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Citations for Promega :
1 - 50 of 4441 citations for Triiodothyronine T3 ELISA Kit 5 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: ... using the T7/T3 Riboprobe Combination System kit (Promega) and the Digoxigenin labeling kit (Roche) ...
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bioRxiv - Developmental Biology 2021Quote: ... T7 or T3 (Promega) and DIG-labelled nucleotides ...
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bioRxiv - Biophysics 2023Quote: ... ELISA plates were read on a GloMax plate reader (Promega, Wisconsin, US). Data were converted from concentration to total mass based on the volume of media/pellet ...
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bioRxiv - Neuroscience 2023Quote: ... or T3 RNA polymerases (Promega) and purified through Micro Bio-Spin Columns with Bio-Gel (BIO-RAD) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... and T3 RNA polymerase (Promega, P2083).
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bioRxiv - Molecular Biology 2020Quote: Strand-specific RNA probes were generated using a T3 in vitro transcription kit (Promega) in the presence of Chromatide AlexaFluor-UTP (ThermoFisher) ...
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bioRxiv - Neuroscience 2020Quote: ... then transcribed with T3 RNA polymerase (Promega) in the presence of Ribo m7G Cap Analog (promega ...
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bioRxiv - Biochemistry 2022Quote: RNAs were synthesized with T3 RNA polymerase (Promega) in the presence of [α-32P]UTP and 7 mM “anti-reverse” cap analog (m7,3’-OGpppG ...
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bioRxiv - Developmental Biology 2019Quote: ... we added 2µL of T3 RNA Polymerase (Promega) to each sample as well as after 30min at 37°C for another 1h30 ...
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bioRxiv - Neuroscience 2020Quote: ... The probe was synthesized using T3 RNA polymerase (Promega) and labeled with DIG RNA label mix (Roche) ...
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bioRxiv - Developmental Biology 2023Quote: ... using either the T3 or SP6 riboprobe system (Promega) with modifications from the manufacturer’s suggested protocol ...
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bioRxiv - Neuroscience 2019Quote: ... Antisense probes were synthesized with T3 RNA polymerase (Promega #2083) and labeled with alpha-35S-CTP (PerkinElmer #NEG064H250UC ...
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bioRxiv - Immunology 2020Quote: ... Total IgG and ANA ELISA plates were read using a GlowMax Discover Microplate Reader (Promega) on the absorbance setting at 450nm.
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bioRxiv - Immunology 2022Quote: ... ELISA plates were immediately read using a GloMax®-Multi Detection System microplate reader (Promega) at 450nm absorbance.
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bioRxiv - Cancer Biology 2021Quote: ... and plates were incubated for 5 days before being assayed using the Cell Titer-Glo kit (Promega).
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bioRxiv - Physiology 2019Quote: In vitro transcription was performed using T3 RNA polymerase and other reagents supplied in the RiboProbe In Vitro Transcription System kit (Promega, Italy) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... ELISA plates were immediately read using a GloMax®-Multi Detection System microplate reader (Promega, Madison WI) at 450 nm absorbance.
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bioRxiv - Immunology 2023Quote: ... Insulin and NEFA levels were using a mouse insulin ELISA kit (Promega) and WAKO NEFA-C kit (WAKO ...
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bioRxiv - Physiology 2023Quote: ... GLP-1 was detected by indirect sandwich amide chemiluminescence ELISA using a luminescence plate reader (GloMax Promega, USA). This total GLP-1 assay detects GLP-1 (7-36 ...
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bioRxiv - Neuroscience 2019Quote: ... Anti-sense RNA was then in vitro transcribed using a T3 RNA polymerase (Promega P2083) and a DIG RNA labeling mix (Roche 11277073910 ...
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bioRxiv - Genetics 2021Quote: ... DIG-labelled RNA probes were produced by in vitro transcription with T3-RNA polymerase (Promega). The Crabp1 probe template was amplified from a plasmid containing Crabp1 cDNA sequence ...
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bioRxiv - Neuroscience 2020Quote: ... The linearized plasmids were used as templates for in vitro transcription with T3 RNA polymerase (Promega) according to the manufacturer’s instructions.
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bioRxiv - Neuroscience 2023Quote: ... The linearized plasmids were used as templates for in vitro transcription with T3 RNA polymerase (Promega) according to the manufacturer’s instructions.
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bioRxiv - Developmental Biology 2019Quote: Digoxigenin (DIG) and fluorescein (FLU)-labelled RNA probes were synthesized using T7 or T3 RNA polymerases (Promega) according to manufacturers’ instructions and supplied with DIG or FLU labelled UTP (Roche) ...
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bioRxiv - Cell Biology 2021Quote: ... the dsRNAs were synthesized from the products using T3 and T7 RNA polymerases (Promega, P2075, and P2083). The transcription products were incubated at 70 °C for 10 min and at 37 °C for 30 min for annealing ...
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bioRxiv - Neuroscience 2020Quote: ... The antisense robo2 probe was synthesized from pBlueScript-robo2 linearized with EcoRI using T3 RNA polymerase (Promega); the sense robo2 probe was synthesized from pBlueScript-robo2 linearized with XhoI using T7 RNA polymerase (Promega) ...
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bioRxiv - Molecular Biology 2019Quote: 1875 ng of circular plasmids were incubated at 37°C with 50 U of T3 RNA Polymerase (Promega) in a transcription buffer (40 mM Tris-HCl pH 7.9 ...
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bioRxiv - Neuroscience 2022Quote: ... for the synthesis of sense and antisense single-stranded RNAs (ssRNA) with T7 and T3 RNA polymerase (Promega), respectively ...
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bioRxiv - Neuroscience 2023Quote: ... transferred to opaque 96-well plates containing 5 μM furimazine (NanoLuc Luciferase Assay, Promega), and bathed in 5-HT at various concentrations (ranging from 0.1 nM to 1 mM) ...
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bioRxiv - Cell Biology 2023Quote: ... A direct cAMP ELISA kit (Enzo) was used in endpoint experiments and the pGloSensor (−20F) luminescence assay (Promega) was used for kinetic cAMP measurements ...
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bioRxiv - Cell Biology 2019Quote: ... cDNA sequences in pCMV-3Tag1A backbones were transcribed and translated in vitro using the TNT T3 coupled reticulocyte system (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2020Quote: ... The DNA templates used for DIG-labeled STX18-AS1 RNA probe in vitro syntheses (Supplementary file) with T3 RNA polymerase (Promega) were amplified from the coding sequence of STX18-AS1 from human foetal hearts cDNA (obtained from HDBR) ...
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bioRxiv - Developmental Biology 2021Quote: ... using primers 1 and 2 (Supplementary Table) and used for making a Digoxigenin (DIG)-labeled FISH probe using T3 RNA polymerase (P2083, Promega). After T3 RNA polymerase reaction at 37°C for 4 hours ...
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bioRxiv - Developmental Biology 2021Quote: ... DIG-labeled RNA probes were produced using DIG-UTP by in vitro transcription with T3 RNA polymerase (Promega, Catalog No. P208C) according to the Roche Applied Science’s protocol (Roche Applied Science ...
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bioRxiv - Neuroscience 2023Quote: ... Antisense probe was generated by cleavage of pSC-dmist plasmid with XbaI and in vitro transcribed with T3 polymerase (Promega P2083) using 1 µg DNA template according to the standard in vitro transcription protocol (see the full protocol at dx.doi.org/10.17504/protocols.io.ba4pigvn) ...
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bioRxiv - Developmental Biology 2024Quote: ... The katna1 digoxigenin-labelled sense and antisense riboprobes were synthetized from the linearised recombinant TOPO® TA cloning vector using T7 and T3 RNA Polymerase (Promega) according to the supplier’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... Baseline fluorescence was measured in an ELISA reader (Promega) following an incubation of 30 min at 37°C ...
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bioRxiv - Bioengineering 2023Quote: ... 50 µL of the cell and 1xPBS mixture were transferred into a 96-well plate bottom white plate (Costar Cat#3922) and lysis buffer from Nano-Glo Luciferase Assay Kit (Promega Cat#N1120) was added directly to the plates following the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2020Quote: ... After the addition of 5 mM ATP the reaction was taken to a plate reader (Promega GloMax Navigator) and the luminescence collected for approximately 30 minutes ...
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bioRxiv - Cancer Biology 2020Quote: ... Plates were incubated for 72hr at 37°C with 5% CO2 and then measured by CellTiter-Glo assay (Promega). Briefly ...
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bioRxiv - Immunology 2023Quote: ... plates were washed 5 times with wash buffer (PBS with 1% BSA (Capricorn Scientific) and 0.05% Tween-20 (Promega)) ...
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bioRxiv - Molecular Biology 2023Quote: ... ATRX 5’-TGAAACTTCATTTTCAACCAAATGCTC-3’ and 5’-ATCAAGGGGATGGCAGCAG-3’ All PCR reactions were performed using GoTaq® G2 DNA polymerase kit from Promega following the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2022Quote: ... and 72h in 3-5 wells for each condition (mean values of 3-5 technical replicates are provided for each donor) using a multiwell plate reader Glomax (Promega). Each 2h incubation was performed at a multiplicity of infection (MOI ...
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bioRxiv - Microbiology 2022Quote: ... was added to the plate and incubated at room temperature for 5 min before measuring luminescence on a GloMax 96 luminometer (Promega).
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bioRxiv - Cell Biology 2022Quote: ... 5×104 cells/well were seeded in 24-well plates and transfected with 10 ng pRL-CMV (Promega, Walldorf, Germany) together with either 250 ng pSuper8xTOPFlash or 250 ng pSuper8xFOPFlash [22] using the FuGENE6® reagent (Promega) ...
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bioRxiv - Cancer Biology 2019Quote: ... Luciferase and Renilla activities were measured by Biotek Synergy 2 plate reader (Winooski, VT) using the Dual-luciferase assay kit (Promega, Madison, WI).
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bioRxiv - Cancer Biology 2020Quote: ... the full-length fragment of human PFKFB3-5 was amplified with primers PFKFB3-5 reverse and PFKFB3-5 forward and subcloned into the plasmid pGEM-T using the T/A Cloning Kit (Promega, Mannheim, Germany). Subsequently ...
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bioRxiv - Bioengineering 2023Quote: ... The effect on cell viability of PTNP was assessed using the MTS [(3-(4,5-dimethylthiazol-2-yl)-5-(3- carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium)] assay (CellTiter 96 cell proliferation assay kit; Promega, WI, USA) (92) ...
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bioRxiv - Microbiology 2021Quote: ... Bright-Glo reagent was added to the plate and incubated at room temperature for 5 min before measuring luminescence on a GloMax 96 luminometer (Promega, USA), with titers given in Relative Light Units (RLU ...
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bioRxiv - Synthetic Biology 2022Quote: To measure luciferase activity on plaque containing LB-agar plates, 5 μl of the diluted (1:50, in PBS) NanoLuc® luciferase substrate 2-furyl methyl-deoxy-coelenterazine (Furimazine; Promega) were dropped onto the respective plaques ...