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Citations for Promega :
1 - 50 of 636 citations for Vertical Gel Casting Cassettes since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... the HaloTag cassette from pHTC HaloTag CMV-neo Vector (Promega; G7711), was PCR-amplified and appended to the carboxy-terminus of synaptophysin (SYP ...
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bioRxiv - Genomics 2019Quote: ... a minimal promoter and luciferase cassette derived from pNL3.2 (Promega, Madison, WI) was inserted in between the genomic sequences and the barcode using KpnI and XbaI sites ...
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bioRxiv - Cancer Biology 2024Quote: Firefly luciferase expression cassette (fluc+) was excised from pGL4.50 vector (Promega, # E1310) using NdeI and BamHI restriction enzymes and cloned into pLenti6NEO vector using the same restriction sites ...
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bioRxiv - Plant Biology 2021Quote: Full length Luciferase coding sequence was PCR amplified from the pGEM-luc cassette vector (Promega) using the primer pair ...
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bioRxiv - Neuroscience 2021Quote: ... the HaloTag cassette was amplified from the pHTC HaloTag® CMV-neo Vector (Promega; G7711) and amended to either the amino or carboxy terminus of SYT7a constructs ...
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bioRxiv - Molecular Biology 2023Quote: ... Sweden) was engineered by inserting the PGK-NLuc-poly(A) cassette from pNL1.1.PGK (Promega) plasmid into virus genome ...
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bioRxiv - Cell Biology 2023Quote: ... A chimeric gene cassette consisting of a GPI-anchored YFP fused to HaloTag® (Promega) followed by the reverse tTA (rtTA ...
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bioRxiv - Cell Biology 2019Quote: ... The MultiSite Gateway cassette was then ligated into pGEM-T Easy Vector (Promega UK, Southampton, UK) and the insert sequence was confirmed by sequencing (Source BioScience ...
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bioRxiv - Genomics 2023Quote: ... we replaced the Luc2-IRES-ZsGreen reporter with a cassette encoding H2B fused to Nanoluciferase (Promega) to minimize background luminescence ...
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bioRxiv - Molecular Biology 2020Quote: ... plasmid pGL4.32 containing a firefly luciferase cassette under NF-κB response elements was obtained from Promega (Cat# E8491). For overexpression of ubiquitin ...
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bioRxiv - Cell Biology 2019Quote: ... was amplified from mouse genomic DNA and cloned upstream of luciferase cassette in KpnI/HindIII site of pGL4.23 (Promega) using primers listed in Supplementary table 1 ...
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bioRxiv - Cell Biology 2019Quote: ... parental HeLa cells were transfected with 2 μg plasmid containing a GFP cassette and 4 μl FuGENE HD (Promega) in a six-well cluster ...
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bioRxiv - Bioengineering 2022Quote: ... while the complementary sequence was cloned downstream of the Renilla luciferase reporter cassette in a psiCHECK-2 plasmid (Promega). The same target sequence was also cloned downstream of a TagRFP reporter cassette in an ePB-BSD-TT piggyback vector (see plasmids ...
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bioRxiv - Evolutionary Biology 2021Quote: ... gel-purified (Wizard SV Gel and PCR Clean-Up System, Promega) and blunted using PfuUltra HF DNA polymerase (Agilent Technologies) ...
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bioRxiv - Biophysics 2023Quote: ... Then the gel was dried using a gel drying kit (Promega) and exposed to a phosphor screen (overnight) ...
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bioRxiv - Cell Biology 2024Quote: ... Gel pieces were destained and in-gel trypsin digested (Promega, V5280). The peptide mixture was purified on PepClean C18 spin columns (Thermo Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... excised gel segments were subjected to in-gel trypsin (Promega, V5111) digestion and dried ...
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Redundant and specific roles of cohesin STAG subunits in chromatin looping and transcription controlbioRxiv - Cell Biology 2019Quote: ... the gRNA/Cas9 plasmid as well as the plasmid carrying homology arms and mAID-mClover neomycin cassette were transfected into HCT116 CMV-OsTIR1 cells using FuGene HD (Promega). After 6 days selection with 700 µg/ml G418 the cells were FACS-sorted for mClover expression and seeded into 96 well plates to grow single clones ...
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bioRxiv - Plant Biology 2022Quote: ... Each of the target site sequences was introduced into Arabidopsis U6 promoter-gRNA cassette cloned into pGEM-T Easy plasmid (Promega) using two-step overlapping PCR (Figure S1) ...
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bioRxiv - Molecular Biology 2023Quote: ... Flp-In T-REX HeLa cells were transfected with 1.8 μg of pOGG4 plasmid and 200 ng of pcDNA5/FRT/TO plasmid encoding either a GFP-BICD2N-MTS or a GFP-MTS cassette using FuGENE HD (Promega). Cells with a stably integrated transgene were selected with 150 μg/mL hygromycin over 4-5 days ...
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bioRxiv - Microbiology 2024Quote: ... Flp-In T-REX HeLa cells were transfected with 1.8 μg of pOGG4 plasmid and 200 ng of pcDNA5/FRT/TO plasmid encoding the relevant transgene cassette using FuGENE HD transfection reagent (Promega). Cells with a stably integrated transgene were selected with 150 μg/mL hygromycin for 4-5 days ...
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bioRxiv - Molecular Biology 2022Quote: ... Each lane was sliced into nine gel bands and the excised gel pieces were subjected to in-gel digestion using trypsin (Promega) followed by C18 STAGE tip clean-up (as described in 4).
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bioRxiv - Molecular Biology 2021Quote: ... Following gel extraction using Wizard SV Gel and PCR CleanUp System (Promega), 500 ng of purified and linearized vector and 200 ng of each specific gene were treated with T4 DNA polymerase enzyme (Fermentas ...
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bioRxiv - Evolutionary Biology 2021Quote: ... then gel extracted (Promega Wizard SV Gel and PCR Clean-Up System). Linear plasmids were subjected to bisulfite conversion using the EpiMark Bisulfite Conversion Kit (New England Biolabs) ...
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bioRxiv - Cell Biology 2022Quote: ... and gel slices were subjected to in-gel digestion with Trypsin (Promega) at 37°C overnight ...
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bioRxiv - Neuroscience 2023Quote: ... Individual gel piece was subjected to in-gel digestion using trypsin (Promega). The resulting dried peptides were analyzed in technical duplicate on a ThermoFisher Orbitrap Fusion Lumos Tribrid mass spectrometry system equipped with an EASY-nLC 1200 ultrahigh-pressure liquid chromatography system interfaced via a Nanospray Flex nanoelectrospray source ...
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bioRxiv - Microbiology 2023Quote: ... gel using the Wizzard SV Gel and PCR clean system (Promega, USA) and quantified with Qubit dsDNA HS assay (Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2023Quote: ... Subsequently we ran the digested fragment on a 1% TAE agarose gel and the desired ∼14 kbp DNA fragment was isolated from an agarose gel using a gel purification kit (Promega, A9282).
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bioRxiv - Biophysics 2023Quote: ... from the access handles by running this on a 1% agarose gel and gel purify the right DNA fragment with a gel purification kit (Promega, A9282).
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bioRxiv - Genomics 2019Quote: ... and gel extraction (Wizard™ SV Gel and PCR Cleanup System [Promega A9282]) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... gel pieces were subjected to in-gel digestion with sequencing-grade trypsin (Promega), and the extracted peptides were resolved by reverse phase HPLC ...
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bioRxiv - Molecular Biology 2022Quote: ... PCR products were purified by agarose gel electrophoresis and extracted from the gel using the Wizard SV Gel and PCR Clean-Up System (Promega, Madison, WI, USA). Purified PCR products were cloned into a pCR2.1-TOPO vector (Invitrogen ...
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bioRxiv - Microbiology 2023Quote: ... The PCR product was run on a 2% agarose gel using gel electrophoresis and the band was purified with the Wizard® SV Gel and PCR Clean-Up System (Promega, Madison, USA). Samples were submitted to Macrogen Europe (Amsterdam ...
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bioRxiv - Microbiology 2021Quote: ... and gel bands excised before in-gel digestion using modified trypsin (Promega, sequencing grade). Resulting peptides were analyzed by online nanoliquid chromatography coupled to tandem MS (UltiMate 3000 RSLCnano and Q-Exactive HF ...
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bioRxiv - Cell Biology 2022Quote: Gel bands were subjected to in-gel digestion with sequencing-grade modified trypsin (Promega) as detailed in Belluzzi et al. ...
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bioRxiv - Genomics 2022Quote: ... products were gel purified using the Wizard SV Gel and PCR Cleanup kit (Promega) and ligated into pDONR221-b1b4 (Invitrogen ...
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bioRxiv - Microbiology 2024Quote: ... Amplicons were subsequently gel-extracted (Wizard SV Gel and PCR Clean-Up System, Promega), quantified ...
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bioRxiv - Cell Biology 2022Quote: ... The gel slices were reduced and in-gel digested with sequencing grade modified trypsin (Promega). The resulting samples were quenched by 10% trifluoroacetic acid and peptides were extracted and dissolved in 0.1% trifluoroacetic acid ...
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bioRxiv - Microbiology 2022Quote: ... gel purified using a Wizard® SV gel and PCR clean up system (Promega, Australia) and sequenced using an Illumina MiSeq platform at the Ramaciotti Centre for Genomics ...
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bioRxiv - Microbiology 2024Quote: ... Amplicons were gel-purified using Wizard® SV Gel and PCR Clean-Up System (Promega) and quantified with a Qubit 4 Fluorometer (Thermofisher scientific ...
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bioRxiv - Cell Biology 2019Quote: An attR3/attR4 MultiSite Gateway cassette was PCR-amplified from pK7m34GW (Karimi et al., 2005) using GoTaq DNA Polymerase (Promega UK, Southampton, UK) with primers NdeI-R3 and NdeI-R4 (Table S2) ...
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bioRxiv - Microbiology 2019Quote: ... PCR products were concentrated and gel purified using Wizard SV Gel and PCR cleanup protocol (Promega). Binding reactions consisted of the manufacturer’s binding buffer ...
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bioRxiv - Cancer Biology 2020Quote: ... PCR products were gel-purified using the Wizard SV Gel and PCR Clean-Up System (Promega) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: ... Gel bands for heavy and light chains were excised and in-gel digested using trypsin (Promega), chymotrypsin (Promega) ...
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bioRxiv - Plant Biology 2022Quote: ... The PCR fragment was gel-purified using Wizard SV gel and PCR Clean-up System (Promega), ligated into pGEM-T (Promega ...
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bioRxiv - Plant Biology 2023Quote: ... DNA fragments were excised from the gel by means of x-tracta™ Gel Extractor (Promega) and purified using Wizard® SV Gel and PCR Clean-Up System (Promega) ...
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bioRxiv - Cell Biology 2023Quote: ... in-gel digestion with trypsin (Promega) overnight ...
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bioRxiv - Neuroscience 2021Quote: ... was purified using gel clean-up kit (Wizard® SV Gel and PCR Clean-Up, A9281, Promega) (Figure S4B) ...
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bioRxiv - Plant Biology 2021Quote: ... The entire protein region of the gel was excised and subjected to in-gel trypsin (Promega V511A) digestion after reduction with DTT and alkylation with iodoacetamide ...
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bioRxiv - Genetics 2020Quote: ... purified from agarose gels using the Wizard SV Gel and PCR Clean-Up System (Promega, Madison, USA) and sent for sequencing (to GATC Services-Eurofin Genomics ...