin concentration (Promega) at 4°C for 15 min ...
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bioRxiv - Genomics 2022Quote: ... 2 µl of yeast lysate was used as a template in a 10 µl GoTaq Green reaction (Promega M7123) with 0.25 µM of primers ...
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bioRxiv - Genomics 2023Quote: ... 2 µL of yeast lysate was used as a template in a 10 µL GoTaq Green reaction (Promega M7123) with 0.25 µM of primers ...
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bioRxiv - Bioengineering 2022Quote: ... Virus growth was measured with NanoGlo Luciferase Assay System (Promega) and cell viability was measured with CellTiter-Glo 2.0 Cell Viability Assay (Promega) ...
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bioRxiv - Biochemistry 2020Quote: Rabbit reticulocyte lysates (RRL, Promega) were used to investigate the inhibition of translation by DPR proteins ...
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bioRxiv - Microbiology 2023Quote: ... Lysates were harvested 2 days hours following transfection and reporter assays were performed using the Dual-Luciferase Reporter Assay (Promega).
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bioRxiv - Biochemistry 2022Quote: ... In-vitro translations were done with rabbit reticulocyte lysate (Flexi Rabbit Reticulocyte Lysate System, Promega) and translated CFTR (domains ...
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bioRxiv - Microbiology 2019Quote: ... 200 U of Moloney murine leukemia virus reverse transcriptase (MMLV; Promega) in 30 mM Tris and 20 mM KCl (pH 8⋅3 ...
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bioRxiv - Cell Biology 2020Quote: ... Virus DNA liposomes were prepared using FuGENE HD Transfection reagent (Promega) and DMEM ...
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bioRxiv - Biochemistry 2021Quote: ... and 1 unit/μl Avian Myoblastosis virus reverse transcriptase (Promega®) were added and incubated for 1h at 30°C ...
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bioRxiv - Microbiology 2023Quote: ... Virus infectivity was measured by β-galactosidase activity (Promega, Madison, WI). Virus stocks were concentrated (20X ...
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bioRxiv - Microbiology 2023Quote: Virus neutralization was measured using a β-galactosidase-based assay (Promega) with TZM.bl target cells (85) ...
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bioRxiv - Cell Biology 2023Quote: ... and reverse-transcribed using Moloney murine leukemia virus reverse transcriptase (Promega). cDNA was amplified in a thermal cycler (GeneAmp PCR System 9700 ...
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bioRxiv - Cancer Biology 2024Quote: ... Moloney Murine Leukemia Virus Reverse Transcriptase RNase H-Point Mutant (Promega) was used for cDNA synthesis ...
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bioRxiv - Biochemistry 2022Quote: ... or uncoupled reticulocyte lysate system (Promega). For the import reaction ...
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bioRxiv - Cell Biology 2019Quote: ... and rabbit reticulocyte lysate system (Promega) respectively ...
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bioRxiv - Biochemistry 2020Quote: The Rabbit Reticulocyte Lysate System (Promega) was used to determine the influence of internal modifications on translation efficiency of RNA F ...
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bioRxiv - Biophysics 2023Quote: Rabbit reticulate lysate (RRL, L4960, Promega) is used as the artificial cytoplasm to fulfill the requirement of cytosolic factors necessary for nuclear transport (S ...
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bioRxiv - Cancer Biology 2024Quote: Rabbit reticulocyte lysates (RRL) (Promega L4960) were treated with micrococcal nuclease (NEB M0247S ...
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bioRxiv - Molecular Biology 2019Quote: ... and cDNA was generated using the Moloney Leukemia Virus Reverse Transcriptase (Promega), both according to the manufacturer’s protocol.
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bioRxiv - Developmental Biology 2019Quote: ... and reverse transcribed using Moloney murine leukaemia virus reverse transcriptase enzyme (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... virus stocks were first treated with 50 units/ml RQ1-DNase (Promega) for 1 h to remove any plasmid DNA ...
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bioRxiv - Systems Biology 2019Quote: ... the cells were immediately re-plated in 2 ml of complete medium for 24 hours and then lysates were analyzed with the Dual Luciferase reporter system (Promega Catalog No. E1910).
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bioRxiv - Systems Biology 2020Quote: ... 5 µg of K562 cell lysate (Promega) or 5 µg yeast digests were injected and run on a nanoAcquity UPLC (Waters ...
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bioRxiv - Microbiology 2021Quote: ... a rabbit reticulocyte lysate (Promega, Cat: L4151) was thawed at 37°C immediately before use and supplemented with 40 μl of 1 mM hemin stock solution and 10 μl of 1 M creatine phosphate (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2022Quote: ... Rabbit reticulocyte lysates were obtained from Promega. For each experimental replicate ...
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bioRxiv - Microbiology 2020Quote: ... Lysates were combined with Luciferase Substrate (Promega) and luminescence was measured with a FLUOstar Omega plate reader (BMG Labtech) ...
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bioRxiv - Molecular Biology 2019Quote: ... and reverse transcribed with Moloney murine leukemia virus reverse transcriptase RNase H(−) (Promega) using oligoT primer ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Each RNA sample was reverse transcribed using murine leukemia virus reverse transcriptase (Promega) and random DNA hexamer primers ...