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Citations for Promega :
1 - 50 of 1088 citations for Toll Like Receptor 5 TLR5 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... Trypsin-Like and Caspase-Like Cell-Based Assays (Promega), according to manufacturer’s instructions (Moravec et al. ...
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bioRxiv - Cell Biology 2024Quote: ... Trypsin-Like and Caspase-Like Cell-Based Assay (Promega) and GloMax® Discover microplate reader (Promega ...
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bioRxiv - Cell Biology 2021Quote: ... Proteasome activities (chymotrypsin-like, trypsin-like and caspase-like) were assayed using the Proteasome-Glo kit (Promega, USA) in 384-well plates for luminescent assays ...
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bioRxiv - Cell Biology 2023Quote: Cell-based ProteasomeGlo™ chymotrypsin-like and trypsin-like assays (Promega) were performed according to manufacturer’s instructions (Moravec et al ...
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bioRxiv - Cell Biology 2022Quote: Three major proteasome activities (chymotrypsin-like trypsin-like, caspase-like) were measured using the Proteasome-Glo Cell-Based Assay Kit (G1180, Promega) as described by the manufacturer ...
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bioRxiv - Cell Biology 2021Quote: ... trypsin-like and caspase-like activities were measured separately using the relevant substrates from Promega (Suc-LLVY-Glo ...
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bioRxiv - Neuroscience 2021Quote: Proteasome-Glo Chymotrypsin-like assay (Promega G8621) was used for proteasome assays ...
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bioRxiv - Developmental Biology 2021Quote: The Promega Proteasome-Glo Chemotrypsin-like assay (Promega cat. no. G8660) was used following the manufacturer’s protocols ...
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bioRxiv - Cancer Biology 2023Quote: Proteasome activity was estimated using Proteasome Glo® Chemotrypsin-like kit (Promega) following manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: Surface expression was measured using a HiBit-tagged 5-HT2A receptor and the Nano-Glo HiBit Extracellular Detection System (Promega). N-terminal HiBit-tagged human 5-HT2A receptor was cloned into pcDNA3.1 using Gibson Assembly ...
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bioRxiv - Biochemistry 2022Quote: ... and M75F receptor plasmids using FuGene HD (Promega). Cells were treated with DMSO or varying drug concentrations 24 hours after transfection ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Proteasome-Glo™ chymotrypsin-like cell-based assay reagents were added according to manufacturer instructions (Promega). Luminescence was read using a CLARIOstar® microplate reader (BMG Labtech).
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bioRxiv - Neuroscience 2023Quote: The proteasome activity assay was performed using a Proteasome-Glo Chymotrypsin-Like Cell-Based Assay kit (Promega). Briefly ...
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bioRxiv - Developmental Biology 2019Quote: Assays for proteasome activity were performed using the Proteasome-Glo™ Chymotrypsin-Like Cell-Based Assay Kit (Promega) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: Manufacturer protocols were followed for the proteasome activity assay (Proteasome-Glo Chymotrypsin-like cell-based assay, Promega, G8660). The fluorescence was measured on the Promega GloMax plate reader.
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bioRxiv - Cancer Biology 2024Quote: ... senescent and diapause-like SK-Mel-103 and A549 cells using CellTiter-Glo Luminescent Cell Viability Assay (Promega, #G7571) following manufacturer’s instructions or Crystal Violet (Merck ...
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bioRxiv - Biochemistry 2023Quote: ... cells were transfected with 1.5 μg receptor and 1 μg GloSensor-22F (Promega). After 24 hours ...
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bioRxiv - Cell Biology 2020Quote: ... 10 g of the muscle lysates were used for determining proteasome-associated chymotrypsin-like activity with the Proteasome-GloTM Assay Systems (#G8531, Promega). Luminescence signals were detected with the SpectraMax i3x Multi-Mode Microplate Reader (Molecular Devices ...
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bioRxiv - Cell Biology 2021Quote: Proteasome activity was measured in iPSC-CMs using the Proteasome-Glo™ Chymotrypsin-Like Assay according to manufacturer’s instructions (Promega). Cells were incubated with the inhibitors (ranging from 2 to 5000 nM ...
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bioRxiv - Molecular Biology 2019Quote: ... Virus-like particles (VLPs) containing the GFP control or targeting sgRNAs were generated by transfection of HEK293 cells with FuGene (Promega). VLPs were collected from culture supernatant ...
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bioRxiv - Evolutionary Biology 2023Quote: Coding sequences of Nodal and Gdf1/3-like were amplified from cDNA templates of amphioxus embryos and ligated into the pXT7 vector using T4 DNA ligase (Promega). Due to low expression level ...
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bioRxiv - Molecular Biology 2023Quote: ... was removed by evaporation using speed-vac and the residue was used to produce L-[2,3,4,5-3H]-proline labeled procollagen chains and the collagen-like proteins in the TnT® Coupled Reticulocyte Lysate Systems (Promega) using either SP6 or T7 promoters ...
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bioRxiv - Microbiology 2023Quote: ... a pPolI-Firefly plasmid encoding a negative-sense viral-like RNA expressing the Firefly luciferase and the pTK-Renilla plasmid (Promega) as an internal control ...
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bioRxiv - Molecular Biology 2023Quote: ... a pPolI-Firefly plasmid encoding a negative-sense viral-like RNA expressing the Firefly luciferase and the pTK-Renilla plasmid (Promega) as an internal control ...
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bioRxiv - Microbiology 2020Quote: The increase in caspase like enzymatic activity was evaluated by using a staining solution containing (FITC)-VAD-FMK (CaspACE™, Promega) at a final concentration of 10 µM/ml (74) ...
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bioRxiv - Cancer Biology 2020Quote: ... the blot was blocked with either 5% nonfat-milk or 5% BSA in TBST before addition of primary antibodies and followed with peroxidase-conjugated secondary antibody (Promega). Protein bands were detected using SuperSignal Chemiluminescent Substrate (Pierce ...
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bioRxiv - Biochemistry 2022Quote: HEK293 T cells were co-transfected with the target receptor construct and GloSensor cAMP reporter (Promega) in DMEM supplemented with 10% FBS for overnight incubation ...
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bioRxiv - Biochemistry 2024Quote: lrl 1μg of N-terminally FLAG-tagged receptor and 1μg of F22 (Promega, Cat. no: E2301) (for GloSensor assay)
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bioRxiv - Immunology 2024Quote: ... 3×106 ADCC and ADCP Bioassay Effector Cells expressing the human FcγRIIIa or FcγRIIa receptors (Promega) were added per well and the cells were incubated in at 37°C with 5% CO2 for 6 hours ...
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bioRxiv - Molecular Biology 2021Quote: ... was digested with the same enzymes and ligated with the amplified receptors using T4 DNA ligase (Promega).
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bioRxiv - Biochemistry 2021Quote: ... HEK-293 cells were transfected with FLAG-tagged receptor (3.5 μg) and luciferase-based 22F cAMP biosensor construct (3.5 μg) (Promega). 14–16 h post transfection ...
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bioRxiv - Cell Biology 2024Quote: ... HaloTag-receptor expressing mESCs were fluorescently labeled with either 1 µM of HaloTag AlexaFluor 488 ligand (Promega, G1001) or 3.5µM of HaloTag AlexaFluor 660 ligand (Promega ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: To measure ATP bound to detergent purified P2X1 receptor a Kinase-Glo® Luminescent Kinase Assay (ProMega, USA) was used ...
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bioRxiv - Microbiology 2021Quote: ... 5 μl 5× Buffer (Promega), 2 μl MgCl2 (25mM) ...
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bioRxiv - Neuroscience 2020Quote: ... The pCAG-DCC:TDTOMATO wildtype and missense mutant receptor constructs (1.764 µg) were also transfected into HEK293T cells cultured on acid-washed coverslips using FuGENE HD (Promega) in Optimem (Gibco ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were transfected with 2 μg of FLAG-tagged β2 or β1 adrenergic receptor DNA using Fugene 6 (Promega) and seeded at a density of ∼70,000 cells.ml−1 on 18 mm glass coverslips 4-8 hours after transfection.
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bioRxiv - Biophysics 2023Quote: ... HEK293T cells were co-transfected with FLAG-tagged receptor (3.5μg) and luciferase-based 22F cAMP biosensor construct (3.5μg) (Promega, Cat. no: E2301) using polyethyleneimine (PEI ...
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bioRxiv - Biophysics 2021Quote: ... sapiens TACAN-eGFP or the M2 muscarinic receptor (Halo-M2R-eGFP) plasmid (previously established in this lab) was transfected into the cells using FugeneHD (Promega) following the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2022Quote: ... HEK293 cells were co-transfected with FLAG-tagged receptor constructs and luciferase-based 22F cAMP biosensor (Promega, Cat. no. E2301) using polyethylenimine (PEI ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... were co-transfected with pcDNA5/FRT construct encoding haemagglutinin (HA)-tagged human CB1 receptor cDNA and pOG44 (Flp recombinase plasmid) using transfection reagent Fugene HD (Promega) as previously described for AtT-20 pituitary tumour cells [26] ...
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bioRxiv - Cancer Biology 2023Quote: ... PCR was used to amplify the coding regions of the steroid receptor genes and to linearize the expression plasmids pHTN HaloTag CMV Neo or pFLN-1 NanoLuc (Promega #N1811 ...
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bioRxiv - Cell Biology 2023Quote: Protein-protein interactions between Mkl1/2 and Foxo1/3/4 or glucocorticoid receptors were also measured using NanoBiT PPI Starter Systems (Promega). 293FT cells were seeded in 96-well white wall microplates and co-transfected with the LgBiT and SmBiT plasmids ...
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bioRxiv - Biochemistry 2024Quote: ... GRK371 and β-arrestin272 recruitment to chemokine receptors (CXCR1, CXCR2, CXCR3-A, CXCR4, CXCR5, CXCR6 and ACKR3) was monitored using a nanoluciferase complementation-based assay (NanoBiT, Promega)73,74 ...
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bioRxiv - Biochemistry 2024Quote: ... HEK293T cells were transfected with 3.5μg of N-terminally FLAG-tagged receptor and 3.5μg of an SRE-based luciferase reporter plasmid pGL4.33 (Promega, Cat. no: E1340). 14-16h after transfection ...
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bioRxiv - Microbiology 2019Quote: ... 5 μl of 5× Gotaq buffer (Promega M792A), 1μl of 10μM forward primer ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... HEK293T cells were co-transfected in a 1:1 ratio with either human D1 or D2Long receptor and a split-luciferase based cAMP biosensor (GloSensor, Promega, Durham NC). The next day ...
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bioRxiv - Molecular Biology 2023Quote: ... These cells were transfected in 70% confluent 6-wells TC-treated plates with 2 µg TGFβ receptor-Nanoluciferase constructs (constructs were provided by Promega and Promega R&D) using a 1 µg DNA ...
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bioRxiv - Microbiology 2020Quote: ... The plates were washed with PBS-T 5 times and a 1:5000 dilution of HRP-conjugated goat anti-rabbit IgG antibody (Promega) was added for 45 min at 37 □ ...
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bioRxiv - Developmental Biology 2023Quote: ... The following day membranes were washed 3 times n TBTS for 5 minutes and incubated in secondary antibody (1:2500 anti-Rabbit HRP conjugated, Promega) diluted in blocking buffer for 1 hour at room temperature and washed 3 times with TBTS for 5 minutes at room temperature ...
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bioRxiv - Immunology 2020Quote: ... ExpiCHO cells then were incubated with Jurkat cells expressing FcγRIIIa receptor or FcγRIIa on their surface and stably transfected with NFAT-driven luciferase gene (Promega, Cat. Nr.: G9798 and G7018) at an effector to target ratio of 6:1 for FcγRIIIa and 5:1 for FcγRIIa ...