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Citations for Promega :
1 - 50 of 3653 citations for SpectraDye Antibody Labeling Kit 550 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: ... Secondary antibody labeling was done using anti-rabbit (Promega 4011) or anti-goat (Jackson Immunoresearch 705-035-147 ...
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Cytoplasm localized ARID1B promotes oncogenesis in pancreatic cancer by activating RAF-ERK signalingbioRxiv - Cell Biology 2019Quote: Halo tag (Halo pulldown and labeling kit, Promega Corporations) and Streptavidin bead (GE Health care Bio-Science ...
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bioRxiv - Cell Biology 2020Quote: ... and gene-body labeling was performed using the Prime-A-Gene kit (Promega).
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bioRxiv - Genomics 2023Quote: ... We used the HaloTag Mammalian Pull-Down and Labeling Systems kit (Promega; #G6500) to identify protein interacting with IFNAR2 in stimulated and unstimulated conditions following manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... Secondary antibody labeling was completed using anti-rabbit IgG-HRP conjugate (1:10,000; Promega W401B) or anti-mouse IgG-HRP conjugate (1:10,000 ...
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bioRxiv - Microbiology 2023Quote: ... Secondary antibody labeling was completed using anti-rabbit IgG-HRP conjugate (1:10,000; Promega W401B) by incubating membranes for 2 h at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: ... and 250,000 cells were co-transfected with PB constructs (550 ng) and pBase plasmid (550 ng) using FuGENE HD transfection reagent (3.9 μl) (Promega), following the manufacturer’s instruction ...
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bioRxiv - Plant Biology 2023Quote: ... The probes were then produced by using the Prime-A-Gene labeling kit (Promega) according to the manufacturer’s instructions.
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bioRxiv - Developmental Biology 2023Quote: ... and were co-transfected with PB constructs (550 ng) and pBase plasmid (550 ng) using FuGENE HD Transfection (Promega E2311), following the protocol for reverse transfection ...
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bioRxiv - Plant Biology 2022Quote: ... purified with a NucleoSpin® Gel & PCR Clean-up kit (Machery-Nagel) and radiolabeled dCTP-P32 were incorporated with a Prime-a-gene Labeling System kit (U1100, Promega). Oligonucletotide probes were ordered from GenoScreen and radiolabeled dATP-P32 were incorporated with a T4 Polynucleotide Kinase kit (T4 PNK EK0031 ...
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bioRxiv - Immunology 2020Quote: ... TUNEL (terminal deoxynucleotidyl transferase deoxyuridine triphosphate nick-end labeling) staining was performed using the DeadEnd kit (Promega, PRG7130) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... alternative HaloTag labeling using Alexa660-HTL (Promega G8471) at 3.5 µM concentration did not generate a sufficient labeling outcome.
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bioRxiv - Microbiology 2020Quote: ... The radioactive DNA probes used were prepared using Prime-a-Gene Labeling System kit (U1100, Promega, Madison, WI, USA), and radiolabeled dCTP (PerkinElmer ...
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bioRxiv - Immunology 2022Quote: Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining was carried out with commercial kit (Promega, Madison, WI, USA) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... the FluoroTect− GreenLys in vitro Translation Labeling System (Promega) was used ...
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bioRxiv - Molecular Biology 2021Quote: ... the two blots were hybridized O/N at 65 °C to each their radioactively labeled 3’ CF probe (AGO1 3’ CF and CSD2 3’ CF probes labelled with Prime-a-gene labeling kit from Promega). The membranes were washed 3 times in 2xSSC (0.3 M NaCl ...
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bioRxiv - Molecular Biology 2021Quote: ... pancreatic sections were submitted to terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) assay using a DeadEnd Fluorometric TUNEL system kit (Promega) according to the manufacturer’s instructions ...
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Glycogen Synthase Kinase 3 regulates the genesis of the rare displaced ganglion cell retinal subtypebioRxiv - Neuroscience 2021Quote: ... Apoptosis was detected by terminal deoxynucleotidyl transferase-mediated biotinylated UTP nick end labeling (TUNEL) assays using in situ cell death detection kit (Promega). All images were acquired using a Zeiss LSM710 confocal microscope and Zen software (Zeiss).
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bioRxiv - Plant Biology 2022Quote: ... Hybridization probes were generated by PCR amplifi cation using gene specifi c primers (Table S1) and radiolabeled with the Prime A Gene labeling kit (Promega) following the recommendations of the manufacturer.
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bioRxiv - Immunology 2023Quote: In situ nick-end labeling of nuclear DNA fragmentation was performed with a TUNEL apoptosis detection kit (DeadEnd Fluorometric TUNEL system, Promega) according to the supplier’s instructions.
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bioRxiv - Cell Biology 2024Quote: ... a labeling solution containing 100 nM JF646-HTL (Promega GA1121) in phenol red-free culture medium was freshly prepared ...
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bioRxiv - Cell Biology 2021Quote: TdT-mediated dUTP nick-end labeling (TUNEL) or EdU assays were performed by using in situ cell death detection kit (TUNEL, Promega Corporation, G3250) or EdU Click-iT® Assay Kit obtained from Thermo Fisher (C10639) ...
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bioRxiv - Molecular Biology 2023Quote: Genomic DNA was extracted from Tetrahymena WT (SB210, with or without BrdU-labeling) and ΔAMT1 cells using Wizard® Genomic DNA Purification Kit (Promega, A1120), sheared to 3-5kb in length with Megaruptor (Diagenode Diagnostics) ...
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bioRxiv - Cell Biology 2021Quote: ... we first performed live-labeling with Halo-JF646 (1:1000, Promega) for 15 minutes at RT ...
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bioRxiv - Microbiology 2022Quote: ... after labeling in-solution digested (sequencing-grade modified porcine trypsin, Promega) immunoprecipitation product with iTRAQ reagent ...
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bioRxiv - Cell Biology 2021Quote: ... microdialysed and 32P-labeled using Prime-a-Gene® Labeling System (Promega). Filters were prehybridized for 2 hr at 65°C in Rapid-hyb solution (GE Healthcare) ...
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bioRxiv - Cell Biology 2022Quote: ... terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL, Promega, Madison, WI, USA), immunohistochemistry (IHC) ...
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bioRxiv - Biophysics 2023Quote: Labeling reactions: Proteins with HaloTag were labeled with JF646-HaloTag ligand (Promega # GA1120) by incubating the proteins with a tenfold excess of dye on ice for 0.5-1 h in the presence of 1 mM ATP ...
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bioRxiv - Cell Biology 2020Quote: ... using the DeadEnd Colorimetric TUNEL (Terminal transferase-mediated dUTP nick-end labeling) system (Promega).
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bioRxiv - Cell Biology 2019Quote: ... or Atto 550 (MolBi Tech)) were delivered into the nucleus via transfection using the FuGENE 6 reagent (Promega), while EdU (Life Technologies) ...
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bioRxiv - Microbiology 2022Quote: ... Then fluorescence signal (excitation 475 nm, emission 500-550 nm) was measured with a microplate reader (Glomax Discover, Promega).
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bioRxiv - Bioengineering 2021Quote: The DeadEnd Colorimetric TUNEL (TdT-mediated dUTP Nick-End Labeling) System (Promega Madison, WI, Cat#G7132) was used to detect apoptosis as per manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... Probes were labeled with α-32P by random priming using Prime- a-Gene Labeling System (Promega), and the radioactive probes were purified using CHROMA SPIN+TE-100 columns (Clontech) ...
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bioRxiv - Microbiology 2022Quote: ... Fluorescent signal (excitation at 475 nm, emission at 500-550 nm) was measured with a microplate reader (Glomax Discover, Promega).
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bioRxiv - Microbiology 2022Quote: ... both OD600nm and fluorescent signal (excitation at 475 nm, emission at 500-550 nm) were measured with a microplate reader (Glomax Discover, Promega).
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bioRxiv - Plant Biology 2022Quote: ... The coding sequence of nLUC (aa 1-416) and cLUC (aa 398-550) were amplified from pSP-LUC+NF (Promega) and inserted between ApaI and XbaI in pSAT1A-cYFP-N1 (ABRC ...
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bioRxiv - Cell Biology 2020Quote: ... cells were stained with different concentrations of TMR HaloTag ligand according to the HaloTag labeling protocol (Promega) and 50,000 cells were analyzed by flow cytometry (BDLSR Fortessa ...
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bioRxiv - Biochemistry 2021Quote: ... Aliquots of reagent were used for sample labeling of a serial dilution of K562 peptide digest standard (Promega) using all heavy N labeled channels that day following manufacturer instructions ...
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bioRxiv - Genetics 2021Quote: ... dCTP-Cy3 (GE) was incorporated along with standards dNTPs for visualization purposes using random priming (Labeling 5X Buffer Promega). For the fixation ...
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bioRxiv - Molecular Biology 2021Quote: Centromeric probe for Southern was produced by apha-32P-dCTP-labelling (Prime-a-Gene Labeling System, Promega cat #U1100) of a ∼300 bp PCR product obtained with primers 5′-CAGAAACTTCTTTGTGATGTGTGC-3′ and 5’-GTTTTTATGGGAAGATATTTCCT-3’ on a template of human genomic DNA.
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bioRxiv - Molecular Biology 2020Quote: ... Paraffin tissue sections mounted on glass slides were used for terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL, Promega) and 4’,6-diamidino-2-phenylindole (DAPI ...
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bioRxiv - Developmental Biology 2020Quote: ... Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) TUNEL assays were performed with the Deadend Fluorometric TUNEL system (Promega), according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... were transfected with 320 ng 12x MS2-tagged repeat expression construct and 80 ng CMV-BFP plasmid DNA (for labeling transfected cells) mixed with 1.6 μl FuGENE HD (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: Terminal deoxynucleotidyl transferase dUTP nick end-labeling (TUNEL) staining was assessed using the DeadEnd Fluorometric TUNEL System (Promega #G3250) per the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... a radioactive probe was made using 50 ng of linearized pCtrl and the Prime-a-Gene Labeling System (Promega) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... viable cells were quantified in a FLUOstar OPTIMA ELISA reader (550/590 nm) about 2 h after CellTiter-Blue staining (Promega, Cat No. G8081). Mean values +/-standard deviations (SD ...
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bioRxiv - Developmental Biology 2020Quote: ... The in situ detection of fragmented genomic DNA was performed according to the DeadEnd colorimetric TUNEL (Terminal transferase-mediated dUTP nick-end labeling) system (Promega). Briefly ...
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bioRxiv - Cell Biology 2022Quote: Digoxin-labeled RNA probes were in vitro transcribed as per manufacturers’ instructions (DIG RNA labeling Mix, Roche; T7 polymerase, Promega; SP6 Polymerase, Promega) and purified by G-50 column (GE Healthcare) ...
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bioRxiv - Cell Biology 2023Quote: ... Terminal deoxynucleotidyltransferase-mediated deoxyuridine triphosphate nick-end labeling (TUNEL) staining of paraffin-embedded tissue sections was done with the DeadEnd Fluorometric TUNEL System (G3250, Promega). TUNEL-positive areas from three fields (magnification×10 ...
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bioRxiv - Molecular Biology 2023Quote: ... siRNAs were detected with a YFP long probe (primers listed in Supplemental Table 2) labeled with 32P-dCTP prepared according to the manual of the Prime-a-Gene Labeling System (Promega). The blot was hybridized overnight at 42 °C with the probe before being washed with 2xSSC ...