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Citations for Promega :
1 - 50 of 420 citations for Recombinant Human REN His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2022Quote: ... His-tagged and GST-tagged recombinant proteins were purified using Promega MagneHis™ Ni-Particles (Promega Cat#V854A) and MagneGST™ Glutathione Particles (Promega Cat# V861A ...
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bioRxiv - Biochemistry 2023Quote: ... recombinant GST-tagged MARK2 (Promega) was incubated with tau at 30 °C at 1:100 ratio of MARK2:tau in phosphorylation buffer (25 mM PIPES ...
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bioRxiv - Molecular Biology 2022Quote: ... His-tagged proteins were purified using Ni-NTA resin (Promega); bound proteins were washed with binding buffer and eluted into elution buffer (4 M urea ...
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bioRxiv - Immunology 2023Quote: ... and REN-Luc/pRL-TK plasmid (20 ng; Promega) expressing Renilla luciferase used as an internal control to normalize transfection efficiency ...
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bioRxiv - Molecular Biology 2019Quote: ... 10.0 ng/ml recombinant human FGF (Promega) and 1.0 μM dexamethasone (Sigma-Aldrich) ...
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bioRxiv - Cancer Biology 2023Quote: HEK293T cells were transfected with a FLAG-tagged human DHHC20-expressing plasmid and a corresponding HA-tagged substrate-expressing plasmid using FuGENE transfection reagent (Promega) and incubated overnight ...
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bioRxiv - Genomics 2022Quote: ... 10 ng/mL recombinant human basic fibroblast growth factor (Promega), and 1 μM dexamethasone (Sigma-Aldrich) ...
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bioRxiv - Plant Biology 2020Quote: ... The ratio of LUC/REN activity was measured using the DLR assay system (Promega). Primers are listed in SI Appendix Table S2.
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bioRxiv - Plant Biology 2022Quote: ... LUC and REN activities were measured using the Dual-Luciferase Reporter Assay System (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2021Quote: ... and 5 ng/ml recombinant human fibroblast growth factor (G5071, Promega).
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bioRxiv - Plant Biology 2020Quote: ... we measured the LUC and REN activities using dual-luciferase reporter assay reagents (Promega, USA) and a GloMax 20/20 luminometer (Promega ...
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bioRxiv - Plant Biology 2022Quote: ... LUC and Renilla (REN) luciferase activities were measured using the Dual Luciferase Assay Kit (Promega) and analyzed using the Luminoskan Ascent Microplate Luminometer (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2019Quote: ... and 5 ng/mL recombinant human basic fibroblast growth factor (bFGF, Promega) then centrifuged ...
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bioRxiv - Plant Biology 2022Quote: ... firefly luciferase (LUC) and REN activities were measured using the Dual-Luciferase Reporter Assay System (Promega) following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2021Quote: ... the LUC and REN expression were detected by the Dual-Luciferase Reporter Assay System (Promega, USA). At least six biological replicates were measured ...
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bioRxiv - Biochemistry 2022Quote: ... and firefly luciferase and Ren-Luc activity quantified using the dual-luciferase reporter assay system (Promega). Ren-Luc levels were corrected with the firefly luciferase levels (resulting in standardised relative light units [sRLU] ...
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bioRxiv - Plant Biology 2023Quote: ... Firefly LUC and REN activities were measured using the Dual-Luciferase Reporter Assay System (Promega, E1910) following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... and firefly luciferase and Ren-Luc activity quantified using the dual-luciferase reporter assay system (Promega). Ren-Luc levels were corrected with the firefly luciferase levels (resulting in standardised relative light units [sRLU] ...
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bioRxiv - Plant Biology 2024Quote: ... The LUC and REN luciferase activities were measured using the Modulus luminometer (Promega, Madison, WI, USA).
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bioRxiv - Molecular Biology 2019Quote: ... and analysed for firefly luciferase and Ren-Luc activity using the Dual-Luciferase Reporter Assay System (Promega). To compensate for differences in transfection efficiency or cell density ...
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bioRxiv - Plant Biology 2021Quote: ... the expression of LUC and REN were detected by the Dual-Luciferase Reporter Assay System (Promega, USA). At least six biological replicates were measured ...
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bioRxiv - Biochemistry 2021Quote: ... and firefly luciferase and Ren-Luc activity were quantified using the dual-luciferase reporter assay system (Promega). Ren-Luc levels were corrected with the firefly luciferase levels (resulting in standardized relative light units [sRLU] ...
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bioRxiv - Plant Biology 2023Quote: ... Firefly luciferase (LUC) and Renilla luciferase (REN) activities were measured using dual luciferase assay reagent (Promega, VPE1910) after one day ’co-cultivation in dark and two days in light ...
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bioRxiv - Plant Biology 2024Quote: ... and Firefly (FLUC) and Renilla (REN) luciferase activities were measured using the Dual Luciferase Reporter Assay (Promega) following the addition of 50 µl of each enzyme substrate ...
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bioRxiv - Plant Biology 2021Quote: ... LUC and REN activities were measured by the Dual-Luciferase Reporter Assay System (Promega Corporation, Madison, WI, USA), and LUC activity was normalized against REN activity ...
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bioRxiv - Cell Biology 2022Quote: ... the LUC/REN ratio was measured with the dual-luciferase reporter assay system following the manufacturer’s protocols (Promega). Mean values and standard deviations were calculated from three biological replicates.
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bioRxiv - Molecular Biology 2020Quote: ... 2.5ng ml-1 recombinant human fibroblast growth factor (rhFGF) (Promega, Cat#G5071, Madison, WI, USA), and 1% chicken embryonic extract (Seralab ...
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bioRxiv - Molecular Biology 2023Quote: ... 15 mg of His-Tev-HaloTag-(3C)-human γ-TuNA was coupled to Halo Magne beads (Promega). Xenopus laevis egg extract was prepared by standard methods28 ...
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bioRxiv - Plant Biology 2021Quote: ... The firefly LUC and REN activities luciferase were detected by transient dual-luciferase reporter assay system (Promega, Madison, USA), and LUC/REN ratio was calculated ...
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bioRxiv - Microbiology 2021Quote: ... and both Luciferase (FF-Luc) and Renilla luciferase (REN-Luc) activities were measured as described by the manufacturer (Promega). As pTM-LACV_L ...
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bioRxiv - Plant Biology 2023Quote: ... Luciferase and renillia luciferase (REN, for normalization) activities were detected with a Dual-Luciferase Reporter Assay System (Promega, E1910) under the GloMax Discover plate reader system from Promega ...
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bioRxiv - Cell Biology 2023Quote: ... 10mM Ultrapure ATP and 0.7 μg of recombinant full-length human CDC7/DBF4 kinase (Promega, V5088). Reactions were incubated at 37 °C for 120 min and then terminated by freezing on dry ice before mass spectrometry.
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... cell surface levels of HiBiT-tagged human S1PR1 were monitored using a Nano Glo HiBiT Extracellular Detection System (Promega) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: PS cells were seeded at 1.5 × 105 cells per well in 96-well plates (n=3) and transfected with in vitro transcribed TBEV replicon RNA (100 ng/well) and 10 ng of pTK-Ren (Promega) using Lipofectamine-2000 (ThermoFisher Scientific) ...
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bioRxiv - Plant Biology 2023Quote: ... and the control Renilla (REN) luciferase activities were assayed from leaf extracts with the Dual-Glo Luciferase Assay System (Promega) and quantified with a GloMax-Multi Detection System (Promega).
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bioRxiv - Biochemistry 2023Quote: Transient transfection of the dual NanoLuc and Halo-Tagged tagged BRD4Tandem plasmid (Promega) was performed as described previously52 ...
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bioRxiv - Genetics 2019Quote: ... A cDNA encoding Myc epitope-tagged human TRAPα was synthesized by Integrated DNA Technologies (IDT) and was subcloned into the pTarget mammalian expressing vector (Promega).
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... were co-transfected with pcDNA5/FRT construct encoding haemagglutinin (HA)-tagged human CB1 receptor cDNA and pOG44 (Flp recombinase plasmid) using transfection reagent Fugene HD (Promega) as previously described for AtT-20 pituitary tumour cells [26] ...
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bioRxiv - Biochemistry 2022Quote: ... 1200 ng of ORs tagged with the first 20 amino acids of human rhodopsin (rho-tag) at the N-terminal ends53 in pCI (Promega) and 30 ng eGFP were transfected using Lipofectamine 2000 (Invitrogen ...
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bioRxiv - Molecular Biology 2021Quote: ... The activities of firefly luciferase (LUC) and Renilla luciferase (REN) were measured using a Dual-Luciferase Reporter Assay System (E2920, Promega, USA). The LUC activity was normalized to the REN activity ...
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bioRxiv - Plant Biology 2023Quote: ... Activities of firefly luciferase (LUC) and Renilla luciferase (REN) were examined using a Dual-Luciferase Reporter Assay System kit (Promega, E1960). LUC/REN was calculated as the relative activity and the ratio of dark / HL was used to indicate light response ...
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bioRxiv - Plant Biology 2020Quote: ... The recombinant Halo-His-gene was expressed using a 30 μL TNT®SP6 High-Yield Wheat Germ Protein Expression System (Promega, USA, Catalog No L3261). Expression levels were determined by SDS-PAGE and western blot ...
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bioRxiv - Microbiology 2021Quote: ... HA-tagged DDX1and HA-tagged TRIF were constructed by inserting indicated sequences into pCMV-HA (Promega), respectively ...
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bioRxiv - Neuroscience 2019Quote: ... were transfected with FLAG-tagged Nlgn1 or GFP-tagged NGL-3 using Fugene6 (Promega, Cat #E2691). Transfected HEK293T cells were mechanically dissociated 24 hr after transfection and co-cultured with DIV10 cortical neurons prepared from C57BL/6J Sorcs1flox/flox mice for 16 hr.
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bioRxiv - Microbiology 2022Quote: ... FLAG-tagged or HA-tagged protein was detected using a commercially available mouse monoclonal antibody (Promega).
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bioRxiv - Genetics 2020Quote: ... The corresponding Firefly luciferase (LUC) and Renilla luciferase (REN) values were measured 16 h after transfection using a Dual-Luciferase® Reporter Assay System (Promega, WI, USA).
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bioRxiv - Molecular Biology 2022Quote: ... Transient expression was calculated by determining the ratio of luciferase (LUC) and Renilla (REN) using the Dual-Luciferase® machine (Promega, WI, USA). Additionally ...
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bioRxiv - Plant Biology 2024Quote: ... the LUC and REN values were measured 24 hours later using the Dual-Luciferase® 80 Reporter Assay System (E1910, Promega, WI, USA)34 ...
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bioRxiv - Neuroscience 2023Quote: Nine DIV hippocampal slice cultures were randomly assigned to treatment groups with the following drugs dissolved in serum-free medium: recombinant human BDNF (250 ng/mL, Promega); BDNF + K-252a (200 nM ...
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bioRxiv - Cell Biology 2021Quote: ... Janelia Fluor 646 tagged Halo ligand (Promega) was used at 200nM final concentration to label SINAPS-mRNA in cells prior to fixation/imaging.