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Citations for Promega :
1 - 50 of 3383 citations for S Adenosyl Methionine SAMe CLIA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... 25 μM amino acids minus methionine (Promega), 1 μM Ipom-F ...
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bioRxiv - Microbiology 2022Quote: ... 25 μM amino acids minus methionine (Promega), 6.5% (v/v ...
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bioRxiv - Cell Biology 2022Quote: ... [35S]-methionine-labelled proteins were transcribed and translated using a TnT™ coupled transcription–translation kit (Promega) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: ... and 10 μM methionine-free amino acid mixture (Promega). The Click-iT reaction was performed with the Click-&-Go Protein Reaction Buffer Kit (Click Chemistry Tools ...
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bioRxiv - Bioengineering 2021Quote: ... for radiography detection or with untagged Methionine (Promega #L118A) and Green Lysine (FluoroTect™ GreenLys ...
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bioRxiv - Cell Biology 2021Quote: ... 14 μM amino acid mix w/o methionine (Promega)) ...
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bioRxiv - Cell Biology 2024Quote: 35S-methionine-labelled prey proteins (Grip91, Sas4) were produced in vitro using the TnT T7 Quick Coupled IVTT kit (L1170, Promega). The detailed protocol of the GST-IVTT binding assay is described previously 52.
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bioRxiv - Systems Biology 2020Quote: ... The same amount of trypsin (Promega) was then added (1:10 enzyme to protein ratio ...
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bioRxiv - Microbiology 2020Quote: ... same volume of luciferase detecting regents (Promega) was added to each well ...
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bioRxiv - Immunology 2021Quote: ... The same volume of Steady Glo (Promega) was added to the cells and incubated for 15 min ...
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bioRxiv - Molecular Biology 2023Quote: ... and 1 μl of premix [100 μM amino acid mixture minus methionine (Promega), 100 μM amino acid mixture minus leucine (Promega) ...
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bioRxiv - Molecular Biology 2024Quote: ... and 10 μl of premix [100 μM amino acid mixture without methionine (Promega), 100 μM amino acid mixture without leucine (Promega) ...
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bioRxiv - Microbiology 2020Quote: ... then labeled with 35S-Methionine (Amersham Pharmacia Biotech) in vitro transcription-translation system (Promega), respectively ...
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bioRxiv - Biochemistry 2021Quote: ... complete and minus Methionine and Cysteine were from Promega (cat# L4461 and L5511, respectively); Ambion mMESSAGE mMACHINE™ T7 Transcription kit was purchased from Thermo Fisher Scientific (cat# AM1344) ...
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bioRxiv - Molecular Biology 2023Quote: Cardiomyocytes isolated from WT and Rpl3l-/- hearts were diluted to same concentrations and the cell suspensions were mixed with the same volume of CellTiter-Glo (Promega, G7570), as instructed by the manufacturer ...
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bioRxiv - Cell Biology 2022Quote: ... Protein synthesis was performed in the presence of [35S]methionine in rabbit reticulocyte lysate (Promega). Coupled translation/translocation reactions of 20 μl contained 16.5 μl of reticulocyte lysate ...
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bioRxiv - Biochemistry 2023Quote: Proteins labeled with [35S]-methionine were produced by coupled transcription and translation reactions (T7, Promega). Translation products were partially purified by ammonium sulfate precipitation [87] ...
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bioRxiv - Microbiology 2022Quote: ... The same volume of luciferase Bright-Glo substrate (Promega) was added before acquisition of luminescence on a Wallac Victor2 1420 Multilabel counter (Perkin Elmer system ...
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bioRxiv - Neuroscience 2023Quote: ... The same volume of 0.2 % ProteaseMAX (Promega, Cat# V2072) solution in ABC buffer was added and incubated for an hour with vortex ...
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bioRxiv - Biophysics 2020Quote: ... The cell viability at the same wells was determined by using CellTiter-Glo Luminescent Cell Viability Assay Kit (# G7571, Promega). Neutralization efficiency was calculated as relative fluorescence to the conditioned media collected from mock-transfected cells.
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bioRxiv - Immunology 2022Quote: ... was mixed with the same volume of Fugene® (Promega) in a small volume of Opti-MEM™ reduced serum medium (Invitrogen ...
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bioRxiv - Biochemistry 2023Quote: ... The methyltransferase reaction product S-adenosylhomocysteine was quantified using MTase-Glo kit (Promega, Wisconsin, USA) following the manufacturer’s protocol (43) ...
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bioRxiv - Bioengineering 2024Quote: ... The Glucose-Glo and Lactate-Glo kits (Cat #’s J6021 and J5021, Promega, Madison, WI) were used to measure the apparent glucose and lactate concentrations in media samples according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: Proteins labeled with [35S]-methionine were produced by coupled transcription and translation reactions (SP6 or T7, Promega). Translation products were partially purified by ammonium sulfate precipitation (Bardwell & Shah ...
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bioRxiv - Immunology 2021Quote: ... and used to generate 35S-methionine labelled AIM2 protein by in vitro transcription and translation (IVTT) (Promega). Immunoprecipitations (IP ...
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bioRxiv - Microbiology 2021Quote: ... 35S methionine-labeled full-length cGAS and E5 was generated with the TnT Coupled Reticulocyte Lysate System (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... added same volume of CellTiter-Glo® Luminescent Cell Viability Assay reagent (Promega), and luciferase activity was measured using a BioTek Cytation 3 quipped with Gen5 software (v3.08).
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bioRxiv - Molecular Biology 2020Quote: ... ATFS-11-100(R/R)::GFP and Su91-69::GFP) were synthesized in reticulocyte lysate in the presence of [35S]methionine (T7 Quick for PCR DNA, Promega). Import into isolated mitochondria was performed in import buffer (3 % (w/v ...
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bioRxiv - Biochemistry 2022Quote: Radiolabeled proteins were synthesized in the presence of 35S-methionine and 35S-cysteine using either coupled reticulocyte lysate system (TNT, Promega) or uncoupled reticulocyte lysate system (Promega) ...
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bioRxiv - Developmental Biology 2023Quote: ... Oct4 was transcribed and translated in vitro in the presence of 35S-methionine (TNT Coupled Reticulocyte Lysate System, Promega, USA) according to the manufactureŕs protocol ...
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bioRxiv - Cell Biology 2024Quote: ... were cloned into the pSP64 in vitro translation vector and translated with a 35S radiolabelled methionine label using the TNT coupled reticulocyte lysate system (Promega) following manufacturers guidelines ...
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bioRxiv - Microbiology 2021Quote: ... or α-mouse (C1r/s) (Promega, W402B) antibodies conjugated to horseradish peroxidase ...
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bioRxiv - Microbiology 2023Quote: ... Sequences of all plasmids were verified using a SupreDye v3.1 Cycle Sequencing Kit (M&S TechnoSystems, Cat# 063001) with a Spectrum Compact CE System (Promega). The psPAX2-IN/HiBiT plasmid was a kind gift from Dr ...
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bioRxiv - Microbiology 2023Quote: ... The sequence of the plasmid was verified by sequencing using a SupreDye v3.1 Cycle Sequencing Kit (M&S TechnoSystems, Osaka, Japan, Cat# 063001) with a Spectrum Compact CE System (Promega). To generate a pDON-5 Neo-vector expressing IFNAR1 with the W70C mutation ...
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bioRxiv - Microbiology 2023Quote: ... The plasmid sequence was verified using a SupreDye v3.1 Cycle Sequencing Kit (M&S TechnoSystems, Osaka, Japan, Cat# 063001) with a Spectrum Compact CE System (Promega).
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bioRxiv - Cell Biology 2022Quote: The other in vitro translation was done in the presence of radioactive [35S]methionine using the TNT T7 Reticulocyte Lysate System (Promega 14610). In vitro–translated proteins were then precleared by incubation with empty glutathione Sepharose beads in NETN buffer (50 mM Tris ...
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bioRxiv - Microbiology 2021Quote: ... The reaction mixture was prepared into 96-well plates as follows: 1 μl of 1 mM amino acid mixture minus methionine (Promega, Cat: L9961), 35 μl of rabbit reticulocyte lysate ...
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bioRxiv - Cancer Biology 2022Quote: GSH/GSSG measurement was performed by a glutathione S-transferase (GST) enzyme coupled reaction using (GSH/GSSG Glo kit, Promega). Cells (2×104 ...
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bioRxiv - Microbiology 2023Quote: ... an RNA probe that is the complement to the AS1-S sequence was synthesized from pSPT19 AS1-S using SP6 RiboMAX Large Scale RNA Production System (Promega) and used as a control probe ...
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bioRxiv - Biochemistry 2023Quote: ... Tryptic peptides were generated by the S-Trap Method using S-Strap: Rapid Universal MS Sample Prep (Co2-mini,Protifi) and Trypsin Gold (Promega, V5280). Samples were then vacuum dried and resuspended in 50 μL of 1% formic acid (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2021Quote: ... In vitro transcription and translation to radiolabel the proteins with [35S]-methionine was performed with the TnT® Quick Coupled Transcription/Translation System (Promega, Walldorf, Germany) in reticulocyte lysate ...
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bioRxiv - Systems Biology 2019Quote: ... Both constructs were carried by the same CMV driven mammalian expression vector (pCI-neo derived, Promega) and were maintained as multi-copies within the cells via the presence of the SV40 replication origin.
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bioRxiv - Microbiology 2023Quote: ... following tryptic digest at 37 °C for 3 h in the same buffer with trypsin (Promega) in a 1:30 ratio (w/w ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were resuspended in 1 mL of the same buffer containing protease inhibitor cocktail (Promega, USA), and then transferred to 2 mL lysing matrix B tubes containing 0.1 mm diameter silica beads (MP Biomedicals ...
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bioRxiv - Systems Biology 2023Quote: ... + P/S + 8 ng/mL rhFGF-basic (Promega #G5071) + 2 ug/mL doxycycline ...
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bioRxiv - Biochemistry 2022Quote: ... Digestion was performed for all samples using the same procedure: 500 ng of Sequencing Grade Trypsin (Promega) were added for digestion carried out in a microwave instrument (Discover System ...
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bioRxiv - Systems Biology 2019Quote: ... Both constructs were carried by the same CytoMegaloVirus (CMV)-driven mammalian expression vector (pCI-neo derived, Promega) and were maintained at high copy number via the presence of the SV40 replication origin.
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bioRxiv - Molecular Biology 2021Quote: ... was digested with the same enzymes and ligated with the amplified receptors using T4 DNA ligase (Promega).
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bioRxiv - Plant Biology 2022Quote: ... or with the same buffer supplemented with 5 U of calf intestine alkaline phosphatase (CIAP) from Promega. Samples were incubated at 30°C for 1 h ...
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bioRxiv - Genomics 2022Quote: ... and assessed for quality according to the same protocol described previously (modified SV total RNA isolation system; Promega). RRBS libraries were prepared for 23 samples with 200 ng genomic DNA according to the protocol described previously (Diagenode Premium RRBS kit ...