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Citations for Promega :
1 - 50 of 135 citations for Rubella Spike Glycoprotein E1 strain F Therien since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... Bovine alpha-2-hs-glycoprotein was isolated from plasma (Fetuin, Promega, V4961), and monocyte differentiation antigen CD14 was recombinantly expressed in CHO cells (R&D systems ...
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bioRxiv - Molecular Biology 2023Quote: ... 1ng Luciferase Spike-In RNA (Promega), and 1X protease inhibitor cocktail ...
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bioRxiv - Developmental Biology 2021Quote: ... spike-in λ-DNA (Promega, Cat D150A) was added at a mass ratio of 1/200 ...
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bioRxiv - Biochemistry 2024Quote: ... and 10 units of Peptide N-glycosidase F (PNGase F, Promega) enzymes according to manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2019Quote: ... coli JM109 strain (Promega) was transformed with the violacein pathway plasmid (pTU2S(VioAE)-b-VioBCD ...
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bioRxiv - Cell Biology 2021Quote: ... coli strain KRX (Promega) and cultured in LB medium containing 100 µg/ml ampicillin ...
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bioRxiv - Microbiology 2020Quote: ... coli strains JM109 (Promega) and S17-1λpir/pLFX (E ...
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bioRxiv - Microbiology 2022Quote: 293T cells were co-transfected with pEGFP-N1 and plasmids expressing SARS-CoV-2 Spike (FL) or SARS-CoV-2 Spike-Δ19 for 24 h using FugeneHD (Promega). The cells were treated with DMSO or 10 µM 6-TG at 4 h post- transfection ...
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bioRxiv - Microbiology 2022Quote: ... and a spike-expressing plasmid (pCAGGS-SARS-CoV-2-spike) were co-transfected into HEK293T cells using Fugene HD transfection reagentia (Promega) according to the manufacturer’s guidelines ...
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bioRxiv - Microbiology 2023Quote: Vero E6 cells were transfected with an GFP expression vector together with either a control expression vector (no spike) or a SARS-CoV-2 spike expression vector using Fugene (Promega). Two hours after transfection ...
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bioRxiv - Microbiology 2019Quote: Escherichia coli strain JM109 (Promega) was used for cloning and amplification of plasmids ...
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bioRxiv - Developmental Biology 2021Quote: ... Spike-in RNA (polyadenylated kanamycin mRNA #C1381, Promega, USA) was added to the Cell Lysis Buffer with a final concentration 107 molecules/ml ...
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bioRxiv - Microbiology 2020Quote: ... and 1 μg of a plasmid expressing the VSV G glycoprotein using the FuGene HD reagent (Promega). Forty-eight hours later ...
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bioRxiv - Microbiology 2023Quote: ... and 0.25 μg vesicular stomatitis virus G glycoprotein expression plasmids using the Fugene 6 transfection reagent (Promega) according to the manufacturer’s recommendations ...
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bioRxiv - Neuroscience 2022Quote: ... and pGlosensor F-22 (Promega) were amplified and purified by Nucleospin plasmid TF grade (Takara bio ...
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bioRxiv - Biochemistry 2021Quote: ... chymotrypsin and peptide-N-glycosidase F (PNGase F) were obtained from Promega (Madison, WI, USA). Centrifugal filters (0.5 mL ...
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bioRxiv - Synthetic Biology 2020Quote: ... coli strain BL21 (DE3) pLysS (Promega). A single colony was picked to inoculate a 5 mL starting culture that was allowed to grow overnight at 37 °C with 225 rpm before being diluted into large cultures (1 liter for PhoCl green state and red state ...
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bioRxiv - Microbiology 2024Quote: ... coli strain JM109 (Promega; Madison, WI). JM109 harboring pJBA132 was routinely cultured in Miller’s Luria-Bertani (LB ...
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bioRxiv - Cell Biology 2019Quote: ... CellTiter-Glo and PGNase F (Promega), and Cytotoxicity Detection kitPLUS(Roche Diagnostics ...
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bioRxiv - Molecular Biology 2020Quote: ... and PNGase-F (Promega Cat#V4831) prior to two-dimensional gel electrophoresis ...
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bioRxiv - Developmental Biology 2021Quote: ... purified genomic DNA (with 1% unmethylated lambda DNA spike-in, Promega) was sheared to a fragment size of 100 - 700 bp (primary size 250 bp) ...
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bioRxiv - Microbiology 2020Quote: ... Transformations of the cloning strains DH5α (Promega), NEB 5-alpha (New England Biolabs) ...
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bioRxiv - Microbiology 2022Quote: ... coli strains harboring pNL1.1 (Promega, Madison, WI) (see primers in Table S4 ...
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bioRxiv - Biochemistry 2019Quote: ... in Escherichia coli strain BL21(DE3) (Promega) and purified via glutathione-affinity column chromatography ...
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bioRxiv - Immunology 2020Quote: ... IgG were deglycosylated using PNGase F (Promega) with 0.05 U of PNGase/μg of IgG ...
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bioRxiv - Biochemistry 2022Quote: Samples were treated with PNGase F (Promega) according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: ... N-glycans were released from the intact proteins using 0.5 U/ µL N-glycosidase F (PNGase F, Elizabethkingia miricola, Promega), overnight ...
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bioRxiv - Cell Biology 2022Quote: ... coli strain JM109 (DE3) was purchased from Promega. Liquid growth media and cultures were prepared in acid washed glassware or sterile plasticware to minimise metal contamination ...
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bioRxiv - Biochemistry 2022Quote: ... coli strain JM109 (DE3) was purchased from Promega. Liquid growth media and cultures were prepared in acid washed glassware or sterile plasticware to minimise metal contamination ...
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bioRxiv - Immunology 2020Quote: ... protein samples were treated with PNGase F (Promega) according to manufacturer’s instruction under denaturing conditions ...
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bioRxiv - Microbiology 2022Quote: ... NTD and Spike - was achieved for 24 h using ProFection Mammalian Transfection System (Promega) or adenovirus infection ...
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bioRxiv - Immunology 2021Quote: ... and hFcγRIIIa-F variant (G9791) were purchased from Promega Corporation (Madison ...
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bioRxiv - Immunology 2023Quote: ... Eluted proteins were then treated with PNGase F (Promega) according to manufacturer’s protocol and analyzed with SDS-PAGE ...
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bioRxiv - Microbiology 2021Quote: ... faecium Aus0004 WT strain were circularized using T4RNA ligase (Promega), reverse transcribed by M-MLV RT (Promega ...
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bioRxiv - Microbiology 2021Quote: ... coli strain JW5925-149 using Platinum Taq DNA polymerase (Promega) and primers PC3 and PC4 with specificity for the regions flanking sslE ...
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bioRxiv - Biochemistry 2019Quote: ... The eluted protein was first treated with PNGase-F (Promega) to remove the N-linked glycosylation and then analyzed by mass spectrometry to determine the N-terminal sequence ...
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bioRxiv - Immunology 2023Quote: Following the manufacturer’s instructions for PNGase F (Promega Inc., V483A) treatment ...
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bioRxiv - Microbiology 2019Quote: ... coli strain JM109 single use competent cells were purchased from Promega and used for transformations in E ...
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bioRxiv - Cell Biology 2023Quote: The bacterial expression vectors were transformed into the KRX strain (Promega). GST-tagged protein expression was induced by 0.1% rhamnose ...
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bioRxiv - Microbiology 2022Quote: ... pLJM1-Luc2 used in generating Spike-pseudotyped lentiviruses was generated by cloning Luc2 from pGL4.26 (Promega) into pLJM1-B* (67) ...
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bioRxiv - Cell Biology 2022Quote: The SARS-CoV-2 spike pseudotyped virus activity was determined by bright-glo luciferase assay (Promega). The plate reader detected the luminescence two days post virus infection or without virus infection ...
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bioRxiv - Microbiology 2023Quote: ... the pcDNA3.1+ expression plasmids for spike proteins were co-transfected using Fugene (Promega, Madison, WI, USA) into HEK293T/17 cells ...
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bioRxiv - Microbiology 2020Quote: ... Peptides were released by digestion with PNGase F (Promega, Madison, WI) overnight at 37 °C with vortexing ...
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bioRxiv - Microbiology 2020Quote: ... cells were transfected with 500 ng of either HDM-SARS-Spike-delta21 or HDM_Spike_RBD_B7-1 plasmids and 1.5 ng of Transfection Carrier DNA (Promega, E4881) using BioT reagent (Bioland Sci ...
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bioRxiv - Microbiology 2021Quote: ... Spike+ cells were then single cell sorted into 96-well PCR plates containing RNase inhibitor (N2611; Promega) and stored at −80 °C.
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bioRxiv - Microbiology 2019Quote: ATP levels in strains were determined by BacTitre-GloTM Assay Kit (Promega) as previous described ...
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bioRxiv - Microbiology 2020Quote: ... coli strains was prepared using the Wizard Genomic DNA Purification kit (Promega) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... pSY5M-Pak4cat were introduced into Escherichia coli strain BL21(DE3) pLysS (Promega), and the fusion proteins were induced by incubation with 0.5 mM isopropyl-b-D-thiogalactoside (IPTG ...
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bioRxiv - Microbiology 2023Quote: ... coli strain S17-1λpir and confirmed by colony PCR (GoTaq polymerase, Promega) and Sanger sequencing (Microsynth ...
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bioRxiv - Cancer Biology 2022Quote: ... and N-glycans were released following the addition of PNGase F (Promega) in PBS ...