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Citations for Promega :
1 - 50 of 1106 citations for T 25 Flasks since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... Low passage MCF10A cells were transfected at 80% confluency in a 25 cm2 flask using 25 μl FugeneHD (Promega) with 6 μg donor plasmid (pAS4885) ...
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bioRxiv - Molecular Biology 2021Quote: ... Low passage MCF10A cells were transfected at 80% confluency in a 25cm2 flask using 25 μl FugeneHD (Promega) with 6 μg of pAS4863 and 200 ng of GBlock donor template ...
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bioRxiv - Microbiology 2020Quote: SARS-CoV-2 VLPs were produced by transfecting 2×106 293T-wt cells in T75 flasks with 25 μg total DNA using Fugene6 (Promega). The codon optimised plasmids expressing SARS-CoV-2 M ...
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bioRxiv - Molecular Biology 2019Quote: ... were plated in T25 flasks and transiently transfected with 2.5 µg plasmid DNA per flask using FuGENE HD transfection reagent (Promega) according to the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2019Quote: ... or into the pGEM-T or pGEM-T Easy vector (Promega) and subsequently sequenced (Macrogen).
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bioRxiv - Developmental Biology 2023Quote: ... or pGEM-T (Promega) vectors and eight positive colonies/gene transferred to 750 ul of ddH2O and heated to 90 C to liberate the plasmids ...
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bioRxiv - Cancer Biology 2021Quote: T Cell Activation Bioassay (Promega) was performed according to the manufacturer’s instructions ...
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bioRxiv - Pathology 2022Quote: ... The pGEM T-easy (Promega) based positive control plasmid of NNV was constructed from a 605 bp RNA2 fragment amplified from NNV-infected orange-spotted grouper (laboratory sample) ...
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bioRxiv - Microbiology 2019Quote: ... and pGEM-T Easy (Promega) were used ...
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bioRxiv - Microbiology 2022Quote: ... pGEM-T Easy vector (Promega) (supplementary data table 6) ...
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bioRxiv - Developmental Biology 2023Quote: ... cloned into pGEM-T (Promega) and used to synthesize digoxigenin-labeled probes by in vitro transcription using SP6 or T7 polymerases (Thermo-Fisher) ...
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bioRxiv - Molecular Biology 2022Quote: ... 25 mM MgCl2 (Promega), 10 μM of primers P7/P8 and either 50 μM oxo-dGTP or 1 μM dPTP ...
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bioRxiv - Evolutionary Biology 2023Quote: ... 25 mM MgCl2 (Promega), 10 µM P7/P8 and either 50 µM oxo- dGTP or 1 µM dPTP (Jena Bioscience ...
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bioRxiv - Genomics 2019Quote: ... which were subsequently ligated to pGEM-T Vector (pGEM-T Vector System I, Promega, USA), and transformed to DH5α competent cells (Z-Competent E ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... or a pGEM®-T Easy Vector (pGEM®-T Easy Vector Systems Kit, Promega, USA) depending on the Taq-polymerase used for PCR (blunt vs ...
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bioRxiv - Molecular Biology 2023Quote: ... and resulting PCR products were subcloned into a pGEM-T vector (Promega, pGEM-T Vector Systems) for subsequent Sanger sequencing of both alleles ...
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bioRxiv - Plant Biology 2019Quote: ... pGEM®-T kit from Promega were used ...
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bioRxiv - Immunology 2021Quote: ... or pGEM-T plasmid (Promega, UK), manufacturer’s instructions were followed ...
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bioRxiv - Developmental Biology 2021Quote: ... serial dilutions of pGEM-T (Promega) plasmids containing coding sequences of the target genes or internal control genes (rp49 for Drosophila melanogaster and AeRpL32 (AAEL003396 ...
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bioRxiv - Plant Biology 2020Quote: ... cloned in pGEM-t Easy (Promega), re-amplified by PCR with primers with attB tails and BP-cloned into pDONR207 (Invitrogen) ...
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bioRxiv - Neuroscience 2020Quote: The pGEM-T donor vector (Promega) was engineered by introducing a donor construct ...
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bioRxiv - Molecular Biology 2020Quote: ... subcloned via pGEM-T easy (Promega) and inserted upstream of a chloramphenicol resistance cassette in the plasmid pSF36 (pUC19+cm-FRT-HindIII ...
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bioRxiv - Microbiology 2019Quote: ... cloned into pGEM-T Easy (Promega), and then inserted using the NotI site ...
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bioRxiv - Microbiology 2020Quote: ... ligated to pGEM-T Easy (Promega), and transformed into E ...
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bioRxiv - Molecular Biology 2021Quote: ... or pGEM-T-Easy vector (Promega). For cloning into pGEM-T-Easy vector ...
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bioRxiv - Plant Biology 2022Quote: ... ligated into pGEM-T (Promega, USA), and checked by sequencing ...
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bioRxiv - Cell Biology 2023Quote: ... cloned in pGEM-T vector (Promega) and sequenced (Macrogen) ...
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bioRxiv - Microbiology 2023Quote: ... Plasmid pGEM®-T Easy (Promega) was used as a primary cloning vector ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... after which 25 µL assay buffer supplemented with 1:25 furimazine substrate (Promega, N246A) and 1:250 goat anti-rabbit Alexa Fluor 594 was added ...
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bioRxiv - Cell Biology 2023Quote: ... 25 µl CellTiterGlo® (Promega) per well was added and the luminescence was read at 700 nm ...
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bioRxiv - Physiology 2022Quote: ... the PCR products were subjected to T/A cloning (pGEM®-T Vector Systems; Promega, Fitchburg, USA) with subsequent Sanger sequencing (Sanger et al ...
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bioRxiv - Plant Biology 2023Quote: ... The fragments were T-cloned using the pGEM®-T Easy Vector System I (cat A1360, Promega) and plated on X-gal/IPTG plates ...
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bioRxiv - Developmental Biology 2024Quote: ... and cloned into pGEM-T Easy vector using pGEM-T Easy Vector System I (Promega; CAT A1360). Vectors were transformed into Escherichia coli using the heat-shock method ...
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bioRxiv - Immunology 2021Quote: ... 25 μL of the reaction mixture was added to 25 μL of CellTiter-Glo (Promega). Luminescence was measured after 10 min using a 0.5 s integration time.
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bioRxiv - Immunology 2021Quote: ... 25 μL of the reaction mixture was added to 25 μL of CellTiter-Glo (Promega). Luminescence was measured after 10 min using a 0.5 s integration time ...
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bioRxiv - Molecular Biology 2023Quote: ... 25 µL of lysate was then mixed with 25 µL of ONE-Glo (Promega # E6120) or 25 µL of prepared Nano-Glo Luciferase Assay System (Promega # N1120 ...
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bioRxiv - Microbiology 2019Quote: ... followed by cloning into pGEM-T (Promega) and sequencing.
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bioRxiv - Evolutionary Biology 2020Quote: ... or pGEM-T Easy Vector (A1360, Promega) (pax9 ...
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bioRxiv - Developmental Biology 2020Quote: ... cloned into pGEM-T Easy vector (Promega) and validated by sequencing ...
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Neurotransmission and neuromodulation systems in the learning and memory network of Octopus vulgarisbioRxiv - Neuroscience 2021Quote: ... ligated into p-GEM T vector (Promega) and linearized with the appropriate restriction endonucleases ...
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bioRxiv - Molecular Biology 2020Quote: ... cloning into PGEM T-easy vector (Promega) and transformation in E ...
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bioRxiv - Molecular Biology 2020Quote: ... then cloned into pGEM-T vector (Promega), to reveal the nucleotide sequence of each clone by Sanger sequence ...
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bioRxiv - Molecular Biology 2019Quote: ... inserted into pGEM T-Easy vector (Promega) and sequenced for determination of SHIP2 genotype.
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bioRxiv - Synthetic Biology 2020Quote: ... An adapted version of pGEM-T (Promega) was used to build the Step 1 crRNA and sgRNA cloning vector ...
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bioRxiv - Neuroscience 2019Quote: ... and ligated into pGEM-T (Promega protocol). Ligations were transformed into JM109 cells (Promega) ...
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bioRxiv - Genetics 2020Quote: ... ligated into pGEM-t-easy vector (Promega) and transformed into DH5α cells ...
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bioRxiv - Neuroscience 2021Quote: ... and cloned into pGEM-T vector (Promega), using the following primers ...
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bioRxiv - Cancer Biology 2020Quote: ... sub-cloned into pGEM-T Easy (Promega) and 4 or more clones per fragment were sequenced using standard Sanger sequencing ...
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bioRxiv - Microbiology 2021Quote: ... and ligated to pGEM-T Easy (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: ... and a poly-T oligonucleotide primer (Promega) following the manufacturer’s instructions ...